Eriodictyol Protects Endothelial Cells against Oxidative Stress-Induced Cell Death through Modulating ERK/Nrf2/ARE-Dependent Heme Oxygenase-1 Expression

Lee, Seung Eun,Yang, Hana,Son, Gun Woo,Park, Hye Rim,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong Seek MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.7

<P>The pathophysiology of cardiovascular diseases is complex and may involve oxidative stress-related pathways. Eriodictyol is a flavonoid present in citrus fruits that demonstrates anti-inflammatory, anti-cancer, neurotrophic, and antioxidant effects in a range of pathophysiological conditions including vascular diseases. Because oxidative stress plays a key role in the pathogenesis of cardiovascular disease, the present study was designed to verify whether eriodictyol has therapeutic potential. Upregulation of heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, in endothelial cells is considered to be helpful in cardiovascular disease. In this study, human umbilical vein endothelial cells (HUVECs) treated with eriodictyol showed the upregulation of HO-1 through extracellular-regulated kinase (ERK)/nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathways. Further, eriodictyol treatment provided protection against hydrogen peroxide-provoked cell death. This protective effect was eliminated by treatment with a specific inhibitor of HO-1 and RNA interference-mediated knockdown of HO-1 expression. These data demonstrate that eriodictyol induces ERK/Nrf2/ARE-mediated HO-1 upregulation in human endothelial cells, which is directly associated with its vascular protection against oxidative stress-related endothelial injury, and propose that targeting the upregulation of HO-1 is a promising approach for therapeutic intervention in cardiovascular disease.</P>

韓國 四森林帶의 소나무林에 관한 植物社會學的 硏究

林暎得,姜鎬監,中越信和 仁川敎育大學校 1991 論文集 Vol.25 No.2

Four climatic forest zones were set up for the investigated areas in South Korea. A phytosociological study showed little differences in C, N and C/N ratio except for available phosphorus and pH. A correlation between DBH and tree height showed a similar tendency of DBH and age in all investigated areas. The nerer the villages, more human impact on vegetation was observed. In addition, men's impacts were considered to be a significant environmental foctor in growth rate of P. densiflora populations.

제주도 지하수 질산염 농도의 시·공간적 변화 특성: 장기(1993-2015) 모니터링 자료의 평가

김호림(Ho-Rim Kim),오준섭(Junseop Oh),도현권(Hyun-Kwon Do),이경진(Kyung-Jin Lee),현익현(Ik-Hyun Hyun),오상실(Sang-Sil Oh),감상규(Sang-Kyu Kam),윤성택(Seong-Taek Yun) 대한자원환경지질학회 2018 자원환경지질 Vol.51 No.1

1993년부터 2015년까지 관측된 제주도 지하수 장기모니터링 관측정(N = 4,835)에서 수집된 지하수 수질자료(N = 21,568)를 기반으로 질산성질소의 시공간적 변동 특성을 평가하였다. 제주도 지하수의 질산성질소 농도의 중앙값은 2.5 mg/L로서 다른 국가나 대륙의 조사 결과에 비해 다소 높거나 유사한 것으로 나타났다. 또한 지하수 용도, 행정구역 및 고도 별로 유의한 차이를 보였다. 특히, 산간 지역에 비해 저지대 해안가에 위치한 농업 및 주거지역에서 농도가 높음을 확인하였다. Mann-Kendall 및 Sen’s slope 분석을 활용한 질산성질소 농도의 추세 분석 결과, 하류 저지 대에 비해 중산간지역에서의 질산성질소 농도 증가 경향이 뚜렷하였다. 제주도 내 토지 피복의 시계열 변화 특성과 결부 지어 보면, 중산간지역의 오염 증가 추세는 농업지역의 확장 등 인위적 활동 증가에 기인한 결과로 판단된다. 반면,기지정된 지하수자원특별관리구역에서는 전반적으로 질산성질소 농도의 감소 경향이 나타났는데, 이는 지하수 관리 측면에서 수질관리를 위한 적극적인 정책이 유효함을 시사한다. 본 연구에서는 제주도 지하수의 질산성질소 오염관리를 위한 적정 방안을 제안한다. The spatio-temporal variations of nitrate concentrations in groundwater of Jeju Island were evaluated by an analysis of time series groundwater quality data (N = 21,568) that were collected from regional groundwater monitoring (number of wells = 4,835) for up to 20 years between 1993 and 2015. The median concentration of NO 3 -N is 2.5 mg/L, which is slightly higher than those reported from regional surveys in other countries. Nitrate concentrations of groundwater in wells tend to significantly vary according to different water usage (of the well), administrative districts, and topographic elevations: nitrate level is higher in low-lying agricultural and residential areas than those in high mountainous areas. The Mann-Kendall trend test and Sen’s slope analysis show that nitrate concentration in mid-mountainous areas tends to increase, possibly due to the expansion of agricultural areas toward highland. On the other hand, nitrate concentrations in the Specially Designated Groundwater Quality Protection Zones show the temporally decreasing trend, which implies the efficiency of groundwater management actions in Jeju. Proper measures for sustainable groundwater quality management are suggested in this study.

Crotonaldehyde-exposed macrophages induce heme oxygenase-1 expression as an adaptive mechanism

Seung Eun Lee,Hana Yang,Gun Woo Son,Hye Rim Park,Young-Ho Jin,Cheung-Seog Park,Yong Seek Park 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Cigarette smoke represents one of the most significant risk factors for the progression of vascular disease. Exposure to cigarette smoke induces oxidative stress in the vascular system and results in vascular dysfunction. Crotonaldehyde, a highly reactive α,β-unsaturated aldehyde, is a foremost compound of cigarette smoke and a product of endogenous lipid peroxidation. Heme oxygenase-1 (HO-1) expression plays an essential role in cellular defense against environmental insults and represents an adaptive response. Here, we showed the effects of crotonaldehyde on the induction of HO-1 expression in RAW 264.7 macrophages. Crotonaldehyde treatment resulted in significantly increased phosphorylation of p38 mitogenactivated protein kinase (MAPK) and nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2). Furthermore, treatment with zinc protoporphyrin (ZnPP; the specific HO-1 inhibitor) markedly augmented the death of crotonaldehyde-treated macrophages. In summary, these results highlight the role of the HO-1 upregulation through p38 MAPK-Nrf2 activation in the adaptive response to crotonaldehyde in macrophages.

The Antioxidant Effect of the Rhaponticum uniflorum Extracts on the Oxidative Stress in H₂O₂ induced Human Keratinocytes

So Hee Ahn,Won Yeoung Choi,Ji Won Jeong,Geon Woo Park,Su Yeon Kang,Eun Jeong Kim,Su Rim Sin,Eun Seo Jang,Gye Won Lee,Young Ho Cho 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, To investigate the antioxidant activities of Rhaponticum uniflorum extracts (RUE), we assessed scavenging activities on DPPH, ABTS radicals, and reducing power. The DPPH and ABTS radical scavenging activities (SC50) of RUE were 96.97±1.19 μg/mL and 31.26±0.13 μ g/mL, respectively, and the reducing power significantly increased dose-dependent manner. To study the antioxidant effecters of RUE in H₂O₂ induced human keratinocytes, expression of Nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot and qRT-PCR. RUE induced the expression of HO-1, a critical target enzyme of Nrf2, in a concentration-dependent manner. The expression of HO- 1 mRNA was also induced in H₂O₂-treated HaCaT cells but no significant difference in the level of HO-1 mRNA was observed. Taken together, these results suggest that RUE may be useful as a potential ingredient for anti-aging products.

급성 복증 소견을 보인 신증후출혈열 1예

신호식,정연순,임학 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Hemorrhagic fever with renal syndrome (HFRS) frequently cause abdominal pain with tenderness; therefore this is occasionally misdiagnosed as surgical abdomen. A 20-year-old male presented with abdominal pain for 2 days. He also had suffered from fever since 7 days ago. Abdominal computed tomography showed ascites along both paracolic gutters, mesentery and paravesical spaces. Hantaan virus infection was demonstrated by serology. After conservative treatment, the patient with HFRS spontaneously improved. We report a case of HFRS presented acute focal abdominal pain of acute abdomen.

豚 및 山羊 乳頭의 組織學的 考察

林鳳鎬,朴南鏞,金五南 全南大學校 農漁村開發硏究所 1977 農業科學技術硏究 Vol.12 No.-

豚의 離乳直後期의 乳頭, 泌乳靜止期의 乳頭 및 閉乳期의 乳頭와 山羊의 靜止期乳頭를 組織學的으로 考察하고 比較 檢討하였던 바 그 結果는 다음과 같다. 1. 豚, 山羊의 乳頭 斷面은 大略 皮膚인 外層과 結合組織, 平滑筋織雜 및 血管 등으로 構成되어 있는 中間層과, 乳管洞과 粘膜下組織으로 되어 있는 中心層, 즉 三層으로 構成되어 있다. 2. 豚, 泌乳靜止期 乳頭는 離乳直後期 乳頭에 比해 結合組織의 增殖, 若干의 乳腺房의 退化가 있을 뿐 別 差異를 認定할 수 없었다. 閉乳期의 乳頭는 全體的인 萎縮現像이 보였고, 中間層은 基底部에 이르면서 結合組織 漸次 脂肪組織으로 轉換되어 있었다. 3. 山羊의 乳頭에서는 毛包, 皮脂腺, 大汗腺이 分布하고 있었으나 豚의 乳頭에서는 오직 分泌腺으로서는 小汗腺 뿐이었다. 4. 乳管洞은 豚에서 2個, 山羊에서 1個였으며, 豚의 乳管洞 上皮細胞는 重層立方上皮 細胞였고 山羊은 單列, 또는 2列로 配列되어 있는 圓柱上皮였다. 乳管洞은 兩者 共히 많은 추벽 이 있었으며, 離乳直後期의 경우 이 추벽은 더욱 發達하였고 閉乳期의 것은 粘膜下 組織의 增殖에 따라 突出의 程度가 其하지 안했다. 5. 豚이나 山羊에서는 사람의 Montgomery's 腺같은 乳輪腺은 發見되지 안했다. 6. 脂肪組織은 閉乳其의 豚乳頭 以外에 發見되지 안했다. 7. 乳頭의 中間層은 皮膚에서는 皮下組織, 中心層의 粘膜層에서는 粘膜下 組織에 該當되는 部位이며, 縱走나 輪走하는 平滑筋織雜와 두꺼운 壁의 動靜脈이 農富해서 乳頭의 收縮 膨脹과 關係있는 것으로 思料되었다. The teats of swine and goat were examined microscopically. Three stages of swine teat wee classified ; shortly after weaning, during resting lactation, and during involution. the teats of goat were examined only during resting lactation. The results were as follows : 1. The teats of swine and goat consisted of three layers; skin, the fibromuscular vascular middle layer, and layer of the mucosa of the lactifererous duct and canal. 2. The teats of goats possessed hair follicle, sebaceous gland and sweat gland(Glandulae sudoriferae), in contrast with these only the sweat gland was found in the teat of swine. 3. The teats of swine had two lactiferous ducts, but the goat had only one duct. The epithelium of the mucous membrane of swine lactiferous sinus was stratified cuboidal cells, and that of goat was one or two layer columnar cells. The lactiferous sinuses of swine and goat possesed more or less longitudinal folds that project to the lumen at various stages. 4. The fibromuscular vascular middle layer consisted of the connective tissues, circular and longitudinal smooth muscle fiber, and the abundant blood vessels which have thick walls. 5. No fat tissue was found in the teat of swine or goat except for the swine teats of involution. 6. In the areola, the gland known as the Montgomery's gland which is found in human was not found in either swine or goat.

혈청 Cystatin C와 크레아티닌에 근거한 사구체 여과율의 비교

신호식,정연순,임학 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background : In recent years, cystatin C (CysC) was proposed as a new marker for the glomerular filtration rate (GFR) due to a constant serum level. The present study analyzes whether creatinine based GFR prediction equations might be replaced by simple prediction equations based on serum concentrations of CysC in healthy young Korean men. Methods : We studied 145 apparently healthy young Korean men aged 19-29 years who visited The Armed Forces Yangju Hospital (Gyeonggi Province, Repulic of Korea) for health check from November 2008 to January 2009. Creatinine-based GFR was estimated by the formula of modified MDRD, Crockcroft Gault (CG). CysC-based GFR was estimated by the formula of Larsson, Hoek, Filler, Grubb, Le Bricon, Orebro-cyst (DAKO), Orebro-cyst (Gentian). Linear regression analysis was performed to evaluated correlations between GFR estimating formulae based upon serum CysC and serum creatinine. Results : Among CysC based GFR, Hoek's GFR is most similar with CG GFR and abbreviated MDRD GFR. Significant correlations were noted between CysC based GFR and creatinine based GFR. Conclusion : Creatinine based GFR prediction equations for healthy young Korean men might be replaced by simple prediction equations based on serum concentrations of CysC (especially Hoek' equation). 배경 : 혈청 cystatin C는 외부 요인에 영향을 받지 않고 일정한 값을 유지하므로 사구체 여과율에 대한 새로운 표지자로 제시되고 있다. 본 연구는 젊은 성인 남성에서 cystatin C에 근거한 사구체 여과율이 혈청 크레아티닌에 의한 사구체 여과율을 대체할 수 있는지 조사하였다. 방법 : 2008년11월부터 2009년1월까지 병원을 방문한 평균 연령 21세의 젊은 성인남성 145명을 대상으로 하였다. Cystatin C에 근거한 사구체 여과율은 Larsson, Hoek, Filler, Grubb, Le Bricon, Orebro-cyst (DAKO), Orebro-cyst (Gentian) 공식을 이용하였고 혈청 크레아티닌에 근거한 사구체 여과율은 변형된 MDRD, Crockcroft Gault 공식을 이용하여 산출하였다. Cystatin C에 근거한 사구체 여과율과 혈청 크레아티닌에 근거한 사구체 여과율은 선형 회귀분석을 통해서 연관 관계를 확인하였다 결과 : Cystatin C에 근거한 사구체 여과율 산출 공식중에서 Hoek 공식에 의한 사구체 여과율이 혈청 크레아티닌에 근거한 사구체 여과율과 의미있는 상관관계를 보였다. 결론 : 젊은 성인 남성에서 Cystatin C에 근거한 사구체 여과율은 혈청 크레아티닌에 의한 사구체 여과율을 대체할 수 있을 것으로 생각된다.

하악 대구치 Ⅱ형 근심 근관에서 치근단 부위의 만곡도 조사

윤혜림,이동균,황호길 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.2

Objectives: The purpose of this study was to evaluate the buccolingual curvature at the apical one third in type Ⅱ mesial canals of mandibular molars using the radius and angle of curvature. Materials and Methods: Total 100 mandibular molars were selected. Following an endodonticaccess in the teeth, their distal roots were removed. #15 H- or K-files (Dentsply Maillefer) were inserted into the mesiobuccal and mesiolingual canals of the teeth. Radiographs of the teeth were taken for the proximal view. Among them, type Ⅱ canals were selected and divided into two subgroups, Ⅱa and Ⅱb. In type Ⅱa, two separate canals merged into one canal before reaching the apex and in type Ⅱb, two separate canals merged into one canal within the apical foramen. The radius and angle of curvature of specimens were examined. Results: In type Ⅱ, mean radius of curvature in mesiolingual and mesiobuccal canals were 2.82 mm and 3.58 mm, respectively. The radius of the curvature of mesiolingual canals were significantly smaller than that of mesiobuccal canals in type Ⅱ, and especially in type Ⅱa. However, there were no statistically significant differences in radius of curvature between mesiobuccal and mesiolingual canals in type Ⅱb and there were no significant differences in angle of curvature between type Ⅱa and Ⅱb. Conclusion: In this study, type Ⅱ mesial canals of mandibular molars showed severd curvature in the proximal view. Especially, mesiolingual canals of type Ⅱa had more abrupt curvature than mesiobuccal canals at the apical one third.

족관절 내과골절에서 피질골 나사못을 이용한 변형된 긴장대 고정술

최호림,도현우,김병흠,김규현,박종석,송준민 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적 : 전위된 족관절 내과골절에 대해 피질골 나사못을 이용한 변형된 긴장대 고정술을 이용한 치료결과를 분석해 보고자 하였다. 대상 및 방법 : 2001년 1월부터 2003년 1월까지 전위된 내과골절에 대해 변형된 긴장대 고정술을 시행 받고 최소 1년 이상 추시관찰 가능 하였던 24례를 대상으로 하였다. 남자 13례, 여자 11례로 평균 연령은 46세였다. 골절은 Lauge-Hansen 분류법을 사용하였으며, 결과는 Meyer와 Kumler의 평가 방법을 이용하였다. 결과 : 13례 (54%)에서 우수의 결과를 얻었고, 9례 (38%)에서 양호의 결과를 얻었으며, 운동범위의 제한이 발생한 1례에서 보통, 후외상성 관절염이 발생한 1례에서 불량의 결과를 얻었다. 결론 : 전위된 족관절 내과골절에서 피질골 나사못을 이용한 변형된 긴장대 고정술은 견고한 내고정과 조기 관절 운동이 가능한 효과적인 수술 방법이 될 수 있을 것으로 사료된다. Purpose : To evaluate the clinical results of modified tension band wire technique using cortical screw for treatment of displaced medial malleolar fractures of the ankle. Materials and Methods : From January 2001 to January 2003, 24 patients were treated by modified tension band wiring using cortical screw for medial malleolar fracture. The follow-up period was 12~35 months (average 18 months). There were 13 males and 11 females, and the mean age was 46 years. Fractures were classified by Lauge-Hansen's classification. The results were analyzed by Meyer and Kumler's criteria. Results : There were 13 cases (54%) of excellent, 9 cases (38%) of good, and one case of fair because of limitation of motion of the ankle joint and one case of poor which showed post-traumatic arthritis of the ankle. Conclusion : Modified tension band wire technique using cortical screw can be an effective operative method for the treatment of displaced medial malleolar fractures of the ankle.