Occlusal deviations in adolescents with idiopathic and congenital scoliosis

Zhang Hao,Ma Jingbo,Zhang Zhicheng,Feng Yafei,Cai Chuan,Wang Chao 대한치과교정학회 2022 대한치과교정학회지 Vol.52 No.3

Objective: This cross-sectional study aimed to investigate the characteristics of malocclusions in scoliotic patients through clinical examinations. Methods: Fifty-eight patients with idiopathic scoliosis (IS) and 48 patients with congenital scoliosis (CS) participated in the study. A randomly selected group of 152 orthopedically healthy children served as the control group. Standardized orthodontic and orthopedic examination protocols were used to record the occlusal patterns and type of scoliosis. Assessments were made by three experienced orthodontists and a spinal surgery team. The differences in the frequency distribution of occlusal patterns were evaluated by the chi-squared test. Results: In comparison with patients showing IS, patients with CS showed a higher incidence of Cobb angle ≥ 45° (p = 0.020) and included a higher proportion of patients receiving surgical treatments (p < 0.001). The distribution of the Angle Class II subgroup was significantly higher in the IS (p < 0.001) and CS (p = 0.031) groups than in the control group. In comparison with the healthy controls, the CS and IS groups showed significantly higher (p < 0.05) frequencies of asymmetric molar and asymmetric canine relationships, upper and lower middle line deviations, anterior deep overbite, unilateral posterior crossbite, and canted occlusal plane, with the frequencies being especially higher in CS patients and to a lesser extent in IS patients. Conclusions: Patients with scoliosis showed a high frequency of malocclusions, which were most obvious in patients with CS.

Identification of two putative phospholipid hydroperoxide glutathione peroxidase genes and the induction of three environmental stresses in Neoseiulus barkeri (Acari: Phytoseiidae)

Chuan Bei Tian,Guo Hao Zhang,Ya Ying Li,Huai Liu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

Phospholipid hydroperoxide glutathione peroxidase (PHGPX) is an antioxidant enzyme that plays a crucial role in metabolizing phospholipid hydroperoxides in membrane against oxidative damage. Here, two PHGPX genes fromthe predatory mite Neoseiulus barkeri were identified and characterized (NbPHGPX1 and NbPHGPX2). Alignment analysis of NbPHGPX1 and NbPHGPX2 showed a great deal of similarity with other known PHGPXs from databases. The well-conserved regions, NVASXCGXT, FPCNQFXXQEP, and IKWNFXKFLV, were surrounded by reactive cysteine, glutamine, and tryptophan residues, respectively. The results of quantitative polymerase chain reaction at different stages of the N. barkeri life cycle showed both NbPHGPX1 and NbPHGPX2 genes were highly expressed in male adults, and their levels of expression were determined upon challenge by different triggers. The data showed that the expression of NbPHGPX1 could be induced by low(4 °C) and high (42 °C) temperatures, UV-B, pyridaben and fenpropathrin,whereas NbPHGPX2 could be only up-regulated by high (42 °C) temperature and fenpropathrin. These results suggested that NbPHGPX1 and NbPHGPX2 genesmight participate in protection of the organism from the oxidative damage challenge by oxidative stresses.

Lactobacillus paracasei subsp. paracasei LC01 Positively Modulates Intestinal Microflora in Healthy Young Adults

Hao Zhang,Jing Sun,Xianting Liu,Chuan Hong,Yuanbo Zhu,Aiping Liu,Siqi Li,Huiyuan Guo,Fazheng Ren 한국미생물학회 2013 The journal of microbiology Vol.51 No.6

Lactobacillus paracasei subsp. paracasei LC01 (LC01) can tolerate intestinal stresses and has antioxidant activity. To evaluate the effect of the bacterium on human intestinal microflora,a randomized, double-blind, placebo-controlled human trial was carried out. Fifty-two healthy adult volunteers were randomized equally to two groups. One group consumed 12% (wt/vol) skimmed milk supplemented with 1010 CFU of LC01 each day for the 4-week treatment period,and then consumed placebo in the next treatment period,separated by a 2-week washout. The other group followed the reverse order. Group-specific real-time PCR and biochemical analyses was used to determine the intestinal bacterial composition of fecal samples collected at the end of every period, and the concentration of short-chain fatty acids and ammonia. A significant inhibition in fecal Escherichia coli and increase in Lactobacillus, Bifidobacterium, and Roseburia intestinalis were observed after consumption of LC01. Acetic acid and butyric acid were significantly higher in the probiotic stage and fecal ammonia was significantly lower. The results indicated a modulation effect of LC01 on the intestinal microflora of young adults, suggesting a beneficial effect on bowel health. LC01 may have potential value as a probiotic.

Synthesis, characterization and tribological performances of nano-CeO2/biodiesel carbon soot composites as a novel lubricant additive in polyalphaolefin

Chuan Li,Xinxin Wang,Qiangqiang Zhang,Xu Tan,Yefeng Liu,Honglin Li,Hao Liu,Enzhu Hu,Xianguo Hu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-

This study fabricated nano-CeO2/biodiesel carbon soot (BCS) composites using the raw material BCS,which is the byproduct of biodiesel combustion and cerium salt and used as a novel lubricant additiveto PAO6 for steel/steel contact. Oleylamine modified nano-CeO2/BCS composites comprise abundant lipophilicgroups, and thus nano-CeO2/BCS composites exhibit good dispersion in PAO6. The tribologicalresults showed that nano-CeO2/BCS composites significantly improved the lubricating performances ofPAO6. In case of 0.1 wt% nano-CeO2/BCS composites dispersed in PAO6, the friction coefficient and wearvolume were reduced by 17.3 and 35.2%, respectively. The improved tribological properties were attributedto the composites rolling and forming of a lubricating film on the friction surface and the presence ofBCS and CeO2 in composites exerting a synergistic lubricating effect, which simultaneously improved thetribological properties of PAO6. This discovery contributes to the conversion of BCS into valuables andpromotes the high value utilization of BCS as a lubricating additive.

Inhibitory Effects of Syk Transfection on Lung Cancer Cell Invasion

Peng, Chuan-Liang,Zhang, Ying,Sun, Qi-Feng,Zhao, Yun-Peng,Hao, Ying-Tao,Zhao, Xiao-Gang,Cong, Bo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro. Methods: A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined. Results: The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05). Conclusions: A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.

Scrotal Sparganosis Mimicking Scrotal Teratoma in an Infant

Yi-Ming Zhao,Hao-Chuan Zhang,Zhong-Rong Li,Hai-Yan Zhang 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.5

Bioconversion of conjugated linoleic acid by Lactobacillus plantarum CGMCC8198 supplemented with Acer truncatum bunge seeds oil

Dong-Ju Chen,Li-Hua Yan,Qian Li,Cai-jiao Zhang,Chuan-Ling Si,Zhong-Yuan Li,Ya-Jian Song,Hao Zhou,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.6

Conjugated linoleic acid (CLA) isomers, c9, t11- CLA and t10, c12-CLA, have been proved to exhibit excellent biomedical properties for potential use in anticancer applications and in reducing obesity. Acer truncatum Bunge (ATB), which is rich in unsaturated fatty acids, including oleic acid, linoleic acid, and nervonic acid, is a new resource for edible oil. In the present study, we developed a new method for producing two CLA isomers from ATB-seed oil by fermentation using Lactobacillus plantarum CGMCC8198 (LP8198), a novel probiotics strain. Polymerase chain reaction results showed that there was a conserved linoleate isomerase (LIase) gene in LP8198, and its transcription could be induced by ATBseed oil. Analyses by gas chromatography–mass spectrometry showed that the concentration of c9, t11-CLA and t10, c12-CLA in ATB-seed oil could be increased by about 9- and 2.25-fold, respectively, after being fermented by LP8198.

MicroRNA-21 Regulates the Invasion and Metastasis in Cholangiocarcinoma and May Be a Potential Biomarker for Cancer Prognosis

Huang, Qiang,Liu, Lei,Liu, Chen-Hai,You, Hao,Shao, Feng,Xie, Fang,Lin, Xian-Sheng,Hu, San-Yuan,Zhang, Chuan-Hai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Background: MicroRNAs are noncoding RNA molecules that posttranscriptionally regulate gene expression. The aim of this study was to determine the role of microRNA-21 in cholangiocarcinomas and its relationship to cholangiocarcinoma RBE cell capacity for invasion and metastasis. Methods: MicroRNA-21 expression was investigated in 41 cases of cholangiocarcinoma samples by in situ hybridization and real-time PCR. Influence on cholangiocarcinoma cell line invasion and metastasis was analyzed with microRNA-21 transfected cells. In addition, regulation of reversion-inducing-cysteine-rich protein with kazal motifs (RECK) by microRNA-21 was elucidated to identify mechanisms. Results: In situ hybridization and real-time quantitative PCR results for patients with lymph node metastasis or perineural invasion showed significantly high expression of microRNA-21 (P<0.05). There was a dramatic decrease in cholangiocarcinoma cell line invasion and metastasis ability after microRNA-21 knockdown (P<0.05). However, overexpression significantly increased invasion and metastasis (P<0.05). Real-time PCR and Western-blot analysis showed that microRNA-21 could potentially inhibit RECK expression in RBE cells. Survival analysis showed that patients with higher expression levels of microRNA-21 more often had a poor prognosis (P<0.05). Conclusions: MicroRNA-21 may play an important role in cholangiocarcinoma invasion and metastasis, suggesting that MicroRNA-21 should be further evaluated as a biomarker for predicting cholangiocarcinoma prognosis.

Magnetic Resonance Venography Findings of Obstructed Hepatic Veins and the Inferior Vena Cava in Patients with Budd-Chiari Syndrome

Ru-Xin Song,Shi-Feng Cai,Shuang Ma,Zhi-Ling Liu,Yong-Hao Gai,Chun-Qing Zhang,Guang-Chuan Wang 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.3

Objective: This study aimed to illustrate the magnetic resonance venography (MRV) manifestations of obstructed hepatic veins (HVs), the inferior vena cava (IVC), and accessory hepatic veins (AHVs) in patients with Budd-Chiari syndrome (BCS) and to evaluate the visualization capacity of MRV in the diagnosis of BCS. Materials and Methods: Fifty-two patients with chronic BCS were included in this study. All patients were examined via MRV performed with a 3T system following injections of gadolinium-diethylene triamine pentaacetic acid (Gd-DTPA) or Gdethoxibenzyl-DTPA. HV and IVC lesions were classified, and their characteristics were described. HV cord-like occlusions detected via MRV were compared using ultrasonography (US). Digital subtraction angiography (DSA) was performed as a contrast in the MRV detection of IVC lesions. The HVs draining collaterals, mainly AHVs, were carefully observed. HV lesions were classified as segmental stenosis, segmental occlusion, membranous stenosis, membranous occlusion, cord-like occlusion, or non-visualized. Except for patent IVCs, IVC lesions were classified as segmental occlusion, segmental stenosis, membranous occlusion, membranous stenosis, and hepatomegaly-induced stenosis. Results: All patients (52/52, 100%) showed HV lesions of different degrees. MRV was inferior to US in detecting cord-like occlusions (6 vs. 19, χ2 = 11.077, p < 0.001). Dilated AHVs, including 50 (50/52, 96.2%) caudate lobe veins and 37 (37/52, 71.2%) inferior HV and AHV lesions, were well-detected. There were no significant differences in detecting segmental lesions and thrombosis between MRV and DSA (χ2 = 0.000, p1 = 1.000, p2 = 1.000). The capacity of MRV to detect membranous lesions was inferior to that of DSA (7 vs. 15, χ2 = 6.125, p = 0.013). Conclusion: In patients with BCS, MRV can clearly display the lesions in HVs and the IVC, as well as in AHVs, and it has diagnostic and therapeutic value.

Influence of FeSe doping on superconducting properties of MgB2 by hybrid microwave method

Cheng Cheng,Zhenjie Feng,Qing Li,Xu Wang,Chuan Yu,Hao Chu,Ya Yang,Changqin Liu,Yiming Cao,Zhe Li,Jingzhe Chen,Chao Jing,Shixun Cao,Jincang Zhang 한국물리학회 2017 Current Applied Physics Vol.17 No.11

The effect of FeSe doping on the physical properties of MgB2 is studied. Bulk samples of the FeSe doped MgB2 with weight ratio x ðFeSe : MgB2Þ ¼ 0%; 3%; 7% and 10% were prepared by hybrid microwave method. It is proved that FeSe is not stable together with MgB2. Fe2þ enters into MgB2 lattice, some Mg2þ and Se2『 are combined into the new impurity compound MgSe. The superconducting transition temperature (Tc) slightly decreased with increasing doping content of FeSe from R-T and M-T curves, which results from the substitution of Mg2þ by Fe2þ in the MgB2 lattice. The Jc increase slightly with the FeSe doping content increasing from 3 wt % to 10 wt %, which results from the increasing MgSe impurity pinning centers.