Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Li Jing‐Jing,Jin Ming‐Hui,Wang Nian‐Meng,Yu Qi‐Tong,Shang Ze‐Yu,Xue Chao‐Bin 한국곤충학회 2020 Entomological Research Vol.50 No.10

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide‐resistance mechanism in DBM. The lab‐selected (Rh) and field‐collected (Rb) flubendiamide‐resistant lines of P. xylostella with resistance ratios of 1889.92‐fold and 1250.97‐fold, respectively, were used, as well as a lab‐reared flubendiamide‐susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the Rh group and 432 upregulated and 565 downregulated gene messengers in the Rb group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the Rh line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the Rb line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the Rh group and 221 upregulated and 155 downregulated proteins in the Rb group. Further analyses found that three CYP and 11 CYP proteins were over‐expressed in Rh and Rb lines, respectively. Four glutathione S‐transferase (GST) and four UGTs were over‐expressed in Rb line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

정식 시기와 적심 횟수가 화단국화의 생장과 개화에 미치는 영향

姚婧超, 정해준, 김동찬, 이진희, 권민훈, 서병기 배재대학교 자연과학연구소 2013 自然科學論文集 Vol.24 No.1

This study was conducted to investigate optimum planting date and pinching times on the growth and flowering of garden chrysanthemum(Dendranthema grandiflorum Ramat.). 3 lines of garden mums were planted on May 16, June 13 and July 11 respectively. As planting date was delayed, all the 3 lines' plant height and plant width was reduced. 09-19-49 line got the biggest plant height and width, followed by 09-11-37 line. And 08-166-02 line was the smallest. In different planting times, the experiment group of May 16 and June 13 got the biggest plant height, plant width and stem diameter. However, in the experiment group of May 16, most of the plants got flattened. The experiment group of June 13 got the best ornamental value, and the ornamental value was reduced in the experiment group of July 11 by the short vegetative growth. 09-19-49 line got the most flowers, while in different planting time, the experiment group of May 16 got the most flowers. 09-11-37 line got the biggest flower diameter. 09-11-37 line and 09-09-51 line were planted in the containers on the rooftop. Each groups were pinched by 1, 2 and 3 times on July 2, July 26 and Aug 16 respectively. There was no effect on plant height, plant width, and stem diameter of 09-09-51 line and 09-11-37 line between 1-time-pinching group and 2-time-pinching group. But 3-time-pinching group was reduced. There was no significance in flower diameter of these two lines but the number of flowers was reduced by more pinching times. Therefore, in this experiment, the groups which were planted on June 13 got the best ornamental value and the recommended planting time is in the middle of June. Furthermore, 2 times of pinching was recommended.

조경용 국화 품종 ‘Pansy Ball’과 육성 계통의 적정 식재 밀도

정해준 · 김동찬 · 姚婧超 · 서병기 배재대학교 자연과학연구소 2012 自然科學論文集 Vol.23 No.1

This research was carried out to investigate optimum planting density of landscape garden mum ‘Pansy Ball’ and 5 inbred lines. ‘Pansy Ball’ and 5 inbred lines of garden mums were planted at Pai Chai university campus on April 25, 2011. Plant density was controlled by 1 plant, 2 plants, 4 plants in one plot of 0.4m x 0.5m respectively. Average plant height in 1 plant in one plot was 46.2cm~71.7cm. And the average plant height in 2 plant in one plot was 45.9cm~64.8cm, while 4 plant in one plot was 38.9cm~53.2cm. Also, plant width of 05-18-01, 05-01-01, 09-76-03, and 09-02-02 inbred lines were over 50cm by 1 plant in one plot. However, as the plant height and plant density was high, most of the plants got flattened and the ornamental value was also reduced. We got the ‘Pansy Ball’ and 2 inbred lines 09-76-03, 09-02-02 for proper landscape ground cover plant.

Monitoring Analysis and Numerical Simulation of the Land Subsidence in Linear Engineering Areas

Chao Jia,Xiao Yang,Jing Wu,Pengpeng Ding,Chao Bian 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.7

Non-uniform land subsidence can cause subgrade deformation and threaten the safety of linear projects such as high-speed railways. The Lunan high-speed railway is taken as the research background. Combined with the hydrogeological and engineering geological conditions, the regional land subsidence is analyzed by the combined method of the differential interferometric synthetic aperture radar (D-InSAR) and small baseline subset interferometric synthetic aperture radar (SBAS-InSAR), which is verified by using precise leveling and GPS monitoring data. The accurate land subsidence analysis of large-scale linear engineering is realized. The distribution situation and change laws of land subsidence in linear engineering areas are revealed. Then, a three-dimensional coupled numerical model of land subsidence along a large-scale linear project is established by using the finite difference method. The spatial distribution characteristics of land subsidence in the linear engineering area are quantitatively analyzed and predicted. The main factors affecting land subsidence and the degree of land subsidence are discussed while keeping the pumping rate constant. The results show that: 1) The combined method of D-InSAR and SBAS-InSAR has high accuracy in the land subsidence monitoring of linear engineering. 2) There are five non-uniform settlement funnels along the Lunan high-speed railway. The groundwater over-extraction and coal mining are the main reasons for the land subsidence. 3) Realizing water source conversion and reduce groundwater exploitation are the main factors in linear engineering areas as soon as possible.

Diversity Distributions and the Anthocyanin Associations of Fungal Endophytes in Diferent Colored Grapevine Leaves

Jing‑Chao Chen,Man Yu,Fang Liu,Jin‑Zhuo Qu,Xiao‑Xia Pan,Han‑Bo Zhang,Ming‑Zhi Yang 한국식물학회 2020 Journal of Plant Biology Vol.63 No.2

Anthocyanins composed the major pigments which conferred sensory and chemical values to red grapes and wines. Multiple factors involved in grape anthocyanin biosynthesis have been greatly covered to date. However, the endophytes which closely associate with plants, their possible roles in plant coloration are still under clear. Our present research frstly investigated the diversity distributions of culturable fungal endophytes (CFE) in diferent colored grapevine leaves which harvested from same vineyard and cultivar, and then proposed method to analyze the possible efects of these fungal endophytes on anthocyanin concentrations of grape cells. In totally 532 endophytic fungal isolates, 19 OTUs belong to 13 genera were isolated from fve color diferent grapevine leaves. Obvious leaf color specifcity of CFE communities were observed distribution in diferent colored grapevine leaves in this experiment. Assessing the infuences of those isolated CFEs on anthocyanin concentrations of a teinturier grape pulp cells via dual culture system revealed that anthocyanin promotion fungal strains were more possibly isolated from red and purple colored grape leaves. And elite CFE strains such as DQ53 and DQ55, both belong to the fungal genus Epicoccum, which conferred signifcant promotion efects on grape cellular anthocyanin contents were selected out. And the transcriptions of anthocyanin biosynthesis association genes MYBA1, UFGT and F3′5′H in grape cells, were signifcantly infuenced by these endophytic fungi, furtherly implicated the involvements of these genes in fungal endophytes-mediated grape cell anthocyanin promotion process. Additionally, endophytic fungi triggered anthocyanin promotion in grape cells seem independently to that of the light mediated anthocyanin synthesis pathway. This work demonstrated that beside other environmental factors, endophytes may also involve in grape anthocyanin metabolisms. And the study provided methods and clues to explore fungal endophytes in colored grape leaves which possibly applied in grape pigmentation processing.

통계분석을 이용한 CCPM 기법에서의 버퍼 산정방법

유정초(Jing-Chao Liu),황보택근(Taeg-Keun Whangbo) 한국콘텐츠학회 2012 한국콘텐츠학회논문지 Vol.12 No.8

CCPM 기법에서는 프로젝트 버퍼의 크기를 결정하기 위해 일반적으로 자르고 붙이는 방법과 루트-제곱하는 방법을 사용한다. 하지만 이러한 방법은 프로젝트의 특성을 고려하지 않고 고정된 공식을 통해서만 계산하기 때문에 버퍼의 크기가 너무 커지거나 작아지는 경우가 자주 발생했다. 본 논문에서는 위의 문제점을 해결하기 위해 이전 작업의 결과를 통계 분석하여 각 작업자에 대한 각 임무의 종류에 따른 작업의 특징을 파악하고 이를 CCPM 기법에 활용하여 해당 특징을 기준으로 버퍼 크기를 산정하는 새 방법을 제시하였다. 몬테카를로 시뮬레이션 환경에서 임무의 수, 임무의 어려운 정도 등의 요소를 반영하여 나온 결과를 비교분석 해서 본 논문에서 제안한 방법이 기존의 방법에 비해 임무 수와 상관없이 안정된 완공확률을 유지할 수 있음을 확인하였다. 또한 특정 작업자가 일찍 완공할 수 있는 임무들의 경우 제안한 방법은 기존 방법보다 버퍼의 크기를 더 단축하는 것을 확인하였다. In CCPM Technique, as the buffer size calculation method, the Cut and Paste(C&P) method and the Root Square Error (RSE) method for all tasks carried out the same treatment, without considering the actual situation and characteristics of the task, the lack of reasonable judgment, is too simple and hasty. In this paper, taking into account the limitations of existing methods, a new method of buffer sizing method based on statistical analysis was introduced. It makes statistical analysis for the relationship between each worker and a variety of tasks, and use the information to predict the next task time. In order to verify the effectiveness of the new method, according to different task difficulty and the number of tasks set up the project. Use C&P, RSE method and new methods to predict the time of the project. Through Monte Carlo Simulation to simulate the project time, a comparison of three methods of performance. The results show that the new method can achieve the managers expect the probability of completion, and for those tasks can be completed ahead of schedule, the new method can save project time.

Construction of a Protein-Protein Interaction Network for Chronic Myelocytic Leukemia and Pathway Prediction of Molecular Complexes

Zhou, Chao,Teng, Wen-Jing,Yang, Jing,Hu, Zhen-Bo,Wang, Cong-Cong,Qin, Bao-Ning,Lv, Qing-Liang,Liu, Ze-Wang,Sun, Chang-Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Background: Chronic myelocytic leukemia is a disease that threatens both adults and children. Great progress has been achieved in treatment but protein-protein interaction networks underlining chronic myelocytic leukemia are less known. Objective: To develop a protein-protein interaction network for chronic myelocytic leukemia based on gene expression and to predict biological pathways underlying molecular complexes in the network. Materials and Methods: Genes involved in chronic myelocytic leukemia were selected from OMIM database. Literature mining was performed by Agilent Literature Search plugin and a protein-protein interaction network of chronic myelocytic leukemia was established by Cytoscape. The molecular complexes in the network were detected by Clusterviz plugin and pathway enrichment of molecular complexes were performed by DAVID online. Results and Discussion: There are seventy-nine chronic myelocytic leukemia genes in the Mendelian Inheritance In Man Database. The protein-protein interaction network of chronic myelocytic leukemia contained 638 nodes, 1830 edges and perhaps 5 molecular complexes. Among them, complex 1 is involved in pathways that are related to cytokine secretion, cytokine-receptor binding, cytokine receptor signaling, while complex 3 is related to biological behavior of tumors which can provide the bioinformatic foundation for further understanding the mechanisms of chronic myelocytic leukemia.

중국 고등학교 교사의 스마트교육에 대한 관심도 및 실행도 분석

쑹차오징(Chao Jing Song),배가나(Ga Na Bae),장재혁(Chea Huck Chang),황석현(Seok Hyun Hwang),김석우(Suk Woo Kim) 서울대학교 교육종합연구원 2022 The SNU Journal of Education Research Vol.31 No.1

본 연구의 목적은 중국 고등학교 교사의 스마트교육에 대한 관심도 및 실행도를 살펴보기 위해 실시되었다. 이를 위해 2021년 6월부터 7월까지 중국 산둥성 웨이팡시 고등학교 교사 281명을 대상으로 설문조사를 실시하였으며, 최종 239부를 기술통계, 상관분석, 다변량 분산분석(MANOVA)등을 실시하였다. 그 결과, 첫째, 중국 고등학교 교사의 스마트교육에 대한 관심도는 실행도 보다 높았다. 둘째, 설립형태별 분석 결과, 공립고등학교 교사들이 사립고등학교 교사에 비해 관심도 및 실행도 모두 높게 나타났다. 셋째, 최종학력별 분석 결과, 대학교 졸업인 교사보다 석․박사학위를 마친 교사들의 관심도가 더 높게 나타났다. 넷째, 개인배경별 관심도 및 실행도의 차이는 각각 다르게 나타났다. 이러한 결과는 2010년대부터 중국 정부 및 교육부에서 실시한 스마트교육을 다시 점검하고, 획일적인 스마트교육 교원연수를 벗어나 개인적 배경요인과 지역적인 상황 등을 고려한 다차원적이고 세밀한 스마트교육 교원연수로 나아가기 위한 기초자료로써 도움이 될 것이다. The purpose of this study was to examine the interest and implementation of Chinese high school teachers in smart education. To this end, a survey was conducted on 281 teachers at Weifang High School in Shandong Province, China from June to July 2021, and the final 239 copies were conducted with descriptive statistics, correlation analysis, and MANOVA. As a result, first, Chinese high school teachers interest in smart education was higher in implementation. Second, as a result of analysis by establishment type, public high school teachers showed higher interest and implementation than private high school teachers. Third, as a result of analysis by final educational background, teachers who completed master s and doctorate degrees were more interested than teachers who graduated from university. Fourth, the differences in interest and performance by individual background were different. These results will be helpful as basic data to re-check smart education conducted by the Chinese government and the Ministry of Education since the 2010s and move beyond uniform smart education teacher training to multi-dimensional and detailed smart education teacher training considering personal background factors and local situations.

Design and Evaluation of a Three-axis Force/Torque Sensor for Humanoid Robot Balance Control

Chao Yuan,Rui-Jun Yan,Jing Wu,Sang Ho Kim,Kyoo-Sik Shin,Chang-Soo Han 제어로봇시스템학회 2014 제어로봇시스템학회 국제학술대회 논문집 Vol.2014 No.10

For decades, popular culture has been enthralled with the possibility of robots that act and look like human. Walking, as one of the most important features of human, needs to be well implemented by humanoid robots. In order to perform stable robot gait, it is necessary to control the position of the Zero Moment Point (ZMP) of the robot. To calculate the ZMP, forces and moments under robot feet are important information to use. Thus, in this paper, we describe the development of a three-axis force/torque sensor to measure forces under robot feet in walking movement. The key point of this sensor is that it is very thin and light so as to not disturb the robots’ gait. It also has sufficient stiffness to support heavy load. The simple structure and low cost of this sensor are also attractive points. Finite element analysis results of the sensor revealed that it is adequate to be used under robot feet.

Research on Fracture of Aluminum Foil in Microscale Laser Peen Forming

Chao Zheng,Sheng Sun,Jing Liu,Zhong Ji 한국소성가공학회 2010 기타자료 Vol.2010 No.6

A novel numerical method for dynamic fracture in microscale laser peen forming (μLPF) of aluminum foils was presented and the role of the die diameter on fracture behavior at the ultra high strain rate was investigated via both experimental and numerical methods. μLPF is a process in which the plastic deformation is generated through laserinduced shock wave and compressive residual stresses can be imparted to improve the fatigue life of micro parts. During μLPF, the pressure exerted on the target is higher than 1 GPa and the strain rate is greater than 10? s<SUP>-1</SUP>, so the mechanical behavior of materials in this dynamic process is very different from that under static or quasi-static conditions. In the present study, the finite element method with grain and grain boundary elements was used to analyze the μLPF process of aluminum foils with a thickness of 60 μm. The onset and propagation of crack were simulated in this way that the specified nodes were tied together until the equivalent plastic strain exceeded a certain value. Under a given value of plastic strain, the influence of die diameters of 0.6, 1.0, and 1.6 mm on the fracture mode of the material was predicted. A series of experiments were carried out to verify the numerical model. The geometrical morphologies of fracture regions were observed via optical microscope and scan electron microscope. In results from both experiments and simulations, the size of the die diameter affects the location of the fracture: (I) Fracture appeared at the entrance of the die for die diameters of 0.6 and 1.0 mm. (II) Fracture occurred near the centre of the formed dome for 1.6 mm die diameter. The generation mechanism of two fracture modes was explained. This work provides a preliminary insight into the fracture behavior of materials under the ultra high strain rate and lays the ground work for more in-depth simulations in the future study.