Effect of Chromatin Changes in the Germinal Vesicle on the Development of Porcine Embryos In Vitro

Min‐Gu Lee,Rong‐xun Han,Jin‐Yu zhang,Yun‐Fei Diao,Reza K. Oqani,Dong‐Il Jin 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

In all the studies of mammalian species, chromatin in the germinal vesicle (GV) is initially decondensed with the nucleolus not surrounded by heterochromatin (the NSN configurations). During oocyte growth, the GV chromatin condenses into perinucleolar rings (the SN configurations) or other corresponding configurations with or without the perinucleolar rings, depending on species. During oocyte maturation, the GV chromatin is synchronized in a less condensed state before germinal vesicle breakdown (GVBD) in species that has been minutely studied. As not all the species show the SN configuration and gene transcription always stops at the late stage of oocyte growth, it is suggested that a thorough condensation of GV chromatin is essential for transcriptional repression. Because the GV chromatin status is highly correlated with oocyte competence, oocytes must end the NSN configuration before they gain the full meiotic competence and they must take on the SN/corresponding configurations and stop gene transcription before they acquire the competence for early embryonic development. In this study, we firstly investigated whether the layer of cumulus cells and size of oocytes could determine chromatin configurations in porcine oocytes. Using Hoechst3342 staining, the GV nucleolus and chromatin of porcine oocytes was classified into SN and NSN configurations. Next, we examined the changes in GV chromatin configurations during growth and maturation of porcine oocytes. In addition, the maturation and parthenogenetic development abilities in vitro were significant different between the SN and NSN configurations oocytes. These results indicated that chromatin changes in GV oocytes affect the development potential of parthenogenetic embryos.

Effect of sous-vide cooking conditions on the physicochemical, microbiological and microstructural properties of duck breast meat

Dong-Min Shin,Jong Hyeok Yune,Dong-Hyun Kim,Sung Gu Han Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.10

Objective: Sous-vide cooking offers several advantages for poultry meat, including enhanced tenderness, reduced cooking loss, and improved product yield. However, in duck meat, there are challenges associated with using the sous-vide method. The prolonged cooking time at low temperatures can lead to unstable microbial and oxidative stabilities. Thus, we aimed to assess how varying sous-vide cooking temperatures and durations affect the physicochemical and microbial characteristics of duck breast meat, with the goal of identifying an optimal cooking condition. Methods: Duck breast meat (Anas platyrhynchos) aged 42 days and with an average weight of 1,400±50 g, underwent cooking under various conditions (ranging from 50℃ to 80℃) for either 60 or 180 min. Then, physicochemical, microbial, and microstructural properties of the cooked duck breast meat were assessed. Results: Different cooking conditions affected the quality attributes of the meat. The cooking loss, lightness, yellowness, Hue angle, whiteness, and thiobarbituric acid reactive substance (TBARS) values of the duck breast meat increased with the increase in cooking temperature and time. In contrast, the redness and chroma values decreased with the increase in cooking temperature and time. Cooking of samples higher than 60℃ increased the volatile basic nitrogen contents and TBARS. Microbial analysis revealed the presence of Escherichia coli and Coliform only in the samples cooked at 50℃ and raw meat. Cooking at lower temperature and shorter time increased the tenderness of the meat. Microstructure analysis showed that the contraction of myofibrils and meat density increased upon increasing the cooking temperature and time. Conclusion: Our data indicate that the optimal sous-vide method for duck breast meat was cooking at 60℃ for 60 min. This temperature and time conditions showed good texture properties and microbial stability, and low level of TBARS of the duck breast meat.

공동주택 장기수선계획 시스템 개발을 위한 분석ㆍ설계단계에 관한 기초적 연구

서민구,정영한,박태근 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

Extension of building life cycle and reduction of maintenance and management relevant expenses are the top issues in the current construction market. The fields of maintenance and management are newly highlighted for the limited land and domestic economic crisis known as the 2nd IMF. We studied on the long-term maintenance plan of apartment buildings in order to analyze the defective management system of existing apartment building and secure the development-oriented management system in this study. Purposes of this study are to prevent the unwanted loss of manpower and capital resulted from the careless storage of repair and management records, extend the building life cycle and resolve the conflict caused by the uncomfortable relation with the occupants. This study is focused on presenting the potential to develop the process required for the basic analysis and development of system as the beginning stage in the process of developing the long-term maintenance plan.

친환경농업 시범마을에 대한 환경영향평가

이남종,고병구,노기안,한민수,김민경,곽한강,박문희 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

1999년부터 2001년까지 3년간 친환경농업 시범마을에 적용되고 있는 INM (Integrated Nutrient Management) and IPM (Integrated Pest Management) 실천에 따른 추진효과를 분석하기 위하여 시범마을로 지정된 옥천과 양평지역의 농가 포장에서 시비량, 양분유실 및 생태계 변화 등을 조사 분석하였다. 3요소 시비량은 토양검정시비로 인하여 농가관행시비와 비교하여 28.6~39.4% 절감할 수 있었으며, 쌀 수량도 시비량이 비교적 적은 토양검정 시비구에서 3~10% 증수하였다. 영농기간동안 토양 침투수중 NO₃-N 농도는 농가관행 시비구 2.9 ㎎/L에 비하여 토양검정 시비구는 1.5 ㎎/L로 감소하였으며, 시범마을 논으로 유입되는 관개수의 COD, NH₄-N, NO₃-N 농도는 각각 15.0, 0.67, 1.39 ㎎/L, 시범마을 논에서 배출된 용수는 각각 12.4, 0.29, 2.42 ㎎g/L로 감소되는 경향이었다. 처리별 수서곤충, 환형동물, 패류 및 감각류 등의 개체수는 농가관행구 100에 대하여 토양검정 시비구 293, 토양검정과 개량제 혼용구 276, 무비구 90으로 토양검정 시비구에서 많았으며 수서 무척추 동물은 관행농업을 수행하고 있는 지역을 100으로 볼 때 친환경농업마을은 242와 443으로 INM과 IPM을 실천하는 시범마을에서 생물종이 다양하고 그 개체수가 많은 것으로 나타났다. 친환경농업 시범마을 조성 후 작물 추천시비량 준수와 적기 예찰을 통하여 필요이상 사용되는 비료와 농약을 절감하여 생태계의 개선과 토양이 건전화된 것으로 평가되었다. In order to preserve the soundness of ago-ecosystem and products safety in agriculture, this study was evaluated environmental impact in relation to the INM (Integrated Nutrient Management) and IPM (Integrated Pest Management) at paddy fields at Ok-Chun and Yang-Pyung region. By introduction of INM with the application of BB(bulk-blanded) fertilizer based on soil analysis and EM, the application rate of fertilizer was reduced to about 28.6-39.4% and the yield of brown rice was increased to about 3-10% compared to conventional practices. The concentrations of COD, NH4-N, and NO3-N in irrigation water flowed to the environmental-friendly agriculture practices were 15.0, 0.67, and 1.39 ㎎/L, respectively. The concentrations of COD, NH₄-N, and NO₃-N from paddy fields in drainage water were 12.4, 0.29, and 2.42 ㎎/L, respectively. The total number of the freshwater invertebrates was higher in field treated with fektilhtion by prescription with soil testing. Also, the population density of aquatic insects was higher than the other fields at both demonstration villages. In conclusion, it was possible to reduce the amount of fertilizer and agricultural chemicals application, and increase the yield of rice by application of the environmental-friendly agriculture practices.

Flutamide가 수컷 생쥐의 유해감각예민도에 미치는 영향

장세헌,김명정,박제민,양구범,한병득 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

Objectives : Previous findings on nociception modulatory effect of testosterone were controversial. In this study, androgen receptor antagonist flutamide was used to reveal testosterone effect on baseline nociceptive sensitivity and stress-induced antinociception in male mice. Methods : Experiments consist 3 Parts: 1) tail flick latency(TFL, sec, 52±1℃) was measured before and 30 minutes after intraperitoneal injection of flutamide 150, 100, 50mg/kg or vehicle(N=15 for each group); 2) TFL was measured before and after serial injection of LHRH antagonist antide(1mg/kg, ip), and 30 minutes after flutamide(100mg/kg, ip) or vehicle; 3) With pretreatment of flutamide(100mg/kg, ip) or vehicle(N=15 for each group), TFL was measured before and after 5 minutes of forced-swim in cold water(15±1℃). Results : 1.TFL was significantly elongated(hypoalgesic) in the flutamide 100 and 150mg/kg groups. TFL of flutamide 150mg/kg group was significantly longer than 50mg/kg group. 2.Though pretreatment with antide itself did not make any change in TFL, it blocked antinociceptive effect of flutamide. TFL was elongated by serial injections in both flutamide and control group. 3.TFL was elongated by FS in both flutamide and control group. FS-induced elongation of TFL was significantly more prominent in flutamide group. Conclusions : From these results, it is suggested flutamide has antinociceptive effect on baseline TFL and potentiates FS- induced antinociception.

신주위 공간 및 요근을 침범한 췌장성 가성낭포 1례 : 진단및 경피적 배액술 Diagnosis and Percutaneous Drainage

이현경,오연희,이원재,한동선,오민구,이성우,박수성 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

Pancretic pseudocyst is a well-known complication of pancreatitis and may occur in unusual locations. Involvement of the left perirenal space and psoas muscle is rare. Radiologic findings and management of a surgically proven case of pancreatic pseudocyst extending to left perirenal space and psoas muscle is described.

Barrier protection via Toll-like receptor 2 signaling in porcine intestinal epithelial cells damaged by deoxynivalnol

Gu, Min Jeong,Song, Sun Kwang,Lee, In Kyu,Ko, Seongyeol,Han, Seung Eun,Bae, Suhan,Ji, Sang Yun,Park, Byung-Chul,Song, Ki-Duk,Lee, Hak-Kyo,Han, Seung Hyun,Yun, Cheol-Heui BioMed Central 2016 VETERINARY RESEARCH Vol.47 No.-

<P>Intestinal barrier is the first line of defense inside the body and comprises intercellular tight junction (TJ) proteins that regulate paracellular permeability. Deoxynivalenol (DON), a fungal metabolite often found in the contaminated food of domestic animals, is known to impair intestinal barrier function and may be involved in intestinal inflammation. Unlike in humans and mice, the importance of Toll-like receptor (TLR) 2 expressed in porcine intestinal epithelial cells is largely unclear. Therefore, the aim of the present study was to investigate whether TLR2 stimulation enhances intestinal barrier function and protects against DON exposure. We found that the cells treated with TLR2 ligands decreased the epithelial barrier permeability and enhanced TJ protein expression in intestinal porcine epithelial cells (IPEC-J2). In addition, pretreatment with TLR2 ligand, including Pam3CSK4 (PCSK) and lipoteichoic acid from <I>Bacillus subtilis</I>, prevented DON-induced barrier dysfunction by increasing the expression of TJ proteins via the PI3K-Akt-dependent pathway. It is likely that the DON-disrupted intestinal barrier caused biological changes of immune cells in the lamina propria. Thus, we conducted co-culture of differentiated IPEC-J2 cells in the upper well together with peripheral blood mononuclear cells in the bottom well and found that apical TLR2 stimulation of IPEC-J2 cells could alleviate the reduction in cell survival and proliferation of immune cells. Conclusively, TLR2 signaling on intestinal epithelial cells may enhance intestinal barrier function and prevent DON-induced barrier dysfunction of epithelial cells.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s13567-016-0309-1) contains supplementary material, which is available to authorized users.</P>

Anti-inflammatory effects of the fruits of Paulownia tomentosa on ovalbumin-induced allergic asthma

Min-Gu Kim,Jae-Won Lee,Hyung Won Ryu,Jae-Hong Min,Seong-Man Kim,Daseul Hwang,Sei-Ryang Oh,Sang-Bae Han,Kyung-Seop Ahn 한국실험동물학회 2020 한국실험동물학회 학술발표대회 논문집 Vol.2020 No.7

Draping simulation of carbon/epoxy plain weave fabrics with non-orthogonal constitutive model and material behavior analysis of the cured structure

Han, Min-Gu,Chang, Seung-Hwan Elsevier 2018 Composites. Part A, Applied science and manufactur Vol.110 No.-

<P><B>Abstract</B></P> <P>The aim of this study is to estimate the mechanical behavior including material failure of cured draped structures. Based on the basic tests for representative mechanical properties of woven fabric prepregs, non-orthogonal constitutive VUMAT code for draping simulation was developed. To calibrate the simulation code, experimental validation was carried out. The local shear deformation of the plain weave fabric draped onto the hemispherical structure was simulated, and the results showed that the simulation successfully followed the actual draping behavior with very low level of errors in the deformation of the prepregs and it predicted the shear angles in local area of the draped structures accurately. The deformation behavior with material failure of the cured hemisphere under compression was estimated with consideration of the local property changes. By using the above analysis technique, the initial mechanical behavior and the failure mode of the hemisphere were accurately estimated using the simulation technique.</P>

Hydrolyzed fumonisin B₁ induces less inflammatory responses than fumonisin B₁ in the co-culture model of porcine intestinal epithelial and immune cells

Gu, Min Jeong,Han, Seung Eun,Hwang, Kyoryen,Mayer, Elisabeth,Reisinger, Nicole,Schatzmayr, Dian,Park, Byung-Chul,Han, Seung Hyun,Yun, Cheol-Heui Elsevier/North-Holland 2019 Toxicology letters Vol.305 No.-

<P><B>Abstract</B></P> <P>Fumonisin B<SUB>1</SUB> (FB<SUB>1</SUB>), mainly produced by <I>Fusarium verticillioides</I> and <I>Fusarium proliferatum</I>, can be converted to the less toxic metabolite hydrolyzed FB<SUB>1</SUB> (HFB<SUB>1</SUB>) by enzymatic degradation. The application of an FB<SUB>1</SUB>degrading enzyme as a feed additive is a strategy to reduce fumonisin exposure of animals. However, the difference between the effect of FB<SUB>1</SUB> and HFB<SUB>1</SUB> on porcine intestinal immunity is poorly documented. We investigated the toxic effects of FB<SUB>1</SUB> and HFB<SUB>1</SUB> exposure on porcine gut barrier function and intestinal immunity by using a co-culture model of intestinal porcine epithelial cells (IPEC-J2) and porcine peripheral blood mononuclear cells (PBMCs). First, we confirmed that <I>Fusarium</I> mycotoxin (deoxynivalenol; DON), in the presence of an endotoxin (lipopolysaccharide: LPS), disrupted gut permeability of IPEC-J2 and induced inflammatory response in the co-culture system. FB<SUB>1</SUB> induced additional damage to gut barrier function and promoted pro-inflammatory responses in the presence of LPS and DON compared to only LPS/DON treatment. In the co-culture system, FB<SUB>1</SUB>/LPS/DON induced increased cell death of PBMCs and pro-inflammatory cytokines than LPS/DON treatment. In contrast, the application of HFB<SUB>1</SUB> resulted in reduced levels of chemokines and pro-inflammatory cytokines together with marginal immune cell death compared to FB<SUB>1</SUB>/LPS/DON in the IPEC-J2/PBMC co-culture system. These findings suggest that FB<SUB>1</SUB> aggravates LPS/DON-induced intestinal inflammation, and HFB<SUB>1</SUB> showed less toxicity to immune response. Therefore, enzymatic degradation of FB<SUB>1</SUB> to HFB<SUB>1</SUB> could be an effective strategy to reduce intestinal inflammation in pigs.</P>