단지무우 改良을 爲한 實用形質의 選拔指標에 關한 硏究

金翰琳,吳翰俊 濟州大學校亞熱帶農業硏究所 1991 亞熱帶農業硏究 Vol.8 No.-

단지무우(Raphanus sativus L. var. hortensis Baker f. gigantissimus Makino)의 選拔을 效率的으로 遂行하기 위하여, 母系選拔法으로 2回 選拔한 단지무우 23系統으로 試驗을 實施하고, 이들에 대한 實用形質을 調査하여 遺傳率. 遺傳相關, 表現型相關, 環境相關 및 經路係數를 究明한 結果는 다음과 같다. 1. 系統間에 있어서 葉數· 葉柄의 길이· 根長· 根徑· 根形指數· 抽根長 및 根重은 高度의 有意性이 있고, 葉長· 乾葉重· 葉缺刻數 및 岐根數는 有意性이 없었다. 2. 遺傳率은 乾葉重· 根重· 葉數가 크고, 根形指數· 根長· 葉柄의 길이·抽根長· 根徑이 中間程度이며, 葉長· 岐根數· 葉缺刻數는 작았다. 3. 形質相互間의 遺傳相關·表現型相關과 環境相關의 크기에는 一定한 傾向이 없었고, 一般的으로 遺傳相關은 表現型相關보다 높았다. 遺傳相關에 있어서 根重은 葉數· 根徑 및 岐根數와 正의 相關關係가 높아서 이들 形質이 增加할수록 根重도 增大되었다. 表現型相關에 있어서 根重은 乾葉重 및 根徑과 높은 正의 相關關係가 높아서 이들 形質이 增加할수록 根重도 增大되었다. 表現型相關에 있어서 根重은 乾葉重 및 根徑과 높은 正의 相關關係가 있고, 環境相關에 있어서는 根重과 乾葉重 및 抽根長과 正의 相關關係가 높았다. 4. 根長 및 岐根數가 根重에 直接미치는 效果가 크며, 根重과 相關度가 높은 根徑에서는 岐根數에 의한 間接效果가, 그리고, 抽根長에서는 根長에 의한 間接效果가 컸었다. 以上의 結果에서 根徑, 岐根數 및 葉數가 根重에 크게 영향을 주어, 단지무우의 收量을 위한選拔에 이들이 指標形質로 思料되며, 根重의 遺傳率도 크므로 收量에 대한 選拔의 效果가 기대된다. This study was conducted to estimate genotypic, phenotypic and environmental correlations, heritabilities and path coefficients of agronomic characters using 23 lines of Danji radish (Raphanus sativus L. var. hortensis Baker f. gigantissiumus Makino) which were selected two times by maternal selection. The results obtained ware summarized as follow ; 1. The number of leaves per plant, the petiole length, the root length, the diameter of root, the root shape index, the root part above ground and the root weight had significant differences among the lines, but the leaf length, the weight of dry leaves, the number of parted leaflets and the number of branched root had not. 2. The heritability estimates for the weight of dry leaves, the root weight and the number of leaves per plant were high, those of the root shape index, the root length, the petiole length, the root part above ground and the diameter of root were medium, and those of the leaf length, the number of branched root and the number of parted leaflets were small. 3. The genotypic, the phenotypic and the environmental correlation coefficients between the characters had no definite tendency, that is, the high estimates in the genotypic correlations were not always high in the phenotypic or in the environmental. The genotypic correlations were generally higher than the phenotypic. In the genotypic correlation, the root weight had highly positive correlations with the number of leaves per plant, the diameter of root and the number of branched root, i.e. the root weight increased with increasing these characters. In the phenotypic correlation, the root weight highly related with the weight of dry leaves and the diameter of root. The environmental correlations between the root weight and the weight of dry leaves or the root part above ground were highly positive. 4. The directs effect of the root length and the number of branched root on the root weight were high. The indirect effects of the root part above ground via the root length, that of the number of branched root via the diameter of root which had high correlations with the root weight were high. As the result above-mentioned, it was discovered that the diameter of root, the number of branched root and the number of leaves per plant had large influences upon the root weight, and it was thought that the selection of Danji radish for the yield would be effective as the heritability estimates of the root weight was high.

SD Rats를 이용 1-hexene의 흡입독성 연구

김현영,임철홍,정용현,이권섭,이성배,이준연,한정희,전윤석,이용묵 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4

The purpose of this study was to investigate the acute (4 hours) and repeated-dose (6 hours a day, 5 days a week, 4 weeks) toxic effects of I -hexene on Sprague- Dawley (SD) rats which were treated by inhalation. The results were as follows; I. The median lethal concentration (LC_(50)) was estimated 52,694 ppm (confidence limit 95%; 49,494~55,447 ppm) in acute inhalation. Abnormal clinical signs related to the l-Hexene were not observed with the acute inhalation dose. Gross findings of necropsy revealed on evidence of specific toxicity related to the 1-hexene, II. By repeated inhalation exposure the body weight of male were more or less reduced by the dose of 2,500 ppm and 5,000 ppm compared with control group. However there were no significant variation hematology and blood biochemistry for the exposed rats compared with the control rats. Abnormal clinical signs and gross findings of necropsy related to the 1-hexene were not shown. In conclusion when we exposed I-hexene to SD rats for 4 weeks, 5 days per week, 6 hours per day, the Lowest observed effect level (LOEL) was over 2,500 ppm and Non observed effect level (NOEL) was below 500 ppm.

후두 및 하인두 편평세포암종에서 E-cadherin의 면역조직화학적 발현

도남용,나한조,이도용,허준,최지윤,이홍영,임성철 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2

Background and Objectives : The cell-cell adhesion molecule E-cadherin is necessary for the maintenance of the epithelial cellular structure. We were designed to confirm the significance of E-cadherin as a marker for differentiation and invasiveness of squamous cell carcinoma of the larynx and hypopharynx. Materials and Methods : Our study was investigated for the immunohistochemical expression of the epithelium-specific cell adhesion molecule E-cadherin in formalin fixed paraffin embedded tissue specimen of 32 cases of squamous cell carcinoma of larynx and hypopharynx. Results : The positive expression of E-cadherin was 56.3%(18 cases) in squamous cell carcinoma of the larynx and hypopharynx. Decreased E-cadherin expression was a stastistically significant correlation with a increased grade of lymph node stage and clinical stage. Reduced expression was seen in the large tumor size and poorly differentiated tumors, but these result was not statistically significant. Conclusion : The expression of E-cadherin may be related with progression of squamous cell carcinoma of the larynx and hypopharynx. But these correlation were not sufficient for the prognostic indicators in squamous cell carcinomas of the larynx and hypopharynx.

통풍 환자에서의 지질대사

이은우,강기서,강수용,이한준,김경환,임정일,박철경 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.2

Objective: To evaluate hyperlipidemia, a msjor tisk factor of coronary artery disease, frequently asscoiated with gout patients, we compared the mean lipid level of gout patient with that of non-gout male and accessed statistical significance for treatment of gout patient. Method: This study ws performed in the 266 male gout outpatients of the CAU medical center, who had serum lipid level test from Apr. 1996 to Aug. 2000. Serum uric acid, total cholesterol, triglyceride, HDL -cholesterol, and LDL-cholesterol level were measured after 12 hours of fasting. The patients with high lipid level were further evaluated into each type of the hyperlipidemia through electrophoresis study, and the test results were compared with that of the non-gout male adults(40∼60yr), and the statistical significance was examined. Results : The gout patients showed high levels of total cholesterol, triglyceride, and LDL-cholesterol. Especially they showed high level of triglyceride and LDL-cholesterol which suggested as the risk factors of atherosclerosis and coronary heart disease, but shoewed low level of HDL -cholesterol. But there were no definite relations between lipid level and other factors auch as age of patient, initial uric acid ;eve; and duration od gout. Conclusion: To evaluate the risk factors of atherosclerotic change of cadriac vascular system in gout patient, screening tests of serum lipid level of the patient seem mandatory.

두가지 혈류 유발방법에 의한 새로운 심폐소생술(이중 혈류 유발 심폐소생술)장치의 개발

황성오,김현,조준휘,오범진,임종천,최경훈,윤정한,이승환,김영식,이강현,이윤선 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: There have been many efforts to augment blood flow during cardiopulmonary resuscitation. These efforts have focused on maximizing the effect of cardiac pump or thoracic pump alone. However, considering that the heart is the biggest blood reservoir and increase of intrathoracic pressure can generate blood flow, simultaneous exploitation of both mechanisms may have synergistic effect. We hypothesized that simultaneous chest constriction in addition to sternal compression by standard CPR may have additive hemodynamic effects by preventing deformation of the chest and increase of intrathoracic pressure. Methods and results: we built a new mechanical device to perform compression and thoracic constriction simultaneously. The device consists of two main elements. Piston in the center is to depress the sternum. Strap is to constrict the thorax circumferentially. Strap is attached to both sides of the piston. When the piston is pushed down, it depresses the sternum and pulls on the thoracic strap. To determine strap width to produce optimal hemodynamic effect, we measured hemodynamic parameters with variable widths of strap in two dogs after induction of ventricular fibrillation. Result of the experiment showed that 10cm wide strap was determined to be most effective. We also determined optimal depth of compression to produce maximal hemodynamic effect with animal experiments using two dogs. Animal experiments showed that the highest aortic pressure could be generated when the stemum was depressed to 5 cm. Cardiopulmonary resusciation using a new device could generate higher systolic aortic pressure, coronary perfusion pressure and end-tidal carbon dioxide tension in comparison with standard cardiopulmonary resuscitation in a pilot animal study using two dogs. Conclusion: New cardiopulmonary resuscitation method using a mechanical device designed by us could perform sternal compression and simultaneous thoracic constriction, and generate better hemodynamic effects than standard cardiopulmonary resuscitation in pilot animal experiments.

Graphene oxide induces apoptotic cell death in endothelial cells by activating autophagy via calcium-dependent phosphorylation of c-Jun N-terminal kinases

Lim, Mi-Hee,Jeung, In Cheul,Jeong, Jinyoung,Yoon, Sung-Jin,Lee, Sang-Hyun,Park, Jongjin,Kang, Yu-Seon,Lee, Hansu,Park, Young-Jun,Lee, Hee Gu,Lee, Seon-Jin,Han, Baek Soo,Song, Nam Woong,Lee, Sang Chul Elsevier 2016 Acta Biomaterialia: structure-property-function re Vol.46 No.-

<P><B>Abstract</B></P> <P>Despite the rapid expansion of the biomedical applications of graphene oxide (GO), safety issues related to GO, particularly with regard to its effects on vascular endothelial cells (ECs), have been poorly evaluated. To explore possible GO-mediated vasculature cytotoxicity and determine lateral GO size relevance, we constructed four types of GO: micrometer-sized GO (MGO; 1089.9±135.3nm), submicrometer-sized GO (SGO; 390.2±51.4nm), nanometer-sized GO (NGO; 65.5±16.3nm), and graphene quantum dots (GQDs). All types but GQD showed a significant decrease in cellular viability in a dose-dependent manner. Notably, SGO or NGO, but not MGO, potently induced apoptosis while causing no detectable necrosis. Subsequently, SGO or NGO markedly induced autophagy through a process dependent on the c-Jun N-terminal kinase (JNK)-mediated phosphorylation of B-cell lymphoma 2 (Bcl-2), leading to the dissociation of Beclin-1 from the Beclin-1–Bcl-2 complex. Autophagy suppression attenuated the SGO- or NGO-induced apoptotic cell death of ECs, suggesting that SGO- or NGO-induced cytotoxicity is associated with autophagy. Moreover, SGO or NGO significantly induced increased intracellular calcium ion (Ca<SUP>2+</SUP>) levels. Intracellular Ca<SUP>2+</SUP> chelation with BAPTA-AM significantly attenuated microtubule-associated protein 1A/1B-light chain 3-II accumulation and JNK phosphorylation, resulting in reduced autophagy. Furthermore, we found that SGO or NGO induced Ca<SUP>2+</SUP> release from the endoplasmic reticulum through the PLC β3/IP<SUB>3</SUB>/IP<SUB>3</SUB>R signaling axis. These results elucidate the mechanism underlying the size-dependent cytotoxicity of GOs in the vasculature and may facilitate the development of a safer biomedical application of GOs.</P> <P><B>Statement of Significance</B></P> <P>Graphene oxide (GO) have received considerable attention with respect to their utilization in biomedical applications. However, GO-related safety issues concerning human vasculature are very limited. In this manuscript, we report for the first time the differential size-related biological effects of GOs on endothelial cells (ECs). Notably, Subnanometer- and nanometersized GOs induce apoptotic death in ECs via autophagy activation. We propose a molecular mechanism for the GO-induced autophagic cell death through the PLCβ3/IP3/Ca<SUP>2+</SUP>/JNK signaling axis. Our findings could be provide a better understanding of the GO sizedependent cytotoxicity in vasculature and facilitate the future development of safer biomedical applications of GOs.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Effects of the tacticities of poly(vinyl alcohol) on the structure and morphology of poly(vinyl alcohol) nanowebs prepared by electrospinning

Lim, Hyun Ju,Lee, Sung Jun,Bae, Han Jo,Noh, Seok Kyun,Lee, Yong Rok,Han, Sung Soo,Jeon, Han Yong,Park, Won Ho,Lyoo, Won Seok Wiley Subscription Services, Inc., A Wiley Company 2007 Journal of applied polymer science Vol.106 No.5

<P><B>Abstract</B></P><P>The effects of tacticities on the characteristics of poly(vinyl alcohol) (PVA) nanowebs prepared by an electrospinning technique were investigated. PVA webs composed of uniform nanofibers with syndiotactic dyad (s‐dyad) contents of 53.5 and 57.3% were successfully obtained with electrospinning. By changing processing parameters such as the initial polymer concentration, applied voltage, and tip‐to‐collector distance, we found suitable conditions for forming PVA webs with uniform nanofibers. PVAs of higher s‐dyad contents were prepared at a lower solution concentration and at a higher applied voltage because of the easy formation of syndiotactic PVA chain entanglements at a very low polymer concentration. The average diameter of the nanofibers in a PVA web with the higher s‐dyad content of 57.3% (ca. 240 nm) was thinner than that of the nanofibers in a PVA web with the lowers‐dyad content of 53.5% (ca. 270 nm). In addition, the crystallinity and thermal stability were greatly increased with an increase in the s‐dyad content. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007</P>

Heterogeneity in liver histopathology is associated with GSK-3β activity and mitochondrial dysfunction in end-stage diabetic rats on differential diets

( Jun-ho Lee ),( Soo-bong Choi ),( Dong-jun Sung ),( Mingli Jin ),( Ju-han Lee ),( Ji-young Mun ),( Tae-sook Hwang ),( Sang-don Han ),( Young-tae Ro ),( Sung-young Kim ),( Jueng-soo You ),( Inja Lim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2020 BMB Reports Vol.53 No.2

While liver histopathology is heterogeneous in diabetes, the underlying mechanisms remain unclear. We investigated whether glycemic variation resulting from differential diets can induce heterogeneity in diabetic liver and the underlying molecular mechanisms. We generated end-stage non-obese diabetic model rats by subtotal-pancreatectomy in male Sprague- Dawley rats and ad libitum diet for 7 weeks (n = 33). The rats were then divided into three groups, and fed a standard- or a low-protein diet (18 or 6 kcal%, respectively), for another 7 weeks: to maintain hyperglycemia, 11 rats were fed ad libitum (18AL group); to achieve euglycemia, 11 were calorierestricted (18R group), and 11 were both calorie- and proteinrestricted with the low-protein diet (6R group). Overnightfasted liver samples were collected after the differential diets together with sham-control (18S group), and histology and molecular changes were compared. Hyperglycemic-18AL showed glycogenic hepatopathy (GH) without steatosis, with the highest GSK-3β inactivation because of Akt activation during hyperglycemia; mitochondrial function was not impaired, compared to the 18S group. Euglycemic-18R showed neither GH nor steatosis, with intermediate GSK-3β activation and mitochondrial dysfunction. However, euglycemic-6R showed both GH and steatosis despite the highest GSK-3β activity and no molecular evidence of increased lipogenesis or decreased ApoB expression, where mitochondrial dysfunction was highest among the groups. In conclusion, heterogeneous liver histopathology developed in end-stage non-obese diabetic rats as the glycemic levels varied with differential diets, in which protein content in the diets as well as glycemic levels differentially influenced GSK-3β activity and mitochondrial function in insulin-deficient state. [BMB Reports 2020; 53(2): 100-105]

Aortocaval Fistula by Luetic Abdominal Aortic Aneurysm : Report of a case

Lim, Sang Hyun,Lee, Cheol Joo,Roh,Hwan-Kyu,Kim, Dae Jun,Han, Jung Sun,Shin, Joon Han,Kim, Han Soo 아주대학교 1996 아주의학 Vol.1 No.2

A 53-year-old male patient was operated on because of a spontaneous abdominal aortic aneurysm with aortocaval fistula. The fistula was successfully closed and bifurcated woven Dacron graft was used to replace the aneurysm. The patient had with characteristic signs and symptoms of aortic aneurysm with arteriovenous fistula. Proper diagnosis and prompt surgical repair of aortocaval fistula saved the patient. The use of rapid infusion system provided stable hemodynamics during the operation.

Effects of water temperature changes on oxygen consumption and hematological factors in olive flounder Paralichthys olivaceus

Lim, Han Kyu,Han, Hyon Sob,Hur, Jun Wook The Korean Society of Fisheries and Aquatic Scienc 2021 Fisheries and Aquatic Sciences Vol.24 No.2

Water temperature (WT) is a major environmental factor of metabolic rate in fish; it directly affects food intake, ammonia excretion, oxygen consumption (OC), growth, and survival. Thus, the purpose of this study was to investigate changes in the OC and the hematological response of olive flounder Paralichthys olivaceus because of WT changes. In Exp. I, WT was increased from 20℃ to 29℃ within 18 h and maintained at 29℃ for 96 h. Then, WT was decreased from 29℃ to 20℃ within 18 h and maintained at 20℃ for 24 h. In Exp. II, WT was decreased from 20℃ to 11℃ within 18 h and maintained at 11℃ for 96 h. Then, WT was increased from 11℃ to 20℃ within 18 h and maintained at 20℃ for 24 h. The Exp. III maintained that the Exp. I and II was consecutively. In Exp. I, the OC increased from 116.7 mg O₂ kg^-1 hr^-1 to 317.5 mg O₂ kg^-1 hr^-1 with increasing WT. In Exp. II, the OC decreased from 96.5 mg O₂ kg^-1 hr^-1 to 71.3 mg O₂ kg^-1 hr^-1 with decreasing WT. In Exp. III, OC tended to increase or decrease in inverse proportion to temperature. In Exp. I, cortisol, glucose, and aspartate aminotransferase (AST) values increased with increasing WT. In Exp. II, Cl^-, osmolality, AST, and alanine aminotransferase (ALT) values significantly changed during the experimental period: glucose values increased, whereas cortisol values decreased with decreasing WT. Exp. III was shown to be a more stressful environment to olive flounder than Exp. I and Exp. II. The results of our study will be useful for evaluating current aquaculture procedures of olive flounder and developing techniques to minimize stress in aquaculture farms.