건강식품 바이탈 및 비지놀의 抗疲勞 效果에 관한 연구

정경수,김혜경,김희수,김계양,곽영숙,김학범 충남대학교 약학대학 의약품개발연구소 1993 藥學論文集 Vol.9 No.-

As a result of extensive research efforts on the oriental medicinal herbs, some of their interesting biological activities have been elucidated. In the present study, antifatigue effects of two natural food supplements consisting of oriental herbs, Vital and Vigilor, were investigated using the ICR mice. The mice were fed with antibiotic-free normal mouse feed containing Vital or Vigilor for 14,28, or 31 days and then subjected to forced swim tests. In a test carried out in melting-ice water, Vital and Vigilor significantly increased the exhaustion times of the 28 day-fed mice by 26.9 % (p < 0.01) and 16.2% (p < 0.05), respectively. However, in the other test performed at 15±0.5℃ using 31 day-fed mice, Vigilor failed to increase the exhaustion time significantly (at p< 0.05), while Vital showed antifatigue effect by increasing the swimming time by 29.9% (p<0.01). Neither of Vital nor Vigilor exerted antifatigue effects in a swim test carried out on day 14 after the initiation of feeding. These results suggest that Vital and Vigilor exhert antifatigue effets when administered for a sufficient period of time by enhancing the health of the host animals.

제주도 토양에서 분리한 가시아메바의 riboprinting에 의한 동정

김현동,전자호,유학선,공현희 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.4

목적 : 가시아메바의 국내 분리주를 확보하고 국내 분포하는 가시아메바의 종류를 파악하며 그 분리주들의 병원성을 간접적으로 평가하기 위해 제주도의 토양에서 분리한 가시아메바 분리주를 riboprinting을 이용하여 종 동정을 하고자 한다. 재료 및 방법 : 제주도의 서로 다른 일곱 장소에서 무균 튜브에 흙을 담아와서 agar plate에서 배양한 후 서로 다른 형태적 특징을 보이는 포낭을 cloning하여 무균배양으로 키웠다. 각각의 핵 DNA를 분리하여 small subunit ribosomal RNA coding DNA 중 일부를 증폭하여 나온 PCR 산물을 8가지 제한효소로 소화시켜 그 RFLP양상을 16비교주와 비교한 후 Nei & Li의 공식에 대입한 후 UPGMA program로 유전적 거리를 구하였다. 결과 : 포낭의 형태는 KA/CS8주를 제외한 7주는 모두 Pussard 및 Pons의 분류에 따른 제 2군에 속하였고 KA/CS8주는 제 3군에 속하였다. 가시아메바 KA/CS1주와 KA/CS2주는 동일한 RELP양상을 나타내었으며 이들은 카스텔라니 가시아메바 Castellani주와 가장 유전적으로 가까왔고, KA/CS3주와 KA/CS6주는 A. triangularis SH621주와, KA/CS4주는 대식가시아메바 Jones주와, KA/CS5주는 대식가시아메바 P23주와. KA/CS7주는 카스텔라니 가시아메바 Neff주와 각각 유전적으로 가장 가까왔고, KA/CS8주는 비교주 중 어느 종과도 유전적으로 가깝지 않았다. 결론 : Acanthamoeba KA/CS1, KA/CS2, KA/CS3, KA/CS4, KA/CS6분리주들은 카스텔라니 가시아메바 complex에 속하였고, KA/CS5, KA/CS7분리주들은 대식가시아메바 complex에 속하였다. In order to collect and identify Acanthamoeba Korean strains. Acanthamoeba strains were isolated from soil of Cheju-do and were identified by comparison of the riboprints with the reference strains. Seven out of eight isolates from soil of Cheju-do belonged to the morphological group Ⅱ (Pussard and Pons, 1977) and the other one belonged to group Ⅲ by cyst morphology. The riboprints of seven isolates belonging to group Ⅱ were compared to those of Acanthamoeba reference strains (11 species 16 strains). KA/CS1, KA/CS2, KA/CS3, KA/CS4 and KA/CS6 strains belonged to A. castellanii complex. KA/CS1 and KA/CS2 strains showed identical riboprints to each other and were the most closely related to A. castellanii Castellani strain. KA/CS3 and KA/CS6 strains were the most closely related to A. triangularis SH621 strain, and KA/CS4 strain to A. polyphaga Jones strain. KA/CS5 and KA/CS7 strains belonged to A. polyphaga complex. KA/CS5 strain was the most closely related to A. polyphaga P23 strain and KA/CS7 strain to A. castellanii Neff strain. KA/CS8 strain belonging to group Ⅲ showed more than 20% estimated sequence divergence from the reference strains (5 species) and made a distinct division in a phenogram reconstructed based on the riboprints.

TKR와 TSR·TCR간 연계추진에 관한 복합운송업체의 인식분석

김경희,이학승 한국물류학회 2004 물류학회지 Vol.14 No.3

동북아지역을 관통하는 철도운송에 대한 관심이 2000년 6월 남북정상회담을 통한 9월 경의선 복원공사 착공으로 가시화되기 시작하였다. 이들 동북아지역은 러시아, 중국, 한반도, 그리고 일본 등과 서로 밀접한 관계를 맺고 있으며, 한반도는 이들 국가들의 한 가운데 위치하고 있으면서 동북아지역의 징검다리 역할을 하고 있다. 또한 동북아지역에 대한 관심이 고조되는 가운데, 이들을 연결하는 한반도종단철도(TKR)를 비롯한 대륙횡단철도인 중국횡단철도(TCR)과 시베리아횡단철도(TSR)에 대한 연구들이 활발히 진행되고 있는 실정이다. 따라서 본연구에서는 TSR과 TCR에 대한 최근 현황 및 문제점에 대해 살펴보고,TKR의 연계추진과정을 알아보고자 한다. 그리고 현재 수도권에 집중하여 영업망을 가진 복합운송업체들의 대상으로 설문조사를 통해 대륙철도운송에 대한 인식 파악과 향후 이들 업체들의 인식 개선 방안에 대해 논의하고자 한다. This paper wishes to analyze perception of forwarder about linkage-drive between Trans Korea Railway(TKR) and Trans Siberian Railway(TSR)-Trans China Railway(TCR) that is center of continent transport railroad. This study method and detailed contents are describing in paper Chapter 4 which see, and the study analysis used SPSS 10.0. The interest about railway through the Northeast Asia Area is appeared with Gyeong-wei Line restitution construction work at September through the South-North Summit-level Conference which hold in June, 2000. This Northeast Asia Area is bounded with a close relationship each other -Russia, China, Korea Peninsula, and Japan. And Korea Peninsula is located in center of these nations, so is doing the important duties in the Northeast Asia Area. Therefore This paper is analyzed the linkage-drive process of TKR after is examined the recent present statue and problem point about TSR-TCR. And I is discussed about perception improvement plan to the object of the forwarder which engaged now in Seoul. This analysis is examined with the question investigation. I could know that forwarders are not comprehend perfectly the concept about linkage-drive of continent cross-transport railway and TKR that doing now combined transportation.

제 2형 당뇨병 환자에서 혈중 Metalliproteinase-9의 농도

김상용,김희중,주유철,최동현,배학연 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2

Purpose : Diabetic nephropathy is a serious complication of diabetes and accounts for much of its morbidity and mortality. It is well known that the overt clinical phase of diabetic nephropathy is preceded for many years by the presence of microalbuminuria. Microalbuminuria is thought to be a marker of widespread vascular damage and may underlie the propensity toward severe extrarenal vascular disease in microalbuminuric patients. Metalloproteinase-9 has a broad substrate specificity for native collagens as well as proteoglycan and elastin. One study reported that an increased plasma metalloproteinase-9 concentration precedes the development of microalbuminuria in type 2 diabetic patients. Another study reported that a high glucose concentration cause a abnormality on the activity of metalloproteinase by the mesangeal cell. The purpose of the present study was to examine whether palsma metalloproteinase-9 concentrations are altered at various complications in type 2 diabetic patients. Methods : Sixty patients with type 2 diabetes and thirty healthy control were recruited for the present study. No patient had any malignancy or history of heart disease, liver disease, collagen disease. We recorded age, duration of diabetes, and blood pressure. After an overnight fast, blood was drawn from an antecubita1 vein of measurement of glucose, metalloproteinase-9, HbA1C, and serum creatinine. To measure plasma concentration of metalloproteinase-9, a one step sandwich enzyme immunoassay using two monoclonal antibodies was used. Patients were divided into two group according to the presence or abscence of microalbuminuria. The groups were then compared with regard to clinical and laboratory characteristics and serum concentration of metalloproteinase-9. Results : The mean serum levels of metalloproteinase-9 in diabetic patients (62.32±25.83ng/㎖) were significantly higher than in healthy controls(12.92±4.97ng/㎖) (p<01). The mean serum levels of metalloproteinase-9 in diabetic patients with microalbuminuria(81.16±41.43ng/㎖) were significantly higher than in those with normoalbuminuria(31.48±7.34ng/㎖) (p<01) and much more than in healthy control(12.92±4.97ng/㎖) (p<05). Furthermore, the severity of nephropathy were closely associated with the serum metalloproteinase-9 level and the serum metalloproteinase-9 level was significantly correlated with microalbuminuria. The mean serum level of metalloproteinase-9 in diabetic patients with retinopathy(61.74±39.07ng/㎖) was significantly higher than in those with no retinopathy(34.98±9.02ng/㎖) (p<05). But, in diabetic patients with normoalbuminuria, there is no difference between the groups with retinopathy versus no retinopathy. Conclusion : In summary, a sustained increase in serum metalloproteinase-9 in patients with type 2 diabetes is associated with a high risk for the development of microalbuminuria. The results of this study suggest that the serum rnetalloproteinase-9 was a useful and noninvasive marker for the development of diabetic nephropathy. It could be also used to predict the development of diabetic complications and to assess therapeutics or the prognosis of diabetes. The usefulness of this model will be clarified by a larger scale and serial study.

초등학교 여교사의 직무만족과 역할갈등 분석

김용주,이학주,정복희 경상대학교 2000 學生生活硏究所報 Vol.26 No.-

The purposes of this study were to examine the job satisfaction and role conflict of female elementary school teachers, and to analyze the relationship between those two factors. The following four research questions were raised. 1. What are the causal factors that brings about the job satisfaction of female elementary school teachers? 2. In regard to these causal factors, are there significant differences dependant upon such variables as teaching experience, the size of schools, and marital status? 3. What is the level of role conflict for married female elementary school teachers, and how significant are the differences according to the above variables? 4. What are the relationships between the job satisfaction factors and role conflicts of female elementary school teachers? For the purpose of this study, the researchers conducted questionnaire survey to the 300 female elementary school teachers in Kyong-nam Province. The study instrument is composed of 38 five-point Likert type questions. Major results of this research were as follows. First, the causal factors that bring about the job satisfaction of female elementary school teachers largely include school administration, a sense of recognition, promotions and raises, a physical environment, job duties, relationships with other teachers, meaningfulness of teaching, and working conditions. Second, the score on the job satisfaction of female elementary school teachers was above average(3.42 of 5.00). In regard to teaching experience, the respondents who have more than 20 years of experience had higer scores than the other groups of respondents. As for the size of schools, it was shown that schools with fewer than 23 classes had a relatively higher satisfaction rate than the other groups. Marital status had no significant differences. Third, the degree of the role conflict of married female elementary school teachers was low on the whole. Rather than the role conflict between home life and teaching duties as a teacher, the one as a housewife who has a job was greater. While teaching experience had no significant differences, the number of children had significant relations with female teachers role conflicts. Fourth, it was shown that the relationship between the causal factors of job satisfaction and role conflict of female elementary school teachers had no significant co-relationships.

침전성인자를 이용한 생물학적 고형물의 침전속도식과 고형물 플럭스 분석

김진우,최영균,이종훈,김희준,정태학 대한상하수도학회 2002 상하수도학회지 Vol.16 No.3

Equations on settling velocity of the activated sludge were developed by including sludge settleability parameters (SSP) and solids flux at a secondary settling tank was analyzed using the empirical equations, and an optimal solids loading rate was evaluated in this study. SVI, SSVI, DSVI and SSVI_3.5 were adopted as the SSP and a best fitting between observed and calculated zone settling velocity was obtained when SSVI_3.5 was used. Measured solids flux at the secondary settling tank was lower than that recommended by the national design criteria. Optimal solids loading rate and subsequent limiting solids flux can be derived if SSVI_3.5 is used instead of other SSP. Limiting solids flux calculated using SVI showed higher value than that calculated using SSVI_3.5 and the limiting solids flux could be better estimated when SSVI_3.5 was used because the calculated zone settling velocity using SSVI_3.5 was closer to the measured value.

체외형 자동 제세동기(AED)와 심전도계(ECG)를 위한 통신 프로토콜의 설계 및 사용자 인터페이스 구현

김남훈,권희중,권호열,나학록 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.A

In this paper, we studied Automated External Defibrillator and Electro- Cardiogram System. Proposed System, CU-EX1, is a PC-based AED/ECG system that manages the data acquired by Paramedic CU-ER1. The system controls the transfer of data from the Paramedic CU-ER1 and the storage of the data in a local host computer. Patient data could be entered as soon as the rescue data is stored in a local computer. The system also displays the data on a local computer and facilitates review of rescue events.

韓國産 穀物에서의 trichothecene mycotoxin 生成에 關한 硏究

김학성,민봉희 대구대학교 기초과학연구소 1988 基礎科學硏究 Vol.5 No.-

This study was carried out to investigate the trichothecene mycotoxin productivity of Fusarium tricinctum NRRL 3229. F. tricinctum was cultured on cereal grains at temperature from 10℃ to 15℃ for 4 weeks. Toxins were extracted with solvent and purified partially by column chromatography. The amounts of T-2 toxin, HT-2 toxin, diacetoxyscirpenol and T-2 tetraol were quantitated by gas-liquid chromatography. Corn and millet had the highest productivity of trochothecene mycotoxin. The highest levels of T-2 toxin and HT-2 toxin were produced on corn and maximal yield of T-2 tetraol was obtained in cultures on millet. However, no detectable amount of diacetoxyscirpenol was observed in this experiment. Metabolism of T-2 toxins showed high differences among cereal grains. T-2 toxin was metabolized easily into HT-2 toxin in corn, polished rice, glutinous rice, and sorghum and metabolized very slowly into T-2 tetraol toxin. However, T-2 toxin in unpolished rice was metabolized into HT-2 toxin and T-2 tetraol. Also, T-2 toxin was metabolized rapidly into HT-2 toxin and T-2 tetraol in barley, wheat, millet and red bean while T-2 toxin in unpolished rice was metabolized very slowly into HT-2 toxin and T-2 tetraol.

세라믹필터 역세정에 노즐 및 디퓨저 형상이 미치는 영향

김학빈,정재화,조형희 연세대학교 산업기술연구소 2001 논문집 Vol.33 No.1

The present study has been conducted experimentally to optimize the nozzle and diffuser shapes and diameters for dust removal system. Investigation of a dust removal system has been carried out to develop hot gas clean-up technology and to study for feasibility of clean coal technology pilot plant construction. Experiments was conducted to investigate the effects of pulse nozz1e and diffuser shapes on the air entrainments and on the pressure distribution in a particulate removal system. The main experimental parameters are different nozz1e shapes, nozzle diameters and nozzle tip positions from the diffuser upper plane. Especially, two different types of diffusers with a convergent-divergent shape and a divergent shape were tested systematically. Key words : IGCC(석탄가스화 복합발전), Ceramic Candle Filter(세라믹 캔들 필터), Reverse Cleaning(역세정). Nozz1e(노즐), Diffuser(디퓨저), Entrainment Ratio(동반류비)

대퇴 원위부 골격근내 혈관종 1예

김희상,김학준,안경희,윤동환,유승돈 경희대학교 2003 慶熙醫學 Vol.19 No.2