High expression of keratin 6C is associated with poor prognosis and accelerates cancer proliferation and migration by modulating epithelial–mesenchymal transition in lung adenocarcinoma

Hai‑Bo Hu,Xiao‑Ping Yang,Pei‑Xia Zhou,Xin‑Ai Yang,Bin Yin 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Background Lung adenocarcinoma (LUAD) is a more frequent subtype of lung cancer and most cases are discovered in the late stages. The proliferation and metastasis of LUAD are pivotal for disease progression. Despite unremitting deeper understanding of LUAD biology, the mechanisms involved in the proliferation and metastasis of LUAD remain unclear. The objective of our article was to inquiry the expression and the function of keratin 6C (KRT6C) in LUAD cells. Methods First, the expression level and prognostic value of KRT6C in LUAD tissues were analyzed on the basis of the data acquired from TCGA database. Through qRT-PCR, the expression level of KRT6C on LUAD cell lines (A549, H1299, PC-9) and human normal lung cell line MRC-5 was tested. After that, CCK8 and colony formation assays was utilized to detect cell proliferation. In addition, to explore the influence of KRT6C on LUAD migration and invasion ability, scratch wound healing and transwell assays were utilized. Through western blotting, the protein expression levels of KRT6C, PCNA, E-cadherin, N-cadherin, Snail and Vimentin were detected. Results The outcomes revealed that KRT6C was highly expressed in LUAD tissues and cell lines. Besides, elevated level of KRT6C was related to worse prognosis in LUAD patients. Ablation of KRT6C restrained proliferation, migration and invasion of A549 cells. KRT6C deficiency augmented the expression of E-cadherin as well as reduced the expression of N-cadherin, Snail and Vimentin. Conclusion Above all, these consequences indicated that depletion of KRT6C suppressed A549 cell proliferation, migration and invasion, which might be achieved by regulating EMT. In general, KRT6C is identified as a potential therapeutic target for LUAD.

Correlation of UGT2B7 Polymorphism with Cardiotoxicity in Breast Cancer Patients Undergoing Epirubicin/Cyclophosphamide-Docetaxel Adjuvant Chemotherapy

Hai Li,Bo Hu,Zhe Guo,Xueqing Jiang,Xinyu Su,Xiaoyi Zhang 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.1

Purpose: The present study aimed to investigate correlations between uridine glucuronosyltransferase 2B7 (UGT2B7) -161 singlenucleotide polymorphism C to T (C>T) and the occurrence of cardiotoxicity in Chinese breast cancer (BC) patients undergoingepirubicin/cyclophosphamide-docetaxel (EC-D) adjuvant chemotherapy. Materials and Methods: 427 BC patients who had underwent surgery were consecutively enrolled in this prospective cohortstudy. All patients were scheduled to receive EC-D adjuvant chemotherapy regimen, and they were divided into UGT2B7 -161 CC(n=141), UGT2B7 -161 CT (n=196), and UGT2B7 -161 TT (n=90) groups according to their genotypes. Polymerase chain reactionwas performed for determination of UGT2B7 -161 genotypes. Cardiotoxicity was defined as an absolute decline in left ventricularejection fraction (LVEF) of at least 10% points from baseline to a value less than 53%, heart failure, acute coronary artery syndrome,or fatal arrhythmia. Results: LVEF values were lower at cycle (C) 4, C8, 3 months after chemotherapy (M3), M6, M9, and M12 compared to C0 (allp<0.001), in BC patients undergoing EC-D adjuvant chemotherapy. Cardiotoxicity was recorded for 4.2% of the overall populationand was lowest in the UGT2B7 -161 TT group (1.1%), compared to UGT2B7 -161 CT (3.1%) and UGT2B7 -161 CC (7.8%) group(p=0.026). Multivariate logistic regression revealed that UGT2B7 -161 T allele could independently predict a low occurrence ofcardiotoxicity in BC patients undergoing EC-D adjuvant chemotherapy (p=0.004). Conclusion: A UGT2B7 -161 T allele serves as a potential biomarker for predicting a low occurrence of cardiotoxicity in BC patientsundergoing EC-D adjuvant chemotherapy.

Suppression of Ku80 Correlates with Radiosensitivity and Telomere Shortening in the U2OS Telomerase-negative Osteosarcoma Cell Line

Hu, Liu,Wu, Qin-Qin,Wang, Wen-Bo,Jiang, Huan-Gang,Yang, Lei,Liu, Yu,Yu, Hai-Jun,Xie, Cong-Hua,Zhou, Yun-Feng,Zhou, Fu-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Ku70/80 heterodimer is a central element in the nonhomologous end joining (NHEJ) DNA repair pathway, Ku80 playing a key role in regulating the multiple functions of Ku proteins. It has been found that the Ku80 protein located at telomeres is a major contributor to radiosensitivity in some telomerase positive human cancer cells. However, in ALT human osteosarcoma cells, the precise function in radiosensitivity and telomere maintenance is still unknown. The aim of this study was to investigate the effects of Ku80 depletion in the U2OS ALT cell line cell line. Suppression of Ku80 expression was performed using a vector-based shRNA and stable Ku80 knockdown in cells was verified by Western blotting. U2OS cells treated with shRNA-Ku80 showed lower radiobiological parameters (D0, Dq and SF2) in clonogenic assays. Furthermore, shRNA-Ku80 vector transfected cells displayed shortening of the telomere length and showed less expression of TRF2 protein. These results demonstrated that down-regulation of Ku80 can sensitize ALT cells U2OS to radiation, and this radiosensitization is related to telomere length shortening.

Percutaneous catheter drainage for abscess after surgery

Wen-Bo Zhu,Xiao-Hui Zhao,Hai-Liang Li,Chen-Yang Guo,Quan-Jun Yao,Xiang Geng,Ke Zhao,Hong-Tao Hu 소화기인터벤션의학회 2022 Gastrointestinal Intervention Vol.11 No.4

Percutaneous abscess drainage (PAD) has been proven to be a safe, effective, and widely used technique for the treatment of patients with abscesses after gastrointestinal surgery. The indications for PAD are expanding and most postoperative abscesses of the gastrointestinal tract are susceptible to PAD. PAD uses various imaging methods, including ultrasound, computed tomography, and several safe and reliable catheter insertion methods.

Benzochloroporphyrin Derivative Induced Cytotoxicity and Inhibition of Tumor Recurrence During Photodynamic Therapy for Osteosarcoma

Gong, Hai-Yang,Sun, Meng-Xiong,Hu, Shuo,Tao, Ying-Ying,Gao, Bo,Li, Guo-Dong,Cai, Zheng-Dong,Yao, Jian-Zhong Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Photodynamic therapy (PDT) is a promising cancer treatment modality that uses dye-sensitized photooxidation of biologic matter in target tissue. This study explored effects of the photosensitizer BCPD-17 during PDT for osteosarcoma. LM-8 osteosarcoma cells were treated with BCPD-17 and cell viability after laser irradiation was assessed in vitro with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effects of BCPD-17 during PDT recurrence were then examined on tumor-bearing mice in vivo. BCPD-17 had dosedependent cytotoxic effects on LM-8 osteosarcoma cells after laser irradiation which also had energy-dependent effects on the cells. The rate of local recurrence was reduced when marginal resection of mice tumors was followed by BCPD-17-mediated PDT. Our results indicated BCPD-17-mediated PDT in combination with marginal resection of tumors is a potentially new effective treatment for osteosarcoma.

Percutaneous catheter drainage for abscess after surgery

Wen-Bo Zhu,Xiao-Hui Zhao,Hai-Liang Li,Chen-Yang Guo,Quan-Jun Yao,Xiang Geng,Ke Zhao,Hong-Tao Hu 소화기인터벤션의학회 2022 International journal of gastrointestinal interven Vol.11 No.4

Percutaneous abscess drainage (PAD) has been proven to be a safe, effective, and widely used technique for the treatment of patients with abscesses after gastrointestinal surgery. The indications for PAD are expanding and most postoperative abscesses of the gastrointestinal tract are susceptible to PAD. PAD uses various imaging methods, including ultrasound, computed tomography, and several safe and reliable catheter insertion methods.

Modal parameter identification of civil structures using symplectic geometry mode decomposition

Feng Hu,Lun-hai Zhi,Zhixiang Huang,Bo Chen 한국풍공학회 2023 Wind and Structures, An International Journal (WAS Vol.36 No.1

In this article, a novel structural modal parameters identification methodology is developed to determine the natural frequencies and damping ratios of civil structures based on the symplectic geometry mode decomposition (SGMD) approach. The SGMD approach is a new decomposition algorithm that can decompose the complex response signals with better decomposition performance and robustness. The novel method firstly decomposes the measured structural vibration response signals into individual mode components using the SGMD approach. The natural excitation technique (NExT) method is then used to obtain the free vibration response of each individual mode component. Finally, modal natural frequencies and damping ratios are identified using the direct interpolating (DI) method and a curve fitting function. The effectiveness of the proposed method is demonstrated based on numerical simulation and field measurement. The structural modal parameters are identified utilizing the simulated non-stationary responses of a frame structure and the field measured non-stationary responses of a supertall building during a typhoon. The results demonstrate that the developed method can identify the natural frequencies and damping ratios of civil structures efficiently and accurately.

Leader-Follower Consensus for a Class of Nonlinear Multi-Agent Systems

Xing-Hu Wang,Hai-Bo Ji 제어·로봇·시스템학회 2012 International Journal of Control, Automation, and Vol.10 No.1

This paper deals with the leader-follower consensus problem for a class of nonlinear multi-agent sys-tems. All agents have identical nonlinear dynamics in the strict feedback form with Lipschitz growth condition. Both full state consensus protocol and dynamic output consensus protocol are provided. It is shown that under a connected undirected information communication topology, the proposed protocols can solve the leader-follower consensus problem. Two consensus protocol design procedures are presented and a numerical example is given to illustrate the proposed protocols.

Granular Effects on Depositional Processes of Debris Avalanches

Yu-xiang Hu,Hai-bo Li,Shun-chao Qi,Gang Fan,Jia-wen Zhou 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.4

Debris avalanches generally have massive mobility and extensive deposited area, which have strong relation to the granular effect. The granular effect is an outcome from the interaction (based on frictional mechanism) among particles. Discrepancy of grain composition, for instance the difference of particle size and their proportion of particles, is a key factor to influence granular effect. A series of laboratory tests have been conducted to research the granular effects on depositional processes of debris avalanches containing fine- and coarse-grained particles. The velocity of debris front, the morphology characteristics and runout distance of the debris avalanche are considered in detail, and the mass parameter and the size parameter are introduced in analysis for the research of granular effect. The experimental results indicate that the granular effects can decrease the dissipation of momentum and increase the accumulation region, leading to further runout distance and more extensive deposited areas. Further, the velocity, the range of depositions of debris avalanches are larger than that in other conditions when the proportion of fine-grained particles is 30%, especially the materials consist of heterogeneous granular. It means there have a critical mass parameter in experiments which lead to the high efficiency of granular effects, and the granular effect is weakening in homogeneous granular composition.

Transposon <i>Ac/Ds</i> -induced chromosomal rearrangements at the rice <i>OsRLG5</i> locus

Xuan, Yuan Hu,Piao, Hai Long,Je, Byoung Il,Park, Soon Ju,Park, Su Hyun,Huang, Jin,Zhang, Jian Bo,Peterson, Thomas,Han, Chang-deok Oxford University Press 2011 Nucleic acids research Vol.39 No.22

<P>Previous studies have shown that pairs of closely-linked <I>Ac/Ds</I> transposable elements can induce various chromosomal rearrangements in plant genomes. To study chromosomal rearrangements in rice, we isolated a line (<I>OsRLG5-161)</I> that contains two inversely-oriented <I>Ds</I> insertions in <I>OsRLG5</I> (<I>Oryza sativa</I> Receptor like kinase Gene 5). Among approximately 300 plants regenerated from <I>OsRLG5-161</I> heterozygous seeds, 107 contained rearrangements including deletions, duplications and inversions of various sizes. Most rearrangements were induced by previously identified alternative transposition mechanism. Furthermore, we also detected a new class of rearrangements that contain juxtaposed inversions and deletions on the same chromosome. We propose that these novel alleles were generated by a previously unreported type of alternative transposition reactions involving the 5′ and 3′ termini of two inversely-oriented <I>Ds</I> elements located on the same chromatid. Finally, 11% of rearrangements contained inversions resulting from homologous recombination between the two inverted <I>Ds</I> elements in <I>OsRLG5-161</I>. The high frequency inheritance and great variety of rearrangements obtained suggests that the rice regeneration system results in a burst of transposition activity and a relaxation of the controls which normally limit the transposition competence of individual <I>Ds</I> termini. Together, these results demonstrate a greatly enlarged potential of the <I>Ac/Ds</I> system for plant chromosome engineering.</P>