Problematic Expansion on Jurisdiction: Some Observation on the South China Sea Arbitration

Xiaoyi Zhang 이준국제법연구원 2016 Journal of East Asia and International Law Vol.9 No.2

Observer-based Adaptive Bipartite Time-varying Formation Tracking of Multi-agent System with Bounded Unknown Input

Xiaoyi Zhang,Jie Wu,Xisheng Zhan,Tao Han,Huaicheng Yan 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.12

In this paper, the bipartite time-varying formation tracking (BTVFT) problem is studied for multi-agent system (MAS) with the state of agents is unpredictable and a tracking leader of unknown bounded control input. Based on available states and unavailable states, a innovative kind of adaptive BTVFT protocols for MAS is respectively proposed in this article. Then by using Lyapunov stability theory, the result implies that under the appropriate assumptions, one group of followers will trace the leader agent and achieve given formation, while another group of followers will accomplish a given formation at the opposite to the origin. Furthermore, simulation example is represented to validate the performance and effectiveness of the design techniques.

Flexural Behavior of RC Beams with NSM Prestressed Helical Rib Steel Wire

Meixiang Zhang,Xiaolin Yang,Yahong Ding,Ping Xu,Bo Sun,Xiaoyi Zhang,Xianggang Zhang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.9

The reinforcement technique of near-surface mounted?NSM?prestressed helical rib steel wire (PHRSW) with wide raw material sources, lower in price and high application value can significantly enhance the flexural behavior of reinforced-concrete (RC) structures. Based on 8 flexural RC beams reinforced with NSM PHRSW, performance of test beams under different groove widths (10 mm, 15 mm and 20 mm) and concrete strengths (C30 and C40) was analyzed, including load-deflection, ductility, flexural capacity, strain of longitudinal steel bar and HRSW, failure mode and crack propagation. The results show that the overall flexural behavior RC beams reinforced with NSM PHRSW was significantly improved. There is a good entirety bearing capacity between the longitudinal reinforcement and PHRSW of reinforced beam with suitable groove size. The comprehensive performance of reinforced beams increases first then decreases, with the increase of groove size. The higher the strength of concrete, the more significant the influence of groove size. There is an optimal match between groove width and concrete strength for RC beams reinforced with NSM PHRSW. Considering comprehensive property of reinforced beams, groove width with 15mmwas best in this research. The results can make the research of RC beams reinforced with NSM PHRSW more comprehensive and in-depth, and provide design basis for its engineering application.

MicroRNA-455-3p promotes TGF-β signaling and inhibits osteoarthritis development by directly targeting PAK2

Shu Hu,Xiaoyi Zhao,Guping Mao,Ziji Zhang,Xingzhao Wen,Chengyun Zhang,Weiming Liao,Zhiqi Zhang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

MicroRNAs (miRNAs, miR) play a key role in the pathogenesis of osteoarthritis (OA). Few studies have examined theregulatory role of P21-activated kinases (PAKs), a family of serine/threonine kinases, in OA. The aim of this study was todetermine whether miR-455-3p can regulate cartilage degeneration in OA by targeting PAK2. MiR-455-3p knockoutmice showed significant degeneration of the knee cartilage. MiR-455-3p expression increased and PAK2 expressiondecreased in the late stage of human adipose-derived stem cell (hADSC) chondrogenesis and in chondrocytesaffected by OA. Furthermore, in both miR-455-3p-overexpressing chondrocytes and PAK2-suppressing chondrocytes,cartilage-specific genes were upregulated, and hypertrophy-related genes were downregulated. A luciferase reporterassay confirmed that miR-455-3p regulates PAK2 expression by directly targeting the 3′-untranslated regions (3′UTRs)of PAK2 mRNA. IPA-3, a PAK inhibitor, inhibited cartilage degeneration due to OA. Moreover, suppressing PAK2promoted R-Smad activation in the TGF/Smad signaling pathway in chondrocytes. Altogether, our results suggest thatmiR-455-3p promotes TGF-β/Smad signaling in chondrocytes and inhibits cartilage degeneration by directlysuppressing PAK2. These results thus indicate that miR-455-3p and PAK2 are novel potential therapeutic agents andtargets, respectively, for the treatment of OA.

Synchronization and anti-synchronization of fractional dynamical networks

Zhang, Runfan,Chen, Diyi,Do, Younghae,Ma, Xiaoyi SAGE Publications 2015 Journal of vibration and control Vol.21 No.16

<P>The issue of synchronization between dynamical systems has attracted much attention, and the systems with integer-order dynamical networks have been well studied. The synchronous behavior of fractional-order dynamical systems is very interesting and importance, but has rarely been studied. In this paper, we studied the synchronization and anti-synchronization behavior between integer-order dynamical networks and fractional-order dynamical systems via a Takagi-Sugeno fuzzy model. Remarkably, there is synchronous behavior in such a system, and this is dramatically different from the behavior of integer-order dynamical networks. Moreover, we studied the impact of different coupling strengths on the dynamical process of synchronization and robustness of the designed controller to different coupling functions, different dimensions of dynamical equations and different fractional orders. Finally, we propose the theoretical analysis, which coincides well with the numerical simulations of five typical examples.</P>

miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro

Xiaoyi Hu,Jiyu Miao,Min Zhang,Xiaofei Wang,Zhenzhen Wang,Jia Han,Dongdong Tong,Chen Huang 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.5

Studies have revealed that miR-103a-3p contributes to tumor growth in several human cancers, and high miR-103a-3p expression is associated with poor prognosis in advanced gastric cancer (GC) patients. Moreover, bioinformatics analysis has shown that miR-103a-3p is upregulated in The Cancer Genome Atlas (TCGA) stomach cancer cohort. These results suggest that miR-103a-3p may function as an oncogene in GC. The present study aimed to investigate the role of miR-103a-3p in human GC. miR-103a-3p expression levels were increased in 33 clinical GC specimens compared with adjacent nontumor stomach tissues. Gain- and loss-of-function studies were performed to identify the correlation between miR-103a-3p and tumorigenesis in human GC. Inhibiting miR-103a-3p suppressed GC cell proliferation and blocked the S-G2/M transition in MKN-45/SGC-7901 cells, whereas miR-103a-3p overexpression improved GC cell proliferation and promoted the S-G2/M transition in vitro. Bioin-formatics and dual-luciferase reporter assays confirmed that ATF7 is a direct target of miR-103a-3p. Analysis of the TCGA stomach cancer cohort further revealed that miR-103a-3p expression was inversely correlated with ATF7 expression. Notably, silencing ATF7 showed similar cellular and molecular effects as miR-103a-3p overexpression, namely, increased GC cell proliferation, improved CDK2 expression and decreased P27 expression. ATF7 overexpression eliminated the effects of miR-103a-3p expression. These findings indicate that miR-103a-3p promotes the proliferation of GC cell by targeting and suppressing ATF7 in vitro.

miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro

Hu, Xiaoyi,Miao, Jiyu,Zhang, Min,Wang, Xiaofei,Wang, Zhenzhen,Han, Jia,Tong, Dongdong,Huang, Chen Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.5

Studies have revealed that miR-103a-3p contributes to tumor growth in several human cancers, and high miR-103a-3p expression is associated with poor prognosis in advanced gastric cancer (GC) patients. Moreover, bioinformatics analysis has shown that miR-103a-3p is upregulated in The Cancer Genome Atlas (TCGA) stomach cancer cohort. These results suggest that miR-103a-3p may function as an oncogene in GC. The present study aimed to investigate the role of miR-103a-3p in human GC. miR-103a-3p expression levels were increased in 33 clinical GC specimens compared with adjacent nontumor stomach tissues. Gain- and loss-of-function studies were performed to identify the correlation between miR-103a-3p and tumorigenesis in human GC. Inhibiting miR-103a-3p suppressed GC cell proliferation and blocked the S-G2/M transition in MKN-45/SGC-7901 cells, whereas miR-103a-3p overexpression improved GC cell proliferation and promoted the S-G2/M transition in vitro. Bioinformatics and dual-luciferase reporter assays confirmed that ATF7 is a direct target of miR-103a-3p. Analysis of the TCGA stomach cancer cohort further revealed that miR-103a-3p expression was inversely correlated with ATF7 expression. Notably, silencing ATF7 showed similar cellular and molecular effects as miR-103a-3p overexpression, namely, increased GC cell proliferation, improved CDK2 expression and decreased P27 expression. ATF7 overexpression eliminated the effects of miR-103a-3p expression. These findings indicate that miR-103a-3p promotes the proliferation of GC cell by targeting and suppressing ATF7 in vitro.

Surface layer reflective index changes of Au nanoparticle functionalized porous silicon microcavity for DNA detection

Hongyan Zhang,Zhenhong Jia,Xiaoyi Lv 한국물리학회 2015 Current Applied Physics Vol.15 No.8

A technique is demonstrated to detect DNA hybridization based on surface layer of Au/porous silicon microcavity (Au/PSM) substrate for very small amount of biomolecules. Simulations show that the increase of effective refractive index for the first layer of PSM will cause a blue shift for its reflectance spectrum, and the blue shift becomes less with the increase of refractive index for one more layers. In experiments, such a blue shift of reflectance spectrum of PSM comes from the increase of refractive index by DNA hybridization on the surface. The detection limit of Au/PSM biosensor is 15.15 nM for 19-base pair DNA, which is comparable to that of reported biosensors based on porous silicon (PS). Therefore such an Au/PSM could be very useful to develop simple, rapid and sensitive optical biosensors when the amount of target is very small.

Maturation Disparity between Hand-Wrist Bones in a Chinese Sample of Normal Children: An Analysis Based on Automatic BoneXpert and Manual Greulich and Pyle Atlas Assessment

Ji Zhang,Fangqin Lin,Xiaoyi Ding 대한영상의학회 2016 Korean Journal of Radiology Vol.17 No.3

Objective: To assess the maturation disparity of hand-wrist bones using the BoneXpert system and Greulich and Pyle (GP) atlas in a sample of normal children from China. Materials and Methods: Our study included 229 boys and 168 girls aged 2–14 years. The bones in the hand and wrist were divided into five groups: distal radius and ulna, metacarpals, proximal phalanges, middle phalanges and distal phalanges. Bone age (BA) was assessed separately using the automatic BoneXpert and GP atlas by two raters. Differences in the BA between the most advanced and retarded individual bones and bone groups were analyzed. Results: In 75.8% of children assessed with the BoneXpert and 59.4% of children assessed with the GP atlas, the BA difference between the most advanced and most retarded individual bones exceeded 2.0 years. The BA mean differences between the most advanced and most retarded individual bones were 2.58 and 2.25 years for the BoneXpert and GP atlas methods, respectively. Furthermore, for both methods, the middle phalanges were the most advanced group. The most retarded group was metacarpals for BoneXpert, while metacarpals and the distal radius and ulna were the most retarded groups according to the GP atlas. Overall, the BAs of the proximal and distal phalanges were closer to the chronological ages than those of the other bone groups. Conclusion: Obvious and regular maturation disparities are common in normal children. Overall, the BAs of the proximal and distal phalanges are more useful for BA estimation than those of the other bone groups.

Antifreeze protein detection using Rhodamine B as photoluminescence label in porous silicon

Hongyan Zhang,Zhenhong Jia,Xiaoyi Lv,Junwei Hou,Xiaojing Liu,Ji Ma,Jun Zhou 한국물리학회 2013 Current Applied Physics Vol.13 No.4

A novel method is demonstrated to detect Antifreeze proteins (AFPs) based on photoluminescence (PL)using porous silicon (PS) coated with silver as a substrate. Ag/PS substrate is obtained through immersion of PS in silver nitrate (AgNO3) solutions and is incubated with Rhodamine B (RB) as PL label. This substrate is easy to be fabricated and the pore size of PS is large enough for biological molecules to infiltrate, which is an ideal platform for biological molecule detection. Through functionalization used glutaraldehyde (GTA) and 4-(N-Maleimidomethyl) cyclohexane-1-carboxylicacid (Sulfo-SMCC) as crosslinkers separately, we test the role of the AFPs antibodies in selective capturing the AFPs antigen and explain the reason of the enhancement of PL intensity. The result shows a significant enhancement of the PL intensity of RB at around 590 nm due to the interaction of antibodyeantigen competitive binding with AFPs. Therefore, the PL corresponding to RB was selected to detect the target AFPs and the PL intensity of RB proportional to the AFPs concentration. The detection limit was found to be 1.65 mg/ml for AFPs when GTA was used as cross-linker, and the detection limit was 16.5 ng/ml with Sulfo-SMCC as cross-linker