Angio-Vasculogenic Properties of Endothelial-Induced Mesenchymal Stem Cells Derived From Human Adipose Tissue

Guo, Long Zhe,Kim, Tae-Hee,Han, Seongho,Kim, Sung-Whan UNKNOWN 2016 CIRCULATION JOURNAL Vol.80 No.4

<P>Background: Although stem cells have been regarded as a promising therapeutic option, the marginal therapeutic effects of stem cells are limitations that must be overcome for the development of effective cell therapy. This study sought to identify the angio-vasculogenic properties of endothelial differentiated mesenchymal stem cells (MSCs) and to determine whether these cells are effective for vascular repair. Methods and Results: Adipose MSCs were cultured for 10 days under endothelial cell (EC) culture conditions. These endothelial cell differentiated adipose MSCs (EA) and undifferentiated adipose MSCs (UA) were characterized via angiogenesis and adhesion assays. These cells were transplanted into a hindlimb ischemia (HLI) model to determine therapeutic effects and their underlying mechanisms. EA displayed low adhesion and angiogenic properties in vitro compared with UA. When implanted into mouse HLI models, EA exhibited the decreased recovery of blood perfusion in limb ischemia than uncultured UA. Histology data showed that injected EA exhibited lower retention, angiogenic cytokine levels, and neovascularization in vivo than did UA. Short-term differentiated EA display less cell engraftment and angio-vasculogenic potential, and are less effective for peripheral vascular repair than UA. Conclusions: EC differentiation of MSCs may not present an effective strategy for the promotion of therapeutic neovascularization.</P>

On the Path of Acquiring in Sense and Sensibility

( Guo Zhe ) 현대영미어문학회 2019 현대영미어문학회 추계학술대회 발표논문집 Vol.2019 No.-

Since 1811 Sense and Sensibility was published, it is well-liked by readers at home and abroad. There is an inseparable connection between artistic works and the ideology generated by the society which is also reflected through the depiction of social life in Jane Austen’s novel. The paper discusses the in the forming acquisitive society, the traditional behavior code was challenged by the new preoccupation in promote the acquisition of wealth and how this phenomenon is manifested in the novel.

Thienopyridine reloading in clopidogrel-loaded patients undergoing percutaneous coronary interventions: The PRAISE study

Guo, Long Zhe,Kim, Moo Hyun,Shin, Eun Seok,Ann, Soe Hee,De Jin, Cai,Cho, Young-Rak,Park, Jong Sung,Park, Kyungil,Park, Tae-Ho,Lee, Michael S.,Serebruany, Victor L. Elsevier 2016 INTERNATIONAL JOURNAL OF CARDIOLOGY Vol.222 No.-

<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>The impact of thienopyridine reloading on clinical outcomes, and residual high platelet reactivity (HPR) is unclear. We sought to compare the HRP-related effect of prasugrel and clopidogrel reloading in the already clopidogrel-loaded patients undergoing percutaneous coronary intervention (PCI).</P> <P><B>Materials and methods</B></P> <P>In this prospective, two-center, randomized, open-label study, patients with HPR who had undergone PCI after a clopidogrel (300–600mg) loading dose (LD) were enrolled. Among screened (n=153), HPR was determined in seventy-six patients, who were randomized to either repeated clopidogrel (300mg LD, followed by 75mg MD daily) or prasugrel (20mg LD, followed by 5mg MD daily). The primary endpoint was HPR at 24h after PCI, as determined by the VerifyNow assay. The rates of sustained high and low platelet reactivity, periprocedural myocardial injury (PMI) and 30-day clinical outcomes were also assessed.</P> <P><B>Results</B></P> <P>Higher inhibition of platelet reactive units (PRU) was observed in the prasugrel group than after clopidogrel reloading (Pre-PCI: 284.4±32.0 vs 279.5±32.5, p=0.504; Post-PCI: 100.0±67.0 vs 202.9±65.8, p<0.001; 30days: 170.8±69.8 vs 215.1±62.4, p=0.007). There were less HRP post-PCI after prasugrel compared with the clopidogrel group (2.7 vs 36.1%, p<0.001). However, reloading with prasugrel did not reduce PMI compared to clopidogrel (36.8% vs 39.5%, p=0.813).</P> <P><B>Conclusion</B></P> <P>Prasugrel reloading led to a greater reduction in HPR, but similar with clopidogrel PMI in post-PCI patients. Larger randomized evidence is needed for optimization of loading strategies with thienopyridines.</P> <P>Clinical Trial Registration Information: NCT01609647.</P>

Comparison of Three Tests to Distinguish Platelet Reactivity in Patients with Renal Impairment during Dual Antiplatelet Therapy

Guo, Long Zhe,Kim, Moo Hyun,Kim, Tae Hyung,Park, Jong Seong,Jin, Enze,Shim, Chang Heon,Choi, Sun Young,Serebruany, Victor L. S. Karger AG 2016 Nephron Vol.132 No.3

<P>Background: Clopidogrel and aspirin combination remains a cornerstone for modern dual antiplatelet therapy (DAPT) following coronary stenting. Although monitoring is not currently recommended, certain high-risk cohorts may benefit from tailoring antiplatelet options to reduce thrombotic or/and hemorrhagic risks. Patients with diminished estimated glomerular filtration rate (eGFR) are prone to both vascular occlusions and bleeding events in whom monitoring may be especially advantageous. We compared the residual platelet reactivity assessed by 3 conventional tests during the maintenance antiplatelet therapy dependent on eGFR. Methods: Post-stenting patients (n = 701) receiving aspirin 100 mg/ daily and clopidogrel 75 mg/daily were prospectively enrolled in the cross-sectional single-center study. Patients were dichotomized into 5 groups: eGFR >90, 60-89, 30-59, <30 ml/min/1.73 m(2), and dialysis. Platelet reactivity by VerifyNow (TM), light transmittance aggregometry (LTA), and Multiplate analyzer by multiple electrode platelet aggregometry (MEA) assays together with eGFR calculations were done simultaneously at 1 month after coronary stenting. Results: VerifyNow assay distinguished residual platelet reactivity dependent on eGFR deterioration (191 +/- 72 vs. 216 +/- 78 vs. 248 +/- 80 vs. 264 +/- 70 vs. 317 +/- 96 PRU; p < 0.001). In contrast, LTA (34.3 +/- 18.1 vs. 34.7 +/- 18.1 vs. 38.0 +/- 16.6 vs. 33.0 +/- 17.3 vs. 34.1 +/- 29.3%; p = 0.242), or MEA (37.2 +/- 19.6 vs. 33.8 +/- 18.4 vs. 38.6 +/- 21.4 vs. 36.5 +/- 20.5 vs. 38.3 +/- 28.3 AU/min; p = 0.086) failed to triage platelet reactivity in renal patients. Agreement among assays to identify patients with impaired platelet reactivity and eGFR during antiplatelet therapy was low. The multivariable regression analyses confirmed the VerifyNow advantage, since the differences in the platelet reactivity were highly significant for all renal impairment (RI) groups. In contrast, LTA did not distinguish RI patients, and for the MEA, only RI5 (dialysis) cohort exhibit borderline significant decline of residual platelet reactivity. Conclusion: Among 3 assays, VerifyNow was capable to reliably triage residual platelet reactivity in post-stenting DAPT patients dependent on the gradual decline of eGFR during therapy with clopidogrel and aspirin. These data should be confirmed in a large validation longitudinal trial, and may justify future platelet activity monitoring for potential regimen/dose adjustment in high-risk patients. The clinical implications of these data are still unclear, but may give an indication as to whether or when DAPT dose adjustment will become a reality. (C) 2016 S. Karger AG, Basel</P>

Low Microsomal Epoxide Hydrolase Expression is Associated with Bladder Carcinogenesis and Recurrence

Zhang, Zhe,Yu, Xiu-Yue,Zhang, Guo-Jun,Guo, Kun-Feng,Kong, Chui-Ze Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

Microsomal epoxide hydrolase (mEH) plays a significant role in the metabolism of numerous xenobiotics and is associated with several forms of cancer. Here, we investigated the role of mEH expression in bladder carcinogenesis, subsequent progression and recurrence. The expression of mEH was analyzed by Western blot in 50 bladder urothelial carcinoma and 20 normal epithelial tissues. There was a significantly higher mEH expression in the normal epithelium (P<0.05) and mEH expression was lower in high stage than in low stage tumors (P<0.05). Further, immunohistochmistry in 106 bladder urothelial carcinoma demonstrated mEH expression to be negatively correlated with histological grade, pT stage and recurrence (P<0.05). These findings suggest the important role of mEH in bladder carcinogenesis, cancer development and recurrence, providing support for efforts to develop mEH-based gene therapy.

Combined fine-needle aspiration with core needle biopsy for assessing thyroid nodules: a more valuable diagnostic method?

Zhe Chen,Jia-jia Wang,Dong-ming Guo,Yu-xia Zhai,Zhuo-zhi Dai,Hong-hui Su 대한초음파의학회 2023 ULTRASONOGRAPHY Vol.42 No.2

Purpose: This study aimed to evaluate the diagnostic value of combined fine-needle aspiration (FNA) with core needle biopsy (CNB) in thyroid nodules. Methods: FNA and CNB were performed simultaneously on 703 nodules. We compared the proportions of inconclusive results and the diagnostic performance for malignancy among FNA, CNB, and combined FNA/CNB for different nodule sizes. Results: Combined FNA/CNB showed lower proportions of inconclusive results than CNB for all nodules (2.8% vs. 5.7%, P<0.001), nodules ≤1.0 cm (4.9% vs. 7.3%, P=0.063), nodules >1.0 cm (2.0% vs. 5.0 %, P<0.001), nodules ≤1.5 cm (3.8% vs. 7.9 %, P<0.001), and nodules >1.5 cm (2.1% vs. 3.9 %, P=0.016). The sensitivity of combined FNA/CNB in predicting malignancy was significantly higher than that of CNB (89.0% vs. 80.0%, P<0.001) and FNA (89.0% vs. 58.1%, P<0.001) for all nodules. Within American College of Radiology Thyroid and Imaging Reporting and Data System grades 4-5, in the subgroup of nodules ≤1.5 cm, combined FNA/ CNB showed the best sensitivity in predicting malignancy (91.4%), significantly higher than that of CNB (81.0%, P<0.001) and FNA (57.8%, P<0.001). However, in the subgroup of nodules >1.5 cm, the difference between combined FNA/CNB and CNB was not significant (84.2% vs. 78.9%, P=0.500). Conclusion: Regardless of nodule size, combined FNA/CNB tended to yield lower proportions of inconclusive results than CNB or FNA alone and exhibited higher performance in diagnosing malignancy. The combined FNA/CNB technique may be a more valuable diagnostic method for nodules ≤1.5 cm and nodules with a risk of malignancy than CNB and FNA alone.

Development of High-specificity Antibodies against Renal Urate Transporters Using Genetic Immunization

( Guo Shuang Xu ),( Xiang Mei Chen ),( Di Wu ),( Suo Zhu Shi ),( Jian Zhong Wang ),( Rui Ding ),( Quan Hong ),( Zhe Feng ),( Shu Peng Lin ),( Yang Lu ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.6

Variation of Urinary and Serum Trace Elements (Ca, Zn, Cu, Se) in Bladder Carcinoma in China

Guo, Kun-Feng,Zhang, Zhe,Wang, Jun-Yong,Gao, Sheng-Lin,Liu, Jiao,Zhan, Bo,Chen, Zhi-Peng,Kong, Chui-Ze Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Backgrounds: Deficiency or excess of trace elements can induce body metabolic disorders and cellular growth disturbance, even mutation and cancerization. Since there are few studies of the effect of trace elements in bladder carcinoma in China, the aim of this study was thus to assess variation using a case control approach. Methods: To determine this, 81 patients with bladder carcinoma chosen as a study group and 130 healthy persons chosen as a control group were all assayed for urinary and serum trace elements (calcium [Ca], zinc [Zn], copper [Cu], selenium [Se]) using an atomic absorption spectrophotometer, and the results were analyzed by independent sample t tests. The correlative factors on questionnaires answered by all persons were analyzed by logistic regression. Results: The results showed urinary Ca, Zn and serum Cu levels of the study group to be significantly higher (P<0.05) than those of he control group. Serum Ca and Se levels of study group were significantly lower (P<0.05) than those of control group. Conclusion: There were higher urinary Zn and serum Cu concentrations in bladder carcinoma cases. Bladder carcinoma may be associated with Ca metabolic disorder, leading to higher urinary Ca and lower serum Ca. Low serum Se and smoking appear to be other risk factors for bladder carcinoma in China.

Feasibility study of equal type, experienced and compound robots based on reliability theory

Zhe YIN,Yun-fei GUO,Mao-sheng LAI 제어로봇시스템학회 2011 제어로봇시스템학회 국제학술대회 논문집 Vol.2011 No.10

Through the introduction of the concept of reliability, events encountered by the robot are divided into three cases: the first is the one that the robot to can be sure to judge and finish the event, the second is that the robot can certainly not be able to determine if the events should be done. In both cases above the robot will be able to make a positive assessment; however, there is a fuzzy event, namely: the robot can not make sure, that this is the need to issue a request to the controller to get the new instruction, and then give a specific judge.

Leader-following Non-fragile Consensus Control of Fuzzy Multi-agent Fractional Order Interval Systems

Zhe Wang,Dingyu Xue,Xuefeng Zhang,Qing-Guo Wang 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.2

The consensus control of fuzzy multi-agent fractional order interval systems (MA-FOISs) is proposed in this brief. Firstly, a new model of fuzzy MA-FOISs based on the non-fragile observer is presented. Then, by using the technologies of generalised matrix singular value decomposition (SVD) and linear matrix inequalities (LMIs), the sufficient conditions for determining the consensus of fuzzy MA-FOISs are given through the equivalence of the stability and the consensus of multi-agent systems (MASs). Moreover, when the actual system does not need to be described by the fuzzy set, the consensus conditions for the MA-FOISs are given. Finally, the validity of the results given are illustrated by the numerical example.