The transcription factor FoxM1 activates Nurr1 to promote intestinal regeneration after ischemia/ reperfusion injury

Guo Zu,Jing Guo,Tingting Zhou,Ningwei Che,Baiying Liu,Dong Wang,Xiangwen Zhang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

FoxM1 is involved in the regeneration of several organs after injury and expressed in the intestinal mucosa. The intrinsic mechanism of FoxM1 activity in the mucosa after intestinal ischemia/reperfusion (I/R) injury has not been reported. Therefore, we investigated the role of FoxM1 in mediating intestinal mucosa regeneration after I/R injury. Expression of FoxM1 and the proliferation of intestinal mucosa epithelial cells were examined in rats with intestinal I/R injury and an IEC-6 cell hypoxia/reperfusion (H/R) model. The effects of FoxM1 inhibition or activation on intestinal epithelial cell proliferation were measured. FoxM1 expression was consistent with the proliferation of intestinal epithelial cells in the intestinal mucosa after I/R injury. Inhibition of FoxM1 expression led to the downregulation of Ki67 expression mediated by the inhibited expression of Nurr1, and FoxM1 overexpression promoted IEC-6 cell proliferation after H/R injury through activating Nurr1 expression. Furthermore, FoxM1 directly promoted the transcription of Nurr1 by directly binding the promoter of Nurr1. Further investigation showed low expression levels of FoxM1, Nurr1, and Ki-67 in the intestinal epithelium of patients with intestinal ischemic injury. FoxM1 acts as a critical regulator of intestinal regeneration after I/R injury by directly promoting the transcription of Nurr1. The FoxM1/ Nurr1 signaling pathway represents a promising therapeutic target for intestinal I/R injury and related clinical diseases.

Electrospinning Inorganic/Organic Nanohybridization Membranes with Hydrophobic and Oleophobic Performance

Tingting Zhang,Zihui Li,Ya Liu,Kangjia Ding,Yangfeng Guo,Yiyang Xu,Mengfan Sun,Dongfang Wang,Qian Li 한국섬유공학회 2023 Fibers and polymers Vol.24 No.12

Polytetrafluoroethylene (PTFE) nano-porous membrane has been widely used in various fields due to its excellent thermal stability and chemical stability. However, PTFE nanofiber membranes with simultaneous hydrophobic and oleophobic properties are essential to promote the application of PTFE. Here, based on inorganic/organic nanohybridization, we report a strategy for constructing nanostructures on fibers by adding nanoparticles, such as MOF and ZrO2. The results of FTIR and XRD confirmed MOF was synthesized successfully. At the same time, the SEM results showed UiO-66-(COOH)2 is spherical with an average diameter of 152 nm, and there is no agglomeration, which is suitable for electrospinning. Further, MOF and ZrO2 were payload into PTFE nanofibers. The results of SEM and AFM confirmed nanostructures will be more uniform and pronounced with the increase of UiO-66-(COOH)2 content, and nanostructures are most obvious when the content of UiO-66-(COOH)2 is 15%. The introduced nanostructures can increase the oil contact angle of the PTFE nano-porous membrane to 110° without introducing other groups, and further improve the water contact angle from 133° to 145°. Meanwhile, the introduction of a certain amount of hydrophilia groups can increase the oil contact angle to more than 120°. The simple strategy is of great significance to expand the application of PTFE fiber membrane in dealing with waste water treatment fields.

Recent Changes in Heavy Precipitation Events in Northern Central China and Associated Atmospheric Circulation

Tingting Han,Xinyi Guo,Botao Zhou,Xin Hao 한국기상학회 2021 Asia-Pacific Journal of Atmospheric Sciences Vol.57 No.2

Northern Central China (NC China) is a seriously arid region. Precipitation variations are vital for environmental protection and socio-economical development. This study investigates the recent changes in summer heavy precipitation (HP) events over NC China and associated atmospheric circulation anomalies. Compared with the period of 1986–2002, the HP amount and frequency both significantly increase during 2003–2016, contributing to the shift to increase in total summer precipitation amount. After the early 2000s, the Northwest Pacific subtropical high becomes intensified and shifts westward and northward, leading to significant moisture convergence anomalies over NC China and divergence anomalies over Northwest Pacific. Therefore, the net moisture budget dramatically increases since the early 2000s. Further results show that the strengthened net moisture influx across the southern boundary contributes dominantly to the increased net moisture budget, especially at the lower level. Despite a relatively small magnitude, the intensified westerly current across western boundary makes a dominant contribution at middle and upper layers. Additionally, the strengthened westerly and easterly anomalies occupy northern and southern China, respectively, along with the eastward expansion of westerly jet stream region. Thus, the lower-level convergence and upper-level divergence both intensify, and further trigger enhanced ascending movement. These conditions jointly contribute to the inter-decadal change in HP events over NC China after the early 2000s.

Performance study of g-C3N4/carbon black/BiOBr@Ti3C2/MoS2 photocatalytic fuel cell for the synergistic degradation of different types of pollutants

Guo Huilin,Yu Tingting,Zhao Lei,Qian Jun,Yu Jiahe,Zhang Yu,Teng Yongyue,Zhu Chunshui,Yang Tao,Chen Wenbin,Gong Picheng,Jiang Cuishuang,Gao Changfei,Yang Bing,Yang Chenyu 한국탄소학회 2023 Carbon Letters Vol.33 No.3

In this study, a bipolar visible light responsive photocatalytic fuel cell (PFC) was constructed by loading a Z-scheme g-C3N4/carbon black/BiOBr and a Ti3C2/MoS2 Schottky heterojunction on the carbon brush to prepare the photoanode and photocathode, respectively. It greatly improved the electron transfer and achieved efficient degradation of organic pollutants such as antibiotics and dyes simultaneously in two chambers of the PFC system. The Z-scheme g-C3N4/carbon black/BiOBr formed by adding highly conductive carbon black to g-C3N4/BiOBr not only effectively separates the photogenerated carriers, but also simultaneously retains the high reduction of the conduction band of g-C3N4 and the high oxidation of the valence band of BiOBr, improving the photocatalytic performance. The exceptional performance of Ti3C2/MoS2 Schottky heterojunction originated from the superior electrical conductivity of Ti3C2 MXene, which facilitated the separation of photogenerated electron–hole pairs. Meanwhile, the synergistic effect of the two photoelectrodes further improved the photocatalytic performance of the PFC system, with degradation rates of 90.9% and 99.9% for 50 mg L−1 tetracycline hydrochloride (TCH) and 50 mg L−1 rhodamine-B (RhB), respectively, within 180 min. In addition, it was found that the PFC also exhibited excellent pollutant degradation rates under dark conditions (79.7%, TCH and 97.9%, RhB). This novel pollutant degradation system is expected to provide a new idea for efficient degradation of multiple pollutant simultaneously even in the dark.

Osteogenic effect of magnesium oxychloride cement modified with phytic acid and loaded with strontium ranelate

Tingting Ma,Yijia Guan,Jinlun Feng,Yue Yang,Junying Chen,Wenjie Guo,Jianguo Liao,Yanru Zhang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Magnesium oxychloride cement has good mechanical properties, but poor water resistance. Methods Phytic acid, which can form chelate with Mg2+, was used to modify magnesium oxychloride cement, and the effects of phytic acid on the strength, in vitro degradation and biological activity of magnesium oxychloride cement were studied. Based on the preparation of phytic acid modified magnesium oxychloride cement with good water resistance and biological activity, osteoporosis treatment strontium ranelate was loaded on phytic acidmagnesium oxychloride cement, strontium ranelate/phytic acid-magnesium oxychloride cement was prepared. Results It was found that the compressive strength of 1.25 wt% phytic acid-magnesium oxychloride cement after soaking in SBF for 28 d could reach 40.5 ± 2.0 MPa, 13.33% higher than that of the control group (when phytic acid was 0 wt%), and the mass loss rate of all ages was lower than that of the control group. The water resistance of magnesium oxychloride cement was effectively improved by phytic acid. After loading with strontium ranelate, the water resistance of 1.25 wt% phytic acid-magnesium oxychloride cement was improved. Cell experiments showed that strontium ranelate could effectively promote cell proliferation and improve the expression of osteoblastrelated proteins. When strontium ranelate/phytic acid-magnesium oxychloride cement samples were implanted subcutaneously in rats for 4 w, no obvious inflammatory response was observed, and the material was tightly bound to the surrounding tissues. When bone cement was implanted into rat femur for 4 w, the bone cement was gradually wrapped and absorbed by new bone tissue, which grew from the outside to the inside, indicating that the bone cement containing strontium ranelate/phytic acid-magnesium oxychloride cement had excellent bone-forming ability. Conclusions In conclusion, the results indicated that strontium ranelate/phytic acid-magnesium oxychloride cement composite bone cement had a potential application prospect in clinical bone repair.

Fully Distributed Economic Dispatching Methods Based on Alternating Direction Multiplier Method

Linfeng Yang,Tingting Zhang,Guo Chen,Zhenrong Zhang,Jiangyao Luo,Shanshan Pan 대한전기학회 2018 Journal of Electrical Engineering & Technology Vol.13 No.5

Based on the requirements and characteristics of multi-zone autonomous decision-making in modern power system, fully distributed computing methods are needed to optimize the economic dispatch (ED) problem coordination of multi-regional power system on the basis of constructing decomposition and interaction mechanism. In this paper, four fully distributed methods based on alternating direction method of multipliers (ADMM) are used for solving the ED problem in distributed manner. By duplicating variables, the 2-block classical ADMM can be directly used to solve ED problem fully distributed. The second method is employing ADMM to solve the dual problem of ED in fully distributed manner. N-block methods based on ADMM including Alternating Direction Method with Gaussian back substitution (ADM_G) and Exchange ADMM (E_ADMM) are employed also. These two methods all can solve ED problem in distributed manner. However, the former one cannot be carried out in parallel. In this paper, four fully distributed methods solve the ED problem in distributed collaborative manner. And we also discussed the difference of four algorithms from the aspects of algorithm convergence, calculation speed and parameter change. Some simulation results are reported to test the performance of these distributed algorithms in serial and parallel.

Microstructural and Textural Differences Induced by Water and Furnace Cooling in Commercially Pure Zr Annealed in the α + β Region

Linjiang Chai,Tingting Wang,Yi Ren,Bo Song,Ning Guo,Liangyu Chen 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.4

In this work, a commercially pure Zr sheet with a typical bimodal basal texture was annealed in an α + β region and thensubjected to Different coolings (in water and furnace). Microstructures and textures of both the as-received and the heat-treatedspecimens were investigated by electron channeling contrast imaging and electron backscatter diff raction techniques. Resultsshow that a duplex microstructure consisting of untransformed bulk α grains and twinned martensitic plates is produced inthe water-cooled specimen, which possesses a weakened texture compared to the initial one. For the specimen cooled in furnace,however, a uniform microstructure fully comprised of coarser equiaxed grains with a strengthened texture is obtained. Analyses reveal that the rapid cooling in water could suppress variant selection behaviors during β → α transformation andallow α plates with scattered orientations to be nucleated inside β phases, contributing to the weakened texture. In contrast,during slow cooling in furnace, β boundaries would act as preferred nucleation sites of α embryos, resulting in a strong variantselection that accounts for the intensifi ed texture.

Growth Inhibitory and Pro-Apoptotic Effects of Hirsuteine in Chronic Myeloid Leukemia Cells through Targeting Sphingosine Kinase 1

Gao Shan,Guo Tingting,Luo Shuyu,Zhang Yan,Ren Zehao,Lang Xiaona,Hu Gaoyong,Zuo Duo,Jia Wenqing,Kong Dexin,Yu Haiyang,Qiu Yuling 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.6

Chronic myeloid leukemia (CML) is a slowly progressing hematopoietic cell disorder. Sphingosine kinase 1 (SPHK1) plays established roles in tumor initiation, progression, and chemotherapy resistance in a wide range of cancers, including leukemia. However, small-molecule inhibitors targeting SPHK1 in CML still need to be developed. This study revealed the role of SPHK1 in CML and investigated the potential anti-leukemic activity of hirsuteine (HST), an indole alkaloid obtained from the oriental plant Uncaria rhynchophylla, in CML cells. These results suggest that SPHK1 is highly expressed in CML cells and that overexpression of SPHK1 represents poor clinical outcomes in CML patients. HST exposure led to G2/M phase arrest, cellular apoptosis, and downregulation of Cyclin B1 and CDC2 and cleavage of Caspase 3 and PARP in CML cells. HST shifted sphingolipid rheostat from sphingosine 1-phosphate (S1P) towards the ceramide coupled with a marked inhibition of SPHK1. Mechanistically, HST significantly blocked SPHK1/S1P/S1PR1 and BCR-ABL/PI3K/Akt pathways. In addition, HST can be docked with residues of SPHK1 and shifts the SPHK1 melting curve, indicating the potential protein-ligand interactions between SPHK1 and HST in both CML cells. SPHK1 overexpression impaired apoptosis and proliferation of CML cells induced by HST alone. These results suggest that HST, which may serve as a novel and specific SPHK1 inhibitor, exerts anti-leukemic activity by inhibiting the SPHK1/S1P/ S1PR1 and BCR-ABL/PI3K/Akt pathways in CML cells, thus conferring HST as a promising anti-leukemic drug for CML therapy in the future.

Fully Distributed Economic Dispatching Methods Based on Alternating Direction Multiplier Method

Yang, Linfeng,Zhang, Tingting,Chen, Guo,Zhang, Zhenrong,Luo, Jiangyao,Pan, Shanshan The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.5

Based on the requirements and characteristics of multi-zone autonomous decision-making in modern power system, fully distributed computing methods are needed to optimize the economic dispatch (ED) problem coordination of multi-regional power system on the basis of constructing decomposition and interaction mechanism. In this paper, four fully distributed methods based on alternating direction method of multipliers (ADMM) are used for solving the ED problem in distributed manner. By duplicating variables, the 2-block classical ADMM can be directly used to solve ED problem fully distributed. The second method is employing ADMM to solve the dual problem of ED in fully distributed manner. N-block methods based on ADMM including Alternating Direction Method with Gaussian back substitution (ADM_G) and Exchange ADMM (E_ADMM) are employed also. These two methods all can solve ED problem in distributed manner. However, the former one cannot be carried out in parallel. In this paper, four fully distributed methods solve the ED problem in distributed collaborative manner. And we also discussed the difference of four algorithms from the aspects of algorithm convergence, calculation speed and parameter change. Some simulation results are reported to test the performance of these distributed algorithms in serial and parallel.

Optimal Allocation Strategy Based on Stackelberg Game for Inspecting Drunk Driving on Traffic Network

( Yingmo Jie ),( Mingchu Li ),( Tingting Tang ),( Cheng Guo ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.12

As the main means to cope with the stubborn problem of drunk driving, the inspection of drunk driving has already been paid more attention and thus reinforced. In this paper, we model this scenario as a Stackelberg game, where the police department (called defender) allocates resources dynamically in terms of the traffic situation on the traffic network to arrest drink drivers and drivers who drink (called attacker), whether choosing drunk driving or designated driving service, expect to minimize their cost for given travel routes. However, with the number of resources are limited, our goal is to calculate the optimal resource allocation strategy for the defender. Therefore, first, we provide an effective approach (named OISDD) to fulfill our goal, i.e., generate the optimal strategy to inspect drunk driving. Second, we apply OISDD to directed graphs (which are abstracted from Dalian traffic network) to analyze and test its correctness and rationality. The experimental results show that OISDD is feasible and efficient.