Carbon tetrachloride를 투여한 rat의 hepatic lipid 축적에 미치는 vitamin E의 효과에 관한 연구

박은주,이경연,이미영,이외숙,장재정,정귀은,최진희 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

The present studies were undertaken to evaluate the effect of vitamin E, CCl_4 on the change of hepatic triglyceride, hepatic cholesterol, hepatic phospholipid in male rat. The result obtained from this study were summarised as follows: 1. Hepatic phospholipid of CCl_4 treated rat was increased in proportion to CCl_4 dosage but after concomitant injection(I.P) of vitamin E and CCl_4, hepatic phospholipid was significantly decreased in comparison to that of CCl_4 alone injection. 2. There was no effect on hepatic cholesterol concentration either CCl_4 alone injection(I.P) or concomitant injecton(I.P) of vitamin E and CCl_4. 3. Hepatic triglyceride of CCl_4-treated rat was significantly increased in comparison to that of normal rat but hepatic triglyceride of rat concomitant injection of vitamin E and CCl_4 was significantly decreased in comparison to that of CCl_4 alone injection.

Isolement et quelques caractéristiques physico-chimiques de la lipoxygénase de son de riz

Gui Ju LEE,J. NICOLAS 啓明大學校 生活科學硏究所 1980 科學論集 Vol.6 No.-

Isolement et quelques caracteristiques physico-chimiques de la lipoxygenase de son de riz

LEE, Gui Ju,NICOLAS, J 啓明大學校 生活科學硏究所 1980 科學論集 Vol.6 No.-

효소제를 첨가한 비지의 발효과정중 탄수화물 성분변화에 관한 연구

이귀주 ( Gui Ju Lee ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.1

This study was aimed far the utilization of carbohydrate in the soybean curd residue by pre-treatment of hydrolytic enzymes and following natural fermentation. The content of carbohydrate of soybean curd residue was 40.2% on the dry basis and that of total dietary fibre was 21.7%. The composition of dietary fibre in cellulose, hemicellulose, and lignin were 53.1, 46.8% and 9.4%, respectively. The amount of glucose was increased through the fermentation and its increase was accelerated by pretreatment of hydrolytic enzymes. This phenomenon was believed that glucose, amino acids and fatty acids produced by hydrolytic enzymes improved the nutritional sources for microorganisms involving in the fermentation of soybean curd residue. However, the amount of starch and cellulose was decreased during the fermentation and the rate of disappearance of starch was faster than that of cellulose. The optimum temperature was 30℃ for the increase of glucose amount and the decrease of starch and cellulose in the soybean curd residue in the fermentation. The results taken together suggest that the soybean curd residue is worth to be utilized after simple treatment of enzymes, and the residues can be added to the Meju preparation.

Original Articles : Effect of aldosterone on the amplification of oncolytic vaccinia virus in human cancer Lines

( Hyun Ju Lee ),( Ja Sung Rho ),( Shao Ran Gui ),( Mi Kyung Kim ),( Yu Kyoung Lee ),( Yeon Sook Lee ),( Jeong Eun Kim ),( Eu Na Cho ),( Mong Cho ),( Tae Ho Hwang ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: JX-594 is an oncolytic virus derived from the Wyeth vaccinia strain that causes replication-dependent cytolysis and antitumor immunity. Starting with a cross-examination of clinical-trial samples from advanced hepatocellular carcinoma patients having high levels of aldosterone and virus amplification in JX-594 treatment, we investigated the association between virus amplification and aldosterone in human cancer cell lines. Methods: Cell proliferation was determined by a cell-counting-kit-based colorimetric assay, and vaccinia virus quantitation was performed by quantitative polymerase chain reaction (qPCR) and a viral plaque assay. Also, the intracellular pH was measured using a pH-sensitive dye. Results: Simultaneous treatment with JX-594 and aldosterone significantly increased viral replication in A2780, PC-3, and HepG2 cell lines, but not in U2OS cell lines. Furthermore, the aldosterone treatment time altered the JX-594 replication according to the cell line. The JX-594 replication peaked after 48 and 24 hours of treatment in PC-3 and HepG2 cells, respectively. qPCR showed that JX-594 entry across the plasma membrane was increased, however, the changes are not significant by the treatment. This was inhibited by treatment with spironolactone (an aldosterone-receptor inhibitor). JX-594 entry was significantly decreased by treatment with EIPA [5-(N-ethyl-N-isopropyl)amiloride; a Na+/H+-exchange inhibitor], but aldosterone significantly restored JX-594 entry even in the presence of EIPA. Intracellular alkalization was observed after aldosterone treatment but was acidified by EIPA treatment. Conclusions: Aldosterone stimulates JX-594 amplification via increased virus entry by affecting the H+ gradient. (Korean J Hepatol 2011;17:213-219)

촉지되지 않는 갑상선 결절의 특성과 악성 결절 예측과의 관련성

이주영 ( Ju Young Lee ),이정은 ( Jung Eun Lee ),문성수 ( Seong Su Moon ),박순홍 ( Soon Hong Park ),이신원 ( Sin Won Lee ),정귀화 ( Gui Hwa Jung ),김정국 ( Jung Guk Kim ),하승우 ( Sung Woo Ha ),김보완 ( Bo Wan Kim ),이재태 ( Jae Ta 대한내과학회 2005 대한내과학회지 Vol.69 No.5

목적 : 최근 초음파 기기의 발달로 촉지 되지 않는 갑상선 결절의 발견 빈도가 늘어나고 있고 이에 따른 초음파 유도하 세침흡인 검사의 빈도도 늘어나고 있으나 이런 우연종의 진단 및 진료에 있어 확립된 기준이 없는 상태이다. 모든 환자에게서 세침흡인 검사를 시행할 수는 없으므로 세침흡인 검사를 시행하기 이전에 악성 결절을 예측하는데 도움이 되는 지표들을 알아보고 악성 가능성에 대해 알아보고자 본 연구를 시행하였다. 방법 : 2001년 7월부터 2003년 12월까지 경북대학교 병원을 내원한 환자 중 초음파 및 컴퓨터 단층 촬영에서 갑상선 결절이 확인되었으나 이학적 검사상 결절이 촉지 되지 않고 병리학적으로 최종 진단을 받은 환자를 대상으로 하였다. 320명을 대상으로 하여 병력지를 통한 후향적 연구조사를 시행하였으며 갑상선 질환 및 방사선 조사의 과거력, 결절 진단 이후 세침 흡인 검사까지의 지속기간, 갑상선 초음파, 갑상선 기능검사, 자가 항체 검사, 핵의학 스캔, 세침 흡인 검사, 수술 소견을 조사하였다. 이러한 세부 항목들의 갑상선 우연종에서의 양상 및 악성을 예측하는데 있어서의 유용성, 악성 결절 진단에 있어 예민도와 특이도를 조사하였다. 결과 : 320명의 대상 환자 중 양성 268명, 악성 52명이었고, 조사 항목 중 초음파 소견에서의 구조, 에코 강도, 석회화, 경계, 모양이 통계학적으로 의미있는 값을 나타냈고 이외에 성별, 연령, 갑상선 기능검사, 자가 항체검사, 핵의학 스캔 등은 악성 결절예측에 도움이 되지 않는 것으로 나타났다. 초음파상 세부 항목의 소견이 같이 존재하는 결절의 경우 민감도와 특이도는 80% 이상의 값을 나타냈다. 결론 : 최근 고해상도 초음파의 도입으로 촉지 되지 않는 갑상선 결절의 빈도가 증가하고 있고 이에 따라 초음파 유도하 세침흡인 검사의 빈도도 증가하고 있다. 세침흡인 검사 시행이전의 초음파상 결절의 구조, 모양, 경계, 석회화 등이 악성 결절 예측에 도움이 될 수 있었고 의미있는 세부 항목이 같이 존재하는 경우는 민감도와 특이도가 증가하여 이러한 결절을 가진 환자에 있어서는 적극적인 조사 및 경과관찰이 필요할 것으로 생각된다. Background : Thyroid nodules are commonly encountered in clinical practice and a p revalence of general population is 19~67% by ultrasonography. We made a study to investigate clinical characteristics, malignancy ratio of thyroid incidentalomas and predicting factors for diagnosis of malignancy, to provide recommendation for evaluation. Methods : We retrospectively evaluated the medical records of 320 patients who visited Kyungpook National University Hospital from July 2001 to December 2003 with non palpable thyroid nodules. We reviewed physical examination, ultrasonographic findings, laboratory findings, histology of thyroid nodules and did statististic analysis to examine relationship to malignancy. Results : 52 patients revealed papillary carcinomas and malignancy rate whin incidentalomas is 9.3%. Sonographic findings of solid structure, hypoechogenecity, punctate calcification, ill defined margin, irregular shape showed meaningful diagnostic value for malignancy. Conclusions : Combination of meaningful sonographic findings increased possibility of maliganancy and require active mangements including recurrent fine needle aspiration and surgery if needed.(Korean J Med 69:474-480, 2005)

미숙아에서 발견된 중복장중첩증 1례

이정주 ( Jung Ju Lee ),김상원 ( Sang Won Kim ),유병훈 ( Byung Hoon Yoo ),차성재 ( Sung Jae Cha ),이종범 ( Jong Beum Lee ),권귀영 ( Gui Young Kwon ) 대한주산의학회 2010 Perinatology Vol.21 No.4

Intussusception is a common surgical disorder in infancy. Intussusception in preterm infant is very rare and about 40 cases have been reported. Furthermore, double intussusception is extremely rare in children and there seems to be no such a case reported in preterm infant. We report a case of idiopathic double intussusception in the preterm infant, who was born at 25 weeks` gestation, underwent an explolaparotomy for bowel perforation on 39 days of life, and during the operation, an ileo-ileo-colic intussusception was found without a leading point.

배양 인체 피부 흑색종세포에 대한 제라늄오일의 항산화 활성 및 멜라닌 합성에 미치는 영향

박승택 ( Seung Taeck Park ),유영월 ( Young Wall Ryu ),김선주 ( Sun Ju Kim ),이귀영 ( Gui Yeong Lee ),정다정 ( Da Jung Jung ),장병수 ( Byung Soo Chang ),범희주 ( Hee Ju Beom ),이화정 ( Hwa Jeong Lee ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2011 대한미용학회지 Vol.7 No.3

For the purpose of searching for whitening resources using essential oil of herb, the antioxidative activity and melanogenesis of Geranium (Palargonium graveolens) oil were examined in cultured human skin melanoma cells (SK-MEL-3). the cell viability, superoxide dismutase (SOD)-like activity, inhibitory activity of lipid peroxidation, tyrosinase activity and total amount of melanin were measured by colorimetric assay. For this experiment, SK-MEL-3 cells were treated with the media containing 25~55 μU/mL, glucose oxidase (GO) for 4 hours, and assessed for the cytotoxicity. GO significantly decreased cell viability compared with control and XTT50 value was determined at 45 μU/mL of GO. In the antioxidative activity of Geranium oil, it showed SOD-like activity and the inhibitory activity of lipid peroxidation. While, in the melanogenesis of Geranium oil, Geranium oil significantly decreased the tyrosinase activity and total amount of melanin which were increased by GO-induced cytotoxicity. From these results, it is suggested that Geranium oil was effective in the protection of GO-induced cytotoxicity by antioxidative activity and inhibitory effect of melanogenesis. Finally, an essential oil such as Geranium oil may be a putative agent for the improvement of whitening effect.

Effect of Cysteine on the Indirubin Production by Recombinant E. coli Harboring fmo Gene

Gui Hwan HAN,Seong Eun BANG,Sun Lee JU,Si Wouk KIM 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.4

품종별 한국산 감자 슬라이스의 냉장 중 Polyphenol oxidase , 페놀함량 및 갈변정도의 변화

이귀주,정현미 한국식생활문화학회 1995 韓國食生活文化學會誌 Vol.10 No.2

Three potato cultivars, Sumi, Daejima and Namjak, were prepared as slices. They were dipped in distilled water for 20 seconds. The potato slices were packed in polyethylene bags and stored at 5℃. Browning degree, total phenol and chlorogenic acid contents and polyphenol oxidase (PPO) activity were measured. And the correlation analysis of browning parameters were conducted. The results were as follows. There were increase in browning degree, phenolic content and PPO activity during cold storage of potato slices with different cultivars. Among three cultivars, Sumi showed the highest browning degree, phenolic content and PPO activity and also showed the highest % increase of browning and PPO activity during cold storage. On the contrary, Daejima was the lowest. But Daejima showed the highest % increase in phenolic contents during cold storage. With Sumi, browning degree was significantly correlated with PPO activity and phenolic contents (p<0.05). With Daejima and Namjak, a significant correlation was found between browning degree and PPO activity (p<0.05). From the above results, enzymatic browning reactions of potato slices and factors affecting them were dependent on cultivar. Among the tested three cultivars, Daejima showed the lowest browning degree during cold storage and thus seems to be desirable for minimal processing.