RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

갑상선 유두암이 병발된 가족성 대장 용종증 1예

백종현,소창배,이은영,이지숙,신은경,박은호,서정아,양재홍,송준영,박선자,박요한,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome, typically characterized by multiple colorectal adenomas and increased incidence of colorectal carcinomas if it is not treated. Moreover, a variety of extracolonic manifestation are seen. The prevalence of thyroid tumors developing in patients with FAP is about 1∼2%. Generally, papillary thyroid cancer indicates low mortality but ,however, as people get older, the cancer becomes more aggressive. So, the operation is required. Recently we experienced a case of FAP, presenting with papillary thyroid carcinoma, and reported with a brief review of literatures.

악골에 발생한 백악질 및 골화성섬유종의 임상 및 방사선학적 고찰

박창서,김기덕,조은영 大韓口腔顎顔面放射線學會 1997 Imaging Science in Dentistry Vol.27 No.2

The purpose of this study was to know the proper diagnosis and to establish the treatment plan of cementifying and ossifying fibroma in the jaws through the clinical, raiological, and histopathologic considerations. The authors compared and analyzed the clinicoradiologic features of the thirteen cases of cementifying and ossifying fibroma, diagnosed at the Dental college hospital in Yonsei university, Seoul, Korea, during the period from 1980 to 1995. The obtained results were as follows: 1. Cementifying and ossifying fibroma occurred in the mean age, 44 years, ranged from 29 to 65 years and the male to female ratio was approximately 1:5. 2. Swelling was the most common frequent presenting complaints. Other reported symptoms included pain, tooth mobility and asymptom. 3. The frequency of the lesions was twelve cases in the mandible and one case in the maxilla. And eleven of thirteen cases were distributed on the premolar and molar region. 4. Radiologically, eight of thirteen cases were well defined lesions, five cases were relatively well defined lesions. And nine of thirteen cases were mixed lesions, three cases were radiopaque lesions, and only one case was purely radiolucenct lesion. 5. Histologically, seven of thirteen cases were classified ossifying fibroma, four cases were cemento-ossifying fibroma, and two cases were cementifying fibroma.

나이에 따른 한국인 정상 이하선의 전산화단층촬영값의 차이

박창서,김기덕,이은숙,정호걸 大韓口腔顎顔面 放射線學會 1999 Imaging Science in Dentistry Vol.29 No.2

Purpose : To determine normal CT number range of parotid gland by analyzing the change by age increase and the difference among individuals and between both sexes in CT number of normal parotid gland. Materials & Methods : 134 subjects who took the CT scan between the period of Jan. 1996 and Dec. 1997 at Yonsei University, Dental Hospital were selected. Criteria for selection were that the patients must be within the normal range clinically and radiologically, and the entire parotid gland on the axial view must be shown. Among the axial views, the one showing the greatest parotid gland size was selected and its CT number was recorded. Also, CT numbers from both masseter muscle were recorded as its control. Results : There was statistically significant correlation between CT number of right and left of parotid glands and masseter muscles. With the increase of age, there is a significant decrease in the CT number of parotid gland(p<0.05). There was no statistically significant difference in CT number between sexes except the 7th decade and beyond age group of parotid gland(p>0.05). Conclusion : As age increases, CT number of parotid gland has a tendency to decrease, and there is no significant difference in the CT numbers between left and right parotid gland. Therefore in the CT scan of patients suspected of having a salivary gland disease of the parotid gland, to consider normal range of the age-dependent CT numbers of parotid gland and compare the CT numbers of the right and left parotid gland might be useful in diagnosing the disease.(J Korean Oral Maxillofac Radiol 1999;29:451-458)

상악동 병변의 인지시 파노라마 방사선사진의 유용성

박창서,이은숙 大韓口腔顎顔面 放射線學會 1999 Imaging Science in Dentistry Vol.29 No.1

Purpose: to evaluate the usefulness of the panoramic radiography, generally used in dental clinic, for the detection of the maxillary sinus pathosis due to the inflammation and tumor, through the comparison with CT findings as the absolute standard. Materials & Methods: 150 maxillary sinuses of 75 patients with or without particular signs and symptoms and showing soft tissue lesions or destruction of maxillary sinus walls in at least one sinus in CT, were selected as subject samples, and the panoramic radiography of the same patients were interpretated by 3 dental radiologists and 3 non dental radiologist given no previous informations. Using the CT findings as the absolute standard, the diagnostic ability of panoramic radiography in the mucosal thickening, maxillary sinus haziness, inferior wall destruction, medial wall destruction, posterolateral wall destruction and the superior wall destruction was evaluated using the ROC curve and the difference between dental radiologist group and non dental radiologist group was also evaluated. Results: 1. When dental radiologist group interpretated the destruction of inferior wall and posterolateral wall, the kappa value which shows interobserver's coincidence was above 0.75. 2. The diagnostic ability according to site of interpretation was the highest when the inferior wall was interpretated in both observer groups and there was a statistically significant difference between the dental radiologist group and non dental radiologist group in interpretating the mucosal thickening, haziness, destruction of the inferior and medial wall(p<0.05). 3. The diagnostic ability in detecting the destruction of the sinus walls was better than in soft tissue lesions in both groups and between the groups there was a statistically significant difference(p<0.05). Conclusion: When detecting the destruction of inferior and posterolateral wall of the maxillary sinus there was coincident with that of CT findings, and so it is considered that diagnostic ability of panoramic radiography is high in this point. But in interpretating the destruction of medial wall and soft tissue lesions, diagnostic ability of the panoramic radiography was relatively low and there was a significant difference between the dental radiologist group and non dental radiologist group. Therefore CT or other auxiliary diagnostic method will be necessary when evaluating thorough pathosis of maxillary sinus objectively.(J Korean Oral Maxillofac Radiol 1999:29:221-237)

위장관의 원발성 B 세포 악성 림프종의 재분류와 bcl-2, cyclin D1, bcl-6 및 p53 발현의 의미

서정균,김태원,김병수,조은택,박찬국,김만우,이미자,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : In recent years, the REAL (Revised European American Lymphoma) classification provided a new category of MALT (mucosa-associated lymphoid tissue) lymphoma and mantle cell lymphoma in B cell lymphomas. We have analyzed the expression of bcl-2, cyclin D1, bcl-6 and p53 and correlated with the subtypes and histologic grade of primary gastrointestinal B cell lymphoma. Also we investigated the usefulness of immunophenotypic features in diagnosis of low grade B cell lymphoma. Materials and Methods : Twenty-two cases of primary gastrointestinal B cell lymphoma were recategorized in low grade MALT lymphoma, low/high grade MALT lymphoma, high grade MALT lymphoma, and diffuse large cell lymphoma according to the morphological findings. We investigated the expression of bcl-2, cyclin D1, bcl-6 and p53 by immunohistochemical method. Results : The bcl-2 protein expression was higher in the low grade MALT lymphoma than in the high grade lymphoma. The cyclin D1 protein expression was negative in all cases. The expression of bcl-6 and p53 protein was negative in all low grade MALT lymphoma. Conclusion : The results suggest that we can differentiate the low grade lymphoma from the high grade lymphoma by immunohistochemical staining for bcl-2, bcl-6, and p53 protien.

충남 일부지역 초등학생의 인두에 분포하는 세균 실태조사

하서영,박수진,이보미,박솔기,박창은 남서울대학교 2012 남서울대학교 논문집 Vol.18 No.2

The distributed species and the kinds of variable microorganism in Pharynx were monitored to the elementary school child. Our purpose was to investigate the distribution of bacteria in Pharynx of child except for adults. Analysis performed the collected sample from 15 students in Chungnam area for 2 days from July 30 to July 31 in 2012. The isolated bacteria were identified by Gram stain and biochemical test using VITEKⅡsystems. The experiment on microorganism concentration of contact parts carried out and the average of total microorganism was measured. The elementary child's Pharynx were isolated and identified. The isolation rates were major distributied to Gram positive cocci, another Gram negative bacilli and Gram positive bacilli. respectively. The frequently isolated bacteria were G. adiacens. The Granulicatella adiacens is one of the fastidious Gram positive cocci previously described as nutritionally variant streptococci due to their requirement of L-cysteine, pyridoxal, or thiol compounds for growth. These bacteria have been identified as significant causative agents of endocarditis, opthalmic infections, and meningitis. Further systematic studies are necessary with an emphasis on species identification. Key Words : Microorganism, Elementary child, Pharynx and Larynx

아동학대의 발생 현황과 관련변인에 대한 연구

서은주,박혜인 啓明大學校 生活科學硏究所 1994 科學論集 Vol.20 No.-

This study was attempt to survey the recent trends of child abuse and to explore the related variables. The subjects were 436 children in the fifth and sixth grade of the elementary school in Taegu and 436 their parents by coupling-data. The main results of this study are as follows: 1. Significant variables related to the child were child's sex and friendship. And significant variables related to the parents were parental unemployment, child rearing attitudes, violence experience, life satisfaction. Then significant variables related to the home environment were family type, residential style, harmony of the home and martal violence. 2. The most significant variable in all type of child abuse was the variable related to the parents. Additionally marital violence was the most influential variable in all types of child abuse.

韓國産 紅花子와 中國産 紅花子가 鎭痛 , 消炎 및 關節炎에 미치는 效果

서부일,이은숙,박지하,김상찬,변부형,최호영 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

This experimental study was done to compare the efficacy of Korean and Chinese Carthami Semen on analgesic effect and remedy of inflammation and arthritis. The results were as follows; 1. In analgesic action on the writhing syndrome in mice induced by 0.7% acetic acid, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in analgesic action on the writhing syndrome in mice induced by Acetylcholine HC1, Korean and chinese Carthami Semen extract group showed significant decrease in comparison with control group. 2. In Anti-inflammatory effects, Chinese Carthami Semen extract group showed significant decrease at 150 and 180 minutes after inducing paw-edema in comparison with control group. 3. In the method of adjuvant arthritis, orally administered for 21 days, Korean Carthami Semen extract group showed significant inhibition of the hind paw edema after 1 day and 21 days. Whereas Chinese Carthami Semen extract group showed some reduction, but the results didn's show significant decrease. 4. In the level of leucocytes count, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in the level of total protein. CRP and glucose, Korean and Chinese Carthami Semen extract group didn't showed significant change in comparison with control group.

표면탄성파를 이용한 프라즈마 폴리머박막의 습기감지특성 측정

박남천,서은덕 경남대학교 신소재연구소 1992 論文集 Vol.2 No.-

습도의 측정은 과학과 공학의 여러분야에서 중요하다. 표면탄성파를 이용한 습도 센서는 기존의 저항 및 커패시턴스 측정방식의 습도센서보다 감도가 매우 높다. 표면 탄성과 습도센서는 표면탄성과 지연선 위에 습기를 감지할 수 있는 막을 만들고, 이를 이용한 표면탄성과 발진기를 이용한다. 이 막이 습기를 흡수하면 이 막의 전기적 및 기계적 성질이 변화되며, 이는 표면탄성과 발진기의 주파수를 변화시킨다. 본 논문에서는 YZ LiNbO₃기판 위에 헥사풀루오르프로펜박막을 제조하며 이를 이용한 55MHz 표면탄성과 발진기를 만들어서 이 박막의 습기감지특성을 측정하였다. 그 결과 온도 26℃에서 습도가 25%RH에서 85%RH까지 변화할 때 약 9KHz의 발지주파수 이동이 있었으며 온도가 낮아질수록 이 이동이 감소하였다. Measurment and control of humidity play an important role in many branches of science and engineering. Two types of humidity sensors have been intensively studied. These are the capacitive type and the resistive type. The humidity sensor using surface acoustic wave (SAW) have higher sensivity and wide dynamic range than the capactive type and the resistive type sensors. The SAW humidity sensors use SAW delay-line oscillator and the delay-line, being coated with a thin hygroscopic film. Adsorption of moisture produces rapid changes in the electrical and mechanical properties of the film. Hence this results a change in the frequency of the SAW osoillator. The frequency of 55MHz oscillator fabricated on a YZ LiNbO₃substrate with hexafluoropropene thin film is found to vary 9KHz as the relative humidity is varied from 25%RH to 85%RH at 26 C.