간세포암과 감별이 어려웠던 악성중피종 치험 1예

김선문,허원석,채경훈,강윤세,정재훈,김연수,박기오,문희석,이엄석,김석현,성재규,이병석,이헌영,신경숙,조준식,송인상,강대영 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Malignant peritoneal mesothelioma is a rare neoplasm that arises from the mesothelium of a serosal cavity and is a rapidly fatal disease with a median survival of 4 to 12 months for untreated cases. Recently, we experienced a case with malignant peritoneal mesothelioma who was suspected hepatocelluar carcioma by abdominal CT scan and was confirmed by biopsy including immunohistochemical stain(calretinin) after surgery. We performed tumor excisions and wedge resection of the liver(segment Ⅷ)and inserted Tencoff catheter in abdominal cavity at 25th day of post-operation. We treated with intraperitoneal paclitaxel(25mg/m^(2)/day for 5 days) six courses monthly. She was well tolerable and is still living without any evidence of recurrence for 14th month of post-operation.

자가 면역성 간염에서 자가항체의 발현 양상과 스테로이드 치료의 효과

박기오,채경훈,허원석,강윤세,정재훈,김연수,문희석,이엄석,김선문,이병석,김남재,이헌영 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

목적 : 최근 8년간 본원에서 경험한 자기면역성 간염의 혈액학적 특징과 스테로이드의 치료효과를 알아보고자 본 연구를 시행하였다. 방법 : 1996년 이후부터 2002년까지 충남대학교 병원에서 1999년 International Autoimmune Hepatitis Group(LAHG)에서 제시한 수정 진단 기준안에 의거하여 총 진단점수가 probable 이상에 해당하는 환자들을 대상으로 임상적 특징, 검사실 및 조직 소견과 스테로이드와 azathioprine의 치료 효과를 후향적으로 조사하였다. 결과 : 9예의 환자들은 여성이 78%, 40대가 많았고, 급성 발병이 33.3%, 피로감, 식욕부진 및 황달 등이 나타났으며, 항핵항체가 55.6%에서 그리고 항평활근 항체가 77.8%에서 양성이었고, 동반 질환은 류마치스 관절염이 2예, 특발성 혈소판 감소증 1예, Henoch-Sch nlein 자반증 및 그레브스병이 각각 1예 씩이었다. 7예에 대해서 스테로이드와 azathioprine으로 치료하여 생화학적 임상적 완해가 이루어져서 유효한 치료 결과를 얻었다. 결론 : 자가면역성 간염은 우리 나라에서 원인을 찾을 수 없는 만성 간질환의 경우, 특히 중년여성에서 다른 자가면역성 질환이 동반되었을 때 자가면역성 간염을 의심하여 자가항체 선별검사를 시행해 보아야 할 것으로 생각된다. 향후 다기관 연구를 통하여 좀 더 많은 증례를 모으고 전향적인 연구를 시행하여야 할 필요가 있다. Although autoimmune hepatitis(AIH) is common in western contries, some studties have been reported in Korea. The aim of the study was to analyze the clinical and histological features and expression pattern of the autoantibody and to examine clinical course of AIH in Korea. For 9 patients diagnosed as having AIH in our hospital since 1996, we reviewed medical records and analyzed clinical and laboratory findings retrospectively. They had a probable or definite AIH according to the revised criteria of International AIH group. The mean age was 42.7(21∼68)years and 7(77.8%) were female. Three of them(33.3%) showed acute hepatitis and five of them showed cirrhosis. Fatigue and anorexia were the most frequently complained symptoms(55.6%). Four patients(44.4%) were accompanied with other autoimmune diseases. Antinuclear antibody was detected in 5(55.6%) and anti-smooth muscle antibody was detected in 7(77.8%). Three patients showed focal picemeal necrosis and four showed periportal fibrosis. Among 7 patients who received immunosuppressive therapy, six patients(85.7%) showed initial response. and biochemical and serologic remission were achived in 5(71.4%) and 3(42.8%) patients, AIH seems to be partly responsible for chronic liver disease of unknown etiology in Korea. AIH should be suspected in female patients with unknown chronic liver disease of unknown etiolgy, especially accompanied with other autoimmune disease. The clinical characteristics of AIH may not be quite different from those of Western countries.

駝鳥産業의 流通情報와 經營戰略에 관한 硏究

조규석,김계웅:김석은 공주대학교산업개발연구소 2000 産業開發硏究 Vol.8 No.-

This research was undertaken in order to obtain the technological skill and good information on the feeding and raising of ostrich in Korea. Ostrich meat have been confirmed with edible meat in Korea since May, 2000. Ostrich meat contains reddish color, which is low calorie, high protein, and low fat. Most of people preferred to high protein meat contained plenty of nutrients except high fat including high cholesterol. We could utilize as edible and palatable foods such as pure meat, large egg, bone including bone extraction, and fur. Therefore, this animals could be called as an economical poultry. Ostrich husbandry, in the future, should be maintained with environmental agriculture. Without our desirable environment, we as well as animals also could eventually be destruction. The characteristics of raising ostrich as well as duck is the use of remains of extra food as a recycling. Especially, ostrich eats very well voraciously everything to be edible. Especially, ostrich, in poultry, grows very well in early stage in course of growth. On the other hand, it is difficult for most of farmer to market and to improve the strategy of high qualities of meat. Accordingly, many farmers who are raising ostrich try to improve and to enhance feeding skill as well as reproductive and genetic breeding. And then, we should establish logical and systematical marketing strategy through good management including improvement of meat.

TNF-α 유전자형과 방광암과의 관계

정필두,김은정,엄민식,서정원,윤석중,김종석,이상철,김원재 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: TNF-α는 일부 종양의 종양화 과정과 관련이 있는 것으로 알려져 있다. 본 연구는 TNF-α 발현에 영향을 미치는 TNF-α 촉진자 -308 부위의 유전적 다형성이 방광암과 관련이 있는지 유무를 알고자 시행하였다. 대상 및 방법: 유전자 분석을 위하여 환자 113명 및 대조군 109명으로부터 혈액을 채취하여 genomic DNA를 분리한 후 PCR-RFLP 및 direct DNA sequencing을 통하여 TNF-α유전자의 다형성을 조사하여 방광암의 발생, 병기 및 분화도와 비교 검토하였다. 결과: TNF-α 촉진자 -308 부위의 유전형은 대조군에서는 GG형이 83.5%(90례 및 GA형이 16.5%(19례)로 관찰되었으며 AA형은 없었다. 환자군에서는 GG 형이 85.4%(97례), GA형 및 AA형은 각각 13.1%(15례)및 0.8%(1례)에서 관찰되었다. 두 군 모두에서 GG형이 가장 많이 나타났으며 다음으로 GA형을 보이고 AA형은 1례의 방광암 환자에서만 관찰되었다. -308부위의 경우도 두 군 사이에 유전자형의 차이는 없었다(p=0.259) 분화도별 분포를 보면 grade I이 20례, grade II가 49례, grade Ⅲ은 34례였고 병기별로 표재성인 경우가 90례였으며 침윤성은 14례였다. 분화도가 나빠질수록 GA형이 증가하였다(p=0.04). 그러나 병기와 TNF-α promoter -308부위의 유전자형 사이에는 유의한 상관 관계가 없었다(p=0.123). 결론: 방광암 환자의 혈액에서 GA genotype이 관찰되는 경우, 분화도가 나쁠 가능성이 매우 높기 때문에 좀 더 적극적인 치료와 세밀한 추적관찰을 함으로써 방광암으로 인한 사망과 암의 진행을 예방할 수 있을 것으로 기대한다. Purpose : Tumor necrosis factor-alpha (TNF-α) is involved in tumorigenesis of several cancers as an endogenous tumor promoter. The purpose of this study was to investigate whether genetic polymorphism of TNF-α promoter region (-308) was associated with human bladder tumor. Materials and Methods: The DNA from 113 and 109 respective blood samples of bladder tumor Patients and control group was analyzed by PCR-based restriction fragment length polymorphism (RFLP) and direct DNA sequencing methods to characterize the genetic polymorphism of -308 promoter region of the TNF-α gene in bladder tumor patients. We compared the association of bladder tumor with genetic Polymorphism of TNF-α promoter region(-308) in relation to the stage, grade, recurrence and progressio. Results : Eighty-six percents(97/113) of bladder tumor patients and 83.5% (90/109) of control group showed genotype GG at -308 region of TNF-α. Difference in genetic variations of TNF-α promoter (-308) did not exist between bladder tumor patients and control group(p=0.259). Tumor grade was significantly related to the GA genotype (p=0.04). The higher is the grade in bladder tumor, the more frequent was the GA genotype. Tumor stage, recurrence and progression were not significantly associated with genetic polymorphism of TNF-α promoter region (-308). Conclusion: The GA genotype of TNF-a promoter region (-308) had a significant impact on TNF-α production and was related to higher grade tumor compared to GG genotype. TNF-α serum levels in bladder tumor patients were significantly higher than controls. These data suggested that TNF-α might involve the tumorigenesis of the bladder rather than treatment or prevention of bladder tumor.

Neutrophils Are the Predominant Infected Phagocytic Cells in the Airways of Patients With Active Pulmonary TB

Eum, Seok-Yong,Kong, Ji-Hye,Hong, Min-Sun,Lee, Ye-Jin,Kim, Jin-Hee,Hwang, Soo-Hee,Cho, Sang-Nae,Via, Laura E.,Barry III, Clifton E. Elsevier 2010 Chest Vol.137 No.1

A Rapid Assessing Method of Drug Susceptibility Using Flow Cytometry for Mycobacterium tuberculosis Isolates Resistant to Isoniazid, Rifampin, and Ethambutol

Eum Seok-Yong,Baek Seung-Hun,Hong Min-Sun,Lee Jong-Seok,Cho Eun-Jin,Lee Ji-Im,Cho Sang-Nae,Sun-Kyoung Lee 대한결핵및호흡기학회 2022 Tuberculosis and Respiratory Diseases Vol.85 No.3

Background: The current conventional drug susceptibility test (DST) for <i>Mycobacterium tuberculosis</i> (Mtb) takes several weeks of incubation to obtain results. As a rapid method, molecular DST requires only a few days to get the results but does not fully cover the phenotypic resistance. A new rapid method based on the ability of viable Mtb bacilli to hydrolyze fluorescein diacetate to free fluorescein with detection of fluorescent mycobacteria by flow cytometric analysis, was recently developed.Methods: To evaluate this cytometric method, we tested 39 clinical isolates which were susceptible or resistant to isoniazid (INH) or rifampin (RIF), or ethambutol (EMB) by phenotypic or molecular DST methods and compared the results.Results: The susceptibility was determined by measuring the viability rate of Mtb and all the isolates which were tested with INH, RIF, and EMB showed susceptibility results concordant with those by the phenotypic solid and liquid media methods. The isolates having no mutations in the molecular DST but resistance in the conventional phenotypic DST were also resistant in this cytometric method. These results suggest that the flow cytometric DST method is faster than conventional agar phenotypic DST and may complement the results of molecular DST.Conclusion: In conclusion, the cytometric method could provide quick and more accurate information that would help clinicians to choose more effective drugs.

Optimization simulation anlaysis of local energy supply based on renewable energy

Eun Seok Eum(음은석),Yong Beum Yoon(윤용범) 대한전기학회 2021 대한전기학회 학술대회 논문집 Vol.2021 No.11

In response to global environmental issues, the IEA announced Net Zero 2050 and the Korean government announced the Carbon Neutrality Declaration 2050. For now, it is expected that our expectations for the future development of the renewable energy industry will increase. Not only isolated regions, but also regions connected to the entire power grid are faced with the need to utilize renewable power generation facilities as an important axis of the power generation system. In this paper, the possibility of utilizing a distributed power system of renewable energy optimized for local energy supply connected to the power grid through HOMER, a microgrid design program, is reviewed.

비상구 유도로봇 제작

조은석(Eum-Seok Cho),권종수(Jong-Soo Kwon),변상준(Sand-Zoon Byun) 한국정보기술학회 2017 Proceedings of KIIT Conference Vol.2017 No.6

포아송 회귀모형과 음이항 회귀모형을 이용한 회전교차로 내 교통사고 예측모형 개발

석한음(Seok, Han Eum),최재헌(Choi, Jae Heon),손영태(Son, Young Tae),김영준(Kim, Young Jun) 대한교통학회 2016 대한교통학회 학술대회지 Vol.75 No.-

국내에서는 2010년을 기점으로 전국적으로 회전교차로 시범사업이 실시되어 설치 및 운영되고 있으나 회전교차로에서의 사고분석에 대한 연구가 아직은 부족한 상황이다. 본 연구에서는 회전교차로의 기하구조와 교통사고 자료를 분석하여 회전교차로에서 발생하는 교통사고의 원인을 파악하고 궁극적으로 사고 발생건수와 그 정도를 줄이고자 하는데 목적이 있다. 이를 위해 2010년 회전교차로 시범사업에서 선정된 회전교차로 중 현장조사 자료가 있는 16개의 회전교차로를 대상으로 포아송 회귀모형과 음이항 회귀모형을 이용하여 분석하였다. 분석결과, 일평균교통량, 중앙교통섬의 지름, 도시/지방부, 회전교차로의 유형의 변수들이 회전교차로 내 교통사고의 모형을 가장 잘 설명할 수 있을 것이라고 판단되었다. 하지만 우리나라 운전자들에게는 아직 회전교차로가 익숙하지 않아 통과방식에 미숙한 운전자들이 많을 것으로 판단하여 통행방법 안내표지판 변수를 추가하였다. 최종적으로 이 5개의 변수로 모형을 구축하였으며, 과산포가 존재하여 음이항 회귀모형을 이용하여 구축하였다.

Essential Engagement of Toll-Like Receptor 2 in Initiation of Early Protective Th1 Response against Rough Variants of <i>Mycobacterium abscessus</i>

Kim, Jong-Seok,Kang, Min-Jung,Kim, Woo Sik,Han, Seung Jung,Kim, Hong Min,Kim, Ho Won,Kwon, Kee Woong,Kim, So Jeong,Cha, Seung Bin,Eum, Seok-Yong,Koh, Won-Jung,Cho, Sang-Nae,Park, Jong-Hwan,Shin, Sung American Society for Microbiology 2015 Infection and immunity Vol.83 No.4

<P>Although <I>Mycobacterium abscessus</I> (<I>M. abscessus</I>) is becoming more prevalent in patients without overt immunodeficiency, little is known about the factors that contribute to disease susceptibility. This study was undertaken to investigate how Toll-like receptor 2 (TLR2) functionally contributes to the generation of protective immunity against <I>M. abscessus</I> in a morphotype-specific manner. We found that <I>Tlr2</I><SUP>−/−</SUP> mice were extremely susceptible to an intravenous (i.v.) model of infection by <I>M. abscessus</I> rough variants, displaying uncontrolled infection in the lungs and a significantly lower survival rate than with wild-type (WT) mice. This uncontrolled infection resulted from failures in the following processes: (i) production of the crucial cytokines gamma interferon (IFN-γ), tumor necrosis factor alpha (TNF-α), and interleukin 12p70 (IL-12p70); (ii) early infiltration of neutrophils, monocytes, and dendritic cells (DCs) in the lungs of <I>Tlr2</I><SUP>−/−</SUP> mice; (iii) rapid influx of CD4<SUP>+</SUP> and CD8<SUP>+</SUP> T cells; and (iv) the expansion of memory/effector T cells. Notably, systemic administration of <I>M. abscessus</I> culture filtrate-treated syngeneic DCs from WT mice greatly strengthened immune priming <I>in vivo</I>, resulting in a dramatic reduction in bacterial growth and improved long-term survival in <I>Tlr2</I><SUP>−/−</SUP> mice, with a recovery of protective immunity. Our findings demonstrate that TLR2 is an essential contributor to instructive and effector immunity during <I>M. abscessus</I> infection in a morphotype-specific manner.</P>