Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

안트라사이트 여과층의 역세척에서 초기 유동화에 관한 연구

이재복,임성진,김의재 慶星大學校 環境問題硏究所 1998 環境硏報 Vol.8 No.1

Filtration is an essential unit process which are used not only to supply clean water but also to treat wastewater by physicochemical methods. Espoecially, the importance of granular filters is growing during the last two decades. While the filtration goes on, the medias. Especially, the importance of granular filters is growing during the last two decades. While the filtration goes on, the media removes suspended solids in the pore or on the surface of it, and then increased the pressure loss of the filter media are increasing, The bockwashing process is the most widely used method in which the washwater flows with high velocity. The objective of this study is to examine existing theoretical equation in relation to the washing efficiency of backwashing in multi-layer filter. And the bench scale experiment was performed for the comparison of the empirical and theoretical studies. By the results of the experimental data, the minimum fluidization velocity is 69.4m/hr and the porosity of the anthracite bed is 0.2043 in incipient fluidized stage, and it is investigated the property of various theoretical equation using in the minimum fluidized stage, and it is investigated the property of various theoretical equation using in the minimum fluidizing stage. After obtained the minimum fluidizing velocity from the experimental data, minimum fluidized velocity replaced 0.4 and was defined as the Ergun's equation for coarse particle than applied equation of the Kozeny-Carman for fine particles. The fluidization of the column begin at 1 which is the ratio of fluidzing velocity and pressure loss. When the superficial velocity is about 4, the fluidization comes from appearance of the minimum fluidization and it showed to reach 11.8 of the maximum pressure loss through fluidization. Until the incipient fluidization displayed, the pressure drop and the superficial fluidizing velocity was found linear as fluidizing velocity increased. The experimental result affirms the correspondency to Darcy's law which describes the flow of fluid at porous media.

압력 저속 냉동 방법의 쥐 치아 치주인대세포 보존 효율 평가

이영은,김의성,김진,한승훈,이승종 大韓齒科保存學會 2009 Restorative Dentistry & Endodontics Vol.34 No.4

본 연구의 목적은 흰 쥐의 상악 대구치를 발거한 후 치주인대세포를 압력을 이용한 저속 냉동 보관법으로 냉동 보관시 치주인대세포의 활성도를 저속 냉동법, 냉장 보관법과 비교하여 평가하고자 하였다. 생후 4주된 암컷 Sprague-Dawley계 흰쥐의 상악 좌우제 1, 2 대구치를 발거하여 각 군 당 18개의 쥐 치아를 MTT, WST-1 검색에 이용하였다. 실험군은 4개군으로 대조군은 즉시 발치군이며, 4℃ 냉장고에 일주일간 보관한 냉장군, 발치 후 동해방지제 처리과정을 거쳐 4℃에서 -35℃까지 -0.5℃/min 속도로 서서히 냉동시킨 뒤 -196℃에 냉동한 저속냉동군 3MPa의 압력을 가하고 -0.5℃/min 속도로 4℃에서 -35℃까지 서서히 냉동시킨 뒤 -196℃에 냉동한 압력 저속 냉동군으로 나누었다. 보존액은 F medium을 사용했으며 동해방지제로 10% dimethylsulfoxide(DMSO)를 사용하였다. 일주일 후 해동하여 MTT, WST-1 검색을 시행한 뒤 치근면을 단위면적으로 표준화하기 위해 MTT, WST-1 측정값을 Eosin 염색 후 530nm에서 측정한 흡광도 값으로 나누었다. 통계 분석을 위해 one way ANOVA를 시행하였으며 사후 검정으로는 Tukey 방법을 사용하였고 결과는 다음과 같다. 1. MTT 검색에 의한 흡광도를 Eosin 염색 후 측정한 흡광도로 나눈 값에서는 압력 저속 냉동군은 즉시 발치군보다 유의성 있게 세포 활성도가 낮았으나(p<0.05), 저속 냉동군이나 냉장군과 비교할 때는 높은 세포 활성도를 보이며 통계적 유의성이 있었다(p<0,05). 2. WST-1검색에 의한 흡광도를 Eosin 염색 후 측정한 흡광도로 나눈 값에서도 MTT 검색과 마찬가지로 압력 저속냉동군에서는 즉시 발치군보다 유의성 있게 세포 활성도가 낮았으나(p<0.05), 저속 냉동군이나 냉장군과 비교할 때는 높은 세포 활성도를 보이며 통계적 유의성이 있었다(p<0.05). 위의 결과를 통해, 3MPa(30기압) 압력을 이용한 저속 냉동 보관법은 4℃ 냉장법이나 압력을 사용하지 않은 다른 냉동 보관법에 비해 우수한 쥐 치아의 치주인대세포의 활성도를 보여 차후 치아의 재식/이식을 위한 중-장기 치아보관을 위한 방법으로의 가능성을 제시하였다. The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at 4℃ for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37℃ water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group. By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.

벡터제어를 이용한 표면형 영구자석 동기전동기의 최대효율제어

이진,김의선,홍형표,최찬학 木浦大學校 工業技術硏究所 1992 工業技術硏究誌 Vol.2 No.-

In general, surface type permenent magent synchronous motor is controlled by the i_d=0 control method for producing maximum torque per ampere below the rated speed. And it is controlled by the field-weakening control method in order to expand the operating speed limits. However, these control methods do not consider the efficiency. So it is required to minimize the loss. This paper proposes a maximum efficiency control algorithm for surface type permanent magnet synchronous motors. The proposed maximum efficiency algorithm is derived from minimizing the core loss and copper loss. In this method the optimum d-axis current is supplied by the vector controller which solves the maximum efficiency control algorithm. From the computer simulation and experimental results. the improvement of the efficiency with the proposed control system was confirmed.

알코올이 Dynamic Posturography에 미치는 영향

이진춘,고의경,장민호,이병주,백무진,노환중,왕수건,전경명 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.2

天安 및 忠淸地域의 産婦人科 應急患者에 관한 臨床的 硏究

李元起,朴忠學,朴鎭完,許義宗,李昌益 단국대학교 1995 論文集 Vol.29 No.-

The objective of this study is to analyze clinical evaluation of 313 patients in Obstetrics and Gynecology who visited Emergency Room in Dankook University Hospital from June, 1994 to December, 1994 for the purpose of successful and perfect emergency care of the patients in Obtetrics and Gynecology in Cheonan-Chungcheong District in the futrure. Today, the goal of modern obstetrics is the well-being of both mother and fetus. And there are many gynecologic diseases which lead the female patient to emergency room with the chief complaint of severe abdominal pain or vaginal bleeding, etc. We have of emergency patients, clinical characteristics of the patients, the time of patient's entrance in emergency room, the monthly distribution of visit, the crowding day of visit, the distribution of the patients according to living area, the admission rate, and the management of the patients, etc. Followings are the results summarized : 1. The mean age was 29.8 years(range form 10 to 85). The mean number of gravidity and parity were 2.0 and 1.1 respectively. The mean number of abortion and living child were 1.0 and 1.0 respectively. 2. The most predominant monthly distribution of visit were August and December. 3. The admission rate was 48.2% and the discharge rate was 49.5%. 4. Distribution of department was Internal Medicine, Pediatrics, Orthopedics, and Obstetrics & Gynecology, in order. 5. The most crowding day of visit was Sunday(22.0%). The peak time of patint's entrance in emergency room was between 20:00 PM and 24:00 PM(24.3%), and the second peak time was between 16:00 PM and 20:00 PM(21.4). 6. Of 313 patients, 53% were known to live in Cheonan city and Cheonan county. 82.7% were known to live in Chungcheong District. 7. In 151 patients who admitted the ward, 53% were obstetric patients and 47% were gynecologic patients. 8. In 80 obstetric patients of admission, the most common chief complaint was labor pain(76.3%). In 71 gynecologic patients of admission, the incidence of disease was abortion(32.3%), ectopic pregnancy(25.4%), and pelvic inflammatory disease(11.3%), in order. 9. Major operations were performed in 40 patients of 151 admitted patients(26.5%). Minor operations including Dilatation and Curettage were performed in 12 patients(7.6%). 10 In 155 patients of discharge, the incidence of disese was abortion(36 patients), pelvic inflammatory disease(20 patients), false labor(15 patients), and uterine bleeding(11 patients), in order.

실리카가 첨가된 PEHA/PS 코어-쉘 압력가소성 고분자의 합성

양의석,김유진,이상석,류상욱 충북대학교 산업과학기술연구소 2011 산업과학기술연구 논문집 Vol.25 No.2

본문참고

유방암에서 E-cadherin의 표출양상에 관한 연구

이상희,김대중,김창진,김의한,양승하 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

Breast carcinoma is common malignant tumor in female of the Korea. Many oncogens have been found in malignant tumor cells. E-cadherin located in cell membrane is important for progression and metastasis of tumor. To evaluate the expression of E-cadherin according to histopathological features and the relation between E-cadherin and prognosis in breast carcinoma, we have performed immunohistochemical staining, using mouse HECD-1 for 65 cases of breast carcinoma. The results were as follows; 1) Breast cacinoma showed loss or marked reduction of the expression of E-cadherin in 41 cases(61.1%) of 65 cases. 2) According to types of breast carcinoma, infiltrating lobular carcinoma, papillary carcinoma, and medullary carcinoma showed higher rates of the loss or marked reduction of the expression of E-cadherin than infiltrating ductal and intraductal carcinoma. 3) According to the location of breast carcinoma, carcinoma developed in right side showed higher rates of the loss or marked reduction of the expression of E-cadherin than left. 4) According to grading of breast carcinoma, cases showing the loss or marked reduction of the expression of E-cadherin was grade Ⅰ 66.7%, grade Ⅱ 55.2%, grade Ⅲ 77.8%. 5) According to absence or presence of lymph node metastasis cases showing the loss or marked reduction of the expression of E-cadherin was 72.4% in non-metastatic cases and 45% in metastatic cases. Above results indicated that the loss or marked reduction of the expression of E-cadherin in breast carcinoma was not related to prognosis of the patients.

피아노후레임 제조기술에 관한 연구

이의길,최양진,조덕호 한밭대학교 산업과학기술연구소 1994 논문집 Vol.2 No.-

Effects of chemical composition, inoculation, shape of graphite and matrix structure upon the mechanical properties and damping capacity of cast iron were investigated. Tensile strength and Rockwell hardness of cast iron were increased by inoculation. The depth of chill and distortion for the manufactures were improved by shape of graphite and matrix structure. The damping capacity was attributable to the characteristic property of graphite.