Characterizations of Modified Silica Nanoparticles(I)

Min, Seong-Kee,Park, Chan-Young,Lee, Won-Ki,Seul, Soo-Duk Materials Research Society of Korea 2012 한국재료학회지 Vol.22 No.6

(3-mercaptopropyl)trimethoxysilane (MPTMS) was used as a silylation agent, and modified silica nanoparticles were prepared by solution polymerization. 2.0 g of silica nanoparticles, 150 ml of toluene, and 20 ml of MPTMS were put into a 300 ml flask, and these mixtures were dispersed with ultrasonic vibration for 60 min. 0.2 g of hydroquinone as an inhibitor and 1 to 2 drops of 2,6-dimethylpyridine as a catalyst were added into the mixture. The mixture was then stirred with a magnetic stirrer for 8 hrs. at room temperature. After the reaction, the mixture was centrifuged for 1 hr. at 6000rpm. After precipitation, 150 ml of ethanol was added, and ultrasonic vibration was applied for 30 min. After the ultrasonic vibration, centrifugation was carried out again for 1 hr. at 6000rpm. Organo-modification of silica nanoparticles with a ${\gamma}$-methacryloxypropyl functional group was successfully achieved by solution polymerization in the ethanol solution. The characteristics of the ${\gamma}$-mercaptopropyl modified silica nanoparticles (MPSN) were examined using X-ray photoelectron spectroscopy (XPS, THERMO VG SCIENTIFIC, MultiLab 2000), a laser scattering system (LSS, TOPCON Co., GLS-1000), Fourier transform infrared spectroscopy (FTIR, JASCO INTERNATIONL CO., FT/IR-4200), scanning electron microscopy (SEM, HITACHI, S-2400), an elemental analysis (EA, Elementar, Vario macro/micro) and a thermogravimetric analysis (TGA, Perkin Elmer, TGA 7, Pyris 1). From the analysis results, the content of the methacryloxypropyl group was 0.98 mmol/g and the conversion rate of acrylamide monomer was 93%. SEM analysis results showed that the organo-modification of ultra-fine particles effectively prevented their agglomeration and improved their dispensability.

초기 자궁내막암 환자에서의 복강경유도하 질식 자궁절제술과 복식 자궁절제술에 대한 비교 연구

김민규 ( Min Kyu Kim ),최동석 ( Dong Seok Choi ),김우영 ( Woo Young Kim ),최철훈 ( Chel Hun Choi ),김태중 ( Tae Joong Kim ),이정원 ( Jeong Won Lee ),김병기 ( Byoung Gie Kim ),이제호 ( Je Ho Lee ),배덕수 ( Duk Soo Bae ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12

목적: 초기 자궁내막암의 수술적치료 시 복강경유도하 질식 자궁절제술 및 골반림프절절제술군과 복식수술군의 수술결과 및 재발율에 대해 비교하고자하였다. 연구 방법: 2003년 3월부터 2005년 5월까지 초기 자궁내막암 환자를 인구학적 특성이 같은 63명을 선택하여 복강경유도하 질식 자궁절제술 및 골반림프절절제술군 26명, 복식수술군 37명으로 나누어 수술 전후 혈색소 차이, 평균수술시간, 취득한 골반림프절갯수, 평균재원일수, 추가치료율, 재발률을 비교하였다. 결과: 복강경유도하 질식 자궁절제술 및 골반림프절절제술을 시행한 군과 복식수술을 시행한 군과의 비교에서 수술 전후 혈색소 차이, 평균수술시간, 취득한 골반림프절 갯수, 평균재원일수, 재발률에 통계적으로 유의한 차이가 없었다. 결론: 초기 자궁내막암의 수술 시 복강경유도하 질식자궁절제술 및 골반림프절절제술 방법이 복식방법에 비해 수술결과 및 재발률에 통계적으로 유의한 차이가 없으며 그 대체 수술법으로 많은 활용이 기대되었다. Objective: To compare laparoscopic surgery with conventional abdominal surgery in patients with early stage endometrial cancer. Methods: A retrospective review of 63 patients with early stage endometrial cancer managed between March 2003 and May 2005. Two groups were defined whether they had been treated by laparoscopy (case group: n=26) or by laparotomy (control group: n=37). We compared age, body mass index (BMI), hemoglobin change, operation time, number of pelvic lymph nodes, hospital stay, case with adjuvant treatment and recurrence between two groups. Results: There was no statistical difference in characteristics (age, BMI, nulliparity, previous abdominal surgery, FIGO stage, histologic grade). between case and control group. In addition, there was no statistical difference in operation data and outcomes between two groups. Hemoglobin changes were 1.1 g/dL (case group) vs 1.7 g/dL (control group) (p=0.072). Operation time was 131 min vs. 115 min. The numbers of lymph nodes obtained were 8.7 vs 7.7 (right) 9.2 vs. 7.6 (left). Hospital stays were 8.4 vs. 9.2 days. Adjuvant treatment cases were 7 vs. 15. Recurrent case was one in each group. Two patients initially evaluated by laparoscopy were converted to laparotomy due to bleeding and adhesion. Conclusion: Laparoscopic surgery for treatment of early endometrial cancer is a safe and effective alternative to laparotomy . However, long-term survival and risk of recurrence have yet to be determined.

남자 알코올 의존 환자의 기질적 특성에 관한 연구

김성곤,민영기,이덕기,김미영,송영상,권도훈,고영훈,박제민,정병선 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.1

목 적: 본 연구의 목적은 한국인 남자 알코올 의존 환자의 기질적 특성을 Temperament and Character Inventory(TCI)를 이용하여 조사하는 것이다. 방 법: DSM-Ⅳ 진단 기준에 맞는 알코올 의존 환자 30명과 정상인 36명을 대상으로 연구학적 특성을 조사하고, 기질적 특성을 한글판 TCI를 이용하여 조사하였다. 모든 대상자의 나이는 30~60세였다. 결 과: 1) 두 군간 평균 나이는 유의한 차이가 없었으나, 교육 정도와 결혼을 유지하고 있는 비율 및 취업률은 알코올 의존군이 정상 대조군에 비해 유의하게 낮았다. 2) 알코올 의존군에서는 정상 대조군에 비해 Harm Avoidance(HA) 점수와 Self-transcendence(ST)점수는 유의하게 높았으며, Self-directiveness(SD)점수는 유의하게 낮았다. 3) 알코올 의존군 내에서의 각 척도들 간의 상관관계는, HA와 SD 점수, HA와 Persistence(PE) 점수, ST와 SD 점수는 각각 유의한 음의 상관관계, ST와 PE 점수, SD 점수와 Cooperativeness(CO) 점수, Reward Dependence(RD)와 CO 점수는 양의 상관관계를 보였다. 결 론: 이러한 본 연구의 결과는 한국인 남성에서 수줍음을 잘 타며, 걱정도 많고, 염세적이고 쉽게 피로해지는 기질적 특성이 알코올 의존과 연관성이 있음을 의미한다. Objectives : The purpose of this study was to assess the temperament of male parients with alcohol dependence. Methods : The subjects were 30 patients who met DSM-Ⅳ criteria for alcohol dependence and 36 normal controls. All subjects completed Temperament and Character Inventory(TCI) which was developed by Cloninger. Results : 1) The educational level, marital status, and occupation in alcohol dependence group were significantly lower than those of normal control group, but there was no difference in the age between two groups. 2) Among the scores of TCI, the score of harm avoidance(HA) and self-transcendence(ST) were significantly higher in the alcohol dependence group than in the normal control group. 3) In the alcohol dependence group, a significant negative correlation among the scores of TCI was between HA and SD, HA and Persistence(PE), ST and SD, and positive correlation between ST and PE, SD and Cooperativeness(CO), Reward Dependence(RD) and CO. Conclusion : These results suggest that shy, fearful, pessimistic and fatigable temperament are related to developing alcohol dependence in Korean males.

젊은 성인에서 흡연에 의한 상완동맥 내피기능의 부전에 관한 연구

이상철,권영주,방덕원,이선해,이보영,김지욱,김영훈,온영근,현민수,김성구 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.1

Background and Objectives : Cigarette smoking is a major factor and the most modifiable risk factor for atherosclerosis. Endothelial dysfunction is an early event in atherosclerosis, before the structural change of the systemic arteries. We hypothesized that endothelial dysfunction might be present in the systemic arteries of young adult smokers and that this might be dose-dependent phenomenon. Therefore, we try to certify that smoking is associated with endothelial dysfunction in healthy young adults without other risk factor of atherosclerosis. Subjects and Methods : we studied noninvasively 29 subjects aged 3 to 4 decades without other risk factor of atherosclerosis, they were composed of the two groups, current smokers(n=15) and nonsmokers(n=14). Smoking history varied from 3 to 20 pack years. Using high-resolution ultrasound, vessel diameter and mean flow rate were measured at rest, during reactive hyperemia(with flow-mediated endothelium-dependent dilation), and after sublingual nitroglycerin(NG-induced endothelium-independent vasodilation). Result : Flow mediated endothelium dependent dilation was observed in the control subjects (9.4±3.44%) but was impaired in the smokers(5.8±2.51%)(p=0.004). NG-induced endothelium independent dilation is no difference in all subjects. Conclusion : Cigarette smoking is associated with endothelial dysfunction in healthy young adults.

항혈청으로 처치된 질트리코모나스(Trichomonas vaginalis) 미세구조의 변화

민득영,류재숙,안명희,박승정,조휘율 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.2

Trichomonas vaginalis is a parasitic flagellate found in the urogenital tract of humans. The present study was performed to evaluate the effect of antiserum from rat and rabbit on fine structure of T. vaginalis. Trophozoites were subcultured in TPS-1 medium(Diamond, 1968) at 37℃, CO₂incubator. T. vaginalis were inoculated in mixed medium containing of antiserum for 10 and 30 minutes, then observed with transmission and scanning electron microscope. The results are as follows: 1. T. vaginalis was ellipsoidal or ovoidal in shape with 4 flagella, an undulating membrane and an axostyle. The plasma membrane of body surface was smooth and furrowed. Pseudopodia(filopods) were often observed by SEM. Ultrastructure of trophozoites consisted of an elliptical shaped nucleus, RERs and Golgi complex around the nucleus, hydrogenosomes, glycogen granules, polysomes and vacuoles in cytoplasm. As the organelles for supporting and movement, an axostyle, kinetosomes, flagella, undulating membrane, costa and parabasal filaments were observed. 2. T. vaginalis treated with antiserum, its surface appeared destruction of plasma membrane, alteration and/or disappearance of axostyle, flagella and undulating membrane under the SEM and showed fine structural changes such as the nucleus surrounded by many vacuoles, decreased ribosomes, which attached to nuclear membrane and RER, vesiculated RER and Golgi complex. The area of filamentous cell coat and a number of coated vesicles were increased on plasma membrane with partial damage. According to going by the incubation time, the progressive destructions of cytoplasmic organelles, nuclear membrane, hydrogenosomal membrane, kinetosome and rootlet fiber were noted. With above results, it is presumed that specific antiserum induced the disturbance of protein systhesis and the damage of cytoplasm resulting lysis of T. vaginalis.

항체 및 보체에 의한 폐흡충(Paragonimus westermani)피낭유충의 형태학적 변화

민득영,김경민,우남식 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.1

The lung fluke, Paragonimus westermani, is widely distributed in Korea and has been well documented as a causative parasite for human paragonimiasis. According to long survival of this parasite against various host defense mechanisms in the infection the disease is persisted, but the fact on host-parasite relationship is still obscure. The present study was investigated to elucidate the effect of specific antibody and complement on metacercaria of lung fluke through the observation of morphological change after incubation of both antibody and complement. Metaceracriae were collected from infected crayfish, Cambaroides similis, and excysted metacercariae were incubated with antibody (3.6mg/100㎕) and complement (fresh rabbit serum) in MEM (pH 7.4) at 36℃, 5% CO₂incubator. The observations with light, scanning (SEM) and transmission (TEM) electron microscope were made 12 and 48 hours after incubation. The results obtained were as follows: 1. Of normal metacercariae three types of papillae (large, small domed and ciliated) and spines were observed on entire body surface with SEM. 2. In metacercariae incubated with antibody and complement, shape of worms was distorted. Flattened surface and partial fusion of paillae were also observed with SEM. 3. Typical syncitium with spines of trematodal tegument was observed with TEM. 4. On the observation of metacercaria incubated with antibody and complement with TEM flattened tegumental surface, degenerated epidermal cells and distorted wide protoplasmic tubules were noticed. With above results it is presumed that antibody and complement might act directly as effectors on functional and morphological impediment of tegument of metacercariae.

전라남도 완도군 보길도 주민의 폐흡충감염 현황조사

민득영 한국건강관리협회 2000 건협 Report Vol.3 No.-

질트리코모나스(Trichomonas vaginalis)의 효소활성에 관한 전자현미경적 관찰

민득영,류재숙,박건채 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

Trichomonas vaginales is one of the parasitic flagellates found in the vaginal cavity of female and the urogenital tract of male. The presnet study was aimde to evaluate the effect of antiserum from immunized Wkstar rats on activities of acid phoshpatase (AcPase) and adenosine triphosphatase (ATPase) of T. vaginalis. Trophozoites were subcultured in Diamond's TPS-1 medium at 37℃ in CO₂ incubator. T. vaginalis were incubated in mixed medium containing 26%(v/v) of antiserum for minutes, then treated with substrates for enzyme activityes. Parasites were embedded in Epon and sectioned for transmission electron microscopy. The results observed are as follows: 1. AcPase activities. In normal prarsites strong activities were observed on Golgi complex and a few of wacuoles. Weak reactions also observed in nuclear matrix, kinetosome and hydrogenosome. In cases treated with antiserum plasma membrane were parially destroyed. Vacuoles were increased in number and revealed strong reactivites. 2. ATPase activities. In normal parasites ATPase were observed dominantly in nucleus. In parasites treated with antiserum activities in nucleus were stronger than those of normal ones, and weak activities were seen along plasma membrane. In conclusion it is assumed that antiserum does increase AcPase activity, but reduces ATPase activity in T. vaginalis.

양측 양성성대질환에 대한 연구

안철민,정덕희,한규철,박상준,이건영 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

Flubendazole의 회충, 편충 및 요충에 대한 구충효과

閔得映,安明姬,金京民 최신의학사 1986 最新醫學 Vol.29 No.10