Irbesartan과 Lercanidipine의 병용요법이 고혈압 및 혈관재형성에 미치는 효과

이도형, 임태완, 조은지, 박현수, 정상혁, 한주희, 병창선 충남대학교 약학대학 의약품개발연구소 2017 藥學論文集 Vol.32 No.-

Hypertension is one of the major risk factor for cardiovascular disease and can lead to or make worse many complications, heart disease, atherosclerosis and diabetes. Thus, managing blood pressure effec tively using therapeutic drugs such as angiotensin II receptor blocker (ARB) and Ca2+ channel blocker (CCB) can be prevent other complications. Therefore, we investigated the synergistic effect of these two drugs, ir besartan and lercanidipine, for antihypertension, cardioprotection and antidiabetes. Irbesartan (28.74 mg/kg, 14.37 mg/kg) or lercanidipine (1.92 mg/kg, 0.96 mg/kg) alone or combination was administered to sponta-neously hypertensive rats (SHRs) and C57BL/6 mice. A two-week treatment of combination with irbesartan and lercanidipine significantly attenuated systolic blood pressure (SBP) and mean arterial pressure (MAP) compared with monotherapy. Futhermore. the combination therapy protected myocardial ischemia reperfusion injury more effectively than monotherapy. In vascular remodeling, combination therapy significantly diminished cuff -induced neointima formation and reduced serum -indllced vascular smooth muscle cells (VSMCs) pro-liferation compared with monotherapy. However, combination therapy had no significant synergistic effect of blood glucose regulation. Taken together, present study suggest that the combination therapy of irbesartan and lercanidipine may be more effective therapeutic strategy for the treatment of hypertension and related complications, myocardial infarction and arterial restenosis than monotherapy

저칼슘식이로 사육한 생쥐의 골수세포 배양에 의한 파골세포양세포의 형성

박주철,강선주,임도선,김현만,고재승 대한구강해부학회 1995 대한구강해부학회지 Vol.19 No.1

Although it is now well established that osteoclasts are derived from mononuclear hematopoietic stem cells, most likely within the granulocyte-macrophage lineage, the exact differentiation process of osteoclast precusors has only been partially discovered. This study was carried out to investigate the following topics by examining the osteoclast-like cell formation from the calcium-deficient mouse bone marrow cells; 1) the effect of calcium deficiency on the formation of the mononuclear phagocytes and the mononuclear precusors of the osteoclast in the mouse bone marrow; 2) the effect of TNF (tumor necrosis factor)-α on the differentiation of osteoclast-like cells from the calcium-deficient mouse bone marrow culture; 3) the formation of the osteoclast-like cell from the calcium-deficient mouse bone marrow mononuclear cells and the relationship between the osteoblast cell line(MC3T3E1) and osteoclast-like cell formation; 4) formation of the osteoclast-like cells from the mononuclear cells which were col1ected from PTH-pretreated mouse bone marrow cells to investigate the relationship between PTH and calcium deficiency in osteoclast-like cell formation. Bone marrow cells were isolated from marrow of mouse feeding normal diet or calcium deficient diet for 7 and 14 days. Bone marrow mononuclear cells were fractionated by cetrifugation on Hypaque-Ficoli density gradients. PTH-pretreated mouse bone marrow mononuclear cells were obtained by collecting the nonadherent cells after the culture of normal mouse marrow cells with PTH for 6 days. The prepared cells were cultured on dentin disc at the concentration of 1.5-2 X 10^(6) cells per 0.5ml of a-MEM containing 10% FCS for 3, 5 and 7 days. Thereafter, TRAP staining, NSE staining, effect of calcitonin on the osteoclast-like cell and scanning electron microscope analysis of resorption lacunae on the dentin disc were performed. The results were as follows; 1. Number of the osteoclast precusors and mononuclear phagocytes in bone marrow was increased by calcium-deficient diet. 2. More osteoclast-like cells were formed from the calcium-deficient mouse bone marrow cells than from normal cells. 3. Formation of osteoclast-like cells was significantly stimulated by TNF-α in normal mouse bone marrow cells and calcium-deficient mouse bone marrow cells at concentration of l0ng/ml and 100ng/ml. 4. Osteoblast cell line(MC3T3E1) did not play an important role in osteoclast-like cell formation. 5. Formation of the osteoclast-like cells from the mononuclear cells which were collected from PTH-pretreated mouse bone marrow cells was similar to that of calcium-deficient mouse bone marrow mononuclear cells, which suggests that the effect of calcium deficiency in osteoclast formation may be mediated by PTH.

국소성 분절성 사구체 경화증의 완전 관해 후 발병한 IgA 신병증 1예

임현민,김현정,박민석,주혜원,최원,김상현,박원도 인제대학교 2006 仁濟醫學 Vol.27 No.-

We report a rare case of primary glomerular disease with IgA nephropathy in a 43-year old man. 7-years ago, he was diagnosed with minimal change nephrotic syndrome. After treatment with steroid for 1-year, azotemia and proteinuria were improved. But, nephrotic range proteinuria was relapsed within 1 week after cessation of steroid. Renal biopsy revealed focal segmental glomerulosclerosis, and achived a complete remission with the use of steroids and cyclophosphamide. Then, he was followed up with normal renal function for 4-years. On admission, the patient showed generalized edema and marked proteinuria. In 3rd renal biopsy, mesangial proliferative glomerulonephritis with diffuse global sclerosis was observed. Electron microscopy showed electron-dense deposit on the mesangium with slight mesangial proliferation, and immunofluoroscence microscopy showed granular deposits of IgA and IgM. These features were consistent with IgA nephropathy, different from results of previous biopsies. The pathogenesis of IgA nephropathy occurred with primary FSGS is not clear, as well as treatment. Now, proteinuria is improving, steroid and cyclophosphamide are probably useful for the disease.

철골커플링 보의 비탄성 이력거동의 해석기법의 제안 및 복합 병렬전단벽구조의 비선형 해석

이주화,박완신,윤현도 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

Ductile coupled flexural walls are the primary seismic load resisting systems of many structures. The coupling beams of these structures must exhibit excellent ductility and energy absorption ability. To achieve better ductility and energy absorption than previously possible, the use of steel coupling beams with their ends embedded in the reinforced concrete walls is proposed. But the present analytical method about behavior of steel coupling beam was scarcely anything. This paper composed of two subjects. First subject is a suggestion of analytical method of steel coupling beam, and selected 5 specimens for that. Second subject is a comparison of Seismic performance with RC coupling beam. This paper used IDARC-2D program for the purpose of achievement these subjects. Suggested analytical method expe3cted that it showed good agreement with steel coupling beam inelastic behavior. Comparison result showed that Steel Coupling beams replacing reinforced concrete coupling beams represent a viable structural system.

인공 석회화 기질의 개발 및 파골세포에 의한 기질흡수에 관한 연구

김현만,박주철,임도선,박수진,김윤지,고재승 대한구강해부학회 1997 대한구강해부학회지 Vol.21 No.1

To develop artificial matrix resorbable by osteoclasts can be used as a model system that biological function of osteoclasts would be assayed in vitro in a standardized condition instead of using heterogenous bone or dentin slices. The present study developed artificial substrates consisted of apatite crystals. collagen gen. agar, apatite crystals under collagen film, mixture of apatite crystals and collagen, and mixture of agar and apatite crystals. And osteoclastic attachement and resorption activity on the each substrate was assayed in vitro. Osteoclasts attached to apatite crystals, collagen, mixture of apatite crystals and collagen, and mixture of apatite crystals and agar, however, not to agar. This suggested that apatite crystals and collagen should be exposed on the surface of artificial substrate where osteoclasts can attach. Osteoclasts attached to the substrate resorbed the substrate consisted of apatite crystals, mixture of apatite crysals and collagen, and apatite crystals under thin film of collagen, however, not of collagen, mixture of apatite crystals and agar. This suggested that the first phase of osteoclasts in resorbing matrix is to secrete acid and the end result of this phase, whatever it may be, may activate secretion of hydrolytic enzymes specific to organic components.

철근콘크리트 커플링 보의 해석기법과 병렬 전단벽의 거동 비교

이주화,박완신,윤현도 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Ductile coupled flexural walls are the primary seismic load resisting systems of many structures. The coupling beams of these structures must exhibit excellent ductility and energy absorption ability. But the present analytical method about nonlinear behavior of reinforced concrete coupling beam scarcely anything. This paper composed of two subjects. First subject is a suggestion of analytical method of diagonally reinforced concrete coupling beam and longitudinally reinforced concrete coupling beam and rhombic coupling beam, and selected 5 specimens for that. Second subject is a comparison of seismic behavior of fully coupled shear wall and partially coupled shear wall. This paper used lDAHC-2D and Midas Gen 4.3.1 for the purpose of achievement these subjects. Suggested analytical method expected that it showed good agreement with reinforced concrete coupling beam inelastic behavior. Also, comparison of seismic behavior result showed that fully coupled shear wall have good behavior than partially coupled shear wall.

제주 지역의 B형 간염 바이러스 유전자형

조지현,박도심,김태현,염주진,김학철,문주현,양재식 대한간학회 2004 Clinical and Molecular Hepatology(대한간학회지) Vol.10 No.1

목적: HBV 감염이 무증상의 자연 회복부터 간경변 혹은 간세포성 간암까지 다양하게 나타나는 요인들로 숙주의 요인과 바이러스의 요인으로 구분되어 제시되는데, 바이러스 요인으로는 감염된 바이러스이 유형이나 염기 변이들이 논의된다. HBV 유형을 구분하는 데는 혈청형과 유전자형이 이용되는데 근자에는 주로 유전자형이 이용된다. 이러한 HBV의 분포는 지역적으로 다르나 지역에 따라서 다른 유형들이 혼재되어 있다. 동남아시아는 B형이지만 극동아시아는 C형으로 알려져 있으며, 대만과 오키나와에서는 B와 C형이 혼재되어 나타난다. 이러한 결과로 미루어 보아 우리 나라의 남부지역에서는 B과 C형이 혼재되어 있을 가능성이 있다. 이에 본 연구는 제주 지역에서 HBV의 유전자형을 조사하고자 하였다. 대상과 방법: 원적지가 제주도이면사, HBsAg가 양성이었던 107명(평균 연령: 46.5세)을 대상으로 하였다. 이들의 혈청으로부터 HBsAg/antiHBe를 분석하고, DNA를 추출하여 7개의 유전자형에 따라 특이성을 갖는 시발체(genotype specific primer)들을 이용하여 PCR로 증폭한 후 이를 분석하였다. 주된 유전자형의 일부와 다른 유형에서 증폭에 이용되었던 시발체를 다르게 이용하여 hemi nested PCR로 증폭한 후 염기를 분석하여 계통발생학적 분석법을 이용하였다. 결과: 1. PCR에 의한 HBV DNA 양성은 107명 중 59명(55.1%)이 양성이었다. HBeAg/anti HBe의 발현 양태에 따라 음성/음성에서 2명(66.7%), 음성/양성에서 30명(41.1%), 양성/음성에서 24명(85.7%), 양성/양성에서는 3명(100%)에서 증폭되었다. 2. Multiplex PCR에서는 58명의 유전자형이 C형, 1명에서는 B형과 C형이 동반 출현하였다. 3. Genotype specific primer를 이용하여 각각 따로 PCR을 시행한 경우에 A형에 의하여 증폭된 예가 1예, B형에 의하여 증폭된 예가 1예, C형에 의하여 증폭된 예가 59예로 A형과 B형 모두가 C형에서 증폭된 예였다. 4. 한 쌍의 시발체에 의하여 증폭되었던 A형과 B형 및 C형의 일부에서 염기를 분석한 결과 모두 C 유전자형이었다. 결론: HBsAg이 양성인 제주 지역 주민의 HBV의 유전자형은 C형일 것으로 생각된다. Background/Aims: Hepatitis B virus (HBV) was classified into 8 genotypes by a sequence divergence in the entire genome designated from A o H. HBV genotypes have distinct geographic distributions. Recently, HBV genotypes have been partially found as influencing the clinical manifestation of chronic liver disease in hosts. In Korea, the distribution of HBV genotypes remains unclear. The aim of this study was to evaluate the prevalence of the HBV genotype on Jeju Island. Methods: Hepatitis B virus genotypes were evaluated among 107 hepatitis B carriers residing on Jeju Island. We used single PCR and multiplex-PCR assay with genotype-specific primer pairs for HBV genotypes A-F for the genotyping. Results: 1. Fifty nine samples (55%) were positive for HBV DNA. The positivity was different according to the pattern of HBeAg/anti-HBe expression, as -/-; 2/3 (66.7%), -/+; 30/73 (30%), +/-; 24/28 (85.7%) and +/+; 3/3 (100%). 2. In the single primer set of genotype-specific PCR, 59 samples (100%) were detected as genotype C and 2 (3%) were also detected as genotype A and B. 3. In multiplex-PCR, 58 samples (98%) were detected as genotype C and only one (2%) as a mixed pattern of genotype B and C. 4. When the PCR products were amplified with universal sense and genotype specific anti-sense from one genotype A, one B, and 2 C, all were included in genotype C. Conclusions: These results suggest that on Juju Island, almost all HBV genotypes are C.(Korean J Hepatol 2004;10:42-50)

항정신병약물을 처음으로 사용하는 초발 정신증 환자에서 나타나는 체중 및 대사성 지표의 변화 : 후향적 연구

김우진,심주철,공보금,강제욱,문정준,김정은,김민걸,박민경,김성진,김현정,정도운 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:This study was to identify weight & metabolic changes in first-episode psychotic patients with antipsychotics use and investigate the differences of weight & metabolic changes between first-episode psychotic patients and controls with antipsychotics use. Method:In this retrospective study, twenty eight first-episode psychotic patients and twenty eight controls with schizophrenia, schizoaffective or bipolar disorder defined by DSM-IV criteria were included. Information about demographic and clinical characteristics of the subjects was gathered from the medical records. Also body weight, sBP, dBP, fasting glucose, lipid profile and WBC count were evaluated before and after antipsychotics use. Rogistic regression was conducted to assess factors associated with weight gain. Results:First-episode psychotic patients showed more weight and BMI changes than controls after antipsychotics use, and these changes continue over 12 months. On the other hand, there were no significant factors associated with weight gain. Conclusion:The results of present study suggest that antispychotics is one of the major causes inducing weight gain of psychotic patients and antipsychotics-induced weight gain is more vulnerable to drug-naive first-episode psychotic patients.

가스 마이크로 검출기의 불감영역(Dead zone)의 연구

김종국,김도현,주관식 명지대학교 자연과학연구소 2004 자연과학논문집 Vol.23 No.-

반도체 공정기법을 도입한 방서선 위치검출기인 가스마이크로 검출기(Gas-Micro Detector)는 높은 효율(both Quantum and Gain)과 우수한 위치분해능(Spatial resolution)을 구현하기 위하여 인가전압을 최대화 하고 양극선과 음극선 간격(half pitch)의 최소화 하려는 연구가 진행되어 왔다. 본 연구에서는 검출기의 수명과 효율에 치명적 손상을 초래하는 전자사태영역(Avajanche Region)에서의 전기장 강화의 한계점과 인가전압에 의한 양극선과 음극선 주위의 등진위선, 전기장선, 그리고 전기장의 세기를 실험과 시뮬레이션(Maxwell program)을 통해 조사하였다. 이 연구로부터 전하수집능력(rate capability)을 저하시키고, 미세한 전극간의 전기장을 교란시킴으로 효율의 불안정과 스파크현상을 초래시켜 검출기의 수명을 손상시키는 불갑영역(Dead Zone)이 존재함을 고찰하였다. Research work for improving image quality has been continued up to the present. Especially, the Gas Micro Detector (GMD) is a new class of radiation detectors in last decade. Due to its good characteristics such as better spatial resolution, better rate capability, higher gain, and lower cost, the GMD may have many potential application as a sensor in medical X-ray imaging, nuclear and high energy physics experiments, and many other fields requiring particle detection and measurement. By continual effort to improve image quality, it need to apply higher voltage and get reduced the spacing between anodes and cathodes in a small cross sectional area. In this work, we present studies of the behavior of gas gain with regard to drift field intensity, anode voltage (Va), drift voltage (Vd), different geometry pitches, electric potential field around cathode-anode strips, time dependence, and several operating parameters for detector's operating. Moreover, estimates of limits of electrical discharge energies are being presented based on computer simulation, Maxwell program package, to explain electrostatic field distortion by charge. From this experimental results, Dead Zone provided a field distortion, a relatively long charge collection time and hence loss of rate capability. We find out which geometric design has to be developed to improve high gain stability as well as detector's lifetime.

양전자 소멸 분광학에 대한 플라스틱 섬광검출기의 시간 분해능 측정

남기용;김도현;김종국;주관식 명지대학교 자연과학연구소 2004 자연과학논문집 Vol.23 No.-

We have measured the time resolution of the system for Positron Annihilation Spectroscopy(PAS). Time resolution means a measure to response to time intervals between start and stop signals. We used ^(22)Na positron source to obtain start and stop signals, that is, 1.27 MeV γ-ray is as a start signal, and 511 keV γ-ray as a stop signals resulted from annihilation with electron in material. The system composed of two plastic scintillation detertors with a size of diameter 2". After calibrating TAC, We obtained that the time resolution of PAS system has about 300 ps for ^(60)Co window. In addition to that, we recognized that the resolution was dependent of the length of delay line on the CFD panel, except of the size of scintillators and the geometry of Photo Multiplier Tube. 본 연구에서는 양전사 소멸 분광학(PSA)의 시스템의 시간 분해능을 측정하였다. 시간 분해능은 시작과 정지신호 사이의 시간 차이의 반응 정도를 측정하는 것이다. ^(22)Na 양전자 동위원소를 사용하여 금속안에서 전자의 소멸로 인한 1.27 MeV의 감마선 시작 신호와, 511 keV의 정지신호를 얻을 수 있었다. 측정장비는 지름이 2인치인 두 개의 플라스틱 섬광검출기로 구성하였다. TAC를 보정한 후에 ^(60)Co 창에서 PAS 시스템의 시간 분해능은 300ps임을 얻을 수 있었다. 이번 연구에서 분해능은 CFD 패널의 시간 지연선의 길이에 의존적임을 알 수가 있었으며, 섬광체의 크기와 광전자증배판(PM Tube)의 구조와는 연관이 없음을 알 수 있다.