마우스 종양발생에서 Nitric Oxide의 역할에 관한 연구 Ⅲ : Helicobacter pylori에 의해 유발된 마우스 위암에서 염증매개인자의 역할 The role of inducible nitric oxide synthase and cyclooxygenase-2 in H. Pylori-associated gastric carcinogenesis

남기택,오상연,조현무,이국경,강진석,제정환,최미나,한상욱,김대용,장동덕,양기화,안병우 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

feficotorfer fyf☞ri(Hp)가 위암파 관련이 있다는 역학적인 증거는 많이 있지만 이에 대한 정확한 기전에 대해선 밝혀져 있지 않고 있으며 실험동물 모델도 적절하지 못한 것으로 알려져있다. 본 실험에서는 위암의 원인으로 알려져 있는 f, fyforf'(Hp)를 이용하여 마우스에서 위암 모델을 확립하고 만성염증과정 중에 생성되는 리0와 COX-2 등의 발현이 위암발생에 미치는 명향을 통하여 예방과 치료를 위한 점근을 시도하고자 하였다. 마우스를 7군으로 나누어서 1, 2, 3, 4군의 등물은 MNU를 증류수에 200ppm 농도로 음수병득 이용하띤 10주간 격주로 투여하였으며 MHU 음술 투여 1주 휴씩 후 배양한 f. fyrofi 를 약 109cru/rfll 로 맞춰 한 마리당 0.1ml 씩 이틀 간격으로 세 번에 걸쳐 하룻방 금식시킨 1, 2, 3, 5, 6, 7군기 마우스의 위장에 투입하떴다. 균 투입을 마친 후 다응 날부터 2군쏙 6군은 iNOS 억제제인 aminoguanidine(AG)을 음수병으로 툰여하였으며 3군과 7군은 COX-2 척제제인 nimesulide(NSD)를 투여하였다. 위의 종양발생양상을 샅최보면 bfNU와 Hp만을 투여한 1 관 ; (hfNU +Hp), 2군 : iNO을 inhibitor 투여군(MNU+HP+AG'1. 3군 ;CO딘-2 Inilibitor 투여군(MNU누Hp누 NSD), 4군 ,MNlf 단독투여군, 5군 ;Hp 단독투여춘, 6군 ; 러p 단독에 AG투여군, 7군 , Hp 단독에 NSD투여군의 종양발샐을은 각각 쁜.Bff(l1/16), 70.6%f12/ti), 했.9ff(7/18), 10%(1/10), Off(O/IS)0%(O/S), 0%(O/5)의 발쟁율을 보여 iNOS 억제제인 AC은 좁양발생을 억계하지 못하였으며, COX-2억제제인 NSD 는 종양발생을 유의적으로 감소시켰다. 콩양발생개수에서는 2.62±0.36, 1.41츠0.14, 0.44 르0.12, 0.10±0.10을 보여 AC와 NSD에서 유의성 있게 발생개수를 줄였다. Hp 단독투여에 의해즌는 종양발생이 나타나지 않았으며 HP+AG, Hp+ IfSD 추여군에콕 시험증료 시점에 약물에 의해 Hp의 제균효과가 있는지의 여부를 확인끓기 위하여 PCR을 이용하여 확인한 결과 모두 양성인 것으로 나타나 Hp의 제균효과에 의한 촐양발생 억제가 일어나지는 않았다. 위의 결과로 볼 때 Hp는 위암발생을 촉진하는 것으로 나타났고 딘p 감염시 매개되는 염증인자를 억제하였을 때 종양발생을 억제하는 것으로 위암 발생에서 염증매개인자는 종양을 촉진하는 것으로 나타났으며 it,705 억제제쓱 COX-』 억제제의 위알 예밭효과fl는 효과적일 것으로 사료된다 In spite of a large volume of epidemiological evidence indicating significant relationship between H. pylori infection and gastric adenocarcinoma, a doubt still exists on an elevated risk of stomach cancer by H. pylori infection due to lack of direct evidence of their exact mechanistic link. It is, therefore, essential to have an appropriate animal model for detailed analysis of the role of H. pylori played in gastric carcinogenesis. There is a wealth of evidence to support that over production of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) is involved in the pathogensis of various cancer in both rodents and humans. The aim of this study was to establish a mouse model for H. pylori-associated gastric carcinogenesis and to identify the role of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) played during the gastric carcinogenesis in mice. Eighty-three specific pathogen free, six-week-old male C57BL/6 mice were randomly divided into seven groups. Animals of the group 1, 2, 3, 4 were given MNU in their drinking water at the concentration of 200 p.p.m. for total five cycles of one-week regimen with one-week pause. After completion of MNU administration, they were given autoclaved distilled water for one weeks, and groups 1, 2, 3, 5, 6, 7 were inoculated with H. pylori. After completion of H. pylori. inoculation, groups 2 and 6 were given aminoguanidine in their drinking water at concentration of 1000p.p.m. and animals of group 3 and 7 were given the diet containing 200 ppm nimesulide at 12 weeks of age. All animals were killed at 50 weeks of age. The incidences of the glandular stomach tumors in the group 1, 2, 3 and 4 were 87.5%(14/16), 76.4%(13/17), 44.4(8/18), 10.0%(1/10), respectively and the tumor incidence of group 3(MNU→Hp+nimesulide) was significantly lower than those of group 1(MNU→Hp) at the value of P<0.01. The average numbers of tumors of group 2(MNU→Hp+AG : 1.41±0.24) and group 3(MNU→Hp+nimesulide : 0.44±0.12) were significantly lower than those of group 1(MNU→Hp : 2.62±0.36) at the value of P<0.05. Therefore, overproduced iNOS and COX-2 plays an important role in mice gastric carcinogenesis. We concluded iNOS and COX-2 inhibitor have good effects on gastric carcinogenesis.

특수효과가 있는 디지털 비디오의 컷 검출

김대환,최연성,고남영 群山大學校 1996 論文集 Vol.23 No.-

Parsing video content is an important first step in the video indexing process. This paper presents algorithms to automate the video parsing task and the presentation key-frame extraction. In this study, we propose a cut detection algorithm using linear coefficients of correlation from edge-map and analyze the existing cut detection methods. Experiments applying to real video clips confirm our methods. Video data modify it for dissolve effect which is fade-out and fade-in. Finally we design a browsing system for video data that use our cut detection algorithm.

생쥐 受精卵의 兩分에 의한 一卵性 雙胎의 生産

金南衡,高大煥 건국대학교 1987 論文集 Vol.24 No.1

These experiments were carried out to produce monozygotic twin by bisection of mouse morula. The morula was bisected by microglass needle without any all of microinstrument or by lateral incision using sharp blade attached to micromanipulator. Bisected deli-embryos were cultured and transferred to pseudopregnant recipient mouse. The results obtained in these experiments were summarized as follows: 1.Of 285 decompacted morulae bisected by microglass needle, 180 embryos were divided into two deli-embryos, respectively, without any visible damage. 2.Total 180 pairs of semi-embryos separated from decompacted morula and subjected to in vitro culture were resulted in 85 (47.2%) pairs of eu-blastocyst, 40 (22.2%) pairs of eu-blastocyst and psuedo-blastocyst, 35 (19.4%) pairs of pseudo-blastocyst and 20 (11.1%) pairs of trophectodermal vesicle and degeneration. 3.Of 255 intact morulae bisected by microblade, 100 (39.2%) embryos were divided into two semi-embryos, respectively, without damage However, 95 (37.3%) embryos were bisected into one normal and one damaged deli-embryos, repectively. The percentage of deli-embryos developed to blastocyst after in vitro culture with and without zona pellucida were 71.4 and 64.9% respectively. 4.Total 15 twins were produced following transfer of the 38 pairs of eu-blastocysts developed from deli-embryos to 25 recipients. However none of pseudo-blastocyst gave birth to young. 5.The percentage of eu-blastocyst cultured with or without zona pellucida and developed to live young following transfer were 11.1 and 9.3% respectively.

탄성지반상의 변단면격자구조물 해석

하대환,정성태,남문희 慶尙大學校生産技術硏究所 1994 生産技術硏究所論文集 Vol.10 No.-

To analyze grid structures on elastic foundation there are several methods, finite difference method, finnite element method, so on. Most of finite element method technique applied structures on an elastic foundation consideres the reaction of the elastic foundation as the vertical reaction at each nodal point, thus that would not includes the reaction of the rotation of the structure on an elastic foundation. Here, the foundation stiffness matrix derived from vertural work theory in introduced to apply structures on an elastic foundation, specialy with linearly varying element.

ERP 수용요인이 회계정보의 유용성과 시스템 만족도에 미치는 영향

이남주,손성진,김성환,강대원 서강대학교 경영연구소 2004 서강경영논총 Vol.15 No.2

Benzene의 감압증류시 응축기 용량과 회수율과의 경제성 연구

김태환,이경주,이대광,원성호,김남기 成均館大學校 科學技術硏究所 1995 論文集 Vol.46 No.1

In order to achieve economic solvent recovery, the recovery ratio was tested on the vacuum distillation of benzene as a solvent by changing the condenser capacity. The results obtained in this experiment were as follows; 1) As increasing the condenser capacity by 6.4 times from 35㎠ to 225㎠, solving recovery ratios were increased by 20%, 24%, and 31% at the bath temperature of 30℃, 40℃, and 50℃ respectively. 2) It was more effective for the solvent recovery ratio to use 10℃ coolant instead of 20℃ coolant, the differences of recovery ratios were about 28%∼32%. The differences of solvent recovery ratios used 0℃ coolant were about 33%∼44% and those used -10℃ coolant were 38.7%∼49%. 3) Bath temperature and solvent recovery ratio are closely related to the condenser capacity. In the case of small condenser capacity, the recovery ratio was increased at low bath temperature. On the other hand, in larger condenser capacity, the recovery ratio was increased at the high bath temperature. From the results of this study we found that the condenser capacity of 55㎡ was the optimal condition for the economic recovery of benzene.

쌍태아에서 제 1태아의 분만 후 제 2태아의 지연분만 : 중례보고

김주환,서민정,유희정,노권일,조대현,박정규,이정헌,조성남,손영수 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

With the widespread use of fertility medications and techniques of in vitro fertilization, multiple gestation has become epidemic in modern obstetric practice. Delivery of the initial fetus in a multiple gestation usually is followed by delivery of the subsequent fetus or fetuses shortly thereafter. However in rare circumstances, the delivery is delayed for days due to disappearance of uterine contraction after delivery of the first fetus. When uterine activity ceases after the delivery of the first neonate, a policy of nonintervention may be considered in case of an immature or very premature delivery, in order to achieve a gestational age for the remaining fetus(es) more compatible with neonatal survival. We report a case of twin gestation with prolongation of the delivery interval between the twins for 46 days.

러시아 농업과학기술 연구기관과 운영체체의 변화

이상덕,김종환,정남진,오대근,오성종 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.2

This work is dedicated to main directions of Agricultural science policy of Russia during the transition to the market economy. In this study, Russian R&D system, R&D expenditure and structure of research institutes were introduced. Especially, Studies on organizations such as RFBR : Russian Foundation for Basic Research, RFTD : Russian Fund for Technological Development, Russian Foundation for Promotion of Small Enterprises in S&T, FPP : Fund for presidential Programs, SRC : State Research Center, ISTC : International Science & Technology Center were carried out. Analysis of the problems of Agricultural Science sector of Russia shows the situation is very complicated. Despite all efforts of Government, the national institutes is still in crisis. It is clear today that without energetic and intelligent reforms, it would be impossible to implement resonable reorganization of Agricultural sector and have to strong and deserved science and technical sector in Russia.

유전자 재조합 사람 IFN-beta인 제네베타와 베네세린의 생물학적 활성의 비교

하종천,이협준,박봉환,김진숙,유대근,남상윤 전주대학교 자연과학종합연구소 2003 전주대학교 자연과학연구소 학술논문집 Vol.15 No.1

Background: Recombinant IFN-beta products have been approved for the treatment of several diseases. Recently, Inbionet, Co. has successfully developed rhIFN-beta product, Genebeta. This study was performed in a purpose of confirming the biological activity of Genebeta by comparison with another recombinant IFN-beta, Beneserin which was already approved. Methods: In vitro antiporliferative activity, regulation of MHC class I expression, antiviral activity, regulation of natural killer cell cytotoxicity were studied for functional comparison of these two rhIFN-beta products. Results: Proliferation assay data showed that two recombinant IFN-beta inhibited T cell and Daudi cell proliferation to a similar extent. When antiviral activity was compared, there was no significant difference between them. The treatment of these two rhIFN-beta also resulted in a similar level of upregulation of class I expression on the surface of A549 cells. Cytotoxicity experiment against tumor cells revealed that both of rhIFN-beta could enhance NK cell cytotoxicity to comparable level. Conclusion: All comparison data indicated that rhIFN-beta products, Genebeta and Beneserin have comparable potentials as a biological response modifier.

Benzoate, m-Toluate, Phenol의 Pseudomonas putida에 의한 생분해

김장규,김석형,김태환,이대광,김남기 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.1

It this study, basic data were obtained for the treatment of industrial waste water which includes aromatic compounds. Substrate concentrations used were 100ppm, 500ppm, and 1,000pprn and initial pH's were pH6, pH7, and pH8 in order to obtain the optimum conditions for treating benzoate, m-toluate, and phenol by Pseudomonas putida(KCTC 1644). Durations were 20hrs for 100ppm, 40hrs for 500ppm, and 60hrs for 1,000ppm before the static growth of Pseudomonas putida. 100% of 100ppm benzoate(0.31g cell/1) was biodegraded at pH6, pH7, and pH8 before 20hrs, 52.8% of 500ppm at pH8 in 40hrs, and 27.9% of 1,000ppm at pH8 in 60hrs. The best initial pH was pH8 for the biodegradation and the growth of Pseudomonas putida in benzoate medium. For m-toluate, the best initial pH was also pH8. At this pH, 40.6% of 10Dppm(0.31 cell/1), 21.9% of 500ppm, and 14.1% of 1,000ppm m-toluate were biodegraded respectively in limited time. But for phenol, the best initial pH was pH7. At this point, 9.8% of 100ppm(0.218 cell/1), 7.1% of 500ppm, and 4.7% of 1,000ppm phenol were biodegraded respectively. Therefore, the best carbon source in this experiment was benzoate. Pseudomonas putida was also able to biodegrade m-cresol, p-hydroxybenzoic acid, and benzaldehyde.