Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

T 및 B cell 測定 : 1. 1次 成績 Ⅰ. Results, Primary

金在植,崔成萬,金在龍,全東錫,朴正姬,徐相喆,金仁子,金在崇,金重明 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.2

E-rosette法에 의한 T cell의 百分率과 螢光抗體法, 感作牛赤血球擔體法, 酵素抗體法 및 마우스赤血球 rosette法에 의한 B cell 의 百分率을 檢査하여 다음과 같이 요약할 수 있었다. 정상성인군의 active T cell은 26±8.2%이었고 total T cell은 72±4.9%이었다. B cell 는 螢光抗體法으로 22±5.4%, 感作牛赤血球擔體法으로 15.8±4.9%, 酵素抗體法으로 15±5.2% 그리고 mouse RBC-rosette法으로 17.2±4.1%이었다. 惡性腫瘍에 있어서 active T cell은 27.±10.4%이었고 total T cell 은 59±11.2%로서 정상인군에 비하여 유의한 감소를 보였다. 良性疾患에 있어서 T cell 은 정상인군에 비하여 유의한 차이를 볼 수 없었다. T cell percent by E-rosette and B cell by fluorescent antibody, senitized ox RBC rosette, enzyme antibody and mouse RBC rosette tests were performed. In healthy adult group the active T cell was 26±8.2% and total T cell was 72±4.9%, B cell by fluorescent antibody was 22.±5.40%, by ox RBC rosette 15.8±4.9%, by enzyme antibody 15±5.2% and by RBC-rosette 17.2±4.1%. In malignant tumor the active T cell was 27±10.4% and total T cell was 59±11.2%. In benign diseases T cell count showed no significant difference from normal adult group.

패러글라이딩 사고에 대한 분석

김승환,김인병,정순미,정성필,장석준 대한외상학회 1999 大韓外傷學會誌 Vol.12 No.1

$quot;Background: Paragliding injuries are increasing recently due to the growing popularity of this sport. This topic has been discussed in numerous journals in the European countries with large mountains, such as Austria, Germany and Switzerland, Those studies gave information regarding the flight phase during which an injury ocurred, the type and site of injury, and suspected reasons for the accidents. This study was done to compare our data to those of other nations and give preventive advices to reduce injuries occurring during paragliding. Method: Nineteen paragliders documented as having injuries during paragliding at Chung-Ju, Korea from 1990 to 1997 were included in the study. Their accident reports including medical records, were analyzed retrospectively. Result: Eighteen of the injured were male with one female and their mean age was 25.6 years old. Five injuries were suffered during take-off, of which technical error was the cause in most cases. Also, 5 injuries occurred during flight due to a sudden change of thermal and wind condi- tions. The majority of injuries were suffered during the landing period, affecting 9 paragliders. They were mostly due to forced landings, unexpected obstacles, and sudden change of wind conditions. Thirty-three percent suffered spine trauma and 29% were involved in injuries of the lower extremities. Upper limb injuries occurred in 19%, and 19% also suffered cerebral concussion or contusion. Conclusion: We have found that spine and ankle injuries were commonly occur during paragliding, especially the landing phase. Proper knowledge of the procedures, adequate training and appropriate foot wear could help in reducing injuries while paragliding.$quot;

생체분해성 망막압정을 이용한 망막고정에 대한 실험적 연구

김용백,민병무,김창식,박근성,김승영,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Biodegradable retinal fixation devices obtain mechnical fixation of the retina with desirable chorioretinal scarring and with the potential for local, sustained release of antimetabolites and steroids to inhibit proliferative vitreoretinopathy. We manufactured a biodegradable retinal tack with barb that was designed in order to prevent intrusion from implantation of retinal tacks. This study was carried to evaluate the efficacy for retinal fixation and the capability for sustained release of drugs with a newly designed biodegradable retinal tack Biodegradable retinal tacks were made of polymers of glycolic acids and were designed with barbs in a shape to prevent the disinsertion. Biodegradale retinal tacks are divided into 3 parts, a conical portion that is inserted into the sclera, a cylinder portion that remains in the vitreous, and a neck portion between the pin and the cylinder. The tapered conical end was manufactured to allow easy insertion through the retina and choroid into the sclera. A cylinder portion was manufactured with a tapered angle that fixes firmly into the orifice of 19 gauge spinal needle. A neck portion, 0.4 mm in diameter, was designed to prevent disinsertion from following implantation of retinal tack. The applicator was a 19 gauge spinal needle and its orifice was prepared to 15°angle to accept the tapered cylinder portion of the retinal tack. The retinal tacks, secured in the needles, were passed through the formed vitreous and inserted into the retina, choroid, and sclera and were released by pushing the internal needle, usually within 2-3mm of the medullary ray of the posterior rabbit retina A retinal tack was placed in each of 8 pigmented rabbit eyes. Slit-lamp biomicroscopy, indirect ophthalmoscopy and fundus photography were performed periodically from 1 day to 8 weeks after surgery. Eight eyes were enucleated and studied by light microscopy at 8 weeks. Biomicroscopic evaluation of the animals revealed edemas adjacent to the retinal surfaces immediately after insertion of the biodegradable retinal tacks in all the animals. These edemas disappeared after 1 week. The first noticeable change in the size of retinal tacks was shown after 2weeks. The size of the retinal tacks gradually got smaller, decreasing to about one-half at 4 weeks and about one-third at 8 weeks. All retinal tacks remained in inserted places without any movement for an 8 week period. On light microscopy, epiretinal proliferations were seen to extend into the vitreous cavity. Cellular capsules that lined the inner aspect of the scleral defect caused by tack insertion were found. However the adjacent retina had a normal cytologic appearance and architecture in all specimens. We manufactured a biodegradable retinal tack that is designed to prevent intrusion from implantation of retinal tacks. All biodegradable retinal tacks reduce in size with time, but no retinal tacks extruded from the inserted place. The newly designed biodegradable retinal tack can be used for retinal fixation and may be used as a vehicle for the introduction of pharmacologic agents to prevent the cellular events that promote proliferative vitreoretinopathy.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

이온주입이 금속의 일함수에 미치는 영향(Ⅰ)

김현경,강석태,황정남,이철주,최대선,정병두 연세대학교 자연과학연구소 1984 學術論文集 Vol.13 No.-

이온 주입이 일함수 차이에 미치는 영향을 연구하기 위하여 낮은 에너지 이온 가속기, 전자총과 기타 일함수 차이를 측정하기 위한 장치를 설계, 제작하였다. 제작된 이온 가속기의 집속 특성은 이론적인 값과 일치하였다. 즉, 집속 렌즈의 확대율이 이론값은 2.0인데 비하여 1.2로 상당히 작은 값이었다. 또한 집속 렌즈의 전압 V_L과 추출 전압 V_e에 의한 집속 특성 역시 이론값과 매우 잘 일치하였다 . 제작된 장치를 사용하여 다결정 Cu와 Ni의 일함수 차이를 측정한 결과 0.45eV임을 알았다. 그리고 전자선을 이용하여 억제 전위차로 일함수를 측정할때 입사 전자의 반사율을 고려해야 함을 알았다. Low energy ion accelerator, electron gun, and apparatus for measuring work function difference are designed and constructed in order to study the effect of ion implantation on the work function. The focusing properties of accelerator is coincided with theoretical results; the magnification of Einzel lens is found to be 1.2, while theoretical value is 2.0, and the focal properties as a function of V_L/V_e are in good agreement with theory. Using these systems, the work function difference between polycrystalline Cu and Ni is found to be 0.45eV, and the experimental retarding potential plots indicate close agreement with the theoretical values modified by electron reflection.

한우 치은의 Collagen 함량에 관한 연구

김석환,정태영 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

This investigation was performed to estimate the proteins, chiefly collagen, obtained from 2 year old bovine gingival tissues. Proteins in samples were extracted by salt or pepsin digestion. Extracted collagens were treated with 0.1% SDS and 0.1% β-mercaptoethanol in 0.01M phosphate buffer, pH 7.0, and fractionated by SDS-agarose gel column chromatography and/or SDS-polyacrylamide gel electrophoresis. The hydroxyproline content of salt-soluble and pepsin-soluble collagen was also determined. The results were as follows : 1. The content of hydroxyproline in gingival tissues was 54㎍/mg dry weight. The content of hydroxyproline in salt-soluble collagen was high than that in pepsin-soluble. 2. From both of salt-soluble and pepsin-soluble collagen, the bands of α_1-, α_2-chain, and the unknown band corresponding to molecular weight 68,000 were separated by SDS-polyacrylamide gel electrophoresis. The another band of β-chain was also separated from the salt-soluble collagen. 3. The recollected fractions under the peaks of SDS-agarose gel column chromatography were separated by SDS-polyacrylamide gel electrophoresis into the bands of α_1-and α_2-chain around the area corresponding to the molecular weight of collagen.

역행성 골수강내 금속정을 이용한 대퇴골 과상-과간 골절의 치료

정필현,황정수,강 석,김종필,전용진 대한골절학회 2004 대한골절학회지 Vol.17 No.2

사이토카인과 Lipopolysaccharide 자극에 의한 RAW 264.7 세포주의 nitric oxide 생성

김영덕,전창덕,이병순,이복수,박석돈,백상기,정헌택 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

Macrophages have been implicated as a major class of effector in the host response to neoplasia. Cells of the monocyte-macrophage lineage are known to exhibit tumoricidal activity following stim-ulation by γ-interferon, tumor necrosis factor, BCG and bacterial products such as lipopoly-saccharide (LPS). While the mechanism involved remain obscure, the generation of reactive nitrogen intermediate (RNI) by activated macrophages is considered a maior participant in mediating the tumorstatic effect. But much of what is known about the induction and release of RNI has been elucidated by using freshly isolated cells from blood and other tissues of experimental animals. In this study, we used a murine macrophage cell line, RAW 264.7, and found that these cells showed above 99% positive of pan macrophage marker by immunohistochemical staining. These cells could produce nitric oxide (NO), when incubated with γ-IFN or poly I:C. Incubation of RAW 264.7 cells with γ-IFN for 48 hous in the presence of LPS agumented NO release in a dose dependent manner. Whereas, treatment of anti-TNE-α antibody or antisense TNF-α oligodeoxynucleotide inhibited the release of NO_2 by γ-IFN plus LPS activated macrophages. The production of NO was also inhibited reversibly or irreversibly by N^GMMA,NAA,arginase or DPI. Thease data suggest that RAW 264.7 cell line may be useful for the in vitro evalulation of biological response modifiers as well as the study of signal pathway of NO release by macrophages.

Al₂O₃섬유강화-알루미늄 금속 복합 재료에 첨가된 Li의 영향

金錫胤,金政根 弘益大學校 1989 弘大論叢 Vol.21 No.2

Samples of FP(Al₂O₃) fiber/Al-2.5Li metal matrix composite have been fabricated by Liquid metal pressure forming technique. The fiber orientation in the composite was unidirectional to the specimen axis. The ?? of the specimen was 468 MPa and especially young's modulus was very high enough to 150.9GPa owing to the good bonding between fiber and matrix. The fracture surfaces exhibited that the cracks were propagated in a mixed mode, that is, fibers bread with the matrix, debonding and pullout from the matrix until a final fracture took place. The interfacial delaminated dendritic structure were also observed. Li addition increased the wettability of the fiber in the matrix, because of the formation of two compounds in the interfaces, namely, α-LiAlO₂and LiAl??O??.