Polyphosphate가 Porphyromonas endodontalis의 성장에 미치는 영향에 관한 연구

최성백,최호영,민병순,박상진,이진용,최기운 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.1

Polyphosphate has been used to prevent decomposition of foods and has been shown to have inhibitory effect on the growth of gram positive bacteria. The purpose of this study was to evaluate the effect of polyphosphate on the growth of Porphyromonas endodontalis, a gram negative endodontopathic bacterium. Porphyromonas endodontalis ATCC 35406 was grown in the presence of polyphosphates with different chain lengths. Inhibitory effect of each polyphosphate which was added at the beginnig or during the culture, was determined by measuring the optical density of the bacterial cell at 540nm and by viable cell count. The results from this study were as follows : 1. Polyphosphates were shown the growth inhibition of the Porphyromonas endodontalis. 2. The minimal inhibitory concentration(MIC) of polyphosphate was observed to be 0.04%. 3. Polyphosphates with chain lengths of 25 and 75 demonstrated the greatest inhibitory effect on the growth of Porphyromonas endodontalis. 4. Polyphosphates are bactericidal to Porphyromonas endodontalis, demonstrating the growth inhibition of the bacterium. The overall results suggest that use of polyphosphate may affect the growth of Porphyromonas endodontalis. Further studies will be needed to confirm the effect of Polyphosphate.

扶正抗癌湯이 抗癌作用 및 免疫機能에 미치는 影響

최운영,김강산,강병기 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1

Bujeonghangam-tang(BHT) has been used in Oriental Medical Hospital of WonKwang University as a drug anti-cancer in recently. So, the purpose of this study was to investigate effect of BHT on the proliferation of anti-tumor, immunocytes and nitric oxide(NO) production from mice peritoneal macrophages. BHT consists of the following components ; Coicis semen(薏苡仁), Lilii bulbus(百合), Testudinis plastrum(龜板) Acanthopanacis cortex(五加皮), Ginseng radix(人蔘), Atractylodis macrocephalae rhizoma(白朮), Corni fructus(山茱萸), Asparagi radix(天門冬), Proraleae fructus(補骨脂). This study estimated the proliferation of L1210 cell lines, mouse thymocytes and mouse splenocytes and nitric oxide(NO) production from peritoneal macrophages in vitro. and estimated the proliferation of L1210 cells, thymocytes and splenocytes, NO production from peritoneal macrophages in L1210 cells-transplanted mice in vivo. The proliferation and cytotoxicity of cells was tested using a colorimetric tetrazoliun assay(MTT assay). The results of this study were obtained as follow ; BHT inhibited on the proliferation of L1210 cell lines in vitro and in vivo, and BHT was accelate nitric oxide prodution from peritoneal macrophages in vivo. This results sugest that BHT have anti-cancer action by NO production from peritoneal macrophages.

TV 요리프로그램 진행자의 전문성 여부에 따른 구성내용의 질에 대한 시청자의 평가

김운주,최은희,김기현,김정숙,송현숙,정은옥 충북대학교 교육 ·생활연구소 생활과학연구센터 2002 생활과학연구논총 Vol.6 No.1

The purpose of this study was to develop the instrument to assess the TV cooking program contents and to indentify the difference of cooking program contents according to professionalism of MC. The evaluation sheet was composed of 5 sectors(sanitary practices, cooking process, information & proceeding, table setting & tasting, applicability) with all 32 questions, 5-Likert scale was used. College students majoring in food & nutrition were very interested in cooking program and 72.4% of respondents knew the cable TV Food channel. The major source of cooking information was TV(51.5%). The comparison of four TV cooking programs with professional MC(2 program) and amateur MC(2 program) was performed. Except applicability sector, the mean evaluation scores of professional cooking program were higher than amateur cooking program. In the amateur MC program, 'hand washing', 'use of hair restraints', 'separate use of cutting board', 'dish towel cleanliness', 'separation of service table', 'taking a chair in tasting', 'use of individual dish' items rated low rating score(<2.00) so more control was needed. The findings of this research suggest that cooking professional should be participate in developing TV cooking program as MC or staff.

돈분과 피마자박의 혼합비율에 따른 퇴비화 연구

장기운,이종진,홍주화,김남천,김완주,최우영 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This study was conducted to estimate the humificating grade according to experiment of physico-chemical characterization and phyto-toxicity during composting of using with pig manure and castor meal. The material ratios of the compost which was mixed with pig manure(P), castor meal(C), and saw dust(S) were 5 : 1 : 4(PCS-I), 5 : 2.5 : 2.5(PCS-II) and 5 : 4 : 1(PCS-III) by volume to volume, and they were decomposed for 60 days. In the result, the changes of temperature in all treatments during composting were rapidly increased more than 68℃ at the incipient stage, and gradually decreased within 39℃ at 60 days the after treatment. pH was slowly increased from 7.5 to 7.7, and the C/N ratio was 13∼14 at the final composting stage. The low C/N ratio value in this compost was caused by the castor meal contented high nitrogen level(T-N 5.7 %). G.I.(germination index) was showed 73 to 78 range in all treatments at the 60^(th) day. Among all treatments PCS-I was appeared to be the best condition for composting.

쌀보리를 기질로 한 알코올발효의 최적 액화효소

남기두,김운식,최명호,박완 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

증자시 높은 점성이 문제되는 쌀보리를 기질로 알콜발효를 행할 경우, 기질의 점성문제를 해결하여 발효조건을 개선하면서 생산수율을 올릴 수 있는 상업용 액화효소를 검토하였다. 쌀보리 증자액화시 BAN을 사용한 경우 기절의 점도는 T120L의 1/3 정도로 떨어졌으며 최종 알콜수율도 T120L에 비해 원료 Ton 당 4 ℓ 정도 더 높게 나왔다. 알콜의 수율은 사용한 당화효소의 종류에 의해서도 영향을 받았다. 산업 규모에서 BAN을 사용한 쌀보리의저온증자가 가능하였으며 고온증자에 비해 알콜수율도 388.4에서 390.4 ℓ/Ton으로 약간 증가하였다. 또 이때 사용한 기질의 전분가를 분석하였는데, 산가수분해 및 효소가수분해법에 의한 쌀의 전분가 분석치는 큰 차이를 보이지 않았으나 쌀 보리의 경우는 효소가수분해법에 의한 값이 산가수분해법에 비해 10% 정도 낮게 나타났다. 이때 BAN은 T120L에 비해 전분가가 약 1% 높게 나왔다. Various treatments of naked barley with commercial liquefying enzymes have been employed to reduce high viscosity of naked barley in cooking as a raw material for alcohol production and to increase alcohol yield. The enzyme BAN used for cooking and liquefaction of naked barley was able to make a reduction of one third of viscosity and to enhance alcohol yield of 4 l/Ton of raw material than the T120L was. Of course, alcohol yield depended in part on the applied saccharifying enzymes. The low temperature cooking of naked barley with BAN was favorable compared with high temperature cooking of both of reducing viscosity (210 vs. 237 cp) and final alcohol yield (Yp/so; 0.397 vs. 0.395 g/g) in industrial scale.

금강 상류수계 봉황천 및 주자천의 어류상

문운기,박경서,최신석,홍영표 忠南大學校 環境問題硏究所 2001 環境硏究 Vol.19 No.-

용담댐의 호소화에 의한 어류군집 변화를 알아보기 위하여 2001년 5월부터 8월까지 봉황천과 주자천을 중심으로 어류상 및 어류군집 양상을 조사하였다. 조사 결과 본 지역의 하천형은 Aa, Aa-Bb형이었다. 본 조사에서 6과 17속 19종이 출현하였다. 봉황천에서는 12종 464개체, 주자천에서는 14종 682개체가 채집되었다. 각 하천에서 우점종은 Zacco temmincki, 아우점종은 Zacco platypus이었다. 한국 고유종은 전체 19종 중 10종이었고, 전체 어종수의 52.63%로 매우 높은 비율을 보였다. 종의 다양도는 봉황천에서는 지점 4가, 주자천을 포함한 본류수계에서는 지점 9와 10이 높게 나도, 균등도와 우점도는 각각 0.616, 5.884, 0.482, 0.410이었다. 특히, 댐의 건설에 따라 댐 형성지점에서 Z. platypus와 Opsarlichthys bidens의 집단증가가 주목되었다. Formaing a part of fish community, the fish fauna species composition, and species diversity were investigated in the Bonghwang-Cheon and Juja-Cheon Stream, the upper Kum-Gang River of Korea from May to August in 2001. In the most of sampling area, the type of streams were Aa and Aa - Bb. As a whole, the collected fishes were classified 19 species and 17 genera belonging to six families. Twelve species, 464 individuals in the Bonghwang-Cheon Stream, and 14 species 682 individuals in the Juja-Cheon Stream were collected. In each stream, dominant and subdominant species was Zacco temmincki and Zacco platypus. Ten korean endemic species (52.63) were collected during the sampling period. Diversity(H'), richness(RI), evenness(EI), and dominance index(λ) of the fish communities were 0.616, 5.884, 0.482 and 0.410, respectively. By the means of physical enviroment change by Yongdam Dam construction, increasing of Z. platypus and Opsarichthys bidens population was remarked.

제2형 당뇨병 남성 환자에서 유리 테스토스테론과 성호르몬 결합 글로블린 농도

남기덕,김영설,박철영,오승준,김덕윤,우정택,김성운,양인명,김진우,최영길 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.6

연구배경:인슐린저항성은 제2형 당뇨병과 심혈관질환의 주요한 위험 인자로 성호르몬과 상호 관계가 있다고 알려져 있다. 그러나 여성과는 달리 남성에서는 연령과 인슐린저항성에 따른 유리 테스토스테론과 성호르몬 결합 글로블린 농도의 변화에 대한 연구가 부족한 실정이다. 그러므로, 본 저자등은 제2형 당뇨병 남성 환자에서 정산인과 비교해서 유리 테스토스테론과 성호르몬 결합 글로블린 농도를 측정하고 연령에 따른 변화 정도를 알아보고자 하였다. 방법:대상 환자 모두에서 연령과 체질량지수, 총 콜레스테롤, 중성지방, 공복혈당과 인슐린 농도를 측정하였다. 혈중 유리 테스토스테론 농도는 방사면역측정법(radioimmunoassay)을 이용해서 측정하였고, 혈중 성호르몬 결합글로불린은 면역방사계측측정법(immunoradiometric assay)을 이용해서 측정하였다(Diagnostic System Laoratories, Wbster, TX, USA). 결과:1)제2형 당뇨병 남성 환자에서 정상 대조군 남성에 비해 성호르몬 결합글로불린은 104.1±35.0 vs 25.7±3.5 mole×10??로 의미 있게 높았으나(p<0.001), 유리 테스토스테론은 13.7±9.5 vs 13.6±6.5 ng/dL로 차이가 없었다. 2)연령과 성호르몬 결합 글로블린 사이의 상관 계수는 제2형 당뇨병 남성 환자에서 0.40로 중등고의 양의 상관 관계를 보였고(p<0.001), 정상 대조군 남성에서 0.11로 유의한 상관 관계를 보이지 않았다. 연령과 유리 테스토스테론 사이의 상관 계수는 제2형 당뇨병 남성 환자에서 0.08, 정상 대조군 남성에서-0.17로 모두에서 유의한 상관 관계를 보이지 않았다. 3)연령과 체질량지수를 보정한 후에 제2형 당뇨병 남성 환자와 정상 대조군 남성에서 혈중 인슐린 농도, 유리 테스토스테론과 성호르몬 결합 글로블린 사이에는 상관 관계가 없었다. 결론:제2형 당뇨병 남성 환자에서 정상 남성에 비해 성호르몬 결합 글로블린 농도가 증가되어 있었으며, 유리 테스토스테론은 차이가 없었다. 연령이 증가함에 따라 성호르몬 결합 글로블린이 제2형 당뇨병 남성 환자에서 정상 남성에 비해 증가 폭이 의미있게 컸으며, 유리 테스토스테론은 변화가 없었다. Background: Insulin resistance is a risk factor for cardiovascular disease and type 2 diabetes mellitus. There are many previous studies indicating that insulin lowers serum sex hormone-binding globulin levels, and there is inverse correlation between insulin resistance and serum sex hormone-binding globulin levels in women. However, in men, a limited number of studies are available to explain the effect of sex hormone on age and insulin. Therefore, the present study was undertaken to investigate the relationship among free testosterone, hormone-binding globulin and age in type 2 diabetic men and control subjects. Method: Age, body mass index, total cholesterol, triglyceride, fasting blood sugar, and insulin concentrations were examined on 89 type 2 diabetic men and 47 control subjects. The free testosterone level was measured by commercially available double-antibody system (Radioimmunoassay). The sex hormone-binding globulin level was also measured by commercially available double-antibody system(Immunoradiometric assay). Results: 1) Sex hormone-binding globulin level was significantly increased in patients with type 2 diabetes. However, there was no significantly difference in free testosterone level between the two groups. 2) Sex hormone-binding globulin was positively correlated with age (r=0.4, p <0.001) in patients with type 2 diabetes. Sex hormone-binding globulin and free testosterone were not correlated with age in control sujects. 3) Free testosterone and sex hormone-binding globulin concentrations were not significantly related to serum insulin concentration after adjusting for age and body mass index. Conclusions: We observed increased sex hormone-binding globulin concentration in diabetes man, and was a positively related to age. Further studies are needed to understand the relationships between age, insulin resistance, testosterone, and sex hormone-binding globulin concentrations(J Kor Diabetes Asso 24:699~707,2000).

Streptococcus salivarius의 요소분해효소 활성에 관한 연구

정상백,최호영,민병순,박상진,이진용,최기운 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.1

Dental caries is induced by organic acids produced by oral bacteria. In order to prevent dental caries, therefore, it is essential to maintain neutral pH in the oral cavity. Urea plays a major role in oral pH homeostasis. Urea is hydrolyzed by bacterial ureases to ammonia, causing a pH elevation, Streptococcus salivarius has been shown to be a major contribution to oral ureolysis. Synthesis of urease by S. salivarius appears to be constituitive, but can be greatly enhanced by low pH. It is, therefore, conceivable that ureolytic activity of S. salivarius from a carious lesion is greater than that of the bacterium from a healthy tooth. In the present study, urease activity of S. salivarius isolates from dental plaque of carious lesions was compared with that of the isolates from plaques of the teeth and the dorsum of the tongue; 45 S. salivarius strains were isolated from carious lesions(>C2) of 21 individuals with dental caries and 30 strains from 10 individuals without dental caries. The results were as follows: 1. All the 21 individuals with dental caries harbored ureolytic S. salivarius whereas 3 of 13 individuals without dental caries harbored non-ureolytic strains of S. salivarius. 2. All the 45 S. salivarius isolates from carious lesions showed urease activity. In contrast, of 30 isolates from individuals without dental caries, 17 isolates(56.7%) did not demonstrate urease activity, or if any, very little(<5μmol/min/mg). 3. Urease activity of the isolates from carious lesions was greater than that of the isolates from individuals without dental caries:the urease activity ranged from 42 to 381μmol/min/mg and from 0 to 208μmol/min/mg, respectively. 4. At acid pH(5.5), the isolates which showed intermediate urease activity at pH 7.0 demonstrated even higher activity whereas the isolate with no or lower urease activity did not show any significant difference in their activity. However, the isolates with the greatest urease activity from both individuals with and without dental caries, exhibited a rather much lower urease activity at pH 5.5. The overall results suggest that isolates may have their own urease activity but the isolates exposed to chronic acidic environment of the carious lesion might elevate urease activity of S. salivarius, which in turn, might influence on survival of S. salivarius itself and other bacteria establishing a new oral bacterial ecosystem.

표면처리된 복합레진에 대한 수리용 레진의 결합강도에 관한 연구

강현숙,최호영,민병순,박상진,최기운 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

Composite resin repair requires strong bond strength between the new and old materials. The objective of the current study was to identify the optimal treatments for sufficient bond strengths. Bondings between same kinds of materials and cross bondings using chemical curing composites and light curing composites were tested. Surface treatments included the methods of sand-blasting, acid etching and coating of bonding agent. Seven kinds cases of combinations from three kinds of methods were experimented and compared with a control group of which surfaces were highly polished. Measurements of shear bond strength and observations of surface morphologic changes using a scanning electron microscope were done. Following conclusions were drawn 1. The highest bond strength among composite resins were exhibited by the treatment of the sand-blasting and the coating of bonding agent. 2. Acid etched surfaces showed the lowest bond strength. Bond strengths obtained from experimental groups including acid etching were lower than those obtained from same kinds of experimental groups without acid etching. 3. Simple method of the coating of bonding agent produced the slightly increased bond strength on chemical curing composite and reduced bond strength on light curing composite. 4. Bonding surfaces of chemical curing composite resin showed slightly higher bond strengths than light curing composite resin, however significant differences were not confirmed statistically. 5. More significant irregular surfaces were created by sand-blasting method than acid etching method. 6. A principal component of fillers of both resins was silicon. Acid etching method produced the seperations and degradations of fillers, these were significant on light curing composite resins which containing barium fillers.

근관충전용 글라스 아이오노머 시멘트의 근단 폐쇄성과 치질 적합성에 관한 연구

김현아,최기운,민병순,최호영,박상진 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to compare the apical leakage and adaptation to the dentinal wall of a glass ionomer root canal cement(Ketac-Endo) with those of AH-26 and Tubli-Seal. 102 single-rooted teeth were insrumented with step-back technique and randomly divided into four groups according to kinds of root canal cements: Ketac-Endo, AH-26, Tubli-Seal, and no root canal cement. Four experimental groups were as follows; Group 1 Filling with gutta-percha and Ketac-Endo Group 2 Filling with gutta-percha and .AH-26 Group 3 Filling with gutta-percha and Tubli-Seal Group 4 Filling with gutta-percha without root canal cement All the specimens were obturated with lateral condensation technique, and stored in 100% humidity for 4 days. 20 teeth in each group were placed in centrifuge tube filled with India ink, and then centrifuged for 20 minutes at 3,000 rpm. And they were evaluated for linear dye leakage using a stereoscope and analyzed by one-way ANOVA. 22 teeth were split longitudinally and evaluated the adaptation to the dentinal wall using scanning electron mrcroscope(4 teeth in each group) and stereoscope(2 teeth in 1, 2, 3 group). The obtained results were as follows ; 1. In case of not used root canal cement(Group 4), there was showed much degree of dye penetration comparerd with used root canal cements (Group 1, 2, 3)(P<0.01). 2. In the case of used root canal cements(Group 1, 2, 3), there was no significant difference in linear leakage in groups(P>0.01). 3. In the case of used root canal cements(Group 1, 2, 3), there was showed intimate adaptation to the dentinal wall independent on kinds of root canal cements. 4. Fractured surfaces indicated failure of cohesion in Ketac-Endo, and failure of adhesion to dentine in AH-26 and Tubli-Seal. 5. According to similar apical leakage and adaptability to the dentinal wall to another root canal cements, it is possible to be used glass-ionomer cement as root canal cement.