아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

무증상 갑상선기능저하증 환자에서 Thyroxine 치료에 따른 혈청 지질 및 아포지단백의 변화

조보연,신찬수,김원배,고창순,김성연,박혜영,박건상,이홍규,박형규,김숙경 대한내분비학회 1996 Endocrinology and metabolism Vol.11 No.1

Background: Subclinical hypothyroidism(SCH) is a common biochemical abnormality which can be found in routine screening tests of thyroid function. We are increasingly faced with the question of whether its an indication for thyroxine replacement therapy. The effect of thyroxine replacement on lipid profile in SCH has aroused a great interest because of an association of overt hypothyroidism(OVH) with hyperlipidemia and increased risk of coronary artery disease. Method: We prospectively evaluated the changes in lipids and apoproteins before and after thyroxine replacement therapy in 23 patients with SCH and in 37 patients with OVH. We measured serum total cholesterol and triglyceride using autoanalyzer, high density lipoprotein(HDL) chole-sterol by dextran sulfate method, Apo A1 and Apo B by immunonephelometric assay. Results: Thyroxine replacement therapy significantly decreased total cholesterol, low density lipoprotein(LDL) cholesterol and apo B levels, but did not affect the level of triglyceride, HDL cholesterol or apo AI in patients with OVH. In SCH, thyroxine replacement therapy with the doses to normalize serum TSH concentrations also decreased significantly the level of cholesterol and LDL cholesterol albeit apo B levels did not change. Moreover, in most of patients with OVH (11 of 12) and in all of patients with SCH(5 of 5) who had had hyperchlesterolemia before treatment, thyroxine replament normalized their cholesterol and LDL cholesterol levels. Conclusion: In regard to the beneficial changes in blood lipid levels, patients with SCH should be treated, especially in cases who have other risk factors for the development of atherosclerosis. If thyroxine replacement only will reduce the incidence of coronary artery disease in SCH remains to be elucidated by long-term prospective studies(J Kor Soc Endocrinol 11:41-51, 1996).

분뇨처리장의 공정개선에 의한 처리효율 향상에 관한 연구

이찬원,김승현,김창수,문성원,전홍표,윤종섭 한국환경과학회 2004 한국환경과학회지 Vol.13 No.6

There is a need to improve the efficiency of the existing sanitary treatment facilities, because the effluent standard becomes more stricter and septic sludge increased. Thus, operating processes of sanitary treatment system in M city changed with installation of additional facilities. Process modifications were as follows: Dilution water was added to the next process after primary aeration tank. Some secondary sedimentation sludge was recycled to primary aerator so that most of the organics were stabilized in primary aeration tank under automatic control of dissolved oxygen. The line of effluent from dewatering process flowing to the activated sludge tank was changed to the primary aerator. The primary sedimentation sludge line was linked to a thickener. Polymer was added to the activated sludge tank. The effluent of primary aerator and aerobic digester was recycled from the 5th to the 1st sector. As consequencies of above process modifications, the improvement of removal efficiency was achieved as BOD 54%, COD 42%, SS 61%, T-N 39%, and T-P 12%, respectively.

金元時代 醫學의 學術的 特徵

尹暢烈,洪元植 慶熙大學校韓醫科大學韓醫學硏究所 1984 慶熙韓醫大論文集 Vol.7 No.-

大豆에 있어서 Gamma ray 感受性의 品種間 差異

Ki Chang Hong(洪基昶),Sin Won Kang(姜信元) 한국육종학회 1970 한국육종학회지 Vol.2 No.1

大豆 放射線 育種의 한가지 基礎硏究로서 Gamma ray에 對한 感受性의 品種間 差에 對하여 試驗한 바 그 結果를 要約하면 다음과 같다. (1) Gamma ray 照射에 따라서 高線量에서는 發芽率이 大體로 抑制傾向을 보인 것이 많았다. Hill 만을 全線量(15~60kR)에서 發芽率이 增大되었다. (2) 全品種이 Gamma ray 照射에 依해 發芽期間이 延長되었으나 忠北白과 益山만은 低線量에서는 도리어 短縮되는 傾向을 表示하고 있다. (3) 上胚軸長은 全體的으로 減少 傾向을 보였으나 忠北白과 益山은 低線量에서는 도리어 增大傾向을 보였다. (4) 下胚軸長도 全體的으로 減少 傾向을 보였으나 Hill만은 全線量에서, 益山을 除한 全品種이 15kR에서 增大傾向을 보였다. (5) 葉面指數는 全品種이 全線量에 增大되었다. (6) 莖長은 益山과 Hill에서는 15~35kR 에서 增大되고 45kR 以上에서 減少되었으며 基他 品種은 全線量에서 減少되었다. (7) 葉長은 거의 全照射區에서 減少되었으나 金豆益山 및 Hill의 15 kR 增大되는 傾向이 있었다. (8) Gamma ray 照射에 依하여 12種類 以上의 畸型葉이 相當한 頻度로 出現하였다. (9) 初期부터 여러個의 側枝가 一齊히 發育하여 正常個體보다 生育이 極히 旺盛한 異常 個體도 發見되었다. In order to find out the varietal differences of radiation sensitivity in five soybean varieties, Chung-buk-baek, Yuk-u #3, Keum-du, lk-san, Chang-dan-baek-mok and Hill were treated with 15, 25, 35, 45, and 60 kR of gamma ray. The results gained are summarized as follows; (1) The germination percentage decreased with an increase of gamma ray dosage except the slight stimulation at lower doses in most of the varieties. But Hill was stimulated from all doses. (2) The length of time for germination was prolonged by the gamma ray irradiation in four varieties, while Chung-buk-back and Ik-san were shortened in low dose. (3) The length of epicotyl was reduced in four varieties, the other varieties, Chungbuk-baek and Ik-san were increased in lower doses. (4) The length of hypocotyl was reduced with increasing dosage in five varieties except Hill, which was increased with all doses, while all varieties, except Ik-san were increased at 15 kR. (5) The leaf index was increased proportionally to high dosage. (6) The length of stem was increased between 15 kR and 35 kR in Ik-san and Hill, but reduced in all doses in the other four varieties. (7) in general, the length of leaf was decreased from all doses, but it was increased at the 15 kR level in Keum-do, Ik-san and Hill. (8) More than twelve kinds of abnormal leaf types resulted from various dose levels. (9) More vigorous plants of a bushy type were found in the experiment.

요추부 유합수술 후 가동인접분절의 후기변화

김홍태,강도원,유찬훈,정재호,장세앙 대한척추외과학회 1996 대한척추외과학회지 Vol.3 No.1

The spinal fusion in a lumbar region may influence biomechanically in the remained mobile segment to take over the lost motions Therefore the stress concentration on the adjacent segments may accelerate the degenerative changes, and then various late changes could occur. The aim of this study was to evaluate the late changes occuring in the adjacent segments to lumbar fusions. A retrospective review of radiographs and medical records was undertaken for 67 consecutive patients who had undergone various fusions in the lumbar region for various pathologic conditions. Included in this study were the patients who had performed active daily livings without any significant pain or disability after fusion and followed for a minimum of five years(up to 18 years with a mean of 8.3 years). The results of this study were as follows : The instability, disc narrowing, spinal stenosis, vertebral slipping, or isthmic defect were found in the adjacent segments to fusions in the patients who were followed longer, in L4-5 segment and above segment of fusion, and in the adjacent segments where a degenerative changes existed before surgery and where the angular motion increased considerably in a few years after fusion. The symptoms of these patients were responded well with conservative theraphy and no patient needed any surgical theraphy during these follow-up periods.

HPV에 감염된 자궁경부 상피세포의 Keratin 아형 변화에 관한 연구

기근홍,이영미,장원재,임용,임성철,김창원,전호종 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The human papillomavirus (HPV) are wide spread in the world, causing proliferation of epidermal and mucosal surface. Certain papillomaviral types are oncogenic in vivo and in vitro. HPV DNA has been detected in most of cervical lesions such as condyloma acuminata, dysplasias, and invasive carcinomas. The materials for this study consisted of 5 dysplasias. 5 squamous cell carcinomas, and 5 exocervix for control, that resected for definite treatment of uterine disease at Chosen University Hospital from January, 1991 to December, 1993. All cases were performed of in situ hybridization for HPV DNA type 6/11, 16, and 18. And also immununohistochemical stain for panel of monoclonal cytokeratin (CK) antibodies were done. The result obtained is as fallows : 1. HPV DNA type 6/11 are detected in all dysplasias. HPV DNA type 16 and 18 are detected in squamous cell carcinomas. 2. Stratifed squamous epithelium of exocervix are positive staining for CK 1, CK 5/6, CK 8, CK 13, CK 14, and CK 19. HPV DNA type 6/11 infected cells are positive for CK 1, CK 13, CK 14, CK 17, CK 18, and CK 19. HPV DNA type 16/18 infected cells are positive for CK 1, CK 5/6, CK 10, CK 13, CK 14, CK 17, CK 18, and CK 19. In conclusion, HPV infection of uterine cervix is associated with change of normal cytokeratin expression pattern. Dysplastic cells are changed of CK 5/6, CK 8, CK 17 and CK18. Squamous cell carcinoma cells are changed of CK 8, CK 17, and CK 18.

R&D 투자 영향평가 기반 구축 및 시범분석

황석원(Seog-Won, Hwang),오승환(Seung-Hwan, Oh),우청원(Ceung-Won, Woo),장필성(Pil-Seoung, Jang),홍사균(Sa-Gyun, Hong),강희종(Hee-Jong, Kang),최창택(Chang-Taek, Choi),김기환(Gi-Hwan, Kim),이재진(Jae-Jin, Lee),김지훈(Ji-Hoon, Kim) 과학기술정책연구원 2016 정책연구 Vol.- No.-

This study is a late study of “An Exploratory Study on STI policy impact assessment”. In the research of 2015, in principle, it is necessary to evaluate not only R&D investment but also general policies such as various promotion systems, standards and regulations by putting the term “policy impact assessment” in front of it We emphasized the points. However, it is practically very difficultto collect and analyze detailed data of all STI policies. In addition, it is also restricted to allow policy researchers to access data within a certain range. Therefore, this study focuses on the impact assessment of R&D investment, which accounts for the largest percentage of STI policies. In this research, the impact assessment system of R&D investment is analyzed by classifying it into evaluation of economic impact and social impact assessment. Also, the evaluation of economic impact was analyzed separating into a micro viewpoint and a macro viewpoint. First, The analysis of the economic impact assessment of the micro viewpoint relates to how the government’s R&D investment affects enterprises. Specifically, the main analysis target was the employment and investment of companies, the impact on sales and profits. Second, The Analysis for macroeconomic impact assessment on the economy is related to the influence of government’s R&D investment such as national economic growth, employment etc. We also analyzed the spillover effect by industry according to R&D investment. Third, The social impact assessment is to analyze how R&D investment has impacted technology-based social change. This study is a very difficult attempt. However, we analyzed from the point of view of the consumer considering all social change areas as much as possible. The results of the study are summarized as follows. First, the study of economic impact evaluation shows the effect of R&D investment and it can be used as a basis to improve the efficiency and goal of R&D investment in future sectors such as employment, economic growth and investment. Second, through research on social impact assessment, government R&D investment has focused on specific areas in terms of improving the quality of life and social development of people, and confirmed that most social sectors are neglected. In addition, we proposed policy priorities for specific social change indicators and social sectors and proposed long-term R&D investment strategies. Finally, this study is expected to contribute to the establishment of future strategy of private enterprise through the measurement of social change index, the social influence of private research and development, and the influence of technology shock from abroad.

전립선암의 조기진단을 위한 염색체 이상에 관한 연구 : Fluorescence In Situ Hybridizatioan법을 중심으로

박종윤,박정덕,홍연표,장임원 중앙대학교 의과대학 의과학연구소 1994 中央醫大誌 Vol.19 No.1

To detect the numerical changes of the chromosome 13 and 17, containing tumor suppressor genes (retinoblastoma gene and p53) in normal tissue, intraepithelial neoplasm and carcinoma of the prostate, the Fluorescence In Situ Hybridization technique with paraffin embedded tissue was done. Materials observed in this study consisted of 14 cases of lacalized carcinoma(TB1NOMO) and 1 case of locally infiltrating cancer(TC1NOMO)> The chromosomal number changes were counted at normal tissue, intraepithelial neoplasm and neoplastic gland at the same radical prostatectomy specimen. From the 4 patients, studied prospectively, fresh cancer tissue and peripheral whole blood were collected for the primary culture of the cancer cells and lymphocytic culture. There cultured cells were used fro karyotyping. The results were as follows : 1. There were no significant chromosomal number changes between 13 and 17 in each group. 2. The normal non-neoplastice duct, intraepithelial neoplasm and neoplastic gland of experimental group showed no significant difference in chromosomal change. 3. There were significant changes more than 30% in chromosomal numbers between control Y chromosome and experimental chromosome 13 and 17 in each group. 4. The karyotyping on primary cultured prostate cancer cells and lymphocyte showed significant difference in chromosomal number in spite of the same host, representing local chromosomal changes.

6-OHDA가 흰쥐 등쪽솔기핵내 도파민성 신경세포와 별아교세포에 미치는 영향

홍은석(Eun Seog Hong),남성안(Seong Ahn Nam),조승묵(Seung Mook Jo),강태천(Tae-Cheon Kang),원무호(Moo Ho Won),최창도(Chang Do Choi) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.4

신경독성물질인 6-hydroxydopamine (6-OHDA)이 중추신경계통 내 도파민성 신경세포에 미치는 영향과 이에 따른 별아교 세포들의 반응을 형태학적으로 규명하고자 본 연구를 시행하였다. 흰쥐의 가쪽뇌실에 6-OHDA를 투여한 후 면역조직화학 염색을 시행하여 광학현미경 하에서 등쪽솔기핵 내 도파민성 신경세포에 나타나는 퇴행성변화와 주위 별아교세포들의 반응양상 등을 경시적으로 제 3일, 제 5일, 제10일 및 제20일에서 관찰하였던 바 아래와 같은 결과를 얻었다. 6-OHDA에 의한 도파민성 신경세포의 퇴행성변화는 약물투여후 제 3일군에서 세포돌기가 팽대되고, 제 5일군과 제 10일군에서는 세포돌기의 소실과 함께 세포체가 위축되었고, 제20일군에서는 세포체가 소실되었다. 또한 6-OHDA에 의해 도파민성 신경세포들의 손상이 진행되면서 별아교세포들의 반응양상은 제 5일에서 대조군에 비해 급격하게 증가하였으며, 제20일에 이르러 최고값을 보였다. 이상의 결과로부터 6-OHDA가 중추신경계통 내 도파민성 신경세포들에 선택적이고 강한 세포독성을 유발하여 퇴행성 변화가 진행되고, 세포 손상이 시간 경과에 따라 진행되면서 주위의 별아교세포들이 지속적으로 증가된 활동을 보인다는 사실을 알게 되었다. This study was designed to clarify the cytotoxic effects of 6-hydroxydopamine (6-OHDA) on the dopaminergic neurons and astrocytes in the dorsal raphe nucleus (DRN), and to investigate neurodegenerative changes by immunohistochemistry. Adult male rats (Sprague-Dawley strain) weighing from 250 to 350 g were used as experimental animals. 6-OHDA (100 μg dissolved in 0.1% ascorbic acid) was injected into the lateral ventricle of the rat brain with the Hamilton syringe. The control rats were treated with the similar volume of 0.1 % ascorbic acid. The rats were sacrificed at the 3rd, 5th, 10th and 20th day, respectively, after the injection of 6-OHDA. The cytotoxicity of 6-OHDA resulted in severe neurodegeneration of the dopaminergic neurons in the DRN. In the 3rd day, the dopaminergic fibers were dilated. In the 5th and 10th days, the dopaminergic fibers were depleted, and dopaminergic cell bodies were shrunken. In the 20th day, the dopaminergic cell bodies were almost completely disappeared. Astroglial reactions induced by 6-OHDA were also observed in the DRN. In the 5th day, astrocytes were significantly increased as compared with that of the control value. The value were reached at its maximum by the 20th day. Based on the present results, it suggests that 6-OHDA may act as a specific neurotoxin to dopaminergic neurons in the DRN, and induce severe neurodegenerative changes. Also, it suggests that the astroglial reaction in the DRN is gradually activated during the neurodegerative changes