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Bingkui Jin,Girim Park,Youngmi Choi,Jaejong Nho,Beunggu Son,Younghoon Park 한국원예학회 2017 원예과학기술지 Vol.35 No.1
Modern watermelon cultivars (Citrullus lanatus [Thunb.] Matsum.& Nakai var. lanatus ) have fruits with diverse phenotypes, including fruit shape, rind patterns, and flesh color. Molecular markers enable efficient selection of plants harboring desirable phenotypes. In the present study, publicly available DNA markers tightly linked to fruit shape, rind stripe pattern, and flesh color were evaluated using 85 watermelon accessions with diverse fruit phenotypes. For fruit shape, the dCAPS SUN - Cla011257 marker revealed an 81% of marker - trait match for accessions with elongated or round fruits. For rind stripe pattern, the SCAR wsb6-11marker was effective for selecting Jubilee-type rind pattern from other rind patterns. For flesh color, the Clcyb.600 and Lcyb markers derived from a mutation in the Lycopene β - cyclase (Lcyb) gene, were effective at selecting red or yellow flesh. Forty-eight accessions possessing diverse fruit - related traits were selected as a reference array and their genetic relationships assessed using 16 SSR markers. At a coefficient of 0.11, the 48 accessions grouped into two major clades: Clade I and Clade II. Clade I subdivided further into subclades I - 1 and I - 2 at a coefficient of 0.39. All accessions with colored flesh were classified into Clade I, whereas those with white - flesh were classified into Clade II. Differences in fruit traits between subclades I - 1 and I - 2 were observed for rind pattern and fruit color; a majority of the accessions with Crimson-type striped or non-striped rind were grouped together in subclade I - 1, while most accessions in subclade I - 2 had a Jubilee - type rind stripe pattern. These results imply that reference array watermelon accessions possess distinguishable genetic structure based on rind stripe pattern. However, no significant grouping pattern was observed based on other fruit-related traits.
Bingkui Jin,Joonyup Kim,Jaemin Jung,Daeun Kim,Younghoon Park 한국원예학회 2018 원예과학기술지 Vol.36 No.1
The SUN gene is responsible for an elongated fruit shape in tomato and belongs to the IQ domain (IQD) gene family, which is involved in growth and development in plants. In the present study, IQD gene homologs were evaluated for their roles in determining fruit shape in cucurbit crops. A total of 151 IQD homologs and their chromosomal locations in Arabidopsis, tomato, and three cucurbit species, watermelon, melon, and cucumber, were compared based on their genomic information. A phylogenetic dendrogram of these IQD homologs revealed putative orthologous and paralogous relationships among these genes, and showed that previously reported candidate fruit shape IQD genes in watermelon (ClSUN8), melon (CmSUN14), and cucumber (CsSUN2) were clustered under the same node with a similarity coefficient of 0.97. A comparison of the physical locations of the IQD homologs with fruit shape QTLs in genetic maps indicated that ClSUN8, CmSUN14, and CsSUN2 were co-localized with the major QTLs for the fruit shape index (FSI). This co-localization further indicated that minor QTLs for FSI were also associated with the IQD gene family. We used early developmental stages of immature fruits to conduct a morphological assay and characterize ClSUN8 in watermelon. Histological analysis indicated that elongated or round fruit shape is determined at the early stage of ovary formation, at least earlier than -4 days after fertilization (-4 DAF), and fruit elongation is due to increased cell division in the longitudinal direction. Quantitative RT-PCR showed that the highest expression of ClSUN8 occurred at -4 days after fertilization and gradually decreased; however, there was no direct relationship between the gene expression level and fruit shape. RACE-PCR revealed a non-synonymous SNP between the ClSUN8 alleles of elongated- and round-fruited watermelon accessions, suggesting that the SNP might be a causative mutation affecting fruit shape.
Bingkui Jin,Junewoo Lee,Seungan Kweon,Youngwoo Cho,Youngmi Choi,Sung Joong Lee,박영훈 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.3
Fourteen watermelon cultivars with diff erent fruit fl esh colors (red, salmon yellow, orange, and canary yellow) were analyzedfor carotenoid contents (prolycopene, lycopene, β-carotene, ζ-carotene, and neoxanthin). Genes encoding the carotenoidbiosynthesis enzymes carotenoid isomerase (encoded by CRTISO ), which catalyzes the isomerization of prolycopene tolycopene, and β-carotene hydroxylase ( CHYB ), which catalyzes the conversion of β-carotene to xanthophyll, were alsoanalyzed. High-performance liquid chromatography showed that the salmon yellow and orange fl esh accumulated eitherprolycopene (orange-P fl esh) or β-carotene (orange-β fl esh), whereas lycopene and neoxanthin were the main carotenoids inthe red and canary yellow fl esh, respectively. Quantitative real-time polymerase chain reaction indicated that CRTISO andCHYB were mainly expressed during fruit maturation, regardless of the fl esh color, and there was no signifi cant associationbetween diff erential gene expression and fl esh color. Importantly, transcript sequencing revealed a non-synonymous singlenucleotidemismatch (T > C 1976 ) in exon 13 of CRTISO between orange-P-fl eshed and other cultivars, suggesting CRTISO asa candidate gene for high prolycopene accumulation. However, in β-carotene-accumulating cultivars, there were no mutationsin CHYB transcripts. A cleaved amplifi ed polymorphic sequence marker was developed for T > C 1976 , and its applicabilityfor marker-assisted selection of orange-P fl esh was validated in 105 watermelon accessions.
Kim, Daeun,Jin, Bingkui,Je, Byoung Il,Choi, Youngmi,Kim, Byung Sup,Jung, Hee-Jeong,Nou, Ill-Sup,Park, Younghoon,Archambault, A. Canadian Science Publishing 2018 Genome Vol.61 No.10
<P> Reductions in growth and quality due to powdery mildew (PM) disease cause significant economic losses in tomato production. Oidium neolycopersici was identified as the fungal species responsible for tomato PM disease in South Korea in the present study, based on morphological and internal transcribed spacer DNA sequence analyses of PM samples collected from two remote regions (Muju and Miryang). The genes involved in resistance to this pathogen in the tomato accession ‘KNU-12’ (Solanum lycopersicum var. cerasiforme) were evaluated, and the inheritance of PM resistance in ‘KNU-12’ was found to be conferred via simple Mendelian inheritance of a mutant allele of the PM susceptibility locus Ol-2 (SlMlo1). Full-length cDNA analysis of this newly identified mutant allele (Slmlo1.1) showed that a 1-bp deletion in its coding region led to a frameshift mutation possibly resulting in SlMlo1 loss-of-function. An alternatively spliced transcript of Slmlo1.1 was observed in the cDNA sequences of ‘KNU-12’, but its direct influence on PM resistance is unclear. A derived cleaved amplified polymorphic sequence (dCAPS) and a high-resolution melting (HRM) marker were developed based on the 1-bp deletion in Slmlo1.1, and could be used for efficient marker-assisted selection (MAS) using ‘KNU-12’ as the source for durable and broad-spectrum resistance to PM. </P>
정재민,김준엽,Bingkui Jin,최영미,홍창오,이현호,최영환,강점순,박영훈 한국원예학회 2017 원예과학기술지 Vol.35 No.3
Bloomless cucumber fruits are commercially produced by grafting onto the pumpkin stocks(Cucurbita moschata ) to restricted silicon (SiO2) absorption. Inhibition of silicon absorptionin bloomless stocks is conferred by a mutant allele of the CmLsi1 homologous to Lsi1 in rice. In this study, we characterized the Lsi1 homologs in pumpkin (C. moschata ) and its coldtolerantwild relative C. ficifolia (‘Heukjong’) in order to develop a DNA marker for selectinga bloomless trait and to establish the molecular basis for breeding bloomless stock cultivarsof C. ficifolia . A Cleaved amplified polymorphic sequence (CAPS) marker (CM1-CAPS) wasdesigned based on a non-sysnonymous single nucleotide polymorphism (SNP, C>T) of theCmLsi1 mutant-type allele, and its applicability for Marker-assisted selection (MAS) wasconfirmed by evaluating three bloom and five bloomless pumpkin stock cultivars. QuantitativeRT-PCR of the CmLsi1 for these stock cultivers implied that expression level of the CmLsi1gene does not appear to be associated with the bloom/bloomless trait and may differ dependingon plant species and tissues. A full length cDNA of the Lsi1 homolog [named CfLsi1 (B+)]of ‘Heukjong’ (C. ficifolia ), was cloned and sequence comparison between CmLsi1 (B+) andCfLsi1 (B+) revealed that there exists total 24 SNPs, of which three were non-synonymous. Phylogenetic analysis of CfLsi1 (B+) and Lsi1 homologs further revealed that CfLsi1 (B+) isclosesly related to Nodulin 26-like intrinsic proteins (NIPs) and most similar to CpNIP1 of C. pepo than C. moschata .
Jung, Jaemin,Kim, Joonyup,Jin, Bingkui,Choi, Youngmi,Hong, Chang Oh,Lee, Hyun Ho,Choi, Youngwhan,Kang, Jumsoon,Park, Younghoon Korean Society of Horticultural Science 2017 원예과학기술지 Vol.35 No.3
Bloomless cucumber fruits are commercially produced by grafting onto the pumpkin stocks (Cucurbita moschata) to restricted silicon ($SiO_2$) absorption. Inhibition of silicon absorption in bloomless stocks is conferred by a mutant allele of the CmLsi1 homologous to Lsi1 in rice. In this study, we characterized the Lsi1 homologs in pumpkin (C. moschata) and its cold-tolerant wild relative C. ficifolia ('Heukjong') in order to develop a DNA marker for selecting a bloomless trait and to establish the molecular basis for breeding bloomless stock cultivars of C. ficifolia. A Cleaved amplified polymorphic sequence (CAPS) marker (CM1-CAPS) was designed based on a non-sysnonymous single nucleotide polymorphism (SNP, C>T) of the CmLsi1 mutant-type allele, and its applicability for Marker-assisted selection (MAS) was confirmed by evaluating three bloom and five bloomless pumpkin stock cultivars. Quantitative RT-PCR of the CmLsi1 for these stock cultivers implied that expression level of the CmLsi1 gene does not appear to be associated with the bloom/bloomless trait and may differ depending on plant species and tissues. A full length cDNA of the Lsi1 homolog [named CfLsi1($B^+$)] of 'Heukjong' (C. ficifolia), was cloned and sequence comparison between CmLsi1($B^+$) and CfLsi1($B^+$) revealed that there exists total 24 SNPs, of which three were non-synonymous. Phylogenetic analysis of CfLsi1($B^+$) and Lsi1 homologs further revealed that CfLsi1($B^+$) is closesly related to Nodulin 26-like intrinsic proteins (NIPs) and most similar to CpNIP1 of C. pepo than C. moschata.
Jaemin Jung,Joonyup Kim,Bingkui Jin,Youngmi Choi,Chang Oh Hong,Hyun Ho Lee,Youngwhan Choi,Jumsoon Kang,Younghoon Park 한국원예학회 2017 원예과학기술지 Vol.35 No.3
Bloomless cucumber fruits are commercially produced by grafting onto the pumpkin stocks (Cucurbita moschata ) to restricted silicon (SiO₂) absorption. Inhibition of silicon absorption in bloomless stocks is conferred by a mutant allele of the CmLsi1 homologous to Lsi1 in rice. In this study, we characterized the Lsi1 homologs in pumpkin (C. moschata ) and its coldtolerant wild relative C. ficifolia (‘Heukjong’) in order to develop a DNA marker for selecting a bloomless trait and to establish the molecular basis for breeding bloomless stock cultivars of C. ficifolia . A Cleaved amplified polymorphic sequence (CAPS) marker (CM1-CAPS) was designed based on a non-sysnonymous single nucleotide polymorphism (SNP, C>T) of the CmLsi1 mutant-type allele, and its applicability for Marker-assisted selection (MAS) was confirmed by evaluating three bloom and five bloomless pumpkin stock cultivars. Quantitative RT-PCR of the CmLsi1 for these stock cultivers implied that expression level of the CmLsi1 gene does not appear to be associated with the bloom/bloomless trait and may differ depending on plant species and tissues. A full length cDNA of the Lsi1 homolog [named CfLsi1 (B⁺)] of ‘Heukjong’ (C. ficifolia ), was cloned and sequence comparison between CmLsi1 (B⁺) and CfLsi1 (B⁺) revealed that there exists total 24 SNPs, of which three were non-synonymous. Phylogenetic analysis of CfLsi1 (B⁺) and Lsi1 homologs further revealed that CfLsi1 (B⁺) is closesly related to Nodulin 26-like intrinsic proteins (NIPs) and most similar to CpNIP1 of C. pepo than C. moschata.