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Bingkui Jin,Junewoo Lee,Seungan Kweon,Youngwoo Cho,Youngmi Choi,Sung Joong Lee,박영훈 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.3
Fourteen watermelon cultivars with diff erent fruit fl esh colors (red, salmon yellow, orange, and canary yellow) were analyzedfor carotenoid contents (prolycopene, lycopene, β-carotene, ζ-carotene, and neoxanthin). Genes encoding the carotenoidbiosynthesis enzymes carotenoid isomerase (encoded by CRTISO ), which catalyzes the isomerization of prolycopene tolycopene, and β-carotene hydroxylase ( CHYB ), which catalyzes the conversion of β-carotene to xanthophyll, were alsoanalyzed. High-performance liquid chromatography showed that the salmon yellow and orange fl esh accumulated eitherprolycopene (orange-P fl esh) or β-carotene (orange-β fl esh), whereas lycopene and neoxanthin were the main carotenoids inthe red and canary yellow fl esh, respectively. Quantitative real-time polymerase chain reaction indicated that CRTISO andCHYB were mainly expressed during fruit maturation, regardless of the fl esh color, and there was no signifi cant associationbetween diff erential gene expression and fl esh color. Importantly, transcript sequencing revealed a non-synonymous singlenucleotidemismatch (T > C 1976 ) in exon 13 of CRTISO between orange-P-fl eshed and other cultivars, suggesting CRTISO asa candidate gene for high prolycopene accumulation. However, in β-carotene-accumulating cultivars, there were no mutationsin CHYB transcripts. A cleaved amplifi ed polymorphic sequence marker was developed for T > C 1976 , and its applicabilityfor marker-assisted selection of orange-P fl esh was validated in 105 watermelon accessions.