http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Joonyup,Yang, Ronghui,Chang, Caren,Park, Younghoon,Tucker, Mark L Oxford University Press 2018 Journal of experimental botany Vol.69 No.12
<▼1><P>A root-knot nematode gene (<I>MiIDL1</I>) produces a peptide that mimics the Arabidopsis IDA signaling peptide that regulates cell separation events including abscission and lateral root emergence.</P></▼1><▼2><P><B>Abstract</B></P><P>INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) is a signaling peptide that regulates cell separation in Arabidopsis including floral organ abscission and lateral root emergence. IDA is highly conserved in dicotyledonous flowering plant genomes. <I>IDA-like</I> sequences were also found in the genomic sequences of root-knot nematodes, <I>Meloidogyne</I> spp., which are globally deleterious pathogens of agriculturally important plants, but the role of these genes is unknown. Exogenous treatment of the Arabidopsis <I>ida</I> mutant with synthetic peptide identical to the <I>M. incognita</I> IDA-like 1 (MiIDL1) protein sequence minus its N-terminal signal peptide recovered both the abscission and root architecture defects. Constitutive expression of the full-length <I>MiIDL1</I> open reading frame in the <I>ida</I> mutant substantially recovered the delayed floral organ abscission phenotype whereas transformants expressing a construct missing the MiIDL1 signal peptide retained the delayed abscission phenotype. Importantly, wild-type Arabidopsis plants harboring an <I>MiIDL1</I>-RNAi construct and infected with nematodes had approximately 40% fewer galls per root than control plants. Thus, the <I>MiIDL1</I> gene produces a functional IDA mimic that appears to play a role in successful gall development on Arabidopsis roots.</P></▼2>
Wu, Jingni,Wang, Yiming,Kim, Sang Gon,Jung, Ki‐,Hong,Gupta, Ravi,Kim, Joonyup,Park, Younghoon,Kang, Kyu Young,Kim, Sun Tae Blackwell Publishing Ltd 2017 Physiologia plantarum Vol.161 No.2
<P>Chitinases belong to a conserved protein family and play multiple roles in defense, development and growth regulation in plants. Here, we identified a secreted chitinase‐like protein, OsCLP, which functions in rice growth. A T‐DNA insertion mutant of OsCLP (<I>osclp</I>) showed significant retardation of root and shoot growth. A comparative proteomic analysis was carried out using root tissue of wild‐type and the <I>osclp</I> mutant to understand the OsCLP‐mediated rice growth retardation. Results obtained revealed that proteins related to glycolysis (phosphoglycerate kinase), stress adaption (chaperonin) and calcium signaling (calreticulin and CDPK1) were differentially regulated in <I>osclp</I> roots. Fura‐2 molecular probe staining, which is an intracellular calcium indicator, and inductively coupled plasma‐mass spectrometry (ICP‐MS) analysis suggested that the intracellular calcium content was significantly lower in roots of <I>osclp</I> as compared with the wild‐type. Exogenous application of Ca<SUP>2+</SUP> resulted in successful recovery of both primary and lateral root growth in <I>osclp</I>. Moreover, overexpression of <I>OsCLP</I> resulted in improved growth with modified seed shape and starch structure; however, the overall yield remained unaffected. Taken together, our results highlight the involvement of OsCLP in rice growth by regulating the intracellular calcium concentrations.</P>
26-Week Repeated Dose Oral Toxicity Study of KCHO-1 in Sprague-Dawley Rats
Yang, Muhack,Lee, Seongjin,Wang, Tingting,Cha, Eunhye,Jang, Jongwon,Kim, Dongwoung,Song, Bong-Keun,Son, Ilhong,Kim, Joonyup,Kang, Hyung Won,Kim, Sungchul KOREAN PHARMACOPUNCTURE INSTITUTE 2019 Journal of pharmacopuncture Vol.22 No.3
Objectives: KCHO-1(Mecasin), also called Gamijakyakgamchobuja-tang originally, is a combination of some traditional herbal medicines in East Asia. This medicine has been used mainly for alleviating neuropathic pains for centuries in Korean traditional medicine. KCHO-1 was developed to treat pain, joint contracture and muscular weakness in patients with amyotrophic lateral sclerosis. This study was carried out to investigate the chronic toxicity of KCHO-1 oral administration in rats for 26 weeks. Methods: Sprague-Dawely rats were divided into four groups and 10 rats were placed in the control group and the high-dose group, respectively. Group 1 was the control group and the remaining groups were the experimental groups. In the oral toxicity study, 500 mg/kg, 1,000 mg/kg, and 2,000 mg/kg of KCHO-1 were administered to the experimental group, and 10 ml/kg of sterile distilled water was administered to the control group. Survival rate, body weight, feed intake, clinical signs, and visual findings were examined. Urinalysis, ophthalmologic examination, necropsy, organ weight, hematologic examination, blood chemical examination and histopathologic examination were performed. Results: Mortality and toxicological lesions associated with the administration of test substance were not observed in all groups. Conclusion: NOAEL(No observed adverse effect level) of KCHO-1 is higher than 2000 mg/kg/day. And, the above findings suggest that treatment with KCHO-1 is relatively safe.