Affinity-Purification of Fibrinogenase with High Proteolytic Activity from Agkistrodon halys (Chinese) Venom

Ma, Biao,Zhan, Ying,Wu, Dan,Jia, Jianping,Xu, Wentao,Luo, Yunbo 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.9

To purify and characterize the fibrinogenase with high proteolytic activity from Agkistrodon halys (Chinese) Venom. Monoclonal antibodies against fibrinogenase were prepared and a novel affinity chromatography equipped with a monoclonal antibody against fibrinogenase was developed and applied for the purification of fibrinogenases. The purified fibrinogenase was identified by fibrinolytic activity assay, and antithrombosis activity assay. HPLC chromatography and SDS-PAGE analysis demonstrated the uniformity and purity of the purified fibrinogenase. In comparison with a conventional A-50 chromatography method, affinity-purified fibrinogenase showed higher activity ($3631\;U\;mg^{-1}$ vs $501\;U\;mg^{-1}$). In addition, the physiological activity of the fibrinogenase both in vitro and ex vivo showed the purified fibrinogenase can specifically degrade $\beta$-, $\gamma$-fibrinogen and has a high anti-thrombotic activity. In conclusion, the purified fibrinogenase by affinity column were shown to be homogeneous and showed a high and specific proteolytic activity against $\beta$-chains of fibrinogen molecules and antithrombosis activity.

Affinity-Purification of Fibrinogenase with High Proteolytic Activity from Agkistrodon halys (Chinese) Venom

Biao Ma,Ying Zhang,Dan Wu,Jianping Jia,Wentao Xu,Yunbo Luo 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.9

To purify and characterize the fibrinogenase with high proteolytic activity from Agkistrodon halys (Chinese) Venom. Monoclonal antibodies against fibrinogenase were prepared and a novel affinity chromatography equipped with a monoclonal antibody against fibrinogenase was developed and applied for the purification of fibrinogenases. The purified fibrinogenase was identified by fibrinolytic activity assay, and antithrombosis activity assay. HPLC chromatography and SDS-PAGE analysis demonstrated the uniformity and purity of the purified fibrinogenase. In comparison with a conventional A-50 chromatography method, affinity-purified fibrinogenase showed higher activity (3631 U mg-1 vs 501 U mg-1). In addition, the physiological activity of the fibrinogenase both in vitro and ex vivo showed the purified fibrinogenase can specifically degrade β-, γ-fibrinogen and has a high anti-thrombotic activity. In conclusion, the purified fibrinogenase by affinity column were shown to be homogeneous and showed a high and specific proteolytic activity against β-chains of fibrinogen molecules and antithrombosis activity.

Gearshift characteristics of a two-speed transmission using new electrically controlled toothed band brakes

Biao Ma,Weichen Lu,He Wang,Liang Yu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.12

In order to realize the miniaturization and electrification of heavy vehicles, this paper innovatively puts forward a two-speed transmission device equiped with electrically controlled toothed band brakes. The dynamic models of the planetary gear and toothed band brake are derived, and the shift strategy is established to achieve the optimal dynamic performance. Moreover, the gearshift characteristics under different working parameters are studied to verify its feasibility. The results demonstrate that the toothed band brake has a short braking time and contributes to good shift performance, thus has potential for application. The effect of synchronous speed and actuator speed on shift performance is also analyzed. The increase of both speeds is beneficial to speed up the shift time, but lead to increased shift impact. The application of such a brake offers the possibility of full-electric and lightweight for electric vehicles.

Aptamers Improve the Expression of a Human Granulocyte-Macrophage Colony Stimulating Factor in Transgenic Arabidopsis thaliana Seeds

( Biao Wang ),( Ming Ma ),( Tian Long Wu ) 한국식물학회 2007 Journal of Plant Biology Vol.50 No.1

Study on the Influence and Difference of Taijiquan and Five-animal Exercises on College Students' Ankle Muscle Strength

Nie, ShangQi,Ma, Biao,Song, Jeho The International Promotion Agency of Culture Tech 2020 International Journal of Advanced Culture Technolo Vol.8 No.3

The physique and health status of Chinese college students have received a generous concern in recent years. The physical condition of Chinese college students is not good and this phenomenon is especially obvious among female college students, which has been an disputable fact. This study starts from exercise intervention to study the concrete influence of taijiquan (a kind of traditional Chinese shadow boxing) and five-animal exercises on human body balance ability; the study uses experimental apparatus to measure the specific impact of exercise intervention on human body balance ability; meanwhile, the study also applies data analysis to longitudinally study whether the two studied sports items affect body balance, and whether there is difference in terms of the influence of two sports items on human body balance ability. The conclusions are as follows: firstly, 24-style taijiquan and five-animal exercises can improve ankle muscle strength, and the focus and degree of the improvement are different; secondly, five-animal exercises have a better improvement effect on the flexor and extensor muscles of ankle joint and 24-style taijiquan is in the second place. Moreover, compared with 24-style taijiquan, there is no significant difference in terms of the improvement caused by five-animal exercises; thirdly, 24-style taijiquan has a better effect on the improvement of internal rotation and evertors muscle strength. Compared with 24-style taijiquan, five-animal exercises have no significant difference in terms of the improvement degree.

Numerical studies on the dynamic characteristics of a wet multi-disc clutch during the disengaging process

Liangjie Zheng,Biao Ma,Man Chen,Liang Yu,Jiaqi Xue 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7

A comprehensive numerical model was proposed to investigate the disengaging process of a wet multi-disc clutch, which included the hydrodynamic lubrication, the micro elastic–plastic contact, the spline resistance, and the impact between the piston and clutch hub. The clutch disengaging process was divided into the boundary, mixed, and hydrodynamic lubrication stages to discuss the variations in friction pair gaps and friction torque. Moreover, the nonuniformity coefficient was employed to characterize the disengaging uniformity of friction pair gaps. During the disengaging process, the friction pair gaps increased slowly, increased rapidly, and stabilized after fluctuating. The increase in spline friction coefficient and the decline in pressure decrease rate dramatically deteriorated the disengaging uniformity; the decline in pressure decrease rate also significantly prolonged the disengaging time. This research demonstrated for the first time evidence for the dynamic characteristics of the clutch disengaging process.

Seismic performance of the concrete-encased CFST column to RC beam joints: Analytical study

Dan-Yang Ma,Lin-Hai Han,Xiao-Ling Zhao,Wei-Biao Yang 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.36 No.5

A finite element analysis (FEA) model is established to investigate the concrete-encased concrete-filled steel tubular (CFST) column to reinforced concrete (RC) beam joints under cyclic loading. The feasibility of the FEA model is verified by a set of test results, consisting of the failure modes, the exposed view of connections, the crack distributions and development, and the hysteretic relationships. The full-range analysis is conducted to investigate the stress and strain development process in the composite joint by using this FEA model. The internal force distributions of different components, as well as the deformation distributions, are analyzed under different failure modes. The proposed connections are investigated under dimensional and material parameters, and the proper constructional details of the connections are recommended. Parameters of the beam-column joints, including material strength, confinement factor, reinforcement ratio, diameter of steel tube to sectional width ratio, beam to column linear bending stiffness ratio and beam shear span ratio are evaluated. Furthermore, the key parameters affecting the failure modes and the corresponding parameters ranges are proposed in this paper.

Teacher receptivity to system-wide curriculum reform in the initiation stage: a Chinese perspective

Yun-peng Ma,Hong-biao Yin,Li-fang Tang,Li-yan Liu 서울대학교 교육연구소 2009 Asia Pacific Education Review Vol.10 No.3

Few studies deal with teachers' receptivity in the initiation stage of educational change, especially in a non-western cultural context like Mainland China. This study aims at investigating teachers' receptivity to the system-wide curriculum reform of the senior secondary education in the initiation stage and understanding the factors influencing teachers' receptivity in Mainland China. Questionnaire survey with open-ended question (n = 763) is employed to explore teachers' receptivity in four selected experimental provinces, i.e., the first group of provinces which are selected by Ministry of Education to implement the curriculum reform. Results indicate teachers have positive attitudes and behavioral intentions toward promoting the curriculum reform of senior secondary education, and they consider the reform is valuable but difficult to carry out. The existing theoretical model can explain teachers' behavioral intentions quite well, but its predicting ability to teachers' general attitudes is limited, which indicates some new variables that need to be considered, too. Implications of this study and suggestions for future research are also discussed in the article.

Temperature Response to Tensile Characteristics of the Hot Asphalt Mixtures

Wei Si,Ning Li,Biao Ma,Yu-xiang Tian,Xue-yan Zhou 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.4

Asphalt concrete is one type of the temperature sensitive mixtures, which is the most and broadest used pavement material in the world. Asphalt pavement suffers from the impact of temperature due to the temperature variation in different districts and environments. Facing temperature sensitivity, this paper uses Indirect Tensile Test (IDT) to analyze the tensile characters of asphalt mixture under different temperatures (-20°C, -10°C, 0°C, 15°C, 20°C, 45°C, and 60°C). The influence factors of temperature, loading rate, asphalt content, asphalt types, and mixtures gradation are evaluated. Piecewise linear model, logarithm linear model and S-logistic nonlinear model are applied to simulate the variation of flexural tensile characters with temperature change. Results show that temperature has significant influence on flexural tensile properties. Loading rates also has obvious influence on flexural tensile characteristics. With temperature changes, asphalt types have evident influence on mixture’s tensile characteristics, SBR modified asphalt has better performance at the lower temperature environment. Mixtures’ gradation has important effect on tensile strength and tensile strain as well, while due the similarity between two gradations, it is hard to precisely recommend which one is better. Asphalt content has evident influence on mixtures properties at the same temperature condition. Research results provide suggestions and applications for construction and performance of asphalt pavement. The results also indicate that IDT would be a practical option to evaluate the properties of asphalt mixture in all temperature ranges.

Multi Objective Optimization of Outer Rotor Wheel-Hub Permanent Magnet Synchronous Motor Based on Exact Analytical Method

Jing Yi-Yang,He Biao,Yang Xiang-Yu,Ma Zhi-Min 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1

Based on the exact analytical method, this paper calculates the no-load back electromotive force, air gap fux density, cogging torque, and electromagnetic torque of outer rotor wheel-hub permanent magnet synchronous motor (PMSM); the calculation results are in good agreement with those of the fnite element method, which verifes the accuracy of the exact analytical method for solving the outer rotor PMSM. On this basis, the second-generation non-dominated sorting genetic algorithm (NSGA-II) is applied to optimize the multiple structural parameters and performance of the motor more quickly. Compared with the initial scheme of the motor, the torque ripple and permanent magnet mass of the optimized scheme is decreased by a quarter; the average electromagnetic torque, efciency is also improved. Finally, the simulation and experimental results show that the proposed method is reasonable and efective.