Source Tracking Of Dissolved Organic Nitrogen At Molecular Level During Storm Events In An Agricultural Watershed

Most Shirina Begum(Begum Most Shirina),Jin Hur(허진),Seung Yoon Lee(이승윤) 대한환경공학회 2021 대한환경공학회 학술발표논문집 Vol.2021 No.11

L1 Recombinant Proteins of HPV Tested for Antibody Forming Using Sera of HPV Quadrivalent Vaccine

Begum Akuzum,김시내,Tam Thanh Nguyen,홍재우,이시영,김은혜,김주희,최여옥,전현정,이영민,김현우,손동현,김수현 대한면역학회 2018 Immune Network Vol.18 No.3

Virus-like particles (VLPs) derived from human papillomavirus (HPV) L1 capsid proteins were used for HPV quadrivalent recombinant vaccine. The HPV quadrivalent vaccine is administrated in a 3-dose regimen of initial injection followed by subsequent doses at 2 and 6 months to prevent cervical cancer, vulvar, and vaginal cancers. The type 6, 11, 16, or 18 of HPV infection is associated with precancerous lesions and genital warts in adolescents and young women. The HPV vaccine is composed of viral L1 capsid proteins are produced in eukaryotic expression systems and purified in the form of VLPs. Four different the L1 protein of 3 different subtypes of HPV: HPV11, HPV16, and HPV18 were expressed in Escherichia coli divided into 2 fragments as N- and C-terminal of each protein in order to examine the efficacy of HPV vaccine. Vaccinated sera failed to recognize N-terminal L1 HPV type 16 and type 18 by western blot while they detected N-terminal L1 protein of HPV type 11. Moreover, the recombinant C-terminal L1 proteins of type 16 was non-specifically recognized by the secondary antibody conjugated with horseradish peroxidase. This expression and purification system may provide simple method to obtain robust recombinant L1 protein of HPV subtypes to improve biochemical analysis of antigens with immunized sera.

Exfoliative Vaginal Cytology and Serum Progesterone during the Estrous Cycle of Indigenous Ewes in Bangladesh

Begum Fatema Zohara,Azizunnesa,Md. Faruk Islam,Md. Golam Shahi Alam,Farida Yeasmin Bari 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.2

A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, thepattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cyclelength and duration of estrus were 15.8±0.12 days and 31.1±0.57 h respectively. The exfoliated epithelial cells werecategorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages ofparabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell typeduring estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated byneutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to 1.6±0.07 ng/ml. The length ofdiestrus was 5∼10 days with a range of mean progesterone level of 1.6±0.07 to 2.8±0.11 ng/ml. Progesterone levelsincreased significantly (p<0.01) after Day 5 and maximum level was 2.8±0.11 ng/ml observed on Day 10 of theestrous cycle. Thereafter it dropped rapidly to basal level of 0.11±0.04 ng/ml on Day 0 (p<0.01). These results indicatethat the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine thereproductive stages of indigenous ewe

Effects of the plant extract YGF251 on growth performance, meat quality, relative organ weight, nutrient digestibility and blood profiles in broiler chickens: possible role of insulin-like growth factor 1

Begum, M.,Hossain, MM,Kim, IH INSTITUTE OF AGRICULTURAL FOOD INFORMATION 2014 VETERINARNI MEDICINA -PRAHA- Vol.59 No.9

<P>This study was aimed at evaluating the effects of different concentrations of the dietary plant extract supplement YGF251 (young growth factor) on growth performance, blood profiles, relative organ weight, nutrient digestibility and meat quality in broiler chickens. A total of 640 one-day-old male Arbor Acres broiler chickens with an average initial body weight of 40.25 &plusmn; 0.5 g were randomly allotted to one of four treatments lasting four weeks with 10 replicates per treatment and 16 chicks per replicate pen. Dietary treatments consisted of: CON (basal diet); YGF0.05 (CON + 0.05% YGF251); YGF0.1 (CON + 0.10% YGF251); YGF0.15 (CON + 0.15% YGF251). There were no statistical differences in body weight (BW), feed intake (FI) and feed conversion rate (FCR) throughout the whole experiment. However, broiler chickens fed with YGF0.1 diets had greater body weight gains (BWG) than chickens fed CON diets during eight to 28 days and zero to 28 days although the means of pH value, breast meat colour, WHC, drip loss, the relative organ weights of liver, spleen, bursa of Fabricius and breast muscle were not affected by any dietary supplementation (P &gt; 0.05). The relative weight of abdominal fat in the CON treatment group was significantly higher than the YGF0.05 and YGF0.1 treatments, although relative gizzard weight was lower with CON treatment compared to YGF0.1 treatment (P &lt; 0.05). Femur length and weight were significantly higher in the YGF251-supplemented chicks than in chicks fed the control diet (P &lt; 0.05). Broiler chickens fed the YGF0.1 diet had significantly higher blood IgG counts compared to chicks fed the CON and YGF0.05 diets (P &lt; 0.05). Moreover, YGF251-supplemented chicks exhibited increased IGF-I concentrations compared to the CON to YGF0.1 treatment (P &lt; 0.05). The results of this study indicate that supplementation with 0.1% YGF251 can increase body weight, IgG and energy digestibility and reduce relative abdominal fat and gizzard weight in broiler chickens, while at all concentrations tested YGF251-supplemented chicks showed higher results for femur length and weight and serum IGF-I concentrations compared to the control treatment in broiler chickens. &nbsp;</P>

Identification of Breeding Resource Material for the Development of Therms-Tolerant Breeds in the silkworm, Bombyx mori

Begum, A.Naseema,Basavaraja, H.K.,Rekha, M.,Ahsan, M.M.,Datta, R.K. Korean Society of Sericultural Science 2001 International Journal of Industrial Entomology Vol.2 No.2

Screening of fifteen bivoltine silkworm breeds of Bombyx mori Linn at a temperature of $31{\pm}1^{\circ}C$ and relative humidity of 85${\pm}$5% resulted in the identification of eight thermo-tolerant breeds. The survival and cocoon shell ratio of the tolerant breeds ranged from 72.7 to 78.7% and 20.0 to 20.1% respectively. The tolerant breeds comprised of four oval breeds and four dumb-bell breeds. Eight foundation crosses prepared by crossing the oval and dumb-bell parents among themselves were screened at a temperature of $31{\pm}1^{\circ}C$ and relative humidity of 85{\pm}5%. The performance of the foundation crosses on 11 economic characters were analysed by employing Multipie Trait Evaluation Index method. Four foundation crosses which scored average index value > 50 were selected as breeding parents and breeding initiated for the evolution of thermo-tolerant bivoltine silkworm breeds. The methodology and the results of the foundation crosses reared both at 31{\pm}1$^{\circ}C$ and at $25{\pm}1^{\circ}C$ temperatures, are discussed.

Synergistic effects of urban tributary mixing on dissolved organic matter biodegradation in an impounded river system

Begum, Most Shirina,Jang, Inae,Lee, Jung-Min,Oh, Han Bin,Jin, Hyojin,Park, Ji-Hyung Elsevier 2019 The Science of the total environment Vol.676 No.-

<P><B>Abstract</B></P> <P>Dams and wastewater may greatly perturb riverine fluxes of dissolved organic matter (DOM) and CO<SUB>2</SUB>, yet little is known about the relationships between altered DOM quality and CO<SUB>2</SUB> emission in eutrophic impounded river systems. A basin-wide field survey of surface water CO<SUB>2</SUB> and dissolved organic carbon (DOC) was combined with laboratory incubations to examine how dams and urban tributaries delivering treated wastewater influence longitudinal patterns in DOM properties and CO<SUB>2</SUB> along the impounded Han River traversing Seoul metropolitan area. Fluorescent DOM indices including parallel factor analysis (PARAFAC) components were used to characterize DOM in relation to biodegradable DOC (BDOC). Compared with distinct downstream increases in DOC and CO<SUB>2</SUB>, BDOC concentration and its proportion in DOC (%BDOC) were highly variable along the mainstem and peaked at urban tributaries. Longitudinal increases in fluorescence index (FI), biological index (BIX), and two PARAFAC components (C2 and C3) contrasted with general decreases in humification index (HIX) and C1, reflecting increasing downstream inputs of anthropogenic DOM. During a 5-day incubation employing continuous CO<SUB>2</SUB> measurements, the cumulative production of CO<SUB>2</SUB> in the mainstem water mixed with urban tributary water was significantly higher than the level expected for conservative mixing of the two samples, indicating a synergistic enhancement of DOM biodegradation. Molecular formulas identified by Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS) revealed more consumed molecules in the mainstem water and more newly produced molecules in the tributary water over the 5-day incubation, implying abundant labile components in the mainstem water discharged from the upstream dam and highly processed tributary DOM limited in immediately biodegradable organic materials. Downstream increases in CO<SUB>2</SUB> and DOC along the Han River, combined with the synergistic effect observed in the mixed water, suggest that mixing wastewater-derived DOM with labile autochthonous DOM can enhance CO<SUB>2</SUB> production in the river system perturbed by impoundment and wastewater.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Field survey was combined with lab incubations to study human impact on river carbon. </LI> <LI> Dissolved organic matter and CO<SUB>2</SUB> vary along impounded river with wastewater influence. </LI> <LI> Urban tributary mixing enhances organic matter degradation and CO<SUB>2</SUB> in impounded river. </LI> <LI> FT-ICR-MS detects organic matter degradation in impounded river and urban tributary. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

CA 15-3 (Mucin-1) and Physiological Characteristics of Breast Cancer from Lahore, Pakistan

Begum, Mumtaz,Karim, Sajjad,Malik, Arif,Khurshid, Rukhshan,Asif, Muhammad,Salim, Asmat,Nagra, Saeed Ahmed,Zaheer, Ahmad,Iqbal, Zafar,Abuzenadah, Adel Mohammed,Alqahtani, Mohammed Hussain,Rasool, Mahmo Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Background: High incidence of breast cancer and its fatal effect has reached an alarming stage across the globe, including the third world countries. Many factors have been reported to be associated with the development of breast cancer but detailed structural and functional information is missing. CA 15-3 is one of the known potential tumor marker of breast cancer; however little is known about structure and functional site of this protein. Present study aims to investigate the functional role of CA 15-3 in breast cancer, especially in development and metastasis. Material and Methods: Hundred female breast cancer patients confirmed by histopathological reports were included in the study. Their physiological characters were recorded in a performa. Enzyme linked immunosorbent assay (ELISA) technique was used to estimate serum CA 15-3 level. Immunohistochemistry was done for estrogen (ER), progesterone (PR) and Her2/neu receptors expression. Results: The study revealed the details of physiological characteristics of female breast cancer. Mean age was $37.72{\pm}5.99$ and $55.05{\pm}7.28$ years and serum CA 15-3 (MUC1) level was $60.47{\pm}8.59$ and $63.17{\pm}4.58$ U/ml in pre and post-menopause respectively, and both groups of women had sedentary life style. Their receptor status especially of progesterone, estrogen and HER-2/neu were positive in 50% of premenopausal women and 65% of postmenopausal women. Conclusion: There are multiple physiological factors promoting breast cancer. High serum CA 15-3 level and hormonal imbalance of ER, PR and Her2/neu appears to be the main cause of breast cancer. It may be possible that the functional sites of these proteins may be altered which may increase the chances of metastasis in breast cancer.

Initiating Smokeless Tobacco Use across Reproductive Stages

Begum, Shahina,Schensul, Jean J.,Nair, Saritha,Donta, Balaiah Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Background: The use of smokeless tobacco (SLT) among women is increasing in India, especially among those with limited education and resources. Preventing the initiation of SLT among women is critical since it has known negative consequences for oral and reproductive health. Most research on tobacco initiation in India focuses on adolescents. This paper addresses the unrecognized issues of post marital initiation among women of reproductive age, highlighting the importance of reproductive stages in women's tobacco initiation. The objective is to examine the correlates of SLT initiation among low income women in Mumbai from pre-marriage through early marriage, first pregnancy and beyond, using case examples to illustrate initiation during each of these stages. Materials and Methods: In 2011-2012, cross-sectional community level survey data were collected from a representative sample of 409 daily SLT-using married women aged 18-40 years in a low income community in Mumbai. Information on socio-demographics, initiation by reproductive stage, types of tobacco use, childhood exposure to tobacco, learning to use, and initiation influences and reasons were collected through a researcher-administered survey. Univariate and bivariate analysis assessed factors influencing initiation of SLT use by reproductive stage. In addition 42 narratives of tobacco use were collected from a purposive sample of pregnant and non-pregnant married women addressing the same questions in detail. Narratives were transcribed, translated, and coded for key concepts including initiation of tobacco use. Results: Thirty-two percent of women initiated SLT use before marriage, 44% initiated after marriage but before pregnancy, 18.1% initiated during their first pregnancy and the remainder started after their first pregnancy. Mean age of marriage among women in this study was 16 years. Younger women (i.e. age at time of the interview of less than 30 years) were 0.47 [95% CI (0.32, 0.87)] percent less likely to initiate after marriage than women aged more than 30 years. Women who got married before 18 years of age were 2.34 [95% CI (1.40, 3.93)] times more likely to initiate after marriage than their counterparts. Childhood exposure was a predictor for initiating SLT use prior to marriage but not after. Women reporting tooth and gum pain were 1.85 times more likely to initiate after marriage than their counterparts. Husband and neighbours were the most significant influences on post-marital initiation. Narratives highlighted differences in processes of initiation pre and post marriage and during pregnancy. Conclusions: Most tobacco prevention interventions are directed to adolescents in school. This study suggests that especially for low literate or illiterate women, school based interventions are ineffective. To be effective strategies to prevent SLT initiation must reach women in urban areas at or immediately after marriage and during their first pregnancy. Messages must negate culturally rooted beliefs about the health benefits of SLT in order to prevent initiation and onset of daily use.

Effect of Aerva lanata against oxalate mediated free radical toxicity in urolithiasis

Begum, Vava Mohaideen Hazeena,Mahesh, Ramalingam,Ramesh, Thiyagarajan,Soundararajan, Periasamy Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.1

This study was undertaken to evaluate the antioxidant potential of A. lanata on oxalate mediated free radical toxicity in ethylene glycol induced calcium oxalate urolithic rats. Calcium oxalate (CaOX) stone was induced by 0.75% ethylene glycol in drinking water for 28 days. From $29^{th}$ day onwards, the CaOX urolithic rats were treated with A. lanata aqueous suspension (2,000 mg/kg body weight/dose/day) orally for another 28 days. At the end of experimental periods the animals were sacrificed, samples were collected and analyzed the lipid peroxidation product, protein oxidation product, enzymatic and non-enzymatic antioxidants in normal and experimental groups. Lipid peroxidation and protein oxidation products were significantly elevated while enzymatic and non-enzymatic antioxidant levels were significantly decreased in ethylene glycol induced CaOX urolithic rats when compared with control rats. The above alterations were reverted to near control in rats treated with aqueous suspension of A. lanata. This study suggests that A. lanata could prevent the free radical formation from calcium oxalate urolithiasis in rats and protecting the renal cells from oxidative injury.

Secondary metabolites (Triterpenes) from Couroupita guianensis

Begum, Rokeya,Rahman, Mohammad S,Chowdhury, A M Sarwaruddin,Hasan, Choudhury M,Rashid, Mohammad A Kyung Hee Oriental Medicine Research Center 2009 Oriental pharmacy and experimental medicine Vol.9 No.2

The n-hexane and carbon tetrachloride soluble fractions of a methanolic extract of the stem bark of the Couroupita guianensis furnished three compounds, identified as $\beta$-amyrin (1), betulin-$3{\beta}$-caffeate (2) and lupeol-$3{\beta}$-caffeate (3). The structures of the isolated compounds were deduced by extensive spectroscopic analysis as well as by comparison with published values. Compounds 1-3 were subjected to antioxidant screening through free radical scavenging activity by DPPH (1,1-diphenyl-2-picrylhydrazyl), where compound 2 showed moderate antioxidant activity with $IC_{50}$ value $108.0{\mu}g/ml$.