A Rare Stinkhorn Fungus Itajahya rosea Attract Drosophila by Producing Chemical Attractants

( Mahesh Borde ),( Yogesh Kshirsagar ),( Reshma Jadhav ),( Abhishek Baghela ) 한국균학회 2021 Mycobiology Vol.49 No.3

Itajahya rosea was found growing in association with Leucaena leucocephala plants at Savitribai Phule Pune University campus in India. The species identity was confirmed by phylogenetic analysis based on ITS and LSU regions of rDNA, wherein, our fugus was placed along with I. rosea in phylogenetic tree. It represents first record of I. rosea from India. Frequent visitation by Drosophila species on I. rosea fruiting body particularly on gleba was observed. The Drosophila got attracted to the detached gleba under the laboratory conditions and even sometimes, they prefer to sit over the gleba as compare to their food banana. It suggested that I. rosea gleba or pseudostipe produces some compounds for attraction and feeding behavior of Drosophila species. Therefore, we characterized the volatile attractants produced by gleba and pseudostipe of I. rosea by GC-MS analysis. Nineteen compounds were identified from gleba while nine compounds were recovered from the pseudostipe. Out of them, blends of three abundant odor producing volatile compounds were reported namely, Hexadecane, Pentadecane and Nonadecane, which are responsible for attraction of Drosophila toward the gleba. Three fatty acids namely 9,12-octadecadienoic acid (Z,Z), hexadecanoic acid and benzoic acid ethyl ester produced are served as an appetitive signal through olfactory response of Drosophila, so the flies were feed on the gleba. Two pheromones’ compounds, heneicosane and (þ)-(5S,9S)-5,9-dimethylpentadecane, were also reported in pseudostipe and gleba, respectively, which play a role in Drosophila for breeding. Our study highlights an intriguing chemical ecology of fungus-Drosophila interaction.

Fungal Production of Single Cell Oil Using Untreated Copra Cake and Evaluation of Its Fuel Properties for Biodiesel

( Mahesh Khot ),( Rohini Gupta ),( Kadambari Barve ),( Smita Zinjarde ),( Sanjay Govindwar ),( Ameeta Ravikumar ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.4

This study evaluated the microbial conversion of coconut oil waste, a major agro-residue in tropical countries, into single cell oil (SCO) feedstock for biodiesel production. Copra cake was used as a low-cost renewable substrate without any prior chemical or enzymatic pretreatment for submerged growth of an oleaginous tropical mangrove fungus, Aspergillus terreus IBB M1. The SCO extracted from fermented biomass was converted into fatty acid methyl esters (FAMEs) by transesterification and evaluated on the basis of fatty acid profiles and key fuel properties for biodiesel. The fungus produced a biomass (8.2 g/l) yielding 257 mg/g copra cake SCO with ~98% FAMEs. The FAMEs were mainly composed of saturated methyl esters (61.2%) of medium-chain fatty acids (C12-C18) with methyl oleate (C18:1; 16.57%) and methyl linoleate (C18:2; 19.97%) making up the unsaturated content. A higher content of both saturated FAMEs and methyl oleate along with the absence of polyunsaturated FAMEs with ≥4 double bonds is expected to impart good fuel quality. This was evident from the predicted and experimentally determined key fuel properties of FAMEs (density, kinematic viscosity, iodine value, acid number, cetane number), which were in accordance with the international (ASTM D6751, EN 14214) and national (IS 15607) biodiesel standards, suggesting their suitability as a biodiesel fuel. The low cost, renewable nature, and easy availability of copra cake, its conversion into SCO without any thermochemical pretreatment, and pelleted fungal growth facilitating easier downstream processing by simple filtration make this process cost effective and environmentally favorable.

Three New Records of Ascomycetes Isolates from Field Soils in Korea

( Mahesh Adhikari ),( Sun Kumar Gurung ),( Hyun Seung Kim ),( Setu Bazie ),( Hyun Gu Lee ),( Hyang Burm Lee ),( Youn Su Lee ) 한국균학회 2017 Mycobiology Vol.45 No.4

Three new records of Ascomycota species (Chaetomium acropullum, Phialemonium globosum, Phialemonium atrogriseum) from field soils in Korea are presented in this study. These newly discovered fungal isolates were isolated from field soils from various places across Gyeongnam, Korea in 2016. All the isolates were identified and described based on morphological characteristics, and rDNA internal transcribed spacer and β-tubulin gene sequence data. Morphological features of these fungal species were studied on different agar media: potato dextrose agar, oatmeal agar, malt extract agar, Czapek yeast extract agar, and yeast extract sucrose agar. Full description and illustrations of their morphological characters are provided. These fungal species have not officially been previously reported in Korea.

Mucosal Changes in the Small Intestines in Portal Hypertension: First Study Using the Pillcam SB3 Capsule Endoscopy System

Mahesh Kumar Goenka,Bhavik Bharat Shah,Vijay Kumar Rai,Surabhi Jajodia,Usha Goenka 대한소화기내시경학회 2018 Clinical Endoscopy Vol.51 No.6

Background/Aims: To evaluate patients with portal hypertension (PH) of varied etiologies for portal hypertensive enteropathy (PHE)using the PillCam SB3 capsule endoscopy (CE) system. Methods: Consecutive patients with PH presenting with unexplained anemia and/or occult gastrointestinal bleeding were evaluatedusing the PillCam SB3 CE system. Abnormal findings were categorized as vascular or non-vascular. The patients with ongoing bleedingcaused by PHE were treated. The correlation of the CE scores of PHE with the clinical, laboratory, and endoscopic features wasdetermined. Results: Of the 43 patients included in the study, 41 (95.3%) showed PHE findings. These included varices (67.4%), red spots (60.5%),erythema (44.2%), villous edema (46.5%), telangiectasia (16.3%), and polyps (16.3%). The CE scores varied from 0 to 8 (mean±standarddeviation, 4.09±1.8). Five patients (11.6%) showed evidence of ongoing or recent bleeding due to PHE. Three of these five patientsunderwent endotherapy, and one patient underwent radiological coil placement. Conclusions: The PillCam SB3 CE system revealed a high prevalence of PHE in the patients with PH. Using this system, evidence ofbleeding due to PHE was found in a small but definite proportion of the patients.

Seven Unrecorded Fungal Species from Field Soils in Korea

( Mahesh Adhikari ),( Sun Kumar Gurung ),( Setu Bazie ),( Hyun Gu Lee ),( San Kosol ),( Hyang Burm Lee ),( Youn Su Lee ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.1

Seven unrecorded species in the phylum Ascomycota, Emericellopsis pallida (KNU16-167), Scedosporium aurantiacum (KNU16-190), Duddingtonia flagrans (KNU16-279), Bionectria rossmaniae (KNU16-309), Exophiala xenobiotica (KNU16-79), Pseudocercosporella fraxini (KNU16-102), and Stachybotrys sansevieriae (KNU16-141), were isolated in 2016 from field soils collected from various locations in Korea. All of the species were identified and described based on morphological characteristics and rDNA internal transcribed spacer sequence data. Morphological features of these fungi were examined on potato dextrose agar, oatmeal agar, malt extract agar, Czapek yeast extract agar, and yeast extract sucrose agar. Full descriptions and illustrations of their morphological characteristics are provided.

Private Police in Emerging Markets: South Korea

Mahesh K,Nalla,Eui-Gab Hwang 아시아경찰학회 2003 아시아경찰학회 국제학술회의 Vol.2003 No.-

The Republic of Korea (South Korea) has made significant progress in the market sector in the past four decades. Relative to its neighbors in Asia, South Korea has increased its market base both in terms of production and consumption. Along with economic growth, South Korea has also experienced an increase in the employment of private security personnel. Despite large increases in the growth of the private policing industry in the last four decades in the emerging markets, we know very little about how the public perceives agents of private policing. In this paper we examine South Korean youth perceptions of private security officers. Responses were received from 172 college students. The findings suggest that respondents generally held positive attitudes toward security work and security officers.

Alternative Methods for Testing Botulinum Toxin: Current Status and Future Perspectives

( Mahesh Raj Nepal ),( Tae Cheon Jeong ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.4

Botulinum toxins are neurotoxic modular proteins composed of a heavy chain and a light chain connected by a disulfide bond and are produced by Clostridium botulinum. Although lethally toxic, botulinum toxin in low doses is clinically effective in numerous medical conditions, including muscle spasticity, strabismus, hyperactive urinary bladder, excessive sweating, and migraine. Globally, several companies are now producing products containing botulinum toxin for medical and cosmetic purposes, including the reduction of facial wrinkles. To test the efficacy and toxicity of botulinum toxin, animal tests have been solely and widely used, resulting in the inevitable sacrifice of hundreds of animals. Hence, alternative methods are urgently required to replace animals in botulinum toxin testing. Here, the various alternative methods developed to test the toxicity and efficacy of botulinum toxins have been briefly reviewed and future perspectives have been detailed.

Flip-OFDM Optical MIMO Based VLC System Using ML/DL Approach

Mahesh Kumar Jha,Rubini P,Navin Kumar 한국정보과학회 2023 Journal of Computing Science and Engineering Vol.17 No.2

Endoscopic Management with a Novel Over-The-Scope Padlock Clip System

Mahesh Kumar Goenka,Gajanan Ashokrao Rodge,Indrajeet Kumar Tiwary 대한소화기내시경학회 2019 Clinical Endoscopy Vol.52 No.6

Background/Aims: The Padlock clip is a recently introduced over-the-scope clip (OTSC) that requires the use of an alternate techniqueand has a different design from previous OTSCs. However, data regarding its clinical use are limited. The aim of this study is to presentour clinical experience using this novel Padlock clip system. Methods: Between September 2018 and June 2019, 7 consecutive patients underwent Padlock clip application at our center by anexperienced endoscopist. A Padlock clip was used for achieving hemostasis in 4 patients presenting with gastrointestinal (GI) bleeding,as well as for endoscopic full-thickness resection in the remaining 3 patients. Results: All 7 patients achieved technical as well as clinical success, with absence of complications or rebleeding, during a follow-up ofa minimum of 3 weeks. All patients were hospitalized post procedure for a minimum of 48 hours, and an absence of adverse events wasnoted in our patient population throughout the procedure and post-procedure period. Antiplatelet therapy was reinstated shortly afterthe application of the Padlock clip, with no GI bleeding observed. Conclusions: The Padlock clip is a novel OTSC, with benefits that include safe, simple, and rapid deployment. Antiplatelet therapy maybe reinstated for patients, when necessary, shortly after applying the Padlock clip due to full-thickness closure of the tissue.

Pancreatic Necrosectomy through Sinus Tract Endoscopy

Mahesh Kumar Goenka,Usha Goenka,Md.Yasin Mujoo,Indrajit Kumar Tiwary,Sanjay Mahawar,Vijay Kumar Rai 대한소화기내시경학회 2018 Clinical Endoscopy Vol.51 No.3

Background/Aims: Direct endoscopic pancreatic necrosectomy is increasingly being utilized to treat infected or symptomatic walledoffnecrosis (WON) located close to the stomach or duodenum. Laterally-placed WON has traditionally been treated surgically. Weevaluated a less utilized technique of sinus tract endoscopy (STE) for symptomatic laterally-placed WON. Methods: Two hundred seventy-six patients with acute pancreatitis admitted in our hospital, 32 had symptomatic or infected WONrequiring intervention. Of the 12 patients with laterally placed WON, 10 were treated by STE. STE was performed with a standard adultgastroscope passed through a percutaneous tract created by the placement of a 32-Fr drain. Results: Ten patients (7 males; mean age, 43.8 years) underwent STE. Mean number of sessions was 2.3 (range, 1–4), with mean timeof 70 minutes for each session (range, 15–70 minutes). While 9 patients had complete success, 1 patient had fever and chose to undergosurgery. Two patients developed pneumoperitoneum, which was treated conservatively. There was no mortality, cutaneous fistula, orrecurrence during follow-up. Conclusions: Laterally placed WON can be successfully managed by STE performed through a percutaneously placed drain. Details ofthe technique and end-points of STE require further evaluation.