Effects of Periparturient Anthelmintic Treatment on the Milk Yield in Cows

Md. Faruk Islam,Begum Fatema Zohara,Nurjahan Begum,Md. Golam Shahi Alam 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.2

This study was conducted to determine the efficacy of anthelmintics against gastrointestinal parasitic infestation inperiparturient dairy cows and its effect on milk yield and quality. Sixty pregnant cows of 1st & 2nd parity weredivided into four groups. The efficacy of anthelmintic treatment was evaluated by counting faecal egg per gram (EPG)compared with pre-treatment values. The milk yield of each cow was recorded in pre and post treatment lactations. Cows of group A were treated with Nitroxynil 10 mg/kg body weight subcutaneously 30 days before parturition, groupB were treated with combination of triclabendazole and levamisole 19.5 mg/kg body weight orally at calving andgroup C treated with Endex® at calving and 42 days after. The mean change in EPG 14 days after treatment wassignificantly higher (p<0.05) in treated (79.1%) cows than control. Average milk yield of group C (2.8 ± 0.8) wassignificantly higher (p<0.01) than group A (2.6 ± 0.7). Similarly, the average milk yield in all the treated cows wassignificantly (p<0.01) higher in treated lactation (2.5 ± 0.7) than in the previous lactation (2.2 ± 0.7). The averagemilk yield in all treated cows was significantly (p<0.01) higher than the control cows. Although, milk yields werehigher in second parity than the first, there was no significant difference (p>0.05). The milk protein percentage wassignificantly higher (p<0.05) in treated group than the control group. Therefore, it may be concluded that periparturientanthelmintic treatment effectively reduced the gastrointestinal parasitic load and improved milk yield.

Effects of Periparturient Anthelmintic Treatment on the Milk Yield in Cows

Islam, Faruk Md.,Zohara, Begum Fatema,Begum, Nurjahan,Alam, Md. Golam Shahi 韓國受精卵移植學會 2014 한국동물생명공학회지 Vol.29 No.2

This study was conducted to determine the efficacy of anthelmintics against gastrointestinal parasitic infestation in periparturient dairy cows and its effect on milk yield and quality. Sixty pregnant cows of 1st & 2nd parity were divided into four groups. The efficacy of anthelmintic treatment was evaluated by counting faecal egg per gram (EPG) compared with pre-treatment values. The milk yield of each cow was recorded in pre and post treatment lactations. Cows of group A were treated with Nitroxynil 10 mg/kg body weight subcutaneously 30 days before parturition, group B were treated with combination of triclabendazole and levamisole 19.5 mg/kg body weight orally at calving and group C treated with Endex® at calving and 42 days after. The mean change in EPG 14 days after treatment was significantly higher (p<0.05) in treated (79.1%) cows than control. Average milk yield of group C (2.8 ± 0.8) was significantly higher (p<0.01) than group A (2.6 ± 0.7). Similarly, the average milk yield in all the treated cows was significantly (p<0.01) higher in treated lactation (2.5 ± 0.7) than in the previous lactation (2.2 ± 0.7). The average milk yield in all treated cows was significantly (p<0.01) higher than the control cows. Although, milk yields were higher in second parity than the first, there was no significant difference (p>0.05). The milk protein percentage was significantly higher (p<0.05) in treated group than the control group. Therefore, it may be concluded that periparturient anthelmintic treatment effectively reduced the gastrointestinal parasitic load and improved milk yield.

Exfoliative Vaginal Cytology and Serum Progesterone during the Estrous Cycle of Indigenous Ewes in Bangladesh

Begum Fatema Zohara,Azizunnesa,Md. Faruk Islam,Md. Golam Shahi Alam,Farida Yeasmin Bari 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.2

A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, thepattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cyclelength and duration of estrus were 15.8±0.12 days and 31.1±0.57 h respectively. The exfoliated epithelial cells werecategorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages ofparabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell typeduring estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated byneutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to 1.6±0.07 ng/ml. The length ofdiestrus was 5∼10 days with a range of mean progesterone level of 1.6±0.07 to 2.8±0.11 ng/ml. Progesterone levelsincreased significantly (p<0.01) after Day 5 and maximum level was 2.8±0.11 ng/ml observed on Day 10 of theestrous cycle. Thereafter it dropped rapidly to basal level of 0.11±0.04 ng/ml on Day 0 (p<0.01). These results indicatethat the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine thereproductive stages of indigenous ewe

Effects of number of frozen-thawed ram sperm and number of inseminations on fertility in synchronized ewes under field condition

Pankaj Kumar Jha,Md. Golam Shahi Alam,Md. Abdullah Al Mansur,Mohammad Rafiqul Islam Talukder,Nazmun Naher,A. K. M. Anisur Rahman,David C. Hall,Farida Yeasmin Bari 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.2

The effects of the number of frozen-thawed ram sperm per single and double intra-cervical artificial insemination (AI) on fertility in ewes were studied. A total of 89 non-pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; D200 (n = 23; double AI with 200 × 106 sperm), S200 (n = 24; single AI with 200 × 106 sperm), D100 (n = 24; double AI with 100 × 106 sperm) and S100 (n = 18; single AI with 100 × 106 sperm). Ewes were inseminated within 12 to 18 h for single AI and, within 10 to 12 h and 16 to 18 h for double AI after the onset of oestrus. The onset of oestrus ranged from 28 to 76 h (54.33 ± 1.28 h). The high percentage (29.2%) of ewes showed oestrus between 51 to 60 h. The non-return rates were highest in group D200 (56.5%) and differed significantly (p < 0.05) from group S100 (11.1%). No ewes were pregnant in group S100, and the pregnancy rates among the remaining groups did not differ. The mean gestation period was 152.8 ± 0.5 days and no difference was observed among the groups. The lambing and multiple birth rates were 100% in group D200. The single and twin lambing was highest in group D100 (33.3%) and group D200 (83.3%), respectively. Only one triplet lambing and the highest lambing size (2.2 ± 0.2) was recorded in group D200. In conclusion, double AI with 200 × 106 sperm showed comparatively most practical for achieving high pregnancy rates and lambing performances in Bangladeshi ewes under field conditions.

Effects of number of frozen-thawed ram sperm and number of inseminations on fertility in synchronized ewes under field condition

Pankaj Kumar Jha,Md. Golam Shahi Alam,Md. Abdullah Al Mansur,Mohammad Rafiqul Islam Talukder,Nazmun Naher,A K M Anisur Rahman,David C. Hall,Farida Yeasmin Bari 사단법인 한국동물생명공학회 2020 한국동물생명공학회지 Vol.35 No.2

The effects of the number of frozen-thawed ram sperm per single and double intra-cervical artificial insemination (AI) on fertility in ewes were studied. A total of 89 non-pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; D200 (n = 23; double AI with 200 × 106 sperm), S200 (n = 24; single AI with 200 × 106 sperm), D100 (n = 24; double AI with 100 × 106 sperm) and S100 (n = 18; single AI with 100 × 106 sperm). Ewes were inseminated within 12 to 18 h for single AI and, within 10 to 12 h and 16 to 18 h for double AI after the onset of oestrus. The onset of oestrus ranged from 28 to 76 h (54.33 ± 1.28 h). The high percentage (29.2%) of ewes showed oestrus between 51 to 60 h. The non-return rates were highest in group D200 (56.5%) and differed significantly (p < 0.05) from group S100 (11.1%). No ewes were pregnant in group S100, and the pregnancy rates among the remaining groups did not differ. The mean gestation period was 152.8 ± 0.5 days and no difference was observed among the groups. The lambing and multiple birth rates were 100% in group D200. The single and twin lambing was highest in group D100 (33.3%) and group D200 (83.3%), respectively. Only one triplet lambing and the highest lambing size (2.2 ± 0.2) was recorded in group D200. In conclusion, double AI with 200 × 106 sperm showed comparatively most practical for achieving high pregnancy rates and lambing performances in Bangladeshi ewes under field conditions.

Relationship between Semen Quality Parameters and Field Fertility of Bulls

Sushanto Kumar Rabidas,Anup Kumar Talukder,Md. Golam Shahi Alam,Farida Yeasmin Bari 사단법인 한국동물생명공학회 2012 한국동물생명공학회지 Vol.27 No.1

A study was conducted on four crossbred bulls, used as artificial insemination (AI) sires, to correlate their semen quality with their non return rate (NRR). Semen was collected once a week via an artificial vagina, diluted in egg yolk-citrate and maintained at +7℃ for three days. It was evaluated for sperm motility, viability, morphology immediately after collection and was examined daily for sperm motility, viability and morphology of acrosome, mid piece and tail for a total of three days. A total of 2016 cows were inseminated by two AI technicians. The proportions of sperm with normal heads were 83.4% (63.7~91.7%), the proportion of spermatozoa exhibiting normal morphology (acrosome, mid piece and tail), motility and viability were 89.2% (82.3~92.0%), 71.3% (61.7~75.0%) and 76.7% (65.7~85.0%), respectively in fresh ejaculates. Sperm motility and sperm viability was significantly (p<0.05) lower in Holstein-Friesian × Local bull than in other bulls during all three days of storage. The overall NRR for four bulls was 82.7% (72.9-87.5%). Bulls with higher sperm motility, viability and normal morphology of spermatozoa of individual bull had significantly (each p<0.05) higher NRR. The highest (p<0.01) NRR (87.5%) was observed in a Red Chittagong bull whose semen qualities were significantly (p<0.05) higher than Holstein-Friesian × Local bull (NNR 72.9%). The results of the present study concluded that NRR at 56 days post AI is related to parameters of semen quality. Therefore, semen evaluation may allow the discarding of bulls with poor fertility in an AI program

Exfoliative Vaginal Cytology and Serum Progesterone during the Estrous Cycle of Indigenous Ewes in Bangladesh

Zohara, Begum Fatema,Azizunnesa, Azizunnesa,Islam, Md. Faruk,Alam, Md. Golam Shahi,Bari, Farida Yeasmin 韓國受精卵移植學會 2014 한국동물생명공학회지 Vol.29 No.2

A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, the pattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cycle length and duration of estrus were 15.8±0.12 days and 31.1±0.57 h respectively. The exfoliated epithelial cells were categorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages of parabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell type during estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated by neutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to 1.6±0.07 ng/ml. The length of diestrus was 5∼10 days with a range of mean progesterone level of 1.6±0.07 to 2.8±0.11 ng/ml. Progesterone levels increased significantly (p<0.01) after Day 5 and maximum level was 2.8±0.11 ng/ml observed on Day 10 of the estrous cycle. Thereafter it dropped rapidly to basal level of 0.11±0.04 ng/ml on Day 0 (p<0.01). These results indicate that the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine the reproductive stages of indigenous ewe.

Relationship between Semen Quality Parameters and Field Fertility of Bulls

Rabidas, Sushanto Kumar,Talukder, Anup Kumar,Alam, Md. Golam Shahi,Bari, Farida Yeasmin 韓國受精卵移植學會 2012 한국동물생명공학회지 Vol.27 No.1

A study was conducted on four crossbred bulls, used as artificial insemination (AI) sires, to correlate their semen quality with their non return rate (NRR). Semen was collected once a week via an artificial vagina, diluted in egg yolk-citrate and maintained at for three days. It was evaluated for sperm motility, viability, morphology immediately after collection and was examined daily for sperm motility, viability and morphology of acrosome, mid piece and tail for a total of three days. A total of 2016 cows were inseminated by two AI technicians. The proportions of sperm with normal heads were 83.4% (63.7~91.7%), the proportion of spermatozoa exhibiting normal morphology (acrosome, mid piece and tail), motility and viability were 89.2% (82.3~92.0%), 71.3% (61.7~75.0%) and 76.7% (65.7~85.0%), respectively in fresh ejaculates. Sperm motility and sperm viability was significantly ( <0.05) lower in Holstein-Friesian Local bull than in other bulls during all three days of storage. The overall NRR for four bulls was 82.7% (72.9-87.5%). Bulls with higher sperm motility, viability and normal morphology of spermatozoa of individual bull had significantly (each <0.05) higher NRR. The highest ( <0.01) NRR (87.5%) was observed in a Red Chittagong bull whose semen qualities were significantly ( <0.05) higher than Holstein-Friesian Local bull (NNR 72.9%). The results of the present study concluded that NRR at 56 days post AI is related to parameters of semen quality. Therefore, semen evaluation may allow the discarding of bulls with poor fertility in an AI program.

Effects of Concentrate Supplementation on Reproductive Performances and Semen Quality of Indigenous Rams in Bangladesh

Azizunnesa, Azizunnesa,Zohara, Begum Fatema,Bari, Farida Yeasmin,Alam, Md. Golam Shahi The Korean Society of Embryo Transfer 2013 한국동물생명공학회지 Vol.28 No.4

The study was set for one year to measure the effects of concentrate supplementation on reproductive performances and semen quality in indigenous rams. The study was conducted at the Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh during the period from May 2011 to April 2012. Forteen ram lambs (4~5 months) were randomly divided into two equal groups (n=7); supplemented vs control. The animals of control group were maintained on natural grazing. Along with natural grazing the supplemented group was on supplemented feeding. The concentrate supplementation (Wheat bran, Crushed maize, Soy bean meal, Fish meal, DCP powder, Vitamin mineral premix, Salt) was provided @ 300 g/head /day to the supplemented group. Body weight, scrotal circumference, BCS and libido index were measured weekly. Age, body weight and scrotal circumference at puberty were recorded. Semen was collected once in a weak using artificial vagina and chilled at $5^{\circ}C$ for 48h for evaluation. Concentrate supplementation did not influence (p>0.05) body condition score, age, weight, scrotal circumference at puberty and libido index. Final body weight (kg), growth rate (g/d), scrotal circumference (cm) and scrotal growth rate (mm/15d) were significantly (p<0.05) higher in supplemented group of rams compared to control. Volume, concentration, motility and membrane potentiality of spermatozoa were varied significantly (p<0.05) in supplemented and control groups. However, density, mass motility, viability and sperm with normal acrosome, midpiece and tail were not differed insignificantly (p>0.05) in different observation times. It was concluded that concentrate supplementation with free grazing improved weight and scrotal circumference gain and semen production with increased quality in indigenous ram.