Effects of Concentrate Supplementation on Reproductive Performances and Semen Quality of Indigenous Rams in Bangladesh

Azizunnesa,Begum Fatema Zohara,Farida Yeasmin Bari,Golam Shahi Alam 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.4

The study was set for one year to measure the effects of concentrate supplementation on reproductive performances and semen quality in indigenous rams. The study was conducted at the Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh during the period from May 2011 to April 2012. Forteen ram lambs (4∼5 months) were randomly divided into two equal groups (n=7); supplemented vs control. The animals of control group were maintained on natural grazing. Along with natural grazing the supplemented group was on supplemented feeding. The concentrate supplementation (Wheat bran, Crushed maize, Soy bean meal, Fish meal, DCP powder, Vitamin mineral premix, Salt) was provided @ 300 g/head /day to the supplemented group. Body weight, scrotal circumference, BCS and libido index were measured weekly. Age, body weight and scrotal circumference at puberty were recorded. Semen was collected once in a weak using artificial vagina and chilled at 5℃ for 48h for evaluation. Concentrate supplementation did not influence (p>0.05) body condition score, age, weight, scrotal circumference at puberty and libido index. Final body weight (kg), growth rate (g/d), scrotal circumference (cm) and scrotal growth rate (mm/15d) were significantly (p<0.05) higher in supplemented group of rams compared to control. Volume, concentration, motility and membrane potentiality of spermatozoa were varied significantly (p<0.05) in supplemented and control groups. However, density, mass motility, viability and sperm with normal acrosome, midpiece and tail were not differed insignificantly (p>0.05) in different observation times. It was concluded that concentrate supplementation with free grazing improved weight and scrotal circumference gain and semen production with increased quality in indigenous ram.

Effects of Concentrate Supplementation on Reproductive Performances and Semen Quality of Indigenous Rams in Bangladesh

Azizunnesa, Azizunnesa,Zohara, Begum Fatema,Bari, Farida Yeasmin,Alam, Md. Golam Shahi The Korean Society of Embryo Transfer 2013 한국동물생명공학회지 Vol.28 No.4

The study was set for one year to measure the effects of concentrate supplementation on reproductive performances and semen quality in indigenous rams. The study was conducted at the Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh during the period from May 2011 to April 2012. Forteen ram lambs (4~5 months) were randomly divided into two equal groups (n=7); supplemented vs control. The animals of control group were maintained on natural grazing. Along with natural grazing the supplemented group was on supplemented feeding. The concentrate supplementation (Wheat bran, Crushed maize, Soy bean meal, Fish meal, DCP powder, Vitamin mineral premix, Salt) was provided @ 300 g/head /day to the supplemented group. Body weight, scrotal circumference, BCS and libido index were measured weekly. Age, body weight and scrotal circumference at puberty were recorded. Semen was collected once in a weak using artificial vagina and chilled at $5^{\circ}C$ for 48h for evaluation. Concentrate supplementation did not influence (p>0.05) body condition score, age, weight, scrotal circumference at puberty and libido index. Final body weight (kg), growth rate (g/d), scrotal circumference (cm) and scrotal growth rate (mm/15d) were significantly (p<0.05) higher in supplemented group of rams compared to control. Volume, concentration, motility and membrane potentiality of spermatozoa were varied significantly (p<0.05) in supplemented and control groups. However, density, mass motility, viability and sperm with normal acrosome, midpiece and tail were not differed insignificantly (p>0.05) in different observation times. It was concluded that concentrate supplementation with free grazing improved weight and scrotal circumference gain and semen production with increased quality in indigenous ram.

Exfoliative Vaginal Cytology and Serum Progesterone during the Estrous Cycle of Indigenous Ewes in Bangladesh

Zohara, Begum Fatema,Azizunnesa, Azizunnesa,Islam, Md. Faruk,Alam, Md. Golam Shahi,Bari, Farida Yeasmin 韓國受精卵移植學會 2014 한국동물생명공학회지 Vol.29 No.2

A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, the pattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cycle length and duration of estrus were 15.8±0.12 days and 31.1±0.57 h respectively. The exfoliated epithelial cells were categorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages of parabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell type during estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated by neutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to 1.6±0.07 ng/ml. The length of diestrus was 5∼10 days with a range of mean progesterone level of 1.6±0.07 to 2.8±0.11 ng/ml. Progesterone levels increased significantly (p<0.01) after Day 5 and maximum level was 2.8±0.11 ng/ml observed on Day 10 of the estrous cycle. Thereafter it dropped rapidly to basal level of 0.11±0.04 ng/ml on Day 0 (p<0.01). These results indicate that the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine the reproductive stages of indigenous ewe.

Oestrus Synchronization with $Ovuprost^{(R)}$ and $Prostenol^{(R)}$ in the Indigenous Ewes of Bangladesh

Roy, Pantu Kumar,Zohara, Begum Fatema,Azizunnesa, Azizunnesa,Paul, Ashit Kumar,Bhuiyan, M.M.U.,Bari, Farida Yeasmin The Korean Society of Embryo Transfer 2014 한국동물생명공학회지 Vol.29 No.2

The present study was designed to observe the oestrus responses in the indigenous ewe induced by cloprostenol sodium manufactured by two different companies ($Ovuprost^{(R)}$, BOMAC, Newzealandand $Prostenol^{(R)}$, Techno, Bangladesh). Twelve local ewes were divided into 3 groups (n= 8). The ewes in Group I and II were induced by intramuscular injection of $100{\mu}g$ (0.4 ml) of cloprostenol sodium ($Ovuprost^{(R)}$ and $Prostenol^{(R)}$), respectively. The $2^{nd}$ injection in each group was given at 9 days apart. The ewes in Group III were kept as control for observing natural oestrus characteristics and comparing the responses with induced oestrus. Hundred and 75% ewes showed oestrus following $2^{nd}$ injection of $Ovuprost^{(R)}$ and $Prostenol^{(R)}$, respectively. The average time of onset of oestrus following $1^{st}$ and $2^{nd}$ injection of $Ovuprost^{(R)}$ and $Prostenol^{(R)}$ were $50.5{\pm}3.5$ vs $48.0{\pm}0.0h$ and $49.9{\pm}1.9$ vs $49.5{\pm}1.7h$, respectively. There was no significant difference between the two types of cloprostenol sodium group on the onset of oestrus. The average duration of oestrus was $27.5{\pm}0.7$ vs $27.5{\pm}0.0h$ and $25.9{\pm}3.3$ vs $24.2{\pm}0.3h$ in $Ovuprost^{(R)}$ and $Prostenol^{(R)}$ treated ewes, respectively. For natural oestrus, the duration of oestrus was $25.2{\pm}3.3h$. There was no significant difference among the cloprostenol sodium produced by two different companies and natural oestrous ewes on the duration of oestrus. The higher percentages of cornified cells were present in induced oestrus (90 and 85%) compared with natural oestrus (80%), although there was no significant difference among them. The pregnancy rates were 75, 66.7 and 100% in $Ovuprost^{(R)}$, $Prostenol^{(R)}$ and natural oestrous ewes, respectively. The above results indicate the suitability of using cloprostenol sodium for synchronization of oestrus in indigenous ewes in Bangladesh.

Exfoliative Vaginal Cytology and Serum Progesterone during the Estrous Cycle of Indigenous Ewes in Bangladesh

Begum Fatema Zohara,Azizunnesa,Md. Faruk Islam,Md. Golam Shahi Alam,Farida Yeasmin Bari 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.2

A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, thepattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cyclelength and duration of estrus were 15.8±0.12 days and 31.1±0.57 h respectively. The exfoliated epithelial cells werecategorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages ofparabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell typeduring estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated byneutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to 1.6±0.07 ng/ml. The length ofdiestrus was 5∼10 days with a range of mean progesterone level of 1.6±0.07 to 2.8±0.11 ng/ml. Progesterone levelsincreased significantly (p<0.01) after Day 5 and maximum level was 2.8±0.11 ng/ml observed on Day 10 of theestrous cycle. Thereafter it dropped rapidly to basal level of 0.11±0.04 ng/ml on Day 0 (p<0.01). These results indicatethat the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine thereproductive stages of indigenous ewe

Testicular and epididymal ultrasonography for the assessment of semen quality in the indigenous ram

Sarker Suchana,Zohara Begum Fatema,Azizunnesa,Islam Md. Faruk,Bari Farida Yeasmin 사단법인 한국동물생명공학회 2021 한국동물생명공학회지 Vol.36 No.4

The aims of this study were to measure the ultrasonographic biometry of genitalia of the indigenous rams and observe the relationship of biometry on semen parameters. The epididymal volume was significantly reduced (p < 0.01) after semen collection compared with before collection for both left and right part in all rams. The cumulative results showed that although there was no significant difference in length, width and volume of epididymis between before and after semen collection, however the values were lower after collection. The epididymal length was significantly correlated with epididymal volume (p < 0.01), semen motility (p < 0.05) and semen morphology (p < 0.01). Epididymal width was only significantly correlated with epididymal volume (p < 0.01) not with the semen parameters. Epididymal volume had a significant correlation only with semen morphology (p < 0.01).The scrotal circumference had the significant correlation with semen density, mass activity, concentration and motility (p < 0.01). The epididymis had the similar or slightly increased echogenicity as compared to the normal testis. During whole study, some white spots were found on testis which did not affect the semen quantity and quality. Significant variation was observed only for semen concentration and motility among the rams (p < 0.05). The overall normal morphology was 90.5 ± 4.6% with highest percentage of coiled tail abnormalities.

Testicular and epididymal ultrasonography for the assessment of semen quality in the indigenous ram

Suchana Sarker,Begum Fatema Zohara,Azizunnesa,Md. Faruk Islam,Farida Yeasmin Bari 한국동물생명공학회(구 한국동물번식학회) 2021 Journal of Animal Reproduction and Biotechnology Vol.36 No.4

The aims of this study were to measure the ultrasonographic biometry of genitalia of the indigenous rams and observe the relationship of biometry on semen parameters. The epididymal volume was significantly reduced (p < 0.01) after semen collection compared with before collection for both left and right part in all rams. The cumulative results showed that although there was no significant difference in length, width and volume of epididymis between before and after semen collection, however the values were lower after collection. The epididymal length was significantly correlated with epididymal volume (p < 0.01), semen motility (p < 0.05) and semen morphology (p < 0.01). Epididymal width was only significantly correlated with epididymal volume (p < 0.01) not with the semen parameters. Epididymal volume had a significant correlation only with semen morphology (p < 0.01).The scrotal circumference had the significant correlation with semen density, mass activity, concentration and motility (p < 0.01). The epididymis had the similar or slightly increased echogenicity as compared to the normal testis. During whole study, some white spots were found on testis which did not affect the semen quantity and quality. Significant variation was observed only for semen concentration and motility among the rams (p < 0.05). The overall normal morphology was 90.5 ± 4.6% with highest percentage of coiled tail abnormalities.