Pregnancy diagnosis in goat by using vaginal cytology and trans-abdominal ultrasonography

Md. Aziz Ali,Md. Faruk Islam,S M Latifur Rahman,Begum Fatema Zohara 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.4

The present study was conducted in a goat farm for pregnancy diagnosis by using vaginal cytology and B-mode real time ultrasound using 5 MHz probe by transabdominal method. Seventeen pregnant does were used for this study. The objective of the study was to determine the earliest day of pregnancy and describe the chronological characteristics of pregnancy from day 22 to day 40 for vaginal cytology and day 25 to day 60 for ultrasonography of gestation. The differences among the average percentage of cell value in different age of pregnancy were significant (p < 0.05). The average percentage of intermediate cells (81.12%) was also significantly (p < 0.05) higher than superficial (9.41%), keratinized (7.10%) and neutrophil (2.61%) on 22-40th days of pregnancy. In case of real time B-mode ultrasonography, the gestational sac was observed only in three does out of seventeen (17.6 %) at 25-30 days whereas the placentomes and heart beat of the foetus were first detected at 31-35 days in six does (35.3%). The foetal leg buds were first visualized at 36-40 days in four does (23.5%) whereas the foetal vertebral column was first observed at 36-40 days of gestation in only three does (17.6%). In conclusion, vaginal cytology and trans-abdominal ultrasonography can be used for detection of early pregnancy in does.

Effects of Periparturient Anthelmintic Treatment on the Milk Yield in Cows

Md. Faruk Islam,Begum Fatema Zohara,Nurjahan Begum,Md. Golam Shahi Alam 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.2

This study was conducted to determine the efficacy of anthelmintics against gastrointestinal parasitic infestation inperiparturient dairy cows and its effect on milk yield and quality. Sixty pregnant cows of 1st & 2nd parity weredivided into four groups. The efficacy of anthelmintic treatment was evaluated by counting faecal egg per gram (EPG)compared with pre-treatment values. The milk yield of each cow was recorded in pre and post treatment lactations. Cows of group A were treated with Nitroxynil 10 mg/kg body weight subcutaneously 30 days before parturition, groupB were treated with combination of triclabendazole and levamisole 19.5 mg/kg body weight orally at calving andgroup C treated with Endex® at calving and 42 days after. The mean change in EPG 14 days after treatment wassignificantly higher (p<0.05) in treated (79.1%) cows than control. Average milk yield of group C (2.8 ± 0.8) wassignificantly higher (p<0.01) than group A (2.6 ± 0.7). Similarly, the average milk yield in all the treated cows wassignificantly (p<0.01) higher in treated lactation (2.5 ± 0.7) than in the previous lactation (2.2 ± 0.7). The averagemilk yield in all treated cows was significantly (p<0.01) higher than the control cows. Although, milk yields werehigher in second parity than the first, there was no significant difference (p>0.05). The milk protein percentage wassignificantly higher (p<0.05) in treated group than the control group. Therefore, it may be concluded that periparturientanthelmintic treatment effectively reduced the gastrointestinal parasitic load and improved milk yield.

Effects of Periparturient Anthelmintic Treatment on the Milk Yield in Cows

Islam, Faruk Md.,Zohara, Begum Fatema,Begum, Nurjahan,Alam, Md. Golam Shahi 韓國受精卵移植學會 2014 한국동물생명공학회지 Vol.29 No.2

This study was conducted to determine the efficacy of anthelmintics against gastrointestinal parasitic infestation in periparturient dairy cows and its effect on milk yield and quality. Sixty pregnant cows of 1st & 2nd parity were divided into four groups. The efficacy of anthelmintic treatment was evaluated by counting faecal egg per gram (EPG) compared with pre-treatment values. The milk yield of each cow was recorded in pre and post treatment lactations. Cows of group A were treated with Nitroxynil 10 mg/kg body weight subcutaneously 30 days before parturition, group B were treated with combination of triclabendazole and levamisole 19.5 mg/kg body weight orally at calving and group C treated with Endex® at calving and 42 days after. The mean change in EPG 14 days after treatment was significantly higher (p<0.05) in treated (79.1%) cows than control. Average milk yield of group C (2.8 ± 0.8) was significantly higher (p<0.01) than group A (2.6 ± 0.7). Similarly, the average milk yield in all the treated cows was significantly (p<0.01) higher in treated lactation (2.5 ± 0.7) than in the previous lactation (2.2 ± 0.7). The average milk yield in all treated cows was significantly (p<0.01) higher than the control cows. Although, milk yields were higher in second parity than the first, there was no significant difference (p>0.05). The milk protein percentage was significantly higher (p<0.05) in treated group than the control group. Therefore, it may be concluded that periparturient anthelmintic treatment effectively reduced the gastrointestinal parasitic load and improved milk yield.

Pregnancy diagnosis in goat by using vaginal cytology and trans-abdominal ultrasonography

Ali, Md. Aziz,Islam, Md. Faruk,Rahman, SM Latifur,Zohara, Begum Fatema The Korean Society of Animal Reproduction and Biot 2020 한국동물생명공학회지 Vol.35 No.4

The present study was conducted in a goat farm for pregnancy diagnosis by using vaginal cytology and B-mode real time ultrasound using 5 MHz probe by transabdominal method. Seventeen pregnant does were used for this study. The objective of the study was to determine the earliest day of pregnancy and describe the chronological characteristics of pregnancy from day 22 to day 40 for vaginal cytology and day 25 to day 60 for ultrasonography of gestation. The differences among the average percentage of cell value in different age of pregnancy were significant (p < 0.05). The average percentage of intermediate cells (81.12%) was also significantly (p < 0.05) higher than superficial (9.41%), keratinized (7.10%) and neutrophil (2.61%) on 22-40th days of pregnancy. In case of real time B-mode ultrasonography, the gestational sac was observed only in three does out of seventeen (17.6 %) at 25-30 days whereas the placentomes and heart beat of the foetus were first detected at 31-35 days in six does (35.3%). The foetal leg buds were first visualized at 36-40 days in four does (23.5%) whereas the foetal vertebral column was first observed at 36-40 days of gestation in only three does (17.6%). In conclusion, vaginal cytology and trans-abdominal ultrasonography can be used for detection of early pregnancy in does.

Effect of rhizosphere microbiome on different crop growing fields in various rice cultivars and its molecular approaches for sustainable agro-ecosystem

Sharmin Nahar,Md. Faruk Hasan,Sikdar Biswanath,Md. Asadul Islam 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.5

The present investigation was carried out to investigate the efects of rhizosphere bacteria on growth and rice yields. Also, isolation and identifcation of rhizospheric bacterial treatments rhizosphere soil, mixed bulk, and fertilized soils. Soil analysis showed a maximum pH of 8.46 in rhizosphere soil of Sada dhepa. Nitrogen content of the rhizosphere soil of Latif sayil showed 0.18% maximum result. In-plant growth and yield, 87.37 cm leaf length, 28.83 cm panicle length, and 2.32 gm 100 seed weight in rhizosphere soil of Latif sayil were observed. Five diferent rhizobial bacteria Rhizobium tropici, Rhizobium leguminosarum, Rhizobium freirei, Rhizobium oryzae, Bacillus subtilis were isolated from the rhizosphere. Mixed treatment on Azospirillum largimobile, Azotobacter chroococcum, Rhizobium leguminosarum, Pseudomonas fuorescens were isolated from the rhizosphere. Mixed treatment was signifcantly similar to Rhizobium tropici and Azotobacter chroococcum by a maximum of 82.32% and 86.19%, respectively. Our results indicate that selected rhizobial strains promote rice growth and yields that could be harnessed to practical beneft for the farmer and are consistent with sustainable agricultural practices.

Mining the Proteome of Fusobacterium nucleatum subsp. nucleatum ATCC 25586 for Potential Therapeutics Discovery: An In Silico Approach

Abdul Musaweer Habib,Md. Saiful Islam,Md. Sohel,Md. Habibul Hasan Mazumder,Mohd. Omar Faruk Sikder,Shah Md. Shahik 한국유전체학회 2016 Genomics & informatics Vol.14 No.4

The plethora of genome sequence information of bacteria in recent times has ushered in many novel strategies for antibacterial drug discovery and facilitated medical science to take up the challenge of the increasing resistance of pathogenic bacteria to current antibiotics. In this study, we adopted subtractive genomics approach to analyze the whole genome sequence of the Fusobacterium nucleatum, a human oral pathogen having association with colorectal cancer. Our study divulged 1,499 proteins of F. nucleatum, which have no homolog’s in human genome. These proteins were subjected to screening further by using the Database of Essential Genes (DEG) that resulted in the identification of 32 vitally important proteins for the bacterium. Subsequent analysis of the identified pivotal proteins, using the Kyoto Encyclopedia of Genes and Genomes (KEGG) Automated Annotation Server (KAAS) resulted in sorting 3 key enzymes of F. nucleatum that may be good candidates as potential drug targets, since they are unique for the bacterium and absent in humans. In addition, we have demonstrated the three dimensional structure of these three proteins. Finally, determination of ligand binding sites of the 2 key proteins as well as screening for functional inhibitors that best fitted with the ligands sites were conducted to discover effective novel therapeutic compounds against F. nucleatum.

Mining the Proteome of Fusobacterium nucleatum subsp. nucleatum ATCC 25586 for Potential Therapeutics Discovery: An In Silico Approach

Habib, Abdul Musaweer,Islam, Md. Saiful,Sohel, Md.,Mazumder, Md. Habibul Hasan,Sikder, Mohd. Omar Faruk,Shahik, Shah Md. Korea Genome Organization 2016 Genomics & informatics Vol.14 No.4

The plethora of genome sequence information of bacteria in recent times has ushered in many novel strategies for antibacterial drug discovery and facilitated medical science to take up the challenge of the increasing resistance of pathogenic bacteria to current antibiotics. In this study, we adopted subtractive genomics approach to analyze the whole genome sequence of the Fusobacterium nucleatum, a human oral pathogen having association with colorectal cancer. Our study divulged 1,499 proteins of F. nucleatum, which have no homolog's in human genome. These proteins were subjected to screening further by using the Database of Essential Genes (DEG) that resulted in the identification of 32 vitally important proteins for the bacterium. Subsequent analysis of the identified pivotal proteins, using the Kyoto Encyclopedia of Genes and Genomes (KEGG) Automated Annotation Server (KAAS) resulted in sorting 3 key enzymes of F. nucleatum that may be good candidates as potential drug targets, since they are unique for the bacterium and absent in humans. In addition, we have demonstrated the three dimensional structure of these three proteins. Finally, determination of ligand binding sites of the 2 key proteins as well as screening for functional inhibitors that best fitted with the ligands sites were conducted to discover effective novel therapeutic compounds against F. nucleatum.

Exfoliative Vaginal Cytology and Serum Progesterone during the Estrous Cycle of Indigenous Ewes in Bangladesh

Begum Fatema Zohara,Azizunnesa,Md. Faruk Islam,Md. Golam Shahi Alam,Farida Yeasmin Bari 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.2

A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, thepattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cyclelength and duration of estrus were 15.8±0.12 days and 31.1±0.57 h respectively. The exfoliated epithelial cells werecategorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages ofparabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell typeduring estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated byneutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to 1.6±0.07 ng/ml. The length ofdiestrus was 5∼10 days with a range of mean progesterone level of 1.6±0.07 to 2.8±0.11 ng/ml. Progesterone levelsincreased significantly (p<0.01) after Day 5 and maximum level was 2.8±0.11 ng/ml observed on Day 10 of theestrous cycle. Thereafter it dropped rapidly to basal level of 0.11±0.04 ng/ml on Day 0 (p<0.01). These results indicatethat the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine thereproductive stages of indigenous ewe

Exfoliative Vaginal Cytology and Serum Progesterone during the Estrous Cycle of Indigenous Ewes in Bangladesh

Zohara, Begum Fatema,Azizunnesa, Azizunnesa,Islam, Md. Faruk,Alam, Md. Golam Shahi,Bari, Farida Yeasmin 韓國受精卵移植學會 2014 한국동물생명공학회지 Vol.29 No.2

A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, the pattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cycle length and duration of estrus were 15.8±0.12 days and 31.1±0.57 h respectively. The exfoliated epithelial cells were categorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages of parabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell type during estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated by neutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to 1.6±0.07 ng/ml. The length of diestrus was 5∼10 days with a range of mean progesterone level of 1.6±0.07 to 2.8±0.11 ng/ml. Progesterone levels increased significantly (p<0.01) after Day 5 and maximum level was 2.8±0.11 ng/ml observed on Day 10 of the estrous cycle. Thereafter it dropped rapidly to basal level of 0.11±0.04 ng/ml on Day 0 (p<0.01). These results indicate that the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine the reproductive stages of indigenous ewe.

Testicular and epididymal ultrasonography for the assessment of semen quality in the indigenous ram

Sarker Suchana,Zohara Begum Fatema,Azizunnesa,Islam Md. Faruk,Bari Farida Yeasmin 사단법인 한국동물생명공학회 2021 한국동물생명공학회지 Vol.36 No.4

The aims of this study were to measure the ultrasonographic biometry of genitalia of the indigenous rams and observe the relationship of biometry on semen parameters. The epididymal volume was significantly reduced (p < 0.01) after semen collection compared with before collection for both left and right part in all rams. The cumulative results showed that although there was no significant difference in length, width and volume of epididymis between before and after semen collection, however the values were lower after collection. The epididymal length was significantly correlated with epididymal volume (p < 0.01), semen motility (p < 0.05) and semen morphology (p < 0.01). Epididymal width was only significantly correlated with epididymal volume (p < 0.01) not with the semen parameters. Epididymal volume had a significant correlation only with semen morphology (p < 0.01).The scrotal circumference had the significant correlation with semen density, mass activity, concentration and motility (p < 0.01). The epididymis had the similar or slightly increased echogenicity as compared to the normal testis. During whole study, some white spots were found on testis which did not affect the semen quantity and quality. Significant variation was observed only for semen concentration and motility among the rams (p < 0.05). The overall normal morphology was 90.5 ± 4.6% with highest percentage of coiled tail abnormalities.