A genetic variation in microRNA target site of <i>KRT81</i> gene is associated with survival in early-stage non-small-cell lung cancer

Lee, S. Y.,Choi, J. E.,Jeon, H. S.,Hong, M. J.,Choi, Y. Y.,Kang, H. G.,Yoo, S. S.,Lee, E. B.,Jeong, J. Y.,Lee, W. K.,Lee, J.,Cha, S. I.,Kim, C. H.,Kim, Y. T.,Jheon, S.,Son, J. W.,Park, J. Y. Oxford University Press 2015 ANNALS OF ONCOLOGY Vol.26 No.6

<P>In this study, <I>KRT81</I> rs3660G>C was associated with survival of patients with NSCLC after surgical resection. Mechanistic study suggested that the G-to-C change caused reduced binding efficiency of miRNA, leading to decreased translational repression, thereby increased <I>KRT81</I> expression. The <I>KRT81</I> rs3660G>C may be a useful prognostic biomarker in early-stage NSCLC patients.</P><P><B>Background</B></P><P>MicroRNAs (miRNAs) have a key role in carcinogenesis through negative regulation of their target genes. Therefore, genetic variations in miRNAs or their target sites may affect miRNA–mRNA interactions, thereby result in altered expression of target genes. This study was conducted to investigate the associations between single-nucleotide polymorphisms (SNP) located in the miRNA target sites (poly-miRTSs) and survival of patients with early-stage non-small-cell lung cancer (NSCLC).</P><P><B>Methods</B></P><P>Using public SNP database and miRNA target sites prediction program, 354 poly-miRTSs were selected for genotyping. Among these, 154 SNPs applicable to Sequenom's MassARRAY platform were investigated in 357 patients. A replication study was carried out on an independent patient population (<I>n</I> = 479). <I>Renilla</I> luciferase assay and reverse transcription-polymerase chain reaction were conducted to examine functional relevance of potentially functional poly-miRTSs.</P><P><B>Results</B></P><P>Of the 154 SNPs analyzed in a discovery set, 14 SNPs were significantly associated with survival outcomes. Among these, <I>KRT81</I> rs3660G>C was found to be associated with survival outcomes in the validation cohort. In the combined analysis, patients with the rs3660 GC + CC genotype had a significantly better overall survival compared with those with GG genotype [adjusted hazard ratio (aHR) for OS, 0.65; 95% confidence interval (CI) 0.50–0.85; <I>P</I> = 0.001]. An increased expression of the reporter gene for the C allele of rs3660 compared with the G allele was observed by luciferase assay. Consistently, the C allele was associated with higher relative expression level of <I>KRT81</I> in tumor tissues.</P><P><B>Conclusion</B></P><P>The rs3660G>C affects KRT81 expression and thus influences survival in early-stage NSCLC. The analysis of the rs3660G>C polymorphism may be useful to identify patients at high risk of a poor disease outcome.</P>

Submergence-inducible and circadian rhythmic basic helix-loop-helix protein gene in Nicotiana tabacum

Ko, D.K.,Lee, M.O.,Hahn, J.S.,Kim, B.g.,Hong, C.B. G. Fischer 2009 Journal of plant physiology Vol.166 No.10

Submergence stress leads to diverse changes in transcription and translation of genes involved in developmental and physiological metabolisms of plants. The basic helix-loop-helix (bHLH) protein family is one of the largest transcriptional factor families in plants, and has been shown to play pivotal roles in diverse biological responses. However, there has been no report on bHLH protein related to submergence stress response. In this study, a novel bHLH gene, NtbHLH, was isolated from tobacco (Nicotiana tabacum) by differential screening of a submergence-stress-induced cDNA library. NtbHLH cDNA is 1027bp in length, with an open reading frame (ORF) of 702 nucleotides encoding 233 amino acid residues that contain the bHLH domain. RNA-blot analyses showed that transcription of NtbHLH was induced by submergence stress, while cold, heat shock, and drought decreased its expression. The gene expression was down-regulated by gibberellins, but ABA and ethylene seemed not to affect it. It was also apparent that NtbHLH expression follows circadian rhythmicity. The electrophoretic mobility shift and chemical cross-linking assays showed that NtbHLH specifically binds to G-box and forms homo-dimers.

B-ISDN 망의 AESA DCC, ICD 주소체계와 루팅

김상현,오문균,홍성백,Kim, S.H.,Oh, M.G.,Hong, S.B. 한국전자통신연구원 1999 전자통신동향분석 Vol.14 No.5

본 고에서는 B-ISDN 망의 NNI 프로토콜로 B-ISUP과 PNNI가 동시에 수용되는 공중 망에서 ITU-T E.164 주소체계 사용에 따른 장.단점을 살펴보고, 공중망 교환기에 ATM Forum 신호 권고안을 수용하면서 지금까지 주로 사설 ATM 망에서 사용되어 온 AESA DCC, ICD/IOTA 주소체계를 공중망에 수용하기 위한 검토사항, 필요성, 수용방법을 기술하고, 국내에서 구축중인 B-ISDN 망의 AESA DCC, ICD 주소형태(address format) 및 각 주소계위(address hierarchy)의 의미, 이를 지원하기 위한 공중망 교환기의 기능에 대하여 기술하고자 한다.

工業高等學校 電子科 敎育方向의 設定

洪鳳植,王鎭錫,吳承燁,金太均,林在鳳,朴東徹,沈殷燮 충남대학교 공업교육연구소 1979 論文集 Vol.2 No.2

In the last ten years there has been a dramatic change in the electrical industry regarding its technology and capability. VLSI circuits technologies have brought about the develoment of computers, digital communications, digital control systems, and others. The microprocessor now is appearing in the simplest appliances as well as in complex systems, permeating all of engineering. This paper is presented as a proposal for a more improved education courses for technical high school students preparing to enter this dynamic electronic industry. The current curricula, and their syllabuses, of the electronic deparments of typical technical high schools were analyzed, and their teachers and instructors, as well as technicians in electronic companies, were consulted about the education courses. The results of this study are follow: 1. The fields of digital circuits and systems using IC and LSI should be emphasized in the current curricula and the subject of microprocessor applications should be added. 2. The current ratio of the theoretical; subject hours vs laboratory /shop practice hours should be rearranged at about same ratio through effective laboratory systems. 3. A retraining course for experimental instructors and supervisors should be provided, similar to that provided for teachers. 4. Technical high school education should avoid the erroneous philosophy of training the student only sufficiently to pass the qualification examination.

중·고등학교 여학생의 월경 전후기 불편감에 따른 월경태도 및 성 역할 고정관념의 관계

강슬기,김효민,박서희,백채영,심자영,우한빛,이빛나,이채은,홍지수 이화여자대학교 간호과학대학 2014 이화간호학회지 Vol.- No.48

Purpose: The purpose of this study was to provide the basis for program helping adolescent girls to form correct attitude toward menstruation and gender role stereotype by identifying the relationship among menstrual distress, attitudes toward menstruation and gender role stereotype. Method: The 392 participants of this study were selected among students of 5 middle schools and 3 high schools located in Seoul and Gyeong-gi province. Data were collected from 16 September to 23 September, 2013. The students were asked to answer the questionnaires consisting of MDQ(Menstrual Distress Questionnaire), KGES-A(Korean Gender Egalitarianism Scales for Adolescents), and menstrual attitude measurement scale developed by Min in 2003. Descriptive statistics, independent t-test and One way ANOVA were used to analyze the data with SPSS 21.0. Results: The results of this study were as follows: 1. High school students were more vulnerable to pre and post menstrual discomfort and tend to accept menstruation statically than middle school students(p=.009). 2. Students who did not receive regular sexuality education had private attitudes toward menstruation(p=.002), were emotionally more sensitive(p=.002) than the other group. 3. Students, who had higher menstrual discomfort, were more emotionally sensitive toward menstruation(p<.001), had variety of menstrual symptoms(p<.001), actively managing menstrual symptoms(p<.001), and accepted menstruation statically(p=.002) than the lower group. 4. There was no statistically significant relationship between gender role stereotype and pre and post menstrual discomfort. Conclusion: It was suggested that effective education program should be developed and implemented periodically in order to help adolescent school girls to understand pre and post menstrual discomfort and manage menstrual symptoms by themselves. Also, professional and systematic studies should follow up with larger population and more specific general characteristics.

Genetic Polymorphisms of the Bovine NOV Gene Are Significantly Associated with Carcass Traits in Korean Cattle

Kim, B.S.,Kim, S.C.,Park, C.M.,Lee, S.H.,Cho, S.H.,Kim, N.K.,Jang, G.W.,Yoon, D.H.,Yang, B.S.,Hong, S.K.,Seong, H.H.,Choi, B.H. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.6

The objective of this study was to investigate single nucleotide polymorphisms (SNPs) in the bovine nephroblastoma overexpressed (NOV) gene and to evaluate whether these polymorphisms affect carcass traits in the Korean cattle population. We resequenced to detect SNPs from 24 unrelated individuals and identified 19 SNPs within the full 8.4-kb gene, including the 1.5-kb promoter region. Of these 19 SNPs, four were selected for genotyping based on linkage disequilibrium (LD). We genotyped 429 steers to assess the associations of these four SNPs with carcass traits. Statistical analysis revealed that g.7801T>C and g.8379A>C polymorphisms in the NOV gene were associated with carcass weight (p = 0.012 and 0.008, respectively), and the g.2005A>G polymorphism was associated with the back fat thickness (BF) trait (p = 0.0001). One haplotype of the four SNPs (GGTA) was significantly associated with BF (p = 0.0005). Our findings suggest that polymorphisms in the NOV gene may be among the important genetic factors affecting carcass yield in beef cattle.

Hierarchical MnO₂ nanosheet arrays on carbon fiber for high-performance pseudocapacitors

Yang, M.,Hong, S.B.,Choi, B.G. Elsevier Sequoia 2015 Journal of Electroanalytical Chemistry Vol.759 No.2

<P>A hierarchical porous structure of MnO2 nanosheet arrays is synthesized on three-dimensional surface of carbon fiber paper (CFP). As-synthesized MnO2/CFP is thoroughly characterized by transmission electron microscopy, scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The direct deposition of MnO2 enables a good electrical connection to the CFP, and thus eliminates the need for polymeric binders and carbon additives. Moreover, the unique structure with a large surface area enables efficient and rapid ion and electron transfer in electrochemical processes. When testing as an electrode for supercapacitors, the MnO2/CFP shows a specific capacitance (204 F/g), rate capability (75% retention), and good cycling life (similar to 100% retention after 1000 cycles). (C) 2015 Elsevier B.V. All rights reserved.</P>

여대생의 우울, 스트레스와 문제도박과의 관계

하주희,차민주,조에스더,조세빈,홍소리,황정민,박지윤,강하영,노규상 이화여자대학교 간호과학대학 2015 이화간호학회지 Vol.- No.49

Purpose: The purpose of this study was to learn about problem-gambling level, depression and stress among female college students. Method: In this cross-sectional design study, a convenient sample of 325 female college students were recruited between September and October, 2014. Measurement used for this study were the CPGI(Canadian Problem Gambling Index), the CES-D(Center for Epidemiologic Studies Depression Scale), and the Life stress scale for college students. Data were statistically analyzed using descriptive statistics, t-test, ANOVA and chi-square test. Result: About 58.5 percent of the participants had experience of gambling; lottery was the most frequently used gambling among participants. 36 participants(11.1%) were problem gamblers. Relationship of smoking status to problem gambler group was statistically significant. There were statistically significant relationships among problem-gambling level(Non-problem, Low-risk, Moderate-risk, High-risk), depression and stress. The problem gambler group had significantly higher trait stress scores compared to the normal group. Conclusion: Problem gambling was a risk factor for both depression and stress among female college students. The proposals of this study are as follows; first, in depth-research are required with more expanded sampling as sample of this study was conveniently gathered, it is hard to generalize the result. Second, a problem gambling prevention program is recommended for low-risk problem gamblers as they have risk to become problem gambler later. Third, nursing interventions for problem gamblers should be investigated with regard to depression and stress level.

인터넷 기반 정보거래시스템에 관한 연구

홍기채,문병주,Hong, G.C.,Moon, B.J. 한국전자통신연구원 2002 전자통신동향분석 Vol.17 No.6

본 고에서는 인터넷 기반에서 디지털 콘텐츠 유료화를 위한 정보거래시스템의 구현을 목표로 디지털 콘텐츠 유료화에 관한 최신의 기술 동향 및 실제적인 시스템 구현을 위한 구성 요소들을 살펴 보고자 한다. 이에 디지털 콘텐츠 유료화를 위해서 반드시 필요되는 디지털 콘텐츠 보안 기술, 과금(빌링)시스템, 전자 결제 방식 및 PG, 디지털 콘텐츠 전송기술, 디지털 콘텐츠 저작권 보호 기술 등에 대한 내용 중심으로 고찰할 것이다. 또한 이를 기반으로 구현하고자 하는 정보거래시스템의 커머스 모델로는 C-to-C 개념으로 인터넷 기반의 정보 생성자와 정보 이용자를 직접 연계하고, 정보 이용에 대한 지불 처리가 가능하도록 하여 기존의 C-to-B 및 B-to-B 기반 전자상거래에서 C-to-C 기반 전자상거래 개념 도입을 통한 정보거래 기반 및 정보 자체에 대한 인터넷 거래 활성화를 유도할 수 있는 기술적 기반 환경을 조성하고, 실제적으로 활용이 가능한 프로토타입 모델을 설계하고자 한다.

Effect of Growth Performance and Disease Occurrence on Castration and Injection time of Recombinant Bovine Somatotropin(rbST) in Holstein Bulls

Kweon, O. G 韓國酪農學會 1997 韓國酪農學會誌 Vol.19 No.3