ICR 마우스를 이용한 삼소음 및 발효삼소음의 급성독성 연구

이주혜,황윤환,이지혜,임남희,조원경,마진열,Lee, Ju-Hye,Hwang, Youn-Hwan,Lee, Ji-Hye,Yim, Nam-Hui,Cho, Won-Kyung,Ma, Jin-Yeul 대한한의학방제학회 2011 大韓韓醫學方劑學會誌 Vol.19 No.2

Objectives : This study aims to investigate the acute oral toxicity and safety of Samsoeum (Shensuyin) extracts and fermented Samsoeum extracts. Methods : For that objective, we used ICR mice. ICR mice were administerd orally with dosage of 1250mg/kg, 2500mg/kg and 5000mg/kg of Samsoeum extracts and fermented Samsoeum extracts. Results : We daily examined number of deaths, clinical signs, body weights and gross findings for 2 weeks. 1. The results of acute oral toxicity using ICR mice showed that LD50 of value over 5000 mg/kg. 2. Samsoeum extracts and fermented Samsoeum extracts not affect on bodyweight gross findings of ICR mice. 3. The results of Serum chemistry analysis and Complete Blood Count(CBC) through the autopsy were showed normal range values. Conclusions : Samsoeum extracts and fermented Samsoeum extracts did not show any toxic effects in ICR mice. And oral LD50 value was over 5000mg/kg in ICR mice and it is very safe for ICR mice.

열처리방법에 따른 한지형 및 난지형 마늘의 영양성분 변화

이주혜,이지윤,황진봉,남진식,이준수,김소민,한혜경,최용민,김세나,김행란,Lee, Ju-Hye,Lee, Jiyoon,Whang, Jinbong,Nam, Jin-Sik,Lee, Junsoo,Kim, So-Min,Han, Hye-Kyung,Choi, Youngmin,Kim, Se-Na,Kim, Haeng Ran 한국식품조리과학회 2016 한국식품조리과학회지 Vol.32 No.3

Purpose: To provide the public with nutritional information on consumption types of garlic, we evaluated the influence of heat treatment method on the nutritional contents of different species of garlic. Methods: We determined the content of general components, minerals, vitamins, and fatty acids in each species of garlic produced in Seosan and Goheung by heating with blanching or microwave roasting. Results: The results of the two-way analysis of variance test indicated that the species in particular, as well as method of heat treatment and interaction, had an influence on nutritional content. The moisture and crude fat content was higher in Southern type garlic than in Northern type garlic, while crude proteins, crude ash, and dietary fibers were more abundant in Northern type garlic than in Southern type garlic. With regard to the total mineral content, K, P, Mg, and Ca were the main components in Northern type garlic and Southern type garlic. Moreover, unsaturated fatty acids showed high levels in both Northern type garlic and Southern type garlic, with more abundant linoleic acid. Overall, Northern type garlic showed a higher content of minerals and fatty acids, while more vitamin B was present in Southern type garlic. In addition, the results indicated that the content of general components (dietary fiber excluded) was increased in both Northern type garlic and Southern type garlic upon heat treatment. Blanching resulted in increased mineral and fatty acid content in Southern type garlic and decreased content in Northern type garlic, indicative of species differences; conversely, microwave roasting contributed to an increase in the content in both Northern type garlic and Southern type garlic. Conclusion: The nutritional content of garlic is more dependent on species than the cooking method, and grilling is associated with less nutrient loss than blanching.

생애주기에 따른 갈색거저리의 식품성분 변화

이주혜,이지윤,황진봉,남진식,한혜경,김소민,임정연,최용민,김행란,김세나,Lee, Ju-Hye,Lee, Jiyoon,Whang, Jinbong,Nam, Jin-Sik,Han, Hye-Kyung,Kim, So-Min,Im, Jeong Yeon,Choi, Youngmin,Kim, Haeng Ran,Kim, Se-Na 한국식품조리과학회 2016 한국식품조리과학회지 Vol.32 No.5

Purpose: This study was aimed to compare the nutritional content of the life stages of Tenebrio molitor. Methods: Nutritional element analysis comprised 12 minerals, 18 amino acids, 4 vitamins, and 12 fatty acids in larval, pupal, and adult stages. Nutritional element content was expressed as a percentage of 100 g of sample and determined in raw and freeze-dried samples to assess the food value of the insect. Results: Moisture, crude protein, and crude fat contents in raw samples were 63.5%, 23.19%, and 3.96%, respectively. Moisture content was significantly higher in the adult stage than in larval and pupal stages, whereas crude fat and crude ash contents were lower. Mineral composition of the mealworm showed K, P, and Mg in decreasing order of content, with no differences among life stages. Total amino acid content increased from adult, pupal to larval stages, in order with each stage's growth, without difference in amino acid composition. Seventy-six to 78% of total fatty acid corresponded to unsaturated fatty acids, with oleic acid as the most abundant. In addition, fatty acid content was high in the adult stage, and clear differences were observed between life stages. Finally, vitamins B1 and B3 showed similar content between life stages, whereas vitamin B2 showed a higher content the adult than larval and pupal stages. A higher folate content was observed in pupal and adult stages than in the larval stage. Although freeze-dried samples showed a higher nutritional content than raw samples, both samples were similar in composition and content between life stages, suggesting the suitability of freeze-dried samples for use as food materials. Conclusion: These results may provide a basis for more diversified industrial applications of the Tenebrio molitor.

학업중단 위기청소년의 대안교육 경험 사례연구 : 문화예술교육 활동 중심으로

이주혜(Lee Ju Hye),김명인(Kim Myeong In),박성옥(Park Sung Ouk) 학습자중심교과교육학회 2021 학습자중심교과교육연구 Vol.21 No.2

The purpose of this study is to examine what students at risk for school dropout experience through cultural and art education activities as alternative education and what the meaning of those experiences is. This study tried to find out the significance of alternative education regarding school dropout prevention. The researchers conducted a study on 10 middle and high school students at risk for school dropout, grounding on the analysis and interpretation method that Stake(1955) proposed. The subjects of this study were students at risk, who was selected by professional counselors in schools in the S city, and adolescents in the youth welfare center Kkumdream and voluntarily participated in cultural and art education activities. The study duration was 11 weeks in total, from August 20 to November 4, 2019; 3 times a week, 4-5 hours each. Per the results, the students went through cultural and art education activities experienced restoration of confidence, change in self-efficacy, improved self-esteem, novel interpersonal relationships, improved communication skills, sense of achievement, academic motivation, expecting the future, self-curing. Regarding the meaning of the experience, students experienced discovery of internal resources, recognition of prospects in themselves, improved sociability, respecting themselves and others , and active changes in attitude.‘ Based on this study, we discussed the significance of cultural and artistic education activities as an alternative education for the prevention of academic interruption, and ways to improve and cope with the system of educational sites. 본 연구의 목적은 학업중단 위기청소년이 대안 교육으로써의 문화예술교육활동을 통해 무엇을 경험하고 그 경험은 어떤 의미를 주는지 알아보는 데 있다. 이 연구를 위해 Stake(1955)가 제안한 분석과 해석방법을 기반으로 학업중단 위기에 놓인 청소년의 문화예술교육활동 경험과 그 의미에 대해 심층적으로 이해할 수 있는 질적 사례 연구 방법으로 진행하였다. 연구대상은 S시 관내 중 · 고등학교 전문상담교사가 선별한 학업중단 위기 위험 군에 속하는 학생과 청소년 복지센터 ‘꿈드림’ 소속 청소년 중, 문화예술교육활동에 자발적으로 지원하여 활동한 10명을 대상으로 연구를 진행하였다. 연구기간은 2019년 8월 20일부터 11월 4일까지 11주 동안 주 3회 매 회 4∼5시간 진행하였다. 연구 결과는 학업중단 위기 청소년이 문화예술교육활동을 통해 ‘자신감 회복’ ‘자기효능감의 변화’ ‘자존감 증진’ ‘새로운 대인관계’ ‘의사소통능력 향상’ ‘성취감’ ‘학업 동기’ ’미래 기대’ ‘자기 치유’를 경험하고, 경험의 의미로는 ‘내적자원 발견’ ‘잠재성의 인식’ ‘사회성 향상’ ‘자기, 타인 존중’ ‘능동적 태도의 변화’가 도출되었다. 이 연구를 바탕으로 학업중단 예방을 위한 대안 교육으로써 문화예술교육활동의 의의, 교육 현장의 제도개선과 대처 방안에 대해 논의하였다.

오이 발효음료가 만성적으로 에탄올을 급여한 흰쥐의 에탄올 대사와 항산화방어계에 미치는 영향

이해인(Hae-In Lee),서권일(Kwon-Il Seo),이진(Jin Lee),이점숙(Jeom-Sook Lee),홍성민(Sung-Min Hong),이주혜(Ju-Hye Lee),김명주(Myung-Joo Kim),이미경(Mi-Kyung Lee) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.8

본 연구는 다량으로 폐기되는 오이를 이용하기 위하여 개발한 오이 발효원액을 주원료로 제조한 숙취해소 음료의 간보호 효능을 검증하기 위하여 만성적으로 에탄올을 섭취시킨 흰쥐에서 에탄올 대사, 항산화 방어계, 간독성 관련지표 및 지질함량 변화를 살펴보았다. 실험동물은 4 주령의 수컷 SD계 흰쥐 24마리를 1주간 고형식이로 적응시킨 후 난괴법에 의하여 에탄올대조군(Control) 및 에탄올 섭취 흰쥐에게 헛개열매 추출물을 주원료로 하여 개발한 숙취해소 물질인 SKM 급여군(SKM) 또는 SKM을 함유한 오이 발효음료 급여군(CF+SKM)으로 나누었다. SKM과 CF+SKM은 사람의 하루 섭취량을 기준으로 체중 ㎏당 7 ㎖씩 매일 일정시각에 경구투여 하였다. SKM과 CF+SKM은 체중과 식이섭취에는 영향을 미치지 않았으며, CF+SKM군의 신장무게가 대조군보다 낮았다. 혈장 중 에탄올 함량은 대조군에 비하여 CF+SKM군에서 유의적으로(p<0.05) 낮았으며, SKM군은 낮은 경향을 보였다. 혈장 중의 아세트알데히드 함량은 대조군에 비하여 SKM과 CF+SKM군 모두 각각 40.6%와 48.4% 유의적인(p<0.05) 개선 효과를 보였다. 간조직 중의 ADH 활성은 실험군간 유의적인 변화가 없었으나 CYP2E1 활성은 SKM과 CF+SKM 모두 대조군에 비하여 유의적으로 (p<0.05) 낮았다. 간조직의 CYP2E1 활성은 혈장 중의 아세트알데히드 함량과 양의 상관관계(r=0.566, p<0.01)였다. 간조직의 ALDH 활성은 SKM과 CF+SKM 모두 대조군에 비하여 유의적으로(p<0.05) 높았으며 혈장의 아세트알데히드 농도와 유의적 음의 상관관계(r=-0.564, p<0.01)를 보였다. SKM군과 CF+SKM군의 간조직내 SOD와 CAT 활성과 GSH 함량이 대조군에 비하여 유의적으로 높았다. 반면, SKM과 CF+SKM은 간조직 중의 지질과산화물 생성을 대조군에 비하여 각각 유의적으로 낮추었다. SKM과 CF+SKM 급여 시 에탄올대조군에 비하여 각각 AST 활성은 29%와 44% 낮았으며, ALT 활성은 42%와 34% 낮았다. 혈장의 총 콜레스테롤과 간조직의 콜레스테롤 함량은 대조군에 비하여 SKM과 CF+SKM군에서 유의적으로(p<0.05) 낮았으며 특히, CF+SKM의 간조직내 중성지질 함량은 대조군에 비하여 유의적으로(p<0.05) 낮았다. SKM군과 CF+SKM군의 간조직 중 지방축적이 대조군에 비하여 감소되었다. 이와 같이 SKM과 CF+SKM은 간조직의 CYP2E1 활성을 억제하고 ALDH 활성과 항산화 방어계를 향상시킴으로써 에탄올로 인한 간독성을 보호할 수 있을 것으로 사료된다. Cucumber fermentation has been used as a means of preservation. This study was performed to investigate the effects of fermented cucumber beverage (CF) containing beneficial materials for an ethanol hangover based on Hovenia dulcis (SKM) on ethanol-induced hepatotoxicity. Male Sprague-Dawley rats were randomly divided into three groups: ethanol control, ethanol plus SKM, and ethanol plus CF+SKM. SKM or CF+SKM was orally administered at a dose of 7 ㎖/㎏ body weight once per day for 5 weeks. Control rats were given an equal amount of water. CF+SKM significantly lowered plasma ethanol levels, whereas SKM tended to decrease the levels compared to the control. Both SKM and CF+SKM significantly lowered the plasma acetaldehyde levels and serum transaminase activities compared to those in the control. SKM and CF+SKM did not affect hepatic alcohol dehydrogenase activity; however, it significantly inhibited cytochrome P450 2E1 (CYP2E1) activity. Hepatic aldehyde dehydrogenase (ALDH) activity was significantly higher in the SKM and CF+SKM groups than that in the control group. Plasma acetaldehyde concentration was significantly correlated with hepatic CYP2E1 (r=0.566, p<0.01) activity and ALDH (r=-0.564, p<0.01) activity. Hepatic superoxide dismutase and catalase activities as well as glutathione content increased with the SKM and CF+SKM administration, whereas lipid peroxide content decreased significantly. Furthermore, SKM and CF+SKM lowered plasma and hepatic lipid content and lipid droplets compared to those in the control group. These results indicate that SKM and CF+SKM exhibit hepatoprotective properties partly by inhibiting CYP2E1 activity, enhancing ALDH activity and stimulating the antioxidant defense systems in ethanol-treated rats.

인체 전립선 암세포에서 재배 와송의 항암효과

원영선(Yeong Seon Won),이주혜(Ju Hye Lee),권순재(Soon Jae Kwon),안동욱(Dong Uk Ahn),신동영(Dong Young Shin),서권일(Kwon Il Seo) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.1

재배 와송과 천연 와송의 항암효과 및 apoptosis 유도효과를 입증하기 위하여 RC-58T/h/SA#4 primary 인체 전립선암세포를 이용하여 다양한 실험을 실시하였다. 그 결과 재배 와송과 천연 와송은 전립선암세포에서 농도 의존적으로 높은 암세포 증식 억제효과를 나타내었으며, 대조군과 비교하여 와송 메탄올 추출물 처리 시 세포의 형태가 수축되고 세포의 수가 감소한 것을 형태학적 관찰을 통해 확인할 수 있었다. 또한 와송 메탄올 추출물의 처리에 따른 RC-58T/h/SA#4 세포에서의 apoptosis 유도효과는 sub-G1기의 함량 증가와 hoechst 염색을 통한 apoptotic bodies 형성 및 핵 형태 변화를 관찰을 통해 확인할 수 있었다. 한편 재배 와송과 천연 와송은 dioxin과 bisphenol A와 같은 환경호르몬 처리에 의해 비정상적으로 증가된 전립선암세포의 성장을 억제하는 것으로 나타났다. 본 연구 결과, 재배 와송과 천연 와송은 RC-58T/h/SA#4 전립선암 세포에서 유사한 암세포 성장 억제효과와 apoptosis 유도효과를 가지고 있음을 확인 하였으며, 환경호르몬에 의해 유발될 수 있는 암에 대해서도 유사한 보호 효과를 가지고 있음을 증명하였다. This study was performed to determine the anticancer effects of cultivated Orostachys japonicus (COJ) and wild Orostachys japonicus (WOJ) on primary human prostate cancer cells (RC-58T/h/SA#4 cells). The morphology of cells treated with COJ and WOJ was distorted to shrunken cell masses. In addition, cell death induced by COJ and WOJ was associated with increased population of cells in sub-G1 phase as well as the formation of apoptotic bodies and nuclear condensation. COD and WOJ markedly reduced the number of viable prostate cancer cells in a dose-dependent manner, and cell numbers were lower than control cells. COJ and WOJ also inhibited increases in cell proliferation induced by environmental hormones such as dioxin and bisphenol A in charcoal-treated FBS (cFBS) medium. COJ and WOJ methanol extracts at the tested concentrations (150, 300, and 600 μg/mL) also dose-dependently inhibited cell proliferation induced by environmental hormones. These results indicate that COJ and WOJ exert anticancer effects on primary human prostate cancer cells.

임신성 당뇨 선별 및 진단검사로서의 1단계 검사법과 2단계 검사법에 대한 주산기 예후의 비교

박선화 ( Sunwha Park ),이주혜 ( Ju Hye Lee ),김보라 ( Bo Ra Kim ),박미혜 ( Mi Hye Park ),김영주 ( Young Ju Kim ) 한국모자보건학회 2021 한국모자보건학회지 Vol.25 No.1

Purpose: To compare pregnancy complications between the 2 groups of patients with gestational diabetes mellitus (GDM): those diagnosed by the 1-step method and those diagnosed by the 2-step method. Methods: In this retrospective cohort study, the data from outpatient and hospitalization medical records of 201 patients diagnosed with GDM between 2013 and 2017 were reviewed. We compared the pregnancy complications of these patients based on whether they were diagnosed by the 1-step or 2-step method. SPSS ver. 20.0 was used to analyze the data from the 2 groups. The odds ratio and 95% confidence interval of the pregnancy outcomes were estimated using binary logistic regression analysis. Results: On comparing pregnancy-related complications between the groups, there was no significant difference in the incidence of preeclampsia or delivery by cesarean section (p>0.99 and p=0.50, respectively). In the 1-step and 2-step groups, the prevalence of premature birth was significantly high at 19.7 % and 40.3% (p=0.01), respectively. There were no significant differences between the 2 groups in terms of macrosomia, large for gestational age (LGA), small for gestational age (SGA), low APGAR score, and neonatal hypoglycemia (p>0.99, p>0.26, p>0.62, p>0.57, and p>0.45, respectively). Conclusion: On comparing the 2 groups, we found that the 1-step and 2-step GDM groups had similar risks of pregnancy complications, namely preeclampsia, delivery by cesarean section, macrosomia, LGA, SGA, low APGAR scores, and neonatal hypoglycemia.

야콘 식초의 품질특성 및 항당뇨 효과

이미경(Mi-Kyung Lee),최사라(Sa-Ra Choi),이진(Jin Lee),최윤홍(Yun-Hong Choi),이주혜(Ju-Hye Lee),박경욱(Kyung-Uk Park),권승혁(Seung-Hyek Kwon),서권일(Kwon-Il Seo) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.1

본 연구에서는 2단계 발효를 통해 보당 없이 야콘 식초를 제조한 후 이에 대한 이화학적 성분 분석 및 항당뇨 효과를 조사하였다. 1단계 알코올 발효에서는 28℃에서 6일간 발효하여 알코올 함량 5.2%의 야콘 와인을 얻을 수 있었으며, 2단계 초산발효에서는 30℃, 200 rpm으로 6일간 발효하여 산도 4.75%의 야콘 식초를 생산할 수 있었다. 야콘 식초의 주요 유리당은 glucose와 fructose로 나타났으며, 유기산은 acetic acid가 가장 높았으며 이어서 succinic acid가 높게 나타났다. 야콘 식초의 유리아미노산 총 함량은 62.88 mg%로 proline, γ-amino-n-butyric acid, ornithine이 주된 아미노산으로 나타났다. 무기성분은 Ca, K, Mg와 같은 알칼리성 원소를 다량 함유하고 있는 것으로 나타났다. 제2형 당뇨병 마우스에게 야콘 식초를 4주간 경구투여 하여 혈당 강화 효과를 평가한 결과 공복 시 혈당은 5% 야콘 식초군에서 대조군에 비하여 유의적으로 낮았으며(p<0.05) 10% 야콘 착즙액과 10% 야콘 식초군은 낮은 경향이었다. 내당능 역시 5% 야콘 식초군이 대조군에 비하여 유의적으로 개선하였다(p<0.05). 혈장의 인슐린과 C-peptide 농도 및 췌장의 인슐린 농도는 실험군간 차이가 없었으나 췌장의 C-peptide 농도는 대조군에 비하여 야콘 식초에 농도 의존적으로 높은 것으로 나타났다. This study was performed to investigate the physiochemical properties and anti-diabetic effect of yacon vinegar by two-step fermentation. Yacon was matured at room temperature for 20 days. The sugar content of yacon juice prepared from mature yacon was approximately 14°Brix. In the first stage, yacon wine was produced from the juice at 28℃ for 6 days. In the second stage, acetic acid fermentation was conducted at 30℃ and 200 rpm for 6 days to produce yacon vinegar with 4.75% acidity. The major free sugars of yacon vinegar were glucose and fructose at 2,072.12 mg% and 463.95 mg%, respectively. The acetic acid content was the highest of the major organic acids at 3,881.44 mg%. The total free amino acid content was 62.88 mg% with the main free amino acids being proline, γ-amino-n-butyric acid and ornithine. The major minerals of yacon vinegar were Ca, K and Mg. The in vivo anti-diabetic activity of yacon vinegar was investigated in high-fat diet (HFD)/ streptozotocin (STZ)-induced diabetic mice. Diabetic mice were administered orally with 10% yacon juice and two yacon vinegars (5% and 10%) at a dose of 7 mL/kg body weight once per day for 4 weeks. Five% yacon vinegar improved the fasting blood glucose levels and glucose tolerance test significantly compared to the diabetic control group (p<0.05). Yacon vinegar increased the pancreatic C-peptide concentration in a dose-dependent manner. These results show that 5% yacon vinegar has a more potent effect on ameliorating hyperglycemia than 10% yacon juice.

Lightweight Design of a Sledge Frame for Para Ice Hockey Using Design for Additive Manufacturing

오은지(Eun-Ji Oh),이주혜(Ju-Hye Lee),김재은(Jae-Eun Kim),박근(Keun Park) Korean Society for Precision Engineering 2020 한국정밀공학회지 Vol.37 No.6

치주조직 재생유도를 위한 차폐막의 물성 및 세포친화성

이상배(Sang-Bae Lee),이주혜(Ju-Hye Lee),최성호(Seong-Ho Choi),김광만(Kwang-Mahn Kim),김경남(Kyoung-Nam Kim),이용근(Yong-Keun Lee) 대한치과재료학회 2007 대한치과재료학회지 Vol.34 No.4