Characterization and phylogenetic analysis of chitin synthase genes from the genera Sporobolomyces and Bensingtonia subrorea

남진식 한국환경생물학회 2005 환경생물 : 환경생물학회지 Vol.23 No.4

We cloned seven genes encoding chitin synthases (CHSs) by PCR amplification fromgenomic DNAs of four strains of the genus Sporobolomycesand of Bensingtonia subroseausingdegenerated primers based on conserved regions of the CHS genes. Though amino acid sequencesof these genes were shown similar as 176 to 189 amino acids except SgCHS2, DNA sequences weredifferent in size, which was due to various introns present in seven fragments. Alignment andphylogenetic analysis of their deduced amino acid sequences together with the reported CHSgenes of basidiomycetes separated the sequences into classes I, II and III. This analysis alsopermitted the classification of isolated CHSs; SgCHS1belongs to class I, bAelInd IcelIndIrelIvd INrelIn , and SsCHS1belong to class II, and BsCHS2belongs to class III. The deducedamino acid sequences involving in class II that were discovered from five strains were alsocompared with those of other basidiomycetes by CLUSTAL X program. The bootstrap analysisand phylogenetic tree by neighborjoining method revealed the taxonomic and evolutionaryposition for four strains of the genus Sporobolomycesand for Bensingtonia subroseawhich agreedwith the previous classification. The results clearly showed that CHS fragments could be used as avaluable key for the molecular taxonomic and phylogenetic studies of basidiomycetes.

Phenolic compounds in different parts of young Annona muricata cultivated in Korea and their antioxidant activity

남진식,박서연,장혜림,이영하 한국응용생명화학회 2017 Applied Biological Chemistry (Appl Biol Chem) Vol.60 No.5

The antioxidant activities of 80% methanol and distilled water extracts of different parts (roots, twigs, and leaves) of young Annona muricata were estimated based on their total phenol and flavonoid content as well as in vitro 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,20azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities, nitrite scavenging activity, Fe2?- chelating activity, ferric reducing antioxidant power (FRAP), and phenolic compound assays. The 80% methanol extracts showed a higher antioxidant effect than that of the water extracts. The 80% methanol root and leaf extracts showed higher total phenol (839.69 mg CAE/g) and flavonoid (168.52 mg RE/g) contents than those shown by the other extracts. In addition, the 80% methanol root extracts showed high DPPH (EC50 = 0.18 mg/mL) and ABTS (EC50 = 0.55 mg/mL) radical as well as nitrite (EC50 = 0.21 mg/mL) scavenging activities. The metalchelating effect of the 80% methanol twig extract was the highest, but there were no significant differences among the 80% methanol extracts of the different parts. FRAP values of all extracts increased in a concentration-dependent manner, except for those of the distilled water leaf extract, while the 80% methanol root extracts showed the highest value. In addition, there was a strong positive correlation between the antioxidant activity and total phenol content (P\0.01). A. muricata extracts were rich in various phytochemicals including rutin, epicatechin, ferulic acid, and p-coumaric acid. These findings indicate that A. muricata is a potentially useful source of substances with antioxidant effects.

Polymerase Chain Reaction에 의한 Lactobacillus casei 및 돌연변이 균주들의 비교 분석

남진식,이정준,신명수,나석환,백영진,유민 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.6

PCR 반응에 따른 RAPD를 산업적으로 유용한 유산균주를 신속하고 정확하게 분리하기 위한 방법의 하나로 사용할 수 있는지 가능성을 조사하기 위하여 L. casei의 4개 strain과 이들의 돌연변이 균주 2 종류를 비교하였다. 또한 L. casei로부터 농도와 순도면에서 PCR template에 적합한 chromosomal DNA를 3시간만에 추출할 수 있는 방법을 확립하였다. RAPD를 위하여는 degenerated primer를 이용하였는데 PCR 결과로 볼 때 분석에 적합한 수와 크기의 random product들이 증폭되었다. 본 실험에 사용된 조건과 primer들은 L. casei의 대상 균주들과 돌연변이균주들을 정확하고 특이적으로 구별할 수 있었는데 경우에 따라서는 적절히 primer들을 혼합하여 사용할 때 더욱 확실한 구별이 가능하였다. 본 실험의 결과는 반복성이 매우 우수한 것으로 확인됨으로서 장차 산업적으로 유용한 L. casei 균주들을 신속, 정확하게 분리, 동정하는 데에 이용 가치가 높을 것으로 사료된다. To classify Lactobacillus casei strains on the basis of difference in their chromosomal DNA sequence, we have performed polymerase chain reactions on their chromosomal DNA by using random primers, and followed by analyzing randomly amplified polymorphic DNA fragments. We also developed a mini-preparative method to isolate PCR-grade chromosomal DNA from Lactobacillus casei strains within 3 hours. Based on RAPD patterns by polymerase chain reactions with degenerated random primers, 4 Lactobacillus casei strains and 2 mutant strains were successfully discriminated. Results were very sensitive, strain-specific and reproducible. It was also reliable. These results suggest that RAPD may be applied efficiently for the identification of several Lactobacillus casei strains.

환경 및 Bioflocculant 생산균주의 분리 및 배양조건 검토

남진식,권기석,윤병대,이태호 한국미생물생명공학회 1992 한국미생물생명공학회 학술발표초록집 Vol.1992 No.2

베리류 열매 착즙액의 항산화 및 항균 활성

남진식,한영진,여수민,Nam, Jin-Sik,Han, Young-Jin,Yeo, Soo-Min 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.2

본 연구에서는 유기용매 추출물이 아닌 베리 열매 자체의 생리활성에 관한 정보와 천연보존료 및 건강기능식품 개발의 가능성을 모색하는데 기초자료를 제공하고자 다양한 종류의 베리 열매 착즙액의 항산화, 항균 작용 및 균 생육억제 효과를 비교 조사하였다. 총 폴리페놀은 체리, 아로니아 및 크랜베리가 다른 베리류에 비해 높게 함유되어 있었으며, 각 농도별로 측정한 베리 착즙액의 DPPH 라디칼 소거능은 모든 시료에서 농도에 따라 높아졌다. 특히, 아로니아, 크랜베리 및 라즈베리의 소거능이 다른 베리류에 비해 높은 것으로 확인되었다. FRAP 활성은 6가지 베리 열매 중 아로니아의 활성이 모든 농도에서 가장 높았으며, 100% 아로니아 착즙액의 FRAP 활성은 25% 포도 착즙액의 5배 이상이었다. 베리 열매 착즙액의 식중독 원인균에 대한 항균활성은 크랜베리와 라즈베리가 대부분의 식중독 균에 대해 활성을 나타내었으며, 대부분의 베리 열매 착즙액이 그람양성균보다 그람음성균에 대한 생육저해활성이 더 큰 것으로 확인되었다. 크랜베리와 라즈베리의 농도별 항균활성은 원액과 75%의 농도에서만 각각 7가지, 6가지 식중독 균에 대해 생육저해를 보였고, 50%, 25%의 농도에서는 식중독 균 중 일부만 생육저해를 나타내었으며, 10% 이하의 농도에서는 모든 식중독 원인균에 대한 항균활성을 보이지 않았다. 이상의 결과로 아로니아, 크랜베리 및 라즈베리는 다른 베리에 비해 높은 항산화 및 항균활성을 보임으로써 천연보존료 및 기능성식품 소재로써의 이용성이 높을 것으로 판단된다. This study was performed to evaluate and compare the antioxidant and antimicrobial activities of various berry juices (cherry, grape, blueberry, aronia, cranberry, and raspberry). The cherry, aronia, and cranberry juices included higher levels of total polyphenol than the other berry juices. The DPPH radical scavenging activity of various berry juices increased according to the juices concentration. The scavenging activity of DPPH radicals by the aronia, cranberry, and raspberry juices was higher than those of other samples and was in the following order: aronia > cranberry > raspberry. The FRAP values of 100% aronia juice was the highest among the samples, which was more than five times higher than grape juice at a concentration of 25%. The cranberry and raspberry juices exhibited a strong inhibitory effect against important food-borne bacteria, and in general, the berry juices inhibited the growth of Gram-negative bacteria more than that of Gram-positive bacteria. The cranberry and raspberry juices exhibited high antimicrobial activities against important food-borne bacteria at a concentration of 100% and 75%, however, they did not affect food-borne bacteria at a concentration below 10%. These results suggest that aronia, cranberry, and raspberry may be used effectively as natural additives and as functional foods due to their high antioxidant and antimicrobial activities.

수원여자대학교 식품분석연구센터

남진식 한국식품위생안전성학회 2012 Safe Food Vol.7 No.1

Nutritional compositions in young leaves and stem from quinoa (Chenopodium quinoa Willd.) grown in Korea

남진식,장혜림 한국식품저장유통학회 2022 한국식품저장유통학회지 Vol.29 No.6

Quinoa (Chenopodium quinoa Willd.) leaves and stem grown in Korea were analyzed to determine their nutritional compositions. Quinoa leaves exhibited significantly higher levels of crude protein, crude lipid, crude ash, carbohydrate, and dietary fiber than those of the quinoa stem. Fructose and glucose were only detected in stem, and they contents were 44.25 mg% and 107.62 mg%, respectively. Acetic acid was the most plentiful organic acid in leaves and stem of quinoa, with their higher levels determined in leaves (428.40 mg%). The major minerals of quinoa were potassium and calcium, especially potassium was verified as having the highest level in leaves (750.65 mg%) and stem (869.15 mg%) of quinoa. In addition, linolenic acid (C18:3, n-3) contained as highest level was detected only in leaves (176.09 mg%) but no identified in stem. It was found that quinoa leaves contained many kinds of vitamin such as vitamin B1 (293.35 μg/100 g), B2 (256.54 μg/100 g), C (2.02 mg/100 g), and E (2.25 mg/100 g). These findings suggest that quinoa leaves and stem are an excellent source for developing of salads and various processed products, further this study presents the application prospect of quinoa to various food industry fields.

아마인과 아마인유의 영양성분과 물리화학적 특성

남진식 ( Jin Sik Nam ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.4

Flaxseed has recently gained attention as a functional food. In this paper, physicochemical analyses of flaxseed and its oil were performed. Crude fat content ranged from 37~43%, moisture 0.2~6.8%, carbohydrate 30~35%, crude protein 18~23%, and crude ash 3~4%. Flaxseed is also an important source of dietary fiber. The TDF(total dietary fiber) contents of the flaxseed samples were 28~31%, and the SDF(souble dietary fiber) content of roasted flaxseeds was higher than that of raw flaxseeds. The major minerals found in flaxseed were calcium, potassium, magnesium, and phosphate. The flaxseeds were rich in γ-tocopherol with 234.3㎎/㎏ in raw brown flaxseed and 134.1㎎/㎏ in raw gold flaxseed, respectively. Roasted flaxseeds showed slightly lower vitamin and amino acid contents than those of the raw samples. The iodine, saponification, and acid values of brown flaxseed oil were 204.1g/100g, 193.6㎎/g, and 1.59㎎/g, and for gold flaxseed oil were 203.0g/100g, 189.9㎎/g, and 2.35㎎/g, respectively. α-Linolenic acid(ALA, C18: 3n-3) was highly concentrated in the flaxseed oil, which constituted about 55.5~56.1% of total fatty acids. Thus, flaxseed oil is a good source of omega-3 fatty acids and beneficial for the heart. Flaxseed contains high levels of dietary fiber including lignans, as well as minerals and vitamins, which may have antioxidant actions and help protect against certain cancers.

응집제 생산 미생물의 분리 및 배양조건

남진식(Jin-Sick Nam),권기석(Gi-Seok Kwon),황지숙(Ji-Sook Hwang),이문호(Mun-Ho Lee),장경립(Kyung-Lib Jang),윤병대(Byung-Dae Yoon),이태호(Tae-Ho Lee) 한국생물공학회 1995 KSBB Journal Vol.10 No.4

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2