항결핵제 투여후 중합효소연쇄반응으로 추적한 폐결핵 환자들의 치료반응 관찰

전창호 ( Chang Ho Jeon ),서헌석 ( Hun Suk Suh ),이상채 ( Sang Chae Lee ),현대성 ( Dae Sung Hyun ),안욱수 ( Wook Su Ahn ) 대한결핵 및 호흡기학회 1998 Tuberculosis and Respiratory Diseases Vol.45 No.5

다약제 내성 유전자를 이용한 항류마티스 약제의 내성 검사 방법 개발

김상경 ( Sang Gyung Kim ),서헌석 ( Hun Suk Suh ),최정윤 ( Jung Yoon Choe ),이종원 ( Jong Won Lee ),서장수 ( Jang Soo Suh ),김신규 ( Think You Kim ) 대한류마티스학회 2003 대한류마티스학회지 Vol.10 No.1

Objective: A number of disease-modifying anti-rheumatic drugs (DMARDs) have been shown to be more effective than placebo in the management of rheumatoid arthritis (RA). However, most course of DMARDs, except methotrexate, are discontinued after 2 or 3 years, because of toxicity, lack of efficacy or escape from control. The multi-drug resistance (MDR) is a phenomenon in which cells develop cross-resistance to many agents such as anthracyclin, vinca alkaloids and colchicine. In our hypothesis, MDR phenomenon could be implicated in acquired resistance to DMARDs in RA. We have established a mdr1 cell line and tested whether DMARDs are substrate for P-glycoprotein (P-gp). Methods: The mdr1-cDNA was cloned into retroviral vector, and the recombinant retroviral vector was transfected into PA317 cells. The target cells, NIH3T3, were infected with recombinant retroviruses. A colony most resistant to vinblastin was selected for the following experiments; expression of mdr1 gene in NIH3T3 cells was confirmed by RT-PCR, and biological function of mdr1 gene product, P-gp, was tested using Rhodamine-123 (Rh123) efflux assay. Resistance of the target cells expression P-gp which can survive against hydroxychloroquine (HCQ) and methotrxate (MTX) were measured by MTT assay. Results: RT-PCR for mdr1 gene showed successful transfer of the gene into the NIH3T3 cells. Rh123 assay revealed expression of P-gp on the selected cells as follows; Rh123 efflux activity of uninfected NIH3T3 cells was 6%, that of PLXSN was 0.2%, and that of selected cells was 44%. The 50% proliferation inhibitory capacity of the selected cells were twice for HCQ but there was no difference of that for MTX. Conclusion: We established a mdr1 cell line and using the cell line, HCQ was a substrate of MDR, but MTX was not related to MDR.

Retrovirus를 이용하여 조혈모세포에 유전자를 전달하기 위한 최적화

김상경(Sang-Gyung Kim),서헌석(Hun-Suk Suh),이종원(Jongwon Lee),신동건(Dong-Gun Shin),이재관(Jaegwan Lee),김현민(Hyun-Min Kim),김재식(jay-Sik Kim),서장수(Jang-Soo Suh) 한국생물공학회 1999 KSBB Journal Vol.14 No.5

적혈구 침강속도 자동측정기기안 TEST 1의 유용성 평가

박경희 ( Kyong Hee Park ),조주연 ( Ju Youn Cho ),조창호 ( Chang Ho Cho ),서헌석 ( Hun Suk Suh ) 대한임상검사과학회 2001 대한임상검사과학회지(KJCLS) Vol.33 No.2

Although the erythrocyte sedimentation rate (ESR) is a diagnostically nonspecific test, it is widely used for monitoring of inflammatory disorders. Conventional methods have disadvantages of difficu1ty in standardization and quality control. Thus we compared automatic TEST 1 system with the conventional Westergren method and Ves-Matic 60 for correlation with 205 patients. The results are summarized as follows l. The TEST 1 showed acceptable reproducibility(CV: <10.0 %). 2. The correlation between TEST 1 and conventional Westergren method showed good correlation(r=0.9 10, P<0.001). 3. The correlation between TEST 1 and Ves-Matic 60 showed good correlation(r=0.918, P<0.001). We conclude that TEST 1 is a suitable automated ESR analyzer that is accurate and easy to use for ESR

저산소 조건에서 항암제와 니트로이미다졸 복합제제가 인간 간암 세포의 생존에 미치는 영향

임선하(Sun Ha Lim),이준엽(June Yeob Lee),박성환(Sung Hwan Park),김여희(You Hee Kim),서헌석(Hun Suk Suh),박재복(Jae Bok Park),이종원(Jongwon Lee) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.76 No.6

Purpose: In a previous study, we have shown that anticancer agents inhibiting topoisomerases improve survival of tumor cells under hypoxic condition. In the present study, we evaluated whether and how cell survival effect of the anticancer agents under hypoxic conditions could be eliminated by the addition of nitroimidazoles, a class of bioreductive agents. Methods: Human hepatocellular carcinoma cells (HepG2) were incubated with different combinations of pimonidazole(1∼1,000μg/ml) and doxorubicin (0.1 or 1μg/ml) concentrations under different O₂ concentrations [1, 3, 5, 10 and 21 O₂]. Then cell numbers, glucose concentrations and lactic acid concentrations in the medium were measured, and DNA fragmentation assay was performed. Finally, different combinations of nitroimidazoles, such as pimonidazole, misonidazole, etanidazole, tinidazole, metronidazole, ornidazole or dimetridazole, and anticancer agents, such as doxorubicin, campothecin, epirubicin, dactinomycin, etoposide or mitomycin C was added to the cell culture medium under hypoxic conditions (1% O₂). Results: Pimonidazole at a concentration of 100μg/ml eliminated cell survival effect of doxorubicin at the concentrations of 0.1 and 1μg/ml under hypoxic condition (1% O₂) by promoting apoptosis. Almost all the cells died even after 24 hours of incubation for all the oxygen concentrations at a combination of 100μg/ml pimonidazole and 1μg/ml doxorubicin. Finally, pimonidazole at a concentration of 100μg/ml, and misonidazole or etanidazole at a concentration of 1,000μg/ml eliminated cell survival effect of all the anticancer agents tested under hypoxic condition. Conclusion: Combination therapy of doxorubicin (adriamycin) with pimonidazole can maximize dororubicin efficacy by eliminating cell survival effect of doxorubicin under hypoxic conditions in treating solid tumors, such as breast cancer.

저산소 조건에서 항생제들이 인간 간암 세포의 생존에 미치는 영향

이영택(Young Tag Lee),한미정(Mee-Jung Han),임선하(Sun Ha Lim),박성환(Sung Hwan Park),서헌석(Hun Suk Suh),박재복(Jae Bok Park),김양일(Yang Il Kim),이종원(Jongwon Lee) 대한외과학회 2006 Annals of Surgical Treatment and Research(ASRT) Vol.71 No.1

저산소조건에서 항암제들이 인간간암세포주의 생존에 미치는 영향

이준엽 ( June Yeob Lee ),임선하 ( Sun Ha Lim ),박성환 ( Sung Hwan Park ),안기성 ( Ki Sung Ahn ),서헌석 ( Hun Suk Suh ),이종원 ( Jongwon Lee ) 대한내과학회 2007 대한내과학회지 Vol.72 No.4

목적: 선행연구에서 위상이성질화효소 저해제로 작용하는 퀴놀론 항생제들이 저산소조건에서 암세포의 생존을 개선시키는 것을 밝혔다. 본 연구에서는 위상이성질화효소 저해제로 작용하는 항암제들도 또한 저산소조건에서 암세포의 생존을 개선시키는지 조사하였다. 방법: 인간간암세포인 HepG2를 60 mm 배양 접시에 2.5×10(5)개의 세포농도로 4 mL의 배양배지로 하여 정상산소조건에서 2일 동안 키운 다음 독소루비신이나 다른 항암제들이 들어있는 새로운 배지로 교환하여 정상산소 또는 저산소 조건(1% 산소분압)에서 키웠다. 배양시간에 따라 생존한 세포수, 배지 내에서의 포도당과 젖산농도가 측정되었다. 동시에 60 mm 배양배지에 들어있는 세포들을 파괴하여 염색체 DNA를 얻은 다음 DNA 분절분석을 위해 1.5% 에가로즈 젤에 전기영동을 실시하였다. 결과: 정상산소 조건 하에서 독소루비신은 0~100 μg/mL 농도범위에서 농도의존적으로 세포의 성장을 억제하였다. 하지만 동일한 독소루비신이 저산소 조건하에서는 0.1~10 μg/mL 농도범위에서 오히려 세포자살을 억제함으로써 세포 생존을 증가시켰다. 이러한 현상들은 위상이성질화효소 저해제로 작용하는 다른 항암제들에 대해서도 관찰되었다. 결론: 고형암은 그 암조직 내에 저산소 지역이 존재하므로, 위상이성질화효소 저해제로 작용하는 항암제들은 암세포의 사멸을 지연시킬 수 있다. 이에 따라 항암제의 효과가 낮아질 수 있다. Background: In a previous study, we have shown that quinolones, antibiotics inhibiting topoisomerases, improve survival of tumor cells under hypoxic conditions. In this study, we tested whether antitumor agents such as doxorubicin that inhibit topoisomerases can also improve survival of tumor cells under hypoxic conditions. Methods: Human hepatocellular carcinoma cells (HepG2) were grown in 4 mL of the culture medium at 2.5×10(5) cells/60 mm culture dish under normoxic conditions for 2 days before being transferred to fresh culture medium with different concentrations of doxorubicin or other antitumor agents under normoxic or hypoxic (1% oxygen concentration in air) conditions. Cell viability and the concentration of glucose and lactic acid in the medium were measured during cell culture. At the same time, the cells in the 60 mm dishes were lysed, and chromosomal DNA was isolated and loaded onto a 1.5% agarose gel for the DNA fragmentation assay. Results: Doxorubicin inhibited cell growth under normoxic condition in a concentration-dependent manner for the 0~100 μg/mL concentration range. However, doxorubicin improved cell viability under hypoxic conditions for a 0.1~10 μg/mL concentration range by inhibiting apoptosis. Similar phenomena were observed for other antitumor agents that inhibit topoisomerases. Conclusions: Solid tumors usually have hypoxic regions in the tumor, under which conditions antitumor agents that inhibit topoisomerases may function to delay tumor cell death. This can reduce the efficacy of the antitumor agents. (Korean J Med 72:384-392, 2007)

사람골수줄기세포가 연골조직으로 분화되는 과정에 나타나는 세포표지자의 표현

김상경 ( Sang Gyung Kim ),최정윤 ( Jung Yoon Choe ),김채기 ( Chae Gi Kim ),정승혜 ( Seung Hie Chung ),신임희 ( Im Hee Shin ),서헌석 ( Hun Suk Suh ) 대한류마티스학회 2005 대한류마티스학회지 Vol.12 No.1

Objective: Multipotent bone marrow stromal cells have the ability to differentiate toward a variety of connective tissue lineages including cartilage. The future use of adult mesenchymal stem cells (MSCs) for human therapies depends on the establishment of preclinical studies. Therefore, in this preclinical study we demonstrated the expression of MSC surface markers CD29, CD105, and CD44 on human bone marrow derived stromal cells during chondrogenic differentiation. Methods: Adult human bone marrow was collected from the iliac crest of 7 donors following informed consent. Mononuclear cells were isolated, incubated in monolayers, and embedded in alginate beads for three-dimensional cultures. Cellualr viability was assessed by MTT assay. Flow cytometry of alginate bead cultures was performed on days 0, 7, 14, 21, and 28 using monoclonal antibody against surface molecules, CD105, CD29, CD44, CD34 and CD45. Total contents of collagen and glycosaminoglycan (GAG) of the alginate beads was measured. SPSS 11.0 was used for data analysis. Results: After 7 days of culture, 89% of the cells expressed the human integrin beta 1 antibody, CD29. The CD29-positive cells remained elevated at 83% on days 28. However, while only 18% expressed the type II TGF-beta receptor endoglin, CD105 on day 7, the CD105-positive cells increased abruptly 65% on day 14 remaining elevated up to day 28. The expression of CD44 was maximal in the first passage cell (63%). High concentration of TGF-beta 3 (10 ng/mL) was more favorable for sustaining cell viability than a low concentration (0.5 ng/mL)(n=4, p=0.002, day 21). The total contents of collagen and GAG in the MSC-alginate beads increased during the three-dimensional culture (n=4, p=0.02, p=0.006) suggesting its differentiation into a chondrogenic lineage. Conclusion: CD29 was expressed earlier than CD105 during chondrogenic differentiation of human bone marrow MSC. CD44 expression was highest in the first passage cells and gradually decreased afterwards.

頸部腫瘤에 關한 臨床病理學的 檢索

金春元,徐憲錫 한양대학교 의과대학 1982 한양의대 학술지 Vol.2 No.1

lumps of the neck are easily found by patient and physicians due to inspection and palpation, Tuberculosis, nonspecific inflammation and metastatic lesions are commonest. Abundance of lymph nodes in the neck and centropedal drainage of the body lymphatics into the neck region makes the neck favorite metastatic site of the neoplastic lesions of the facimaxilla, thorax and systemic. Durinh the past 8 years and 7 months from May 1972 to Dec. 1980, 517 cases of neck mass confirmed by pathological examination. The results were as follows. 1. Histopathologically congenital lesions were 6.2%, inflammatoty lesions 51.5%, benign tumor 10.2%, primary malignant tumor 8.1%, metastatic malignanl tumor 21.5% and others 2.3%. Most common lesion is tuberculous lymphadenitis which is 36.8%. Primary lesions of metastatic malignant tumor show 27% of lung and bronchogenic 12.6% of stomach, 6.3% of liver. 2. Classification of lymphoma shows 71.4% of Non-Hodgkin's lymphoma, 16.7% of Hodgkin's disease adn 11.9% of reticulum cell sarcoma. In Non-Hodgkin's lymphoma, nodular type were 47.6% which most common type is mixed cell type as 26.2% and diffuse type is 16.7%. 3. In sex incidence, male was 48.5% and female was 51.5%. In age incidence, 21-30 years old age group was 24% which is most common. Congenital lesion, primary malignant tumor and metastatic tumor show higher incidence in male than female as 2.2:1, 3.8:1 and 1.7:1, respectively. Inflammatory lesion shows higher incidence in female especially on tuberculous lymphadenitis. 4. Regarding the site of lesion, right side was 34.4% of total case left side was 36.6%, bilateral was 10.6%, anterior portion was 3.1%, posterior portion was 3.2% Nineteen percent of lymphoma was edveloped bilaterally. Metastatic carcinoma was developed at supraclavicular area, 79.3%. 5. Forty-five percent of total case developed within 1 year. 6. The rumor size measures up to 1 Cmw as 25.7%, within 1-3Cm was 38.3%. 7. Most common symptom and sign was palpable mass in 60%. In metastatic carcinoma, respiratory and G-I symptom were 43%. In tuberculous lymphadenitis, fever and chill with respiratory symptoms were 27.8% and the positive lesion in chest X-ray was 29.6% of 71 checked case, and 63.4% was normal findings.

Ceroid 조직구증 1예

서헌석,정용호,정화순,최일영,기춘석,김춘원,박승함,김기홍 대한병리학회 1982 Journal of Pathology and Translational Medicine Vol.16 No.3