Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

췌십이지장절제술 후 발생한 지속적인 구토증과 Wernicke 뇌병증

박우영,김성완,이삼연,신일선,김재민,박기형,양수진,윤진상 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.3

Wernicke encephalopathy is an acute neurologic disorder attributable to thiamine (vitamin B1) deficiency. We report the case of a 61-year-old female patient who presented Wernicke encephalopathy after surgery for pancreatic head cancer. From the ninth postoperative day, she had suffered from nausea and vomiting and had difficulties ingesting food, she was given total parenteral nutrition (TPN), but lacked adequate vitamin (thiamine) supplementation. After 28 days, she developed ataxia, ophthalmoplegia, and mental confusion. The magnetic resonance image showed pathologic changes in the medial thalamus,periaqueductal gray matter, medulla and mamillary bodies. The blood level of thiamine was very low. After intravenous and oral supplementation of thiamine (200 mg/day), consciousness was soon normalized and neurologic symptoms have gradually been improving. Nausea and vomiting disappeared after administration of a low dose of mirtazapine (7.5 mg/day). We emphasize the importance of thiamine supplementation to the patients who suffer from vomiting which hinders them from taking food and who require prolonged TPN.

초음파 에코파형의 웨이브렛 변환과 비파괴평가에의 응용

박익근,박은수,안형근,권숙인,변재원 한국비파괴검사학회 2000 한국비파괴검사학회지 Vol.20 No.6

초음파 에코파형의 시간-주파수해석법으로 Wigner 분포와 웨이브렛 변환 등과 같은 새로운 신호처리 기법이 비파괴평가 분야에 널리 응용되고 있다. 본 연구에서는 웨이브렛 변환(Wavelet transform)에 의한 음속과 감쇠계수의 주파수의존성과 초음파 결함신호의 잡음제거의 유용성 유무를 실험적으로 검증하였다. Gabor 함수를 웨이브렛의 기본 함수로 사용하였다. 초음파의 에코파형에 포함된 각 주파수성분의 속도와 감쇠계수의 주파수의존성을 추정할 수 있었으며, 초음파탐상에서 결함의 검출능 향상과 결함크기 산정의 정량화에 접근하기 위해 웨이브렛 변환에 의한 S/N 비신호처리 시뮬레이션 결과를 오스테나이트강 스텐인레스 용접부에 가공한 EDM 노치의 초음파 결함신호에 적용한 결과 임상에코를 저감하고 S/N 비를 개선하는 것이 가능하였다. Recently, advanced signal analysis which is called "time-frequency analysis" has been used widely in nondestructive evaluation appications. Wavelet transform(WT) and Wigner Distribution are the most advanced techniques for processing signals with time-varying spectra. Wavelet analysis method is an attractive technique for evaluation of material characterization nondestructively. Wavelet transform is applied to the time-frequency analysis of ultrasonic echo waveform obtained by an ultrasonic pulse-echo technique, In this study, the feasibility of noise suppression of ultrasonic flaw signal and frequency-dependent ultrasonic group velocity and attenuation coefficient using wavelet analysis of ultrasonic echo waveform have been verified experimentally. The Gabor function is adopted the analyzing wavelet. The wavelet analysis show that the variations of ultrasonic group velocity and attenuation coefficient due to the change of material characterization can be evaluated at each frequency. Furthermore, to assure the enhancement of detectability and flaw sizing performance, both computer simulated results and experimental measurements using wavelet signal processing are used to demonstrate the effectibeness of the noise suppression of ultrasonic flaw signal obtained from austenitic stainless steel weld including EDM notch.

조갑진균증에서 Ciclopirox Nail Lacquer의 치료효과

박경찬,김정애,방형돈,조광현,윤재일,김병수 대한감염학회 1995 감염 Vol.27 No.3

배경 및 목적:조갑진균증의 치료에 있어 국소용 항진균제는 조갑판을 통과하지 못하기 때문에 치료율이 낮은 것으로 알려졌으나 최근 조갑판을 통과하여 조상에서도 높은 치료농도를 유지할 수 있는 국소용 항진균제인 ciclopirox nail lacquer가 개발되었다. 이에 본 저자들은 8% ciclopirox nail lacquer 단독 도포의 조갑진균증에서의 치료 효과와 안전성을 알아 보기 위한 연구를 수행하였다. 방법:표적 조갑판의 감염된 면적의 비율이 50%이하인 경증의 조갑진균증 환자 27명을 평가 대상으로 하였으며 8% ciclopirox nail lacquer 1일 1회 도포요법으로 6개월간 치료하였다. 매 방문시마다 감염면적 비율을 기록하였고 치료 전과 치료 후 진균 검사를 시행하였다. 결과: 1)표적조갑판의 감염 면적이 치료 전 평균 28.9%에서 치료 종료시 4.7%로 통계적으로 유의하게 감소하였다(p<0.001). 2)KOH 직접 도말 검사와 진균 배양 검사에서 모두 음성인 진균학적 완치율은 치료 종료시 77.8%였다. 3)부작용으로는 3명(11.1%)에서 일시적 작열감, 조갑 감입 등을 호소하였다. 결론:경증의 조갑진균증 치료에 있어 8% ciclopirox nail lacquer는 비교적 좋은 치료 효과를 보이며 부작용이 적은 약제로 생각된다. Background: Topical antifungal agents have been ineffective in the treatment of onychomycosis because they could not reach the infected nail bed. 8% ciclopirox nail lacquer is a new topical antimycotic which is characterized by excellent penetration into the nail plate. We performed a study to assess the therapeutic efficacy and safety of 8% ciclopirox nail lacquer in the treatment of onychomycosis. Methods: We assessed 27 onychomycotic patients whose affected area of target nail plate was not more than 50%. The patients were instructed to apply 8% ciclopirox nail lacquer once daily and evaluated monthly for 6 months. Mycological examinations were done before and after the treatment. Overall efficacy, tolerability, cosmetic acceptance and adverse effects were assessed at the end of 6 months' treatment. Results: The mean percentage of affected area decreased from 28.9% to 4.7%. Mycological cure rate was 77.8% at the end of the treatment. Overall efficacy assessed by physician and patients were similar and evaluated as complete cure in 48% and 44%, respectively. Tolerability and cosmetic acceptance were excellent or good in most of the patients. No systemic adverse effects were found, but 3 patients complained of mild prickling sensation or ingrowing toe nail. Conclusions: These data suggest that 8% ciclopirox nail lacquer is safe and effective in the treatment of mild onychomycosis.

전류제어에 의한 단상유도전동기의 기동특성 개선

박수강,최낙일,백형래,임양수,이기용,신사현 조선대학교 에너지.자원신기술연구소 1998 에너지·자원신기술연구소 논문지 Vol.20 No.1

A single phase induction motor(SPIM) with two windings, main and auxiliary, is probably the most widely used motor in the world due to their ruggedness, low maintenance and simplicity of construction There are several ways of starting single phase motors The most popular arrangements is centrifugal switch An auxiliary winding connected in series with a capacitor, is brought into the circuit during starting The closing and opening of the switch must be speed dependant The switch meets the speed requirements, but it also has many drawback random switching, arcing, many models necessary for different speeds and shaft diameters, space required inside the motors and limited lifetime to name some of them. In this paper, the starting current of single phase induction motor was detected by a small detector and determined TRIAC gate signal. The strategy of current control is based on limited main and auxiliary current angle during starting period in the closed-loop control. The experimental result show the motor's starting characteristics of the proposal starting system and are compared with centrifugal switch starting motor.

前腕部 經穴 取穴에서 骨度分寸法과 一夫法의 比較 硏究

박히준,채윤병,차웅석,박종배,이혜정,이향숙,인창식,고형균,김수영,최일환,김강식,문정배,배기태,유경환,육근영,정병주,손인철,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Background : The cun measurement System, an essential and convenient method in locating acupoints, has been widely used in the practice of acupuncture. However, traditional cun measurement has been criticized for its lack of reliability. Objectives : The purposes of this study are to determine if one cun measured by the directional methods have a consistency with that of proportional methods and to investigate which factors are related with these differences, especially in forearm. Methods : The distance between the elbow crease and the wrist crease of forearm was compared to a reference value of one cun obtained by the directional method. In this method, one cun is one third of the distance between index finger and Small finger of a subject, measured at proximal interphalangeal joint. In addition, to investigate the factors influencing the differences between these two methods, we measured the height and body weight and calculated body mass index (BMI). Finally we analyzed the factors correlated with these lengths by linear regression test. Results : The results showed that one cun obtained by the directional methods were significantly different from one cun by the proportional methods in forearm. It was demonstrated that the length acquired with the directional method was more correlated with body weight and body mass index, while the length obtained by the proportional method was more correlated with the height.

단상유도전동기의 최적 구동을 위한 디지털 위상각 제어기 설계

박수강,백형래,김평호 조선대학교 에너지.자원신기술연구소 1999 에너지·자원신기술연구소 논문지 Vol.21 No.2

The availability of microcontrollers and switching power devices such as power MOSFET, IGBT, TRIAC, ect., offer improvement opportunities in the field of ac drive system control. In this paper, a microprocessor-based controller for self-starting and power saving single phase induction motor driven system from a variable phase angle power supply is described. The controller is capable of operating in the maximum efficiency region as well as in the optimal input power region. The implementation of the software is based on an PIC16C71 8-bit programmable microcontroller that has the potential of computing the switching instants on line an in real-time. The variable phase angle switching strategy more employed for self-starting and phase angle control of the motor than the PWM switching strategy. The performance of the developed system is evaluated using computer simulation on a 210W capacitor starting motor. Finally the validity and performance capability of the controller are experimentally demonstrated on a 210W single phase induction motor.

Carbofuran 土壤混入處理의 水稻害蟲 防除效果 및 水稻生育에 미치는 影響

朴亨萬,朴重秀,崔承允,李炯來 서울大學校 農科大學 1981 서울대농학연구지 Vol.6 No.1

In 1980, a field experiment was conducted to evaluate the effects of soil-incorporated Carbofuran (Furadan 3G) on some rice insect pests and on the growth of rice plants of the variety Milyang-23, which is one of the Korean leading varieties. The effects were compared with those of the conventional broadcast of Carbofuran and Diazinon as local standard method. The results obtained were as follows: (1) The soil incorporation of Carbofuran dramatically suppressed the plant damages of the rice stem maggots (Chlorops oryzae and Hyderellia sasakii) and thrips (Baliothips biformis) at tillering stage of the rice; plant damages observed were 0.5%(1.2 a.i. kg/ha) and 0.6%(1.8 a.i. kg/ha) for the rice stem maggots, and 1.3%(1.2 a.i. kg/ha) and no damage(1.8 a.i. kg/ha) for thrips while with broadcast of Carbofuran there were 65.9%(1.2 a.i. kg/ha) and 42.4%(1.8% a.i. kg/ha) for ricestem maggots, and for the thrips, 60.6%, 36.0% with Carvofuran baoadcast, and 83.5%, 90.7% with Diazinon treatment, and 97.8% for the untreated check.(2) Soil incorporation and broadcast of Carbofuran were significantly effective for the control of brown planthoppers (Nilaparvata lugens) and the white-backed planthoppers (Sogatella furcifera), and the conventional broadcast of Diazinon was still unsatisfactory for controlling the brown planthoppers. (3) on 38th day after transplanting, the number of tillers per hill and plant height in the soil incoporation of Carboruran revealed the significant increases over those of the Carbofuran and Diazinon brodcasts and the check; with number of tillers per hill 23.0 for 1.2 a.i. kg/ha and 25.7 for 1.8 a.i. kg/ha with number of tillers per hill 23.0 for 1.2 a.i. kg/ha and 25.7 for 1.8 a.i. kg/ha with Carbofuran soil incorporation, while they were for 20.1, 22.1, with respective doses of the broadcasted Carbofuran they were 19.1 for the broadcased Diazinon, and 18.5 for the check, on the 82nd day after transplanting, root development of rice plants grown on the Carbofuran soil incorporated sites also revealed a significant increase over the broadcasting treatments and the check in the number and length of roots.(4) Even under the unusual low-temperature conditions during 1980 in Korea, Carvofuran soil incorporation greatly promoted the heading of rice plants and consequently resulted in significant shortening of the heading time as compared to those of Carbofuran and Diazinon broadasts and the check. In addition, soil incorporation of Cabofuran resulted in much better heading status(65 to 70% in completed heading) than broadcast(35 to 37% in Carbofuran broadcast and 20% in Diazinon broadcast) and the untereated check(30%). (5) The mexmium growth promotion of rice plants was observed from the rate of 1.8 a.i. kg/ha of the Carbofuran soil incorporation in plant height (90.0cm), stem height(78.6cm), length of uppermost internodes(24.7cm), and root weight(5.0g/hill in dry base) over the untreated check(81.9cm in plant height, 62.9cm in stem height, 19.3cm in uppermost intermodes and 3.6g in root weight) and other insecticide treated plots. However, there was no singnificant difference in panicle length among the treatments. (6) The number of panicles per hill, number of grains per panicle and 1000-grain weight showed that Carbofural soil incorporation significantly increased those yield components as compared to the broadcast and the untreated check. Of the treatments, Carbofuran soin incorporation registered significantly higher grain yields (2,460kg/ha from 1.2 a.i. kg/ha and 2,244kg/ha from 1.8 a.i. kg/ha treatments) than Carbofuran broadcest (1,005kg/ha from 1.2 a.i. kg/ha and 1,185kg/ha from 1.8 a.i. kg/ha treatments), Diazinon broadcast(63kg/ha), and the check(849kg/ha). Showing the low yield potential of the test variety seemed to be cold-damage from unusual weather conditions in 1980. (7) Analysis of essential macro-and micro-element contents in rice plants on the 122nd day after transplanting showed more k, Cu, Mn and Zn contents in the plants treated by soil incorporation of Carbofuran than those in the untreated check, while N and Fe were lower in the plants treated by Carbofuran than those in the untreated check, while N and Fe were lower in the plants treated by Carbofuran soil incorporation. Amounts of K and Zn were much greater in Carbofuran soil incorporation than in Carbofuran broadcast, and N and P were greatly lower in the plants by Carbofuran soil incorporation. (8) in conclusion, the result obtained in this study would provide some real evidences that Carbofuran incoporated with the paddy soils could be a new insecticidal application method of the maximization of rice yield through the dramatical suppressin of the plant damages of insect pests; the rice leaf stem maggots, thrips, and planthoppers, and great growth stimulation(increasing the root development, the effective tillers, panicles, etc.) of rice plants.

[논문]병렬 유전 알고리즘을 이용한 발전기 기동정지계획

김형수,문경준,박준호 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

본 논문에서는 병렬 유전 알고리즘을 이용한 발전기 기동정지계획을 제안한다. 최소 기동 및 정지 시간 등과 같은 다양한 발전기의 제약조건을 만족시키면서 발전기 기동정지계획을 수립하는 문제는 비선형적이며 많은 국부해가 존재하므로 최적해를 탐색하는데 많은 시간이 소요된다. 이러한 문제점을 해결하기 위해 16개 의 프로세서를 가진 병렬 시스템과 이를 이용한 병렬 유전 알고리즘을 제안하였으며, 제안한 방법의 효용성을 검토하기위해 10기 및 26기의 전력계통에 적용하여 시율레이션을 시행하였다. 시율레이션 결과 기존의 방법에 비해 탐색속도를 개선하였고 우수한 해를 구할 수 있었다.

내시경적 조임근절개술후에 발생한 췌십이지장 가성동맥류 1예

박수영,김윤정,박진한,전현수,박시형,이영진,이은지,김지현 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

We reported a case of pancreatic pseudoaneurysm, involving pancreaticoduodenal artery, developed after endoscopic retrograde cholangiopancreatography(ERCP) with endoscopic sphinterotomy. A 54 year old woman was transferred to our hospital due to sustained hematochezia and hypovolemia. A week before admission, she underwent ERCP with endoscopic sphinterotomy for acute cholangitis in local clinics. She developed abdominal pain and hematochezia at 12 hours after the procedures. A post procedure contrast enhanced computed tomography of abdomen demonstrated a pseudoaneurysm in inferior portion of pancreatic head. This was not present on the pre procedure contrast enhanced computed tomography of abdomen and was thought to arise from the pancreaticoduodenal artery as a complication of the pre sphincterotomy. She was brought to our emergency room in a state of hypovolemia. A upper endoscopy showed active bleeding from ampula of vater. An celiac angiography demonstrated pancreatic pseudoaneurysm involving inferior pancreaticoduodenal artery and selective coil embolization of was performed.