증폭(蒸曝)에 의한 지황(地黃)의 성분 변화에 대한 고찰

정재우 ( Jae Woo Jung ),김한영 ( Han Young Kim ),류지효 ( Ji Hyo Lyu ),김정훈 ( Jung-hoon Kim ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.6

Objectives : ‘Steaming and drying’ is a traditional processing method that has been used to produce Suk-ji-hwang (熟地黃; Rehmanniae Radix Preparata) from Ji-hwang (地黃, the fresh root of Rehmannia glutinosa Liboschitz ex Steudel; Rehmanniae Radix). The steaming and drying process, which is proceeded in heating and moisturizing conditions, plays a crucial role in the change of therapeutic effect of Ji-hwang, presumably due to the modification of its chemical constituents. In this article, the chemical influence of the ‘Steaming and drying’ process was investigated for understanding the underlying mechanism of chemical modification of Ji-hwang. Methods : The articles regarding the modifications of chemical constituents of Ji-hwang during the ‘Steaming and drying’ process were collected and analyzed to investigate the influence of the processing to Ji-hwang. Results : The results indicated that iridoid glycosides were degraded to their aglycones and sugars, and such degradations occurred faster at a high pressure than at an atmospheric pressure during the process. The contents of catalpol, ajugol, and acteoside were decreased, while those of rehmannioside A and D were slightly increased during the repeated processing. The contents of oligosaccharides, namely sucrose, maltose, raffinose, and stachyose (except for manninotriose), were decreased, while those of monosaccharides, glucose and fructose, were increased by the repeated processing. Conclusions : These results demonstrate that the ‘Steaming and drying’ process influenced the chemical constituents of Ji-hwang and provide probable basis for the therapeutic modification of Suk-ji-hwang after the processing of Ji-hwang.

Aptamer‑linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT)

Woo‑Ri Shin,Mun‑Jong Lee,SEKHONSIMRANJEET SINGH,Ji Hun Kim,Sun Chang Kim,Byung‑Kwan Cho,Ji‑Young Ahn,Yang‑Hoon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E. coli. The high-affinity PAT-specific single-stranded DNA (ssDNA) aptamers were obtained from a random DNA library. MOE docking study was performed to identify the potential binding region of the selected aptamers on PAT. Aptamer-linked immobilized sorbent assay (ALISA) method was used to detect PAT. Conclusion We screened aptamer against PAT for developing an efficient detection method. The selected PAT specific aptamers, HRPA-05 and HRPA-07, showed the distinct target binding behaviors, and detected PAT protein by aptamer-linked immobilized sorbent assay method with high efficiency and selectivity.

영골(靈骨).강압혈(降壓穴) 자극(刺鍼)이 혈압(血壓)에 미치는 영향(影向)

임영남,심성용,한지완,고호연,박종형,한양희,전찬용,김동우,Yim, Young-Nam,Sim, Sung-Yong,Han, Ji-Wan,Ko, Ho-Yeon,Park, Chong-Hyeong,Han, Yang-Hee,Chun, Chan-Yong,Kim, Dong-Woo 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.2

Objective : The purpose of this study is to evaluate the clinical effect of the non-pharmacological anti-hypertension method, acupuncture, for hypertensive patients. Methods : 26 patients diagnosed with hypertension were each treated by one of three methods: an anti-hypertension drug(adalat soft capsule), venepuncture, and acupuncture. In cases of rising blood pressure, patients were treated by their one of the three methods, and blood pressure was measured at regular intervals. Result : As for the group of acupuncture treated patients on Young-gol(ling-gu) and Gang-ap-hyul(jiang-ya-xue), readings dropped 18.0mmHg in systolic blood pressure and 6.50mmHg in diastolic blood pressure. This was a greater effect than that seen in the venepuncture treated patients. Blood pressure checked one hour after acupuncture treatment showed a greater difference than blood pressure checked after thirty minutes. Conclusion : Safe reductions in blood pressure were observed through the non-pharmacological method of acupuncture on Young-gol(ling-gu) and Gang-ap-hyul(Jiang-ya-xue).

Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

고령자 남녀와 20대 남녀에 있어서 다양한 등속수축중 최대 모멘트의 비교

金智源(Ji-Won Kim),金相佑(Sang-Woo Kim),文基旭(Ki-Wook Moon),嚴光文(Gwang-Moon Eom),李泳揆(Young-Gyu Lee),南貞淑(Jung-Sook Nam),朴寬龍(Kwan-Yong Park),金耀翰(Yo-Han Kim),洪廷和(Jeong-Hwa Hong),朴炳奎(Bung-Kyu Park) 대한전기학회 2008 전기학회논문지 Vol.57 No.10

In this paper, we investigated the maximum ankle joint plantarflexion moment (MPM) of young and elderly subjects during various modes of isokinetic contractions with special interest in the elderly females who were reported to have higher fall rate than the elderly males. Subjects include the young subjects (14 males: 22.7±2.5yrs, 13 females: 20.5±1.3yrs) and the elderly subjects (17 males: 65.8±10.5yrs, 26 females: 71.4±5.2yrs). The MPM was normalized by each subject's body weight. The MPM of elderly females was the weakest among the groups, particularly, at the eccentric contractions. The male-to-female ratio of MPM was greater in the elderly than in the young and the young-to-elderly ratio of MPM was greater in female than in male, both during the eccentric contractions. The result suggests that the decreased muscle force per body weight, especially at the eccentric contraction, may be one reason for the more frequent fall of the elderly female than the elderly male.

심한 골병변으로 발현된 기능성 낭종성 부갑상선 선종

전숙,김영희,박지영,고관표,박철영,김덕윤,우정택,김성운,김진우,김영설,고석환 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

낭종성 부갑상선 선종과 심한 골병변을 동반한 부갑상선 기능항진증은 매우 드문 질환으로서, 저자들은 양측 고관절의 통증을 초기 주소로 내원한 환자에서 고칼슘혈증과 부갑상선 호르몬 증가, 골병변의 방사선적 소견을 통해 부갑상선 기능항진증을 진단하고, 경부 초음파와 컴퓨터 단층 촬영, 부갑상선 스캔검사 및 수술중 부갑상선 낭종액 검사 등을 통해 기능성 부갑상선 낭종의 한 종류인 낭종성 부갑상선 선종을 진단하고 수술적 제거를 통하여 정상화된 1예를 경험하였다. A cystic parathyroid adenoma is rare. A case of primary hyperparathyroidism, with the cystic formation of a parathyroid adenoma and a severe bony lesion, is reported. A 52-year-old male was admitted due to pain in both hips and for evaluation of hypercalcemia. The plasma level of the intact parathyroid hormone (iPTH) was elevated to 1424 pg/mL. Ultrasonography and the computed tomography revealed a parathyroid cyst on the left thyroid lower pole. Parathyroid scintigraphy detected a parathyroid adenoma. A radiograph showed a subperiosteal bone resorption on the phalanges, and a brown tumor (osteitis fibrosa cystica) on the femur shaft was noted. A surgical excision of the parathyroid adenoma was performed. The PTH level in the cystic fluid was increased. A histological examination confirmed a cystic parathyroid adenoma. The PTH level was normalized after the operation (J Kor SOC Endocrinol 18:214-220, 2003).

Clinical Characteristics and Antimicrobial Susceptibility of Elderly Women with Acute Pyelonephritis

Ji Kang Yun,김웅빈,이상욱,Kwang Woo Lee,Jun Mo Kim,Young Ho Kim 대한요로생식기감염학회 2020 Urogenital Tract Infection Vol.15 No.3

Purpose: Acute pyelonephritis in older patients can lead to a poorer treatment response and prognosis. The objective of this study was to classify older female patients with acute pyelonephritis according to age and compare their clinical characteristics and antibiotic susceptibility. Materials and Methods: In this retrospective study, 360 patients aged 65 years or older and hospitalized for acute pyelonephritis in a single tertiary medical facility from 2012 to 2016 were selected as research subjects. The patients were divided into three groups according to their age. Their clinical symptoms, blood tests and urinalysis, underlying diseases, causative organisms for urinary tract infections (UTIs), and antibiotic susceptibility were compared. Results: One hundred and seventy-six patients aged 69.9±2.7 years, 142 patients aged 79.4±3.1 years, and 42 patients aged 87.3±3.1 years were assigned into the young-old, old-old, and oldest-old groups, respectively. The duration of hospitalization and fever due to acute pyelonephritis increased with age. On the other hand, the maximum body temperature was similar among the three groups. In blood tests, erythrocyte sedimentation rate and C-reactive protein increased with age. Among the underlying diseases, the incidence of diabetes and chronic renal disease was similar among the three groups, while cerebrovascular diseases, heart failure, and urinary stones were associated with aging. Older patients were more likely to have UTIs over the past year. Older groups showed a more significant decrease in sensitivity to carbapenems, amikacin, and ciprofloxacin. Conclusions: Choosing appropriate antibiotics, considering the patientʼs age, is important when treating acute pyelonephritis.

1-Adamantyl Bromide의 압력과 온도의 영향

우지영,박영훈,경진범 한양대학교 이학기술연구소 2001 이학기술연구지 Vol.3 No.-

여러 혼합 용매내에서 1-adamantly bromide의 가용매 분해 반응속도를 여러 온도와 압력하에서 전도도 방법에 의하여 측정하였다. 이들 속도 상수로부터 활성화 부피, ?V_(o)^(≠)활성화 엔탈피, ?H^(≠), 활성화 엔트로피 ?S^(≠)을 구하였다.이때 모든 혼합물내에서 ?V_(o)^(≠) 와 ?S^(≠)는 음의 값을 나타내었으며, ?H^(≠)는 양의 값을 얻었다. 또한 활성화 부피와 활성화 엔트로피 값들을 플로트하여 본 반응에 대한 반응 경향성을 조사하였다. Rates of the solvolysis of l-adamantyl bromide in various mixed solvents have been measured using the conductometric method at various temperatures and pressures. Rate constants obtained by this method were applied to the activated parameters. Values of the activation volumes, ?V_(o)^(≠) and the activation entropies are both negative while values of the activation enthalpies, ?V_(o)^(≠) are positive. The reactivities of these reactions were also estimated from the correlation of the activation volumes with the activation entropies.

외란관측기를 사용한 복합형능동전력필터의 Deadbeat제어에 관한 연구

우정인,박지호,김영빈,문백영 東亞大學校 2000 東亞論叢 Vol.37 No.-

When LC filter is connected parallel to the Active Power Filter, a problem of the resonance between the Active Power Filter(AF) and LC filter often arises. In this paper, deadbeat control of Hybrid Active Power Filter using Disturbance-observer is introduced to solve this problem. By the estimation of the harmonics disturbance using proposed disturbance observer, the compensation characteristics of the AF is improved and the resonant current is suppressed. The validity of proposed disturbance observer is verified from simulation results using PSIM.

레이저 포화흡수체 Nd:LSB에 관한 연구

지명훈,오세용,이영우 목원대학교 멀티미디어신기술연구소 2001 멀티미디어신기술연구소논문집 Vol.1 No.1

본 논문에서는 다른 Q-스위칭용 포화흡수체보다 넓은 천이 영역을 가지고 있는 포화흡수체 Nd:LSB가 Q-스위칭 소자로서 가능한지 보였으며, 최적화된 출력 거울의 반사율, 최대 출력, 출력 에너지, 펄스폭, 출력 효율을 수치적으로 분석하였다. 그 결과 Nd:LSB를 이용한 1319nm 파장의 수동형 Q-스위칭 Nd:YAG 레이저의 특성을 예측할 수 있었고, 공진기 거울의 반사율 98%, 공진기 길이 30㎝로 하였을 경우 출력 31.3MW, 펄스폭 6.22ps를 얻을 수 있다. In this study, it's shown that the saturable absorber Nd:LSB which has broad absorption bandwidth than other saturable absorber as Q-switching system has a capability as a saturable absorber in Q-switching system. In this system, we theoretically analyzed the optimized output mirror, peak power, output power, pulse width and output efficiency. As the result, the passively Q-switched 1319nm Nd:YAG laser using Nd:LSB as a saturable absorber gives the optimized output mirror reflectivity 98% and peak power of 31.3MW and pulse width of 6.22ps at 30㎝ resonator length theoretically.