거세한우의 혈청 및 간장내 비타민 A 농도와 도체형질간의 상관관계

채성화,정근기,최창본 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.4

The current study was conducted to determine the relationships of vitamin A on meat quality of Hanwoo as a basic study to manipulate vitamin A contents in feeds to produce high quality beef Concentrations of vitamin A in scrum and liver of Hanwoo steers(n=328) were analyzed and correlation coefficients with carcass properties were evaluated Mean vitamin A concentration in the serum of Hanwoo steers in the early fattening period was 212.0±32.7 IU/㎗, and that in the late fattening period was 117.56±43.15 IU/㎗. Mean vitamin A concentration in the liver of Hanwoo steers in the late fattening period was 143.62±110 IJ/g expressing large variations might be depended on animals, feeds and farms. There were negative correlations between serum vitamin A concentration and marbling degree(r=-0.24, P<0.01), fat contents in M. Logissimus dorsi (r=-0.21, P<0.01), and beef quality grade(r=-0.20, P<0.01). Vitamin A-palmitate in liver also expressed negative correlations with fat contents in M. Logissimus dorsi (r=-0.18, P<0.01) and beef quality grade(r=-0.16, P<0.05). From the results obtained in the current study, we concluded that it might be possible to produce high quality beef in to determine vitamin A contents in feeds, feeding levels, and feeding periods.

DHPLC 기술을 이용한 돼지 Cytochrome P450 Aromatase 유전자의 조직-특이적 발현양상 관찰

채성화,홍정민,이은주,장종수,최인호 한국동물자원과학회 2004 한국축산학회지 Vol.46 No.3

Clinical Outcome of Rituximab-Based Therapy (RCHOP) in Diffuse Large B-Cell Lymphoma Patients with Bone Marrow Involvement

강병욱,문준호,채의수,이수정,김종광,김여경,이제중,양덕환,김형준,김진영,도영록,박건욱,송홍석,권기영,김민경,이경희,현명수,배성화,김확,손상균,류헌모 대한암학회 2013 Cancer Research and Treatment Vol.45 No.2

Purpose We investigated the clinical outcome of bone marrow (BM) involvement in patients with diffuse large B-cell lymphoma (DLBCL) who received rituximab-based therapy. Materials and Methods A total of 567 consecutive patients with newly diagnosed DLBCL treated with rituximab-CHOP (RCHOP) between November 2001 and March 2010 were included in the current study. All of the patients underwent a BM study at the initial staging and the clinical characteristics and prognosis of these patients with or without BM involvement were analyzed retrospectively. Results The total cohort included 567 patients. The overall incidence of BM involvement was 8.5%. With a median follow-up duration of 33.2 months (range, 0.1 to 80.7 months)for patients who were alive at the last follow-up, the five-year overall survival (OS) and event-free survival (EFS) rate in patients without BM involvement (76.3% and 67.5%,p<0.001) was statistically higher than that in patients with BM involvement (44.3%and 40.1%, p<0.001). In multivariate analysis, among total patients, BM involvement showed a significant association with OS and EFS. In univariate and multivariate analyses, even among stage IV patients, a significant association with worse EFS was observed in the BM involvement group. Conclusion BM involvement at diagnosis affected the survival of patients with DLBCL who received RCHOP. Although use of RCHOP can result in significant improvement of the therapeutic effect of DLBCL, BM involvement is still a negative prognostic factor of DLBCL patients in the era of rituximab.

Expressed Sequence Tags (ESTs) analysis of Tenebrio molitor larvae

정지은,강세원,황희주,채성화,Bharat Bhusan Patnaik,한연수,이재봉,조용훈,이복률,석대현,이용석 한국곤충학회 2013 Entomological Research Vol.43 No.3

Tenebrio molitor has been seriously investigated as a model insect in elucidating Toll signaling pathway and related regulators of innate immunity. However, little is known with regards to the genomic information in T. molitor. In an attempt towards exploiting the rich transcriptomics data that would offer further insights into the study on insect immunity through potential screening of immune‐related genes in the model insect, we constructed a cDNA library (library titer = 5.0 × 105pfu/ml) of T. molitor larvae and analyzed expressed sequence tag (EST) sequences from 1056 clones. The base calling and quality check of obtained chromatogram files were performed by using the Phred program (trim_alt 0.05 (P‐score > 20). After removal of vector and 100 bp and less sequences, 1023 sequences were generated having an average insert size of 792 bp, including 160 clusters, 164 contigs and 387 singletons through clustering and assembling process using the TGI Clustering Tools (TGICL) package. The unique EST sequences were searched against the NCBI nr database by local BLAST (blastx, E < e−5) with 940 sequences showing significant hits. Subsequently, KOG (clusters of orthologous groups for eukaryotic complete genomes) analysis was conducted (blastx, E < e−10) towards prediction of transcriptomal functions, leading to the categorization of 638 genes. The majority of genes belonged to Z category (cytoskeleton‐related genes), R category (general function prediction), and C category (energy production and conversion related genes). These basic EST datasets and their bioinformatics analysis will be helpful in investigating the biological mechanism and molecular pathway related genes involved in innate immunity mechanisms of T. molitor.

왼돌이물달팽이(Physa acuta)의 Metallothionein 유전자 클로닝 및 발현양상

조용훈,백문기,강세원,이재봉,변인선,최상행,채성화,강정하,한연수,박홍석,이용석 한국패류학회 2009 The Korean Journal of Malacology Vol.25 No.3

Metallothioneins (MTs) play a key role in metallic homeostasis and detoxification in most living organisms. In an attempt to study the biological functions and significance of MT in a snail, we cloned and partially characterized the MT gene from the left-handed snail, Physa acutawhich has been regarded as a potential biomonitering species for fresh water. The complete cDNA sequence of PaMT cDNA was identified from the expressed sequence tag (EST) sequencing project of Physa acuta. The coding region of 180 bp gives 60 amino acid residues including the initiation methionine and termination codon. Clustering and phylogenic analysis of PaMT with other MT amino acid sequences show that it has some identities to Helix pomatia (60%), Arianta arbustorum (58%), Perna viridis (49%), Mytilus edulis (49%), Bathymodiolus azoricus (49%), Bathymodiolus azoricus (48%) and Bathymodiolus sp. FD-2002 (48%). Time dependent induction for PaMT from P. acuta exposed with cadmium (50 ppb) indicated that PaMT was induced at 4-8 hr after exposure. It remains to further develop PaMTas a potential biomarker for water contamination in fresh water.

생물정보학을 이용한 연체동물의 NLS (Nuclear Localization Signals) 포함 단백질의 분석Bioinformatic Analysis of NLS (Nuclear Localization Signals)-containing Proteins from Mollusks

이용석,박홍석,조용훈,안인영,한연수,고원규,채성화,강세원,곽희철,최상행 한국패류학회 2006 The Korean Journal of Malacology Vol.22 No.2

localization of a protein containing nuclear localization signals (NLS) has been well studied in many organisms ranging from invertebrates to vertebrates. However, no systematic analysis of NLS-containing proteins available from Mollusks has been reported. Here, we describe in silico screening of NLS-containing proteins using the mollusks database that contains 22,138 amino acids. To screen putative proteins with NLS-motif, we used both predict NLS and perl script. As a result, we have found 266 proteins containing NLS sequences which are about 1.2% out of the entire proteins. On the basis of KOG (The eukaryotic orthologous groups) analysis, we can't predict the precise functions of the NLS-containing proteins. However, we found out that these proteins belong to several types of proteins such as chromatin structure and dynamics, translation, ribosomal structure, biogenesis, and signal transduction mechanism. In addition, we have analysed these sequences based on the classes of mollusks. We could not find many from the species that are the main subjects of phylogenetic studies. In contrast, we noticed that cephalopods has the highest number of NLS-containing proteins. Thus, we have constructed mollusks NLS database and added these information and data to the mollusks database by constructing web interface. Taken together, these information will be very useful for those who are or will be studying NLS-containing proteins from mollusks. 연체동물 유래 아미노산 서열 22,138 개에서 NLS가 예측되는 아미노산 서열은 266 개였으며 이는 연체동물 전체 아미노산 중 1.2% 정도였다. 또한 현재 등재되어 있는 연체동물 8,314 종 중 NLS를 포함한 아미노산이 밝혀진 생물은 60여 종에 불과 하였다. 현재 알려진 연체동물 서열 중에는 두족 강의 경우가 NLS를 포함한 아미노산이 많을 것으로 예측되었다. ACKNOWLEDGEMENT이 연구과제는 1999년도 인제대학교 학술연구조성연구사업의 일환으로 수행되었습니다.

Gene expression profiles analyzed by DNA sequencing of cDNA clones constructed from porcine preadipocytes and adipocytes

이동목,Prati Bajracharya,장은정,이은주,채성화,박홍석,강세원,이용석,이기호,장종수,정정수,최인호 한국유전학회 2012 Genes & Genomics Vol.34 No.2

As a step towards understanding the molecular mechanism of adipogenesis in pigs, preadipocytes purified from the back fat of 1 day-old female piglets were used for in vitro culture. Normalized cDNA libraries were constructed with 1.6×107 and 1.1×107 independent clones from preadipocyte and mature adipocyte mRNAs, respectively. Polymerase chain reaction (PCR)result using primers T3 and T7 (universal primer) confirmed the presence of the insert in the vector. Sequencing of 2,112randomly selected clones from each cDNA library identified 217 clusters, 1,169 singletons, and 216 contigs in preadipocytes and 231 clusters, 1,100 singletons, and 233 contigs in mature adipocytes. Expressed sequence tag (EST) identified 24 genes with known annotation highly expressed in adipocytes and 21 in preadipocytes by at least four EST number. Among those 45 genes, when analyzed by real time RT-PCR,76% of the gene showed significant difference between preadipocytes and mature adipocytes. Highly expressed genes in mature adipocytes were related to adipogenesis, extracellular matrix control and oncogenes, whereas cytoskeleton-related genes were down-regulated. An interesting similarity found during gene profile studies indicated a correlation between cancer and adipogenesis.

증 례 : 중형의 선천성 멜라닌 세포성 모반에서 발생한악성 흑색종의 대상포진 양 피부 전이 1예

김영일 ( Youngil Kim ),김인용 ( Inyong Kim ),채인수 ( In Soo Chae ),봉진구 ( Jin Gu Bong ),정영주 ( Young Ju Jeong ),배성화 ( Sung Hwa Bae ),심정수 ( Jeong Su Shim ),오훈규 ( Hoon Kyu Oh ),박재복 ( Jae Bok Park ),이호준 ( Ho Jun L 대한피부과학회 2015 大韓皮膚科學會誌 Vol.53 No.9

Zosteriform metastasis from malignant melanoma is a rare type of skin metastasis that shows cutaneous lesions including patches, plaques, and nodules along with dermatomes, and thus needs to be distinguished from herpes zoster skin infection. Although some authors have explained the mechanism of zosteriform metastasis, its pathogenesis remains unknown. Herein, we describe an 85-year-old woman with zosteriform metastasis of malignant melanoma arising in a medium-sized congenital melanocytic nevus. (Korean J Dermatol 2015;53(9):708∼712)

한국산 백합 (Meretrix lusoria) 의 전사체 분석

강정하,정지은,김봉석,안철민,강세원,황희주,한연수,채성화,고현숙,이준상,이용석 한국패류학회 2012 The Korean Journal of Malacology Vol.28 No.4

The importance of biological resources has been gradually increasing, and mollusks have been utilized as main fishery resources in terrestrial ecosystems. But little is known about genomic and transcriptional analysis in mollusks. This is the first report on the transcriptomic profile of Meretrix lusoria. In this study, we constructed cDNA library and determined 542 of distinct EST sequences composed of 284 singletons and 95 contigs. At first, we identified 180 of EST sequences that have significant hits on protein sequences of the exclusive Mollusks database through BLASTX program and 343 of EST sequences that have significant hits on NCBI NR database. We also found that 211 of putative sequences through local BLAST (blastx, E < e-10) search against KOG database were classified into 16 functional categories. Some kinds of immune response related genes encoding allograft inflammatory factor 1 (AIF-1), B-cell translocation gene 1 (BTG1), C-type lectin A, thioester-containing protein and 26S proteasome regulatory complex were identified. To determine phylogenetic relationship, we identified partial sequences of four genes (COX1, COX2, 12S rRNA and NADH dehydrogenase) that significantly matched with the mitochondrial genomes of 3 species-Ml (Meretrix lusoria), Mp (Meretrix petechialis) and Mm (Meretrix meretrix). As a result, we found that there was a little bit of a difference between sequences of Korean isolates and other known isolates. This study will be useful to develop breeding technology and might also be helpful to establish a classification system.

동양달팽이 (Nesiohelix samarangae)의 metallothionein 유전자를 기초로 한 분자계통 분류학적 연구

이준서,민병준,강세원,이재봉,백문기,황승영,김소희,고원규,최상행,채성화,박홍석,한연수,이준상,정계헌,이용석 한국패류학회 2008 The Korean Journal of Malacology Vol.24 No.1

Numerous morphological studies on N. samarangae have been well conducted over the last ten years. In this context, we have attemtped to do molecular phylogenetic analysis by using metallothionein (MT) gene from N. samarangae. To this end, we cloned the full length cDNA of MT from cDNA library of N. samarangae. The complete cDNA sequences were obtained from the expressed sequence tag (EST) sequencing project of N. samarangae. The coding region of 195 bp gives an amino acid sequence of 65 residues including methionine. There are 5' (61 bp) and 3' (48 bp) untranslated region at both ends of the Ns-MT cDNA sequence. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of Ns-MT cDNA indicate that N. samarangae has similarity to land snails such as Helix pomatia, Helix aspersa and Arianta arbustorum.