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Cyclic AMP-PKA System이 억제성 및 흥분성 한약재에 의해 유발된 이온전류에 미치는 영향
김연정,신민철,정주호,이충열,김이화,김창주,Kim Youn-Jung,Shin Min-Chul,Chung Joo-Ho,Lee Choong-Yeol,Kim Ee-Hwa,Kim Chang-Ju 경락경혈학회 2001 대한침구의학회지 Vol.18 No.1
Modulatory effect of cAMP-PKA system on inhibitory- and excitatory herbs-induced ion currents were investigated by nystatin-perforated patch clamp method under voltage-clamp condition. Ion currents induced by Bupleuri radix and Coptidis rhizoma were not affected by cAMP-PKA system. Ion current induced by Ecliptae herba was partially inhibited by cAMP-PKA system. Ion currents induced by Aconiti tuber and Boshniakiae herba were inhibitory modulated and ion current induced by Zingiberis rhizoma was excitatory modulated by cAMP-PKA system. Modulation of cAMP-PKA system on ion currents induced by Ginseng radix was fluctuated. In this results, it can be seen generally that excitatory herbs-induced ion currents were modulated by cAMP-PKA system while cAMP-PKA system did not affect inhibitory herbs-induced ion currents.
이침(耳鍼)이 절식(絶食)시킨 흰쥐의 뇌신경세포(腦神經細胞) 활성변화(活性變化)에 미치는 영향(影響)
김이화,김연정,임백빈,장미현,정주호,김창주,Kim, Ee-Hwa,Kim, Youn-Jung,Lim, Baek-Vin,Jang, Mi-Hyun,Chung, Joo-Ho,Kim, Chang-Ju 대한침구의학회 2001 대한침구의학회지 Vol.18 No.1
Objective : The aim of this study was to investigate the effectiveness of stimulation of specific auricular acupuncture points on appetite suppression. Methods : The fasted rats were deprived of food and water for 48 hours. Stimulation of tho inner auricular region of the rat correspond to the human stomach acupuncture point was made for leptin, tryptophan hydroxylase (TPH) and c-fos expression in the brain of the fasted rats by immunohistochemistry. Results : The immunoteactivities of leptin in the periventricular nucleus of the auricular acupunctured group with fasted were significantly higher compared to those of the fasted group. The immunoreactivities of TPH in the median raphe neucleus and reticulotegmental neucleus pons of the auricular acupunctured group with fasted were significantty higher compared to those of the fasted group. And the immunoreactivities of c-fos in the dentate gyrus of the auricular acupunctured group with fasted were significantly lower compared to those from the fasted group. Conclusions : We conclude that the auricular acupuncture altered leptin and TPH-expression in the brain of the fasted rats. The results suggest that auricular acupuncture may inhibit food intake.
김연정,장미현,신민철,임백빈,정주호,반건호,백은경,박재형,김이화,김창주,Kim Youn-Jung,Jang Mi-Hyun,Shin Min-Chul,Lim Baek-Vin,Chung Joo-Ho,Bahn Gyun-Ho,Paik Eun-Kyung,Park Jae-Hyung,Kim Ee-Hwa,Kim Chang-Ju 경락경혈학회 2002 Korean Journal of Acupuncture Vol.19 No.1
목적 : 소아의 정신적 및 신체적 발달지연에 사용되고 있는 신기환이 해마의 신경세포 생성에 미치는 영향을 관찰하고자 하였다. 방법 : 신기환이 세포생성에 미치는 영향을 알아보기 위하여 해마 세포인 HiB5에 신기환을 처리, 배양하여 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay를 통해서 세포생성을 관찰하였다. 또한 3주령의 Sprague-Dawley (S-D)계 흰쥐의 중완혈에 신기환 약침을 자침후 해마의 치상회에서 bromodeoxyuridine (BrdU) immunohistochemistry를 시행하여 세포생성을 관찰하였다. 결과 : HiB5 배양세포에서는 신기환을 처리시 배양시간과 농도별로 세포생성율이 증가하였다. 또한, 대조군의 치상회에서 BrdU 양성세포수는 $128.50{\pm}9.53$, 1 mg/kg 신기환 자침군은 $157.08{\pm}10.82$ 및 10 mg/kg 신기환 자침군은 $204.80{\pm}17.68$로 나타났다. 이러한 결과로 신기환은 어린 S-D계 흰쥐의 치상회에서 세포생성을 증가시키는 것으로 사려된다.
고본(藁本)의 초산으로 유발된 생쥐 통증모델에 대한 마이크로어레이 유전자 발현 양상
김명규,김창주,홍미숙,정주호,부영민,김윤경,홍승헌,임강현,Kim, Myung-Gyou,Kim, Chang-Ju,Hong, Mee-Suk,Chung, Joo-Ho,Boo, Yung-Min,Kim, Yun-Kyung,Hong, Seung-Heon,Leem, Kang-Hyun 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.1
The present study was designed to investigate the effects of Ligustici Rhizoma on the expression of genes in the pain model induced by acetic acid. cDNA microarray (GenePlorer TwinChipTM Mouse 7.4K) was used to evaluate the gene expressions. The expressions of 32 genes were up-regulated in the Ligustici Rhizoma-treated group: they include the genes coding Casp6, Hrh3, Basp1, Sprr2h, Zfp131, Copz2, LOC432436, Itpr5, etc. The expressions of 16 genes were down-regulated in the Ligustici Rhizoma-treated group: they include the genes coding Il16, Zfpm1, Cacna2d1, Xpo7, Smpdl3b, Dscr1, Harp, etc. The conclusion is that the expressions of 32 genes were up-regulated and the expressions of 16 genes were down-regulated in Ligustici Rhizoma-treated group.
이침(耳鍼)이 절식(絶食)시킨 흰쥐의 대뇌피질(大腦皮質)에서 CCK 활성변화(活性變化)에 미치는 영향(影響)
김이화,김연정,임백빈,장미현,정주호,김창주,Kim, Ee-Hwa,Kim, Youn-Jung,Lim, Baek-Vin,Jang, Mi-Hyun,Chung, Joo-Ho,Kim, Chang-Ju 대한침구의학회 2000 대한침구의학회지 Vol.17 No.3
Stimulation of the auricle is known to be effective in reducing obesity. The aim of the present study is to investigate whether stimulating a specific auricular acupuncture point is effective on suppression of appetite. Cholesystokinin (CCK) is known to induce a powerful feeding response after central administration and particularly abundant in the cerebral cortex. In food-deprived rats exhibiting a strong drive for feeding, decreased CCK release was markedly detected in the cerebral cortex of the brain. Needling the fasted rats on the specific auricular region increased the CCK level of the cerebral cortex. In conclusion, stimulating the specific auricular points increases CCK-expression in the cerebral cortex of the fasted rats and may be useful for controlling obesity.
Streptozotocin으로 유발된 당뇨병 흰쥐 대뇌피질에서 침이 Neuronal Nitric Oxide Synthase 발현에 미치는 영향
김이화,황대연,이은용,장미현,김연정,정주호,서정철,김창주,Kim, Ee-Hwa,Hwang, Dae-Yeon,Lee, Eun-Yong,Jang, Mi-Hyun,Kim, Youn-Jung,Chung, Joo-Ho,Seo, Jung-Chul,Kim, Chang-Ju 대한침구의학회 2001 대한침구의학회지 Vol.18 No.5
It has known that acupuncture exerts various effects, such as analgesia, promotion of homeostasis, improvements in brain circulation, and rectification of internal disorders in Oriental medicine. Of particular, acupuncture at the Zusanli acupoint (ST36) has been widely used to relieve symptoms of diabetes mellitus. The effect of acupuncture at Zusanli on neuronal nitric oxide synthase (nNOS)-positive neurons in streptozotocin (STZ)-induced diabetic rats was investigated via immunohistochemistry. Decreased nNOS-positive neurons was detected in cerebrocortex of STZ-induced diabetic rats. Needling on the Zusanli in diabetic rats resulted in increase of nNOS-positive neurons in cerebrocortex, The present study indicate that acupuncture may affect nNOS-positive neurons in cerebrocortex of STZ-induced diabetic rats.
한국인 기관지 천식 허증(虛證), 실증(實證) 환자와 EDN1 유전자 다형성과의 상관성 연구
염유림,김관일,백현정,김미아,이범준,김진주,김수강,정주호,정희재,정승기,Yoem, Yu-rim,Kim, Kwan-il,Baek, Hyun-jung,Kim, Mi-a,Lee, Beom-joon,Kim, Jin-ju,Kim, Su-kang,Chung, Joo-ho,Jung, Hee-jae,Jung, Sung-ki 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.1
Objectives: In the present study, a genetic analysis was conducted to investigate the association of the expression of SNPs of EDN1 gene polymorphism with the clinical phenotype in bronchial asthma patients with either excess or deficiency syndrome.Methods: Ninety-four healthy control subjects and 52 asthma patients were included in this study. The asthma patients were divided into two groups: those with deficiency syndrome and those with excess syndrome. We searched the exonic and promoter areas of the EDN1 gene in the NCBI website SNPs with <0.01 minor allele frequency (MAF) and <0.01 heterozygosity. Pro programs were performed to obtain the odds ratio, 95% confidence interval, and p-value. Multiple logistic regression models were conducted to analyze the genetic data.Results: In our genotype and allele analyses, there were significant differences in the codominant 2 model of the rs3087459 SNP genotype and also in the CGG haplotype between the control group and the asthma group. Genotype and allele analyses were conducted between the deficiency and excess syndrome group. There were significant differences in the dominant and log-additive model and also in the frequency of C-alleles of rs3087459 SNP genotype. There were significant differences in codominant 1, dominant and log-additive model and T-allele of rs5370 SNP genotype. The AGG haplotype also revealed significant differences.Conclusions: EDN1 SNPs (rs3087459, rs5370) showed a significant association with symptomatic excess syndrome in Korean asthmatic patients.
한국인 기관지 천식 허증(虛證), 실증(實證) 환자와 CD46 유전자 다형성과의 관계
이매,백현정,박의근,김관일,이범준,김수강,정주호,김진주,김미아,정희재,정승기,Lee, Mei,Baek, Hyun-jung,Park, Eui-keun,Kim, Kwan-il,Lee, Beom-joon,Kim, Su-kang,Chung, Joo-ho,Kim, Jin-ju,Kim, Mi-a,Jung, Hee-jae,Jung, Sung-ki 대한한방내과학회 2015 大韓韓方內科學會誌 Vol.36 No.4
Objectives In this study, we divided Korean asthma patients into excess syndrome or deficiency syndrome groups according to clinical phenotype. Genetic analysis was conducted to investigate the association of exonic SNPs in the CD46 gene polymorphism with the clinical phenotype based on the differentiation syndrome of the bronchial asthma patients.Methods There were 95 healthy patients (control group) and 53 asthma patients. (The deficiency syndrome group included 24 and the excess syndrome group 29). We searched the exonic areas of the CD46 gene in the NCBI website SNPs with <0.01 minor allele frequency (MAF) and <0.01 heterozygosity. We finally selected two SNPs: rs138843816, Ser13Phe and rs7144, 3’-UTR. Hardy-Weinberg equilibrium was calculated using SNPStats.Results There were significant differences in the codominant 1 model and the dominant model between the healthy group and the asthma group. There were significant differences between deficiency syndrome group and the excess syndrome group in the genotype frequencies and in the codominant 1 model, the dominant model, and the log-additive model. The allele frequency of rs7144C showed a significant difference between the deficiency syndrome group and the excess syndrome group. Two-SNP haplotype analysis showed a significant difference in frequency in the deficiency syndrome group and in the excess syndrome group. There were significant differences between the healthy group and the excess syndrome group in the codominant 1 model, the dominant model, and the log-additive model. The frequency of the rs7144 C allele exhibited a significant difference in the demonstration. SNP haplotype analysis between the healthy group and the excess syndrome group showed a significant difference in the frequency of the CT haplotype and the CC haplotype.Conclusions The results indicate that two CD46 SNPs (rs138843816, Ser13Phe and rs7144, 3′–UTR) might be associated with the symptomatic excess syndrome in Korean asthma patients.