구강위생 증진제용 솔비톨 제조에 미치는 말티톨 및 올리고당의 영향

박영민,조인식,백인섭,정인식 ( Young Min Park,In Shik Cho,In Sup Baik,In Sik Chung ) 한국산업식품공학회 1997 산업 식품공학 Vol.1 No.3

식도에 발생한 다발성 점막교

선희식(Hee Sik Sun),김부성(Boo Sung Kim),이창돈(Chang Don Lee),김인식(In Sik Kim),민창기(Chang Gi Min),최정현(Jung Hyun Choi),서정민(Chong Min Seo),정인식(In Shik Chung) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.2

Mucosal bridge, endoscopically observed, is a cord-like mucosal connection across the lumen, looking very much like a bridge. Mucosal bridges have been infrequently reported in the esophagus, stomach and colon. Recently we experienced a 41-year-old male patient who had multiple esophageal mucosal bridges with duodenal ulcer. We report this case with a review of relevant literatures.

수근관증후근을 동반한 위장관 유전분증

박수헌(Soo Heon Park),박선숙(Sun Sook Park),최명규(Myung Gyu Choi),조세현(Se Hyun Cho),한준열(Jun Yeul Han),최규용(Gyu Yong Choi),정인식(In Shik Chung),선희식(Hee Sik Sun),김부성(Boo Sung Kim),강창석(Chang Suck Kang) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.2

A 58-year old man was admitted to our hospital because of right upper quadrant pain and tingling sensation of both wrists. On physical examination, liver was palpable below thie low costal margin at the right mid-clavicular line. Liver biopsy and gastric mucosal biopsy clemonstrated amyloid deposition. EMG showed the denervated pattern in both median nerves and partial muscular atrophy. On his hospital day, his renal function deteriorated with gastrocolic symptoms. Hemodialysis was initiated. But the patient's condition was not recovered and he died l week after initiation of hemodialysis. Hence, we reported a case of gastrointestinal amyloidosis with carpal tunnel syndrome with the literature. (Korean J Gastroenterol 1995;28: 286 - 291)

간경변증에서 Intrapulmonary Shunting 에 의한 심한 저산소증 1 예

정인식,정규원,신상현,이현재,장진원,김방선,선희식,정환국 대한내과학회 1987 대한내과학회지 Vol.32 No.4

We presented a case of liver cirrhosis with the syn - drome of finger clubbing, cyanosis and polycythemia. A 48 - year - old woman was admitted to the hospital because of severe hypoxemia. On examination, finger clubbing with marked central cyanosis were noted. No cardiac murmurs were audible. The hematocrit was 60.1% and the reticulocyte count 1.9%. The arterial oxygen tension during the breathing of room air was 41 mmHg and the calculated shunt ratio (Qs/Qt) was 16.6%. She had the undrelying Budd-Chiari syndrome demonstrated by inferior venacavogram. The lung funetion test revealed no evidence of obstructive or restrictive lung disease. The echocardiogram showed non-specific findings. The lung perfusion scan using Tc-MAA was compatible with the presence of the intrapulmonary shuntina.

위암에서 에스트로젠 수용체 알파의 소실과 프로모터 과메틸레이션

우인숙,문도호,김성훈,임소희,이명아,강진형,홍영선,이경식,최명규,정인식,박경신 대한내과학회 2004 대한내과학회지 Vol.66 No.5

목적 : 위암에서 에스트로겐 수용체 알파의 프로모터 부위의 메틸레이션으로 인한 위암 조직 내 발현소실이나 불활성화에 대해서는 아직 보고된 바가 없다. 본 연구에서는 위암 세포주와 위암으로 진단 받은 환자들의 조직에서 에스트로젠 수용체 알파의 발현을 평가한 후 위암에서 에스트로젠 수용체 알파의 소실이 5' 프로모터 부위의 메틸레이션과 관련이 있는지를 알고자 하였다. 방법 : 위암 세포주에 대하여 에스트로젠 수용체 알파의 발현을 보기 위하여 웨스턴블롯(12개 세포주)과 RT-PCR(13개 세포주)을 시행하였다. DNA에 대하여 bisulfite modification을 하여 메틸레이션 특이적 중합효소 연쇄반응과 TA 클로닝 후에 염기서열 분석을 시행하였다. 위내시경 조직검사에서 위암으로 진단 받은 42명의 위암 환자의 조직에 대하여 에스트로젠 수용체 알파에 대한 면역조직 화학검사를 시행하였다. 결과 : 위스턴블롯과 RT-PCR결과 KatoⅢ 세포주의 mRNA만 약하게 발현되었으며 모두 음성 반응을 보였다. 메틸레이션 특이적 중합효소 연쇄반응에서 모두 양성이었다. 염기서열 분석에서 ATG 시작 코돈 주변의 CpG island의 methylation을 확인할 수 있었다. 결론 : 위암에서 에스트로젠 수용체 알파의 ATG 시작 코돈주변의 프로모터 부위의 CpG 부위에서 관찰된 메틸레이션은 ER-α유전자의 소실을 일으킬 수 있는 원인의 하나가 될 수 있을 것으로 생각된다. Background : The significance of ER expression and hormone manipulation in gastric cancer is not established. There have been several reports supporting the role of the ER gene as tumor suppressor gene in carcinogenesis. The ER-α gene is located on chromosome 6q25.1. Deletions of the long arm of chromosome 6 are common in gastric carcinoma, suggesting the pressence of tumor suppressor genes in this region. The proportion of ER-positive gastric cancers ranges between 0% and 67% depending on the method of detection. Epigenetic inactivation might explain the loss of ER-α gene expression in gastric cancer. There is no information available regarding the methylation status of the ER-α gene promotor region in gastric cancer so far. The aim of this study was to assess the expression of ER-α in gastric cancer cell lines and determine whether methylation of the 5' promotor region is associated with loss of ER-α expression in gastric cancer. Methods : We investigated such methylation in 13 gastric cancer cell lines. Western blot analysis, reverse transcription-polymerase chain reaction (PCR), methylation-specific PCR (MS-PCR) and bisulfite sequencing analyses were used. Immunohistochemical staining for the ER-α gene was dome for forty-two paraffin embedded tissues from gastric cancer patients. Results : ER-α protein was not detected in any cell line, ER-α mRNA was expressed in only Kato Ⅲline. MS-PCR and bisulfite sequencing showed all thirteen gastric cancer cell lines had methylated CpG regions in their ER-α gene promoters. Immunohistochemical staining of ER-α showed no positivity in any of examined samples. Conclusion : Inactivation of ER-α gene expression in gastric cancer cell lines appears associated with CpG island methylation near the TGA initiation codon of the ER-α gene.

계획된 일정에 의한 식도정맥류 경화요법의 필용성

이봉수,양진모,안병민,정규원,김부성,한준열,선희식,임계순,백남종,정인식 대한소화기내시경학회 1993 Clinical Endoscopy Vol.13 No.1

The Endascopic Injection Sclerotherapy(EIS) has been widely adopted as a primary treatment for acute hemorrhage from esophageal varices. The long term repeated EIS could achieve esophageal variceal obliteration which has laeen regarded as the endpoint of sclerotherapy by many authors. Several studies showed that repeated EIS improved the 1ot18-term survival, and reduced the bleeding episodes. However, in recent prospective randomized trials, the frequency of bleeding was not reduced and even increasd without improvement of survival in patients undergoing prophylactic sclerotherapy. However, after stabilization of acute bleeding soma patients underwent inital sclerotherapy refuse further sclerotherapy mainly due to severe chest pain and several minor complications related to sclerotherapy. Those patients usually undergo sclerotherapy when the eaophaBeal variceral bleeding recur. The aim of this study was to evaluate whether long term repeated sclerotherapy of planned regimen(EP) is superior to episodic sclerosis in regard to the prevention of rebleeding, and improvement in survival. We enrolled 255 patients with an acute variceal bleeding episode within 3 days at the time of admission in this study from Jan 20th 1987 to Aug 20th 1992 in Dae-jeon St. Mary's Hospital. Among 255 patients, 122 patients were excluded from the study because of combined severe illness at the time of initial sclerosis in I6, lost to follows up in 70, inability to classify due to early death in 4, and small varices(below grade 3 by CMC criteria) in 32. Of the total 133 patients, $quot;r5 patients had received endoscopic sclerotherapy by planned regimen(EP) and 58 patients by episodic sclerotherapy on bleeding(EE). Mean follow up was similar in both groups(EP. 23.3±○ months: EE, 25.5±14.2 months). The sclerotherapy of planned reginten reduced rebleeding(EP, 2.01±1.35; EE, 3.90±2.54) and increased overall obliteration rate(EP, 67%; EE, 14%) during the observation period. Cumulative life table analysis revealed no difference in survival between EP and EE. However, in regared to bleeding as a single cause of death, there was a significant difference in survival(P$lt;0.0001). Complication rate was not different between the two groups(EP, 15%; EE, 9%). There was no death related to sclerotherapy in all patients. Esophageal stricture was most frequent complication in two groups, but it was easily treated by TTS ballon dilatation in all cases. At the end of stady there was no difference in hepatic reserve function between the two groups. We conclude that sclerotherapy of planned regaen is more effective to obliterate esophageal varices, to prevent rebleeding than that of episodic method, thus it can reduce the mortality from rebleeding.

위선종 및 위선암 조직에서 Rb , p16 , Cyclin D1 단백질의 발현

최종영,김상우,양영상,정인식,김창섭 대한소화기학회 1998 대한소화기학회지 Vol.32 No.2

Background/Aims: The alterations in cell cycle regulators and subsequent deregulation of the cell cycle are frequently involved in tumorigenesis and/or tumor progression. Methods: In order to evaluate their correlation to gastric carcinogenesis, we analyzed the expression of Rb, p16 and cyclin D1 in 10 cases of human gastric adenomas and 30 cases of gastric adenocarcinomas with various differentiations by immunohistochemistry. Results: Rb shawed immunoreactivity in all 10 cases of adenomas (100%), 8 cases of well-differentiated gastric adenoearcinomas (80%), 6 cases of moderately-differentiated gastric adenocarcinomas (60%) and 2 cases of poorly-differentiated gastric adenocarcinomas (20%). The relationship between the Rb expression and the histologic differentiation of gastric adenomas and adenocarcinomas was statistically significant (P=0.0015). The expression of p16 was observed in 4 cases of poorly-differentiated gastric adenocarcinomas (40%). Cyclin D1 expressed just only 1 case of poorly-differentiated gastric adenocarcinomas. Conclusions: The Rb plays an important role in the differentiation of gastric adenocarcinoma. The role of cyclin D1 in the development and progression of gastric adenocarcinoma would not be important and further studies should be followed to reveal the role of p16 on the gastric carcinogenesis.

간경변증 환자와 복막 암종증 환자의 복수가 정상 호중구의 식균작용과 화학주성에 미치는 영향

강문원,김재광,김호연,안병민,신완식,선희식,정규원,정인식,김부성 대한감염학회 1989 감염 Vol.21 No.4

To assess whether the deficiency of opsonic activity of ascites in lever cirrhosis is predisposed to the development of spontaneous bacterial peritonitis (SBP), routine admission abdominal paracentesis was performed on 39 patients during 10 months. Paracentesis was repeated if the evidence SBP presented during observation period (mean 6.6 months). Among 32 cirrhotic patients 13 patients had never developed SBP (Group I), and 13 patients had developed SBP at least one time during the period (Group Ⅱ), and 6 patients presented SBP on admission (Group Ⅲ). We included 7 patients with peritoneal carcinomatosis of various kind of cancer (Group IV) in this study. The opsonophagocytic and chemotactic activity indices of normal volunteer's polymorphonuclear leukocyte were measured with exposure to ascites of each groups. The ascitic fluid opsonic activity indices of spontaneous bacterial peritonitis at the time of admission (0.43±0.06)was significantly lower than that (0.59±0,07) of sterile cirrhotic ascites (p<0.01), and that (2.05±1.02) of perotoneal carcinomatosis (p<0.001). And the ascites of sterile cirrhotics at the time of admission which, however, had developed SBP during observation period (group Ⅱ) were also found to be significantly decreased opsonic activity indices (0.44±0.06) as compared to group I (p<0.01). The indices of chemotactic activity of same normal volunteer's neutrophil with exposure to ascites of group Ⅱ(0.59±0.13) and group Ⅲ(0.57±0.07) were significantly higher than that (0.48±0.11)of group I (p<0.05), and that (0.82±0.21) of group IV had also significant elevation (p<0.01) as compared to group I, Ⅱ and Ⅲ. These results may explain the prevalence of SBP in cirrhotic ascites is dependent on the opsonic activity of ascites and low opsonic activity of ascites may predispose development of SBP. The elevated chemotaxis of neutrophil with exposure to SBP ascites may result from elevated chmotactic facors in SBP ascites, however, further investigation is needed to elucidate it.