대두 사포닌이 Aflatoxin B₁으로 유도된 세포돌연변이에 미치는 영향에 관한 연구

전혜승(Jun Hye-Seung),성미경(Sung Mi-Kyung) 韓國營養學會 1999 Journal of Nutrition and Health Vol.32 No.1

부랑인시설의 조직 효과성 분석

전혜승(Hye Sung Chun),최재성(Jae- Sung Choi) 한국사회복지행정학회 1999 한국사회복지행정학 Vol.1 No.-

포스터발표 : Aflatoxin B1에 의한 DNA 손상에 대두 사포닌이 미치는 영향에 관한 연구

성미경,전혜승 한국영양학회 1998 추계학술대회초록 Vol.- No.-

B16-F10 Murine Melanoma 세포의 암전이 억제에 미치는 Diallyl Disulfide의 효과

강미경(Mi-Kyung Kang),전혜승(Hye-Seung Jun),염영나(Yung-Na Yum),황명실(Myung-Sil Hwang),박미선(Mi-Sun Park),김옥희(Ok-Hee Kim) 한국독성학회 2006 Toxicological Research Vol.22 No.4

Diallyl disulfide (DADS), an oil-soluble organosulfur compound in garlic has been reported to suppress tumor growth and to induce apoptosis in cancer. In the present study, we investigated the effects of DADS on pulmonary metastasis of B16-F10 murine melanoma cells. DADS (i.p. 40 ㎎/㎏) significantly (p < 0.05) reduced the number of pulmonary metastatic nodules (48%) in experimental pulmonary metastasis assay. We also found that DADS inhibited adhesion, invasion and migration of B16-F10 melanoma cells in a dose-dependent manner. To study the antimetastatic potential of DADS, we performed the effects of DADS on matrix metalloproteinase activity. DADS significantly inhibited the expression of matrix metalloproteinase-2 activity in B16-F10 cells by gelatin zymography. These results suggest that DADS prevent metastasis in part through suppression of migration of B16-F10 melanoma cells by inhibiting matrix metalloproteinase-2 responsible for degradation of extracellular matrix.

비유전독성 발암물질의 검색에 관한 연구(Ⅱ)

김종원,한의식,박미선,엄미옥,전혜승,정해관,오혜영 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품등록의 국제적합의를 위한 협의체(IC딨 . Interna금onal Conference on Harmonization of TecHnological Requirements for Registration of fharrnaceuticats for Human Use)에서 제시하는 유전폭성시험법에서 검색되지 않는 비유전독성 발암물질을 위한 새로운 시험법을 개발하기 위하여 1차년도 연구에서 이러찬 물질에 대한 검색 가능성을 나타낸 '생체외 소핵.시험'과 최근 환경변이원학계에서 새로이 그 유용성이 퍼두되고 있는 'Syrian hamster embryo (SHE) 세포형질전환 시험법'을 이용하여 알려진 두가지 좋류의 비유전독성 발암물질의 반응을 연구하였다. 생체외 소핵시험에서는 2,3,7,8-tetrachlorodibenzo-p-dioxin fTCDD)를 모델화합물로 선정하고, 사람유방 유래의 MCF-7, MCF-lOA,MDA-MB-231 세포를 사용하여 에스트로젠 수용체(ERf 존재여부와 세포의 유형에 파른 소핵형성을 연구하였으며, 등시에 에스트로젠 길항제인 타목시펜에 대한 영향을 보았다. 또한 TCDD가 MCF극 세포의 세포주기예 미치는 영향을 연구하기 위하여 소핵형성을 니-타렌 농도에서 flow cytometer를 이용한 세포주기 분석을 수행하였다. TCDD는 MCF-lOA세포 (ER negative)와 MDA-MB-231세포 (ER negative)에서는 소핵형성이 디미하거나 전혀 증가하지 않았으나, 뽀Cf-7세포 (ER positive)에서 재현성있는 소핵형성을 유발하였으며, 타목시펜 (10 nUt)은 TCDD (IpM)에 의한 소핵형성을 최고 47.3% 까지 억제하였다. 근또글 세포주기분석결과 TCDD, tamoxi(on 단독처리군은 읍성대조군과 유사하몄으나,병용처리군에서는 GO#'Gl arrest를 유의성있게 유발하였다. SHE 세포 형질전환시험법에서는 1차년도 연구에서 생체외 소핵시험에서 양성의 결과를 나타낸 bisphenoB A을 모델화합물로 하여 연구하였다. 시험조건하에서 blsphenol A(31.2SfM, 48시간 연속처리)는 세포혈질전찬을 유발하였다. 상기 연구결과로부터 비유전독성발암물질은 기존의 유전독성시험법중 생체왹 소핵시험(에스트로젠 수용체 양성인 세포주)과 세포형질전환#·1험(Syrian hamster 배자 초대배양세포)에서 시험계의 선택 여부에 따라 부분적으로. 유전독성 지표를 검색할 수 있음을 증명하였으며, 향후 본 연구결과에서 양성을 나타낸 세포의 세포주기 관련인자의 발현변확 등에 대한 작용기전 연구가 진행되어 새로운 생체지표를 이용한 시험법의 개발이 될 것이다 To identify nongenotoxic carcinogens which are not indentified by ICH (Internation Harmonization of I'echnological Requirements ·for Registration of Pharmaceuticals for Human Use) guideline-recommended standard genotoricfty test battery, two endpoints were chosen. They are in vitro micronilcleus 3ss3y which was useful to indentify for bisphenol Aand DEHP last year's study and in vitro 51'rian hamster embryo (SHE) ceBl transformationassay which was recently recognized as a useful tool to detect nongenotoxic carcinogen as wellas genotoxic carcnogen by Environmetal Mutagen Society. In the in vitro micronucleus assayusing MCF-7 cells, TCBD is all negative in standard genotoxicity test battery; Ames test,chromosome abem·ation assay, mouse 3ymphoma tk+/- assay, in vivo micronuclelts assay.therefore TCDD if picked up as a model compound- TCDD atso induced micronucleusformation. To identify the relationship between MN formation and estrogen receptor (ER),TCDO, BPA, and DEHP were studied using hfCF-7 cells (ER positive) and in MCF-tOfL cells(ER negative), MDA-MB-231'ce31s (ER negative). TCDD induced MN formation in bfCF-7celts (ER positive) but not or little induced MN fornlation in MCF-lOA cells (ER negative) andMDA-MB-231 (ER negative). BPA and DEHP didn't induced MN formation in MCF-lOA cells(ER negative). NP didn't induce MN formation in CHt cells but induced MN formation inMCF-7 cells, We also have included the effect of estrogen inhibitors, tamoxifen, against TCDDinduced MN formation. Tamoxifen inhihited TCDD-induced UtN formation up to 4f.3% in,4fCF-7 cells. in cell cycle analysis using flow cytorneter, 1'CDD (1 pM), tamcrifen (10 nM)didn't change cell cycle phase compared with that of negative control but, tamoxifen (10 nhf)with TCDD (1 pM) induced GO/Gl arrest significant]y. Inferring for the result above 4compounds (BPA, EIEHP, TCDD, NP), we concluded the in vitro micronucleus assay vsingMCF-7 cells is a good test method for detecting nongenotoxic carcinogen and identifying thegenotoxicity of endocrine distruptors. In the in vitro SHE celt transformation assay, bisphenol Ais picked up as a model compound. Treated for 48 hr, Bisphenol A induced morphotogicaltransformation significantly.'Strategy and methological approach for identification ofnonBenotoxic cartifogen in vitro'That to identify nongenotcxic carcinogens wllich are notidentified by standard genetic toxicology test battery should be included with additive endpointsIs convincing.

고지방식이를 섭취하는 흰쥐에서 가르시니아캄보지아 껍질추출물, 대두펩타이드 및 L-카르니틴 조성물 섭취와 규칙적인 트레드밀운동이 체중감량에 미치는 영향

김연정(Kim Yun Jung),전혜승(Jun Hye-Seung),박인선(Park In-Sun),김민선(Kim Minsun),이진희(Lee Jinhee),이강표(Lee Kangpyo),박태선(Park Taesun) 韓國營養學會 2005 Journal of Nutrition and Health Vol.38 No.8

현미 및 율무 함유 생식이 영양불균형이 유도된 흰쥐의 체내 지질농도, 항산화체계 및 면역기능에 미치는 영향

박진영(Jinyoung Park),양미자(Meizi Yang),전혜승(Hye Seung Jun),이진희(Jin-Hee Lee),배희경(Hee-Kyung Bae),박태선(Taesun Park) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.2

본 연구에서는 sub-optimal 수준의 비타민과 무기질을 섭취하며, 고콜레스테롤 식이에 알코올 섭취를 병행하여 영양 불균형이 유도된 흰쥐를 대상으로 현미와 율무로 구성된 생식 또는 열처리생식을 식이에 첨가하여 5주간 사육시킨 후 체내 지질농도, 항산화체계 및 면역기능에 미치는 영향을 평가하여 다음과 같은 결과를 얻었다. 영양불균형대조군의 식이효율은 일반대조군에 비해 더 낮았고, 현미 및 율무 함유생식 또는 열처리생식을 첨가한 결과 영양불균형대조군에 비해 식이효율이 유의적으로 증가하였다(p<0.05). 혈청 총콜레스테롤 농도는 일반생식군 또는 열처리생식군에서 영양불 균형대조군에 비해 각각 24% 또는 16% 유의적으로 감소하였고(p<0.05) LDL+VLDL-콜레스테롤 농도 역시 일반생식군에서 영양불균형대조군에 비해 26% 유의적으로 감소하였으나(p<0.05), atherogenic index에는 유의적인 차이가 없었다. 일반생식군의 간조직 콜레스테롤 농도는 영양불균형 대조군에 비해 16% 유의적으로 감소하였다(p<0.05). 간조직의 지질과산화물 농도는 일반생식군 또는 열처리생식군에서 영양불균형대조군에 비해 각각 38% 또는 59% 유의적으로 감소하였다(p<0.05). 한편, 영양불균형대조식이에 일반생식 또는 열처리생식을 첨가한 결과 간조직의 항산화효소계 활성에 유의적인 영향을 미치지 못하였다. 장간막임파절의 CD4^+ T-세포 분포 및 CD4^+/CD8^+ 비율은 일반생식군에서 영양불균형대조군에 비해 유의적으로 증가한 반면(p<0.05), 열처리생식군과 영양불균형대조군 간에는 유의적 차이가 없었다. 이상의 결과를 종합해 보면 열처리를 하지 않은 현미 및 율무 함유 생식은 열처리생식에 비해 영양불균형 흰쥐의 체내 콜레스테롤 농도 저하효과 및 장간막임파절의 면역활성도 증가효과가 더 우수한 것으로 나타냈다. Physiological functions of raw grain diet composed of brown rice and Job’s Tear (1 : 1) were evaluated in rats raised with nutritionally unbalanced diet including 1% cholesterol, high proportion of animal lipids (lard: soybean oil = 8 : 2), sub-optimal levels of vitamin and mineral mixture along with 0.5% ethanol in drinking water for 4 weeks. Control rats were fed the AIN-93G diet for 9 weeks, and nutritionally unbalanced rats were divided into 3 groups, and fed one of the following diets with 0.5% ethanol in drinking water for another 5 weeks: unbalanced control diet (UCD), raw grain diet (RGD)(UCD+20% brown rice and Job's Tear mixture, and cooked grain diet (CGD)(autoclaved RGD at 121℃ for 3 hrs). Feeding UCD for 5 weeks significantly lowered the food efficiency ratio (FER) of rats than the value for control animals, and dietary supplementation of brown rice and Job's Tear mixture to UCD significantly restored the FER. Serum total cholesterol concentration was significantly lowered in rats fed RGD (24% decrease) or CGD (16% decrease) compared to the value for rats fed UCD. Feeding RGD for 5 weeks significaly lowered the serum LDL+VLDL-cholesterol concentration (26% decrease), as well as the hepatic cholesterol level (16% decrease) than the values for UCD rats. Animals fed CGD (38% decrease) or RGD (59% decrease) showed significantly lower level of hepatic thiobarbituric acid reactive substances (TBARS) compared to the value for rats fed UCD (p<0.05), although hepatic activities of antioxidative enzymes were not influenced by dietary supplementation. Feeding RGD for 5 weeks significantly increased CD4^+ T-cell population along with CD4^+/CD8^+ ratio of mesenteric lymph nodes compared to those for UCD rats (p<0.05). In conclusion, dietary supplementation of brown rice and Job's Tear mixture as raw grains exhibited superior activity lowering blood and hepatic levels of cholesterol, and improving mesenteric lymph nodes immune function of rats to the cooked grain mixture of identical ingredients.

Syrian hamster embryo 세포와 mouse embryo BalB/c 3T3 세포에서의 bisphenol A의 세포 형질전환 연구

김종원(Jong Won Kim),한의식(Eui Sik Han),박미선(Mi Sun Park),엄미옥(Mi Ok Eom),전혜승(Hye Seung Jun),민수진(Su Jin Min),김인숙(In Sook Kim),정해관(Hai Kwan Jung),심웅섭(Woong Seop Sim),오혜영(Hye Young Oh) 한국환경성돌연변이발암원학회 2001 한국환경성돌연변이·발암원학회지 Vol.21 No.1

To identify nongenotoxic carcinogen determined as negative by ICH guideline-recommended standard genotoxicity test battery; Ames test, chromosome aberrration assay, mouse lymphoma tk+/- assay, in vivo micronucleus assay, we picked bisphenol A as a model compound. In this study, we applied in vitro BalB/c 3T3 cell transformation assay and Syrian hamster embryo (SHE) cell transformation assay. Bisphenol A was treated upto 769.2 ug/ml in BalB/c 3T3 cells and upto 125 ug/ml in SHE cells. bisphenol A didn't induced morphological transformation both with one stage treatment protocol and with two stage treatement protocol. But, treated for 48 hr, Bisphenol A induced morphological transformation significantly in SHE cells.

Di(2-ethylhexyl) phthalate에 의해 유도된 DNA 손상과 소핵 형성

김종원(Jong Won Kim),한의식(Eui Sik Han),박미선(Hai Kwan Jung),엄미옥(Mi Sun Park),김인숙(Mi Ok Eom),전혜승(In Sook Kim),정해관(Hye Seung Jun),심웅섭(Woong Seop Sim),오혜영(Hye Young Oh) 한국환경성돌연변이발암원학회 2001 한국환경성돌연변이·발암원학회지 Vol.21 No.1

Di-2-ethylhexyl phthalate (DEHP) is the most commonly used phthalate ester in polyvinyl chloride formulations including food packing and storage of human blood. DEHP is a well known as non-genotoxic carcinogen and endocrine disrupting chemical (EDC). DEHP have shown all negative results in ICH-guildeline recommended standard genotoxicity test battery. In this study, to assess the clastogenic and DNA damaging effect in human-derived tissue specific cells, DEHP was treated in human derived MCF-7 cells, HepG2 cells, LNCap cells, BeWo cells, MCF-10A cells, and female peripheral blood cells using micronucleus assay and in human<br/> breast carcinoma MCF-7 cells up to 1.28×10-²M using Comet assay. The in vitro micronucleus assay is a mutagenicity test system for the detection of chemicals which induce the formation of small membrane bound DNA fragment i.e. micronuclei in the cytoplasm of interphase cells, originated from clastogenic and/or aneugenic<br/> mechanism. The single cell gel electrophoresis assay (Comet assay) is used to detect DNA strand-breaks and alkaline labile site. In our results, DEHP increased significantly and/or dose-depentently and time-dependently micronucleus frequency at the 6 and 24 hr without metabolic activation system only in MCF-7 cells. DEHP treated with 2 hrs in MCF-7 cells using Comet assay induced DNA damage dose-depentantly.