대황-황금 혼합물이 만성 역류성 식도염에 미치는 효과

이진아,오민혁,신미래,노성수,박해진,Lee, Jin A,Oh, Min Hyuck,Shin, Mi-Rae,Roh, Seong-Soo,Park, Hae-Jin 한국생약학회 2022 생약학회지 Vol.53 No.1

고지방식이로 유도된 C57BL/6 Mice에서 10가지 천연 소재를 섞은 소스의 비만 억제 효과

이진아(Jin A Lee),박해진(Hae-Jin Park),김수현(Soo Hyun Kim),김민주(Min Ju Kim),김일규(Il Kyu Kim),노성수(Seong-Soo Roh) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.7

본 연구는 천연 항비만 소재를 찾기 위해 4주령 웅성 C57BL/6 mice를 이용하여 정향, 계피, 녹차, 오미자, 울금, 하수오, 상백피, 조릿대, 더덕, 야관문 10가지 천연 소재 복합물의 항비만 효과를 확인하였다. 고지방식이와 함께 10가지 한약재를 동일한 비율로 증류수에 혼합한 ‘SME’와 소스 베이스에 혼합한 ‘SMS’를 6주간 공급하였다. SME 및 SMS 투여를 통해 몸무게가 유의적으로 감소하는 효과가 나타났으며, 혈액 내 활성산소의 유의적인 감소가 나타났다. 간 조직 및 분변 내 TG, TC 수치 감소가 나타났으며, 또한 혈액 내 TG, TC, HDL-C 및 LDL-C 수치를 감소시켰다. 천연 소재 복합물은 AMPK의 활성화를 통해 p-AMPK 발현을 증가시킴으로써 SREBP-1c의 생성을 감소시키고 ACC의 인산화를 촉진했으며, SREBP-1c의 표적인자인 FAS 및 SCD-1의 발현 또한 유의적으로 감소시켰다. 실험 결과 SME 및 SMS의 항비만 효과를 확인하였으며, 소스 베이스에 천연 복합물 소재를 혼합한 SMS는 항비만 천연 소재 식품 개발에 있어서 활용 가능성이 높을 것으로 기대된다. This study investigated the anti-obesity effects of natural material complexes. Male C57BL/6 mice were divided into four groups: a normal diet group (Nor), a high fat diet group (Con), 10 kinds of herbal medicines mixed with distilled water (SME), and mixed with sauce base (SMS). After 6 weeks, the body weight of the Con group increased and that of the SME group and the SMS group decreased. In addition, serum reactive oxygen species production was significantly decreased, and decreased alanine aminotransferase and aspartate aminotransferase were observed. Triglyceride and total cholesterol levels in serum and liver tissue were decreased, and the size of adipocytes was decreased in liver tissue of the treated group. Additionally, expression of proteins such as sterol regulatory element-binding transcription factor 1c, fatty acid synthase, and stearoyl-CoA desaturase-1 was down-regulated in the administration group. Overall, the results suggested that the mixture of 10 herbal medicines has anti-obesity effects.

진피-황금 혼합물이 급성 역류성 식도염 흰쥐에 미치는 효과

이진아(Jin A Lee),신미래(Mi-Rae Shin),노성수(Seong-Soo Roh),박해진(Hae-Jin Park) 한국영양학회 2021 Journal of Nutrition and Health Vol.54 No.3

본 연구에서는 진피-황금 혼합물 (CS)이 급성 역류성 식도염에 미치는 식도 점막 보호 효과를 평가하기 위하여 CS를 경구투여한 후 수술을 통해 역류성 식도염을 유발하였으며, 실험 종료 후 혈액 채취 및 식도 조직을 적출하였다. 동물에게서 적출한 식도 점막의 손상 정도를 육안으로 확인한 결과 CS투여군에서 식도 점막의 손상이 유의하게 감소하였으며, H&E staining을 통해 관찰한 결과 마찬가지로 CS투여군에서 식도 상피의 탈락 및 염증세포의 침윤이 현저하게 감소한 것을 확인하였다. 혈액을 이용하여 역류성 식도염의 원인으로 유효하다고 알려진 ROS의 수치를 확인한 결과, CS투여군에서 ROS 수치가 유의적으로 감소하였으며, western blotting을 통해 NADPH oxidase인 NOX4, p47<SUP>phox</SUP>, p22<SUP>phox</SUP>의 발현을 확인한 결과, 마찬가지로 CS 투여군에서 유의하게 감소하였고, 특히 CS200투여군에서 Normal군과 비슷한 수치를 나타냈다. 또한, CS투여는 염증성 단백질인 MAPK와 NF-κB 경로를 유의적으로 억제하였을 뿐 아니라 tight junction 단백질인 claudin-1과 claudin-4의 발현을 유의하게 조절한 것을 확인하였다. 이상의 결과를 종합해보면 진피-황금 추출물은 산화적 스트레스를 억제함으로써 염증성 단백질의 발현을 조절할 뿐 아니라 tight junction 단백질의 발현을 조절하여 식도 점막을 보호하는 것으로 판단되나 역류성 식도염은 음식물의 섭취와 밀접한 관련이 있는 만큼 추후 동물의 식이 섭취량을 조사하는 등 세부적인 추가 연구가 필요할 것으로 보인다. Purpose: Reflux esophagitis is a disease caused by the reflux of stomach contents and stomach acid etc. into the esophagus due to defect in the lower esophageal sphincter and is currently increasing worldwide. This study was conducted to evaluate the effect of a mixture of Citrus Reticulata and Scutellariae Radix (CS) extract on acute reflux esophagitis in rats. Methods: Rats were divided into five groups for examination: normal group (Normal, n = 8), water-treated acute reflux esophagitis rats (Control, n = 8), tocopherol 30 mg/kg body weight-treated acute reflux esophagitis rats (Toco, n = 8), CS 100 mg/kg body weight-treated acute reflux esophagitis rats (CS100, n = 8), CS 200 mg/kg body weight-treated acute reflux esophagitis rats (CS200, n = 8). The experimental groups were administrated of each treatment compounds and after 90 min, acute reflux esophagitis was induced through surgery. Rats were sacrificed 5 h after surgery. We measured the level of reactive oxygen species (ROS) in serum and analyzed the expression of nicotinamide adenine dinucleotide phosphate, inflammatory, and tight junction-related proteins by western blot in the esophageal tissues. Results: CS administration significantly protected the esophageal mucosal damage due to reflux esophagitis, and the level of ROS in the serum was significantly reduced with CS administration as compared to Control. In addition, CS administration significantly suppressed mitogen-activated protein kinase (MAPK or MAP kinase) and nuclear factor-kappa B (NF-κB) pathways and increased protein expressions of tight junction protein. Conclusion: These results suggest that the CS not only regulates the expression of inflammatory proteins by inhibiting oxidative stress, but also reduces damage to the esophageal mucosa by inhibiting the expression of tight junction proteins.

각막반사와 사진을 이용한 새로운 사시각 측정법

이진아 ( Jin A Lee ),이해영 ( Hae Young Lee ) 대한안과학회 2002 대한안과학회지 Vol.43 No.10

애엽(艾葉) 추출물이 알코올성 위염에 미치는 효과

이진아,서정복,김진영,신미래,박해진,노성수,Lee, Jin A,Seo, Jeong Bok,Kim, Jin Young,Shin, Mi-Rae,Park, Hae-Jin,Roh, Seong-Soo 대한한방내과학회 2022 大韓韓方內科學會誌 Vol.43 No.4

Objective: Alcohol is known to cause inflammation in the stomach by decreasing the protective substances of the gastric mucosa and increasing oxidative stress. The purpose of this study was to investigate the anti-inflammatory effect of Artemisiae Argyi Folium extract (AF) on alcohol-induced gastritis. Methods: The total polyphenol and flavonoid contents of AF were confirmed through an in vitro experiment. Radical scavenging activities were confirmed using DPPH and ABTS assays. In vivo experiments were conducted on mice divided into 5 groups (n=8): a normal group (Nor), an alcohol-induced gastritis group (Con), an alcohol-induced gastritis group administered 10 mg/kg sucralfate (SC), an alcohol-induced gastritis group administered 100 mg/kg AF (AFL), and an alcohol-induced gastritis group administered 200 mg/kg AF(AFH). The serum levels of reactive oxygen species (ROS) were determined, and protein expressions were confirmed in gastric tissues. Results: In alcohol-induced gastritis, AF alleviated damage to the gastric mucosa caused by alcohol. AF also decreased the serum ROS levels. Western blots showed that AF decreased the expression of NADPH oxidase and decreased the expression of the NF-κB pathway associated with inflammation. AF also decreased the expression of adhesion molecules and chemokine proteins, and increased the expression of anti-inflammatory proteins. Conclusions: AF not only reduced oxidative stress in alcohol-induced gastritis, but it also relieved gastric mucosal inflammation by regulating the expression of the NF-κB pathway.

개방형 하드웨어 플랫폼 기반의 확장 용이한 스마트 홈 IoT 시스템

이진해,박광일,신종하,유성은,Lee, Jin-hae,Park, Gwang-il,Shin, Jong-ha,Yoo, Seong-eun 대한임베디드공학회 2016 대한임베디드공학회논문지 Vol.11 No.6

Recently, many changes have been made to people's life patterns as the technological advances in the ICT industry. The fusion of smart phones and various IT technologies has brought people convenience and welfare. A typical example of such fusion is the smart home. However, the existing smart home systems are difficult to be changed or extended. So we design a new smart home system with extensibility that can easily adopt legacy appliances and be scaled up. Among a variety of smart home features, this paper deals with IoT Devices that are responsible for controlling power or transmitting and receiving sensing values, IoT Gateway that connects users and consumer electronics via Internet, and Smart Home Manager that monitors and controls these components in the proposed smart home system.

높은 측위 신뢰도 유지를 위한 주행 환경별 위치 추정 융합 시스템의 스위칭 알고리즘

이진해(Jin-Hae Lee),윤식(Sik Yoon),정재일(Jae-Il Jung) 한국자동차공학회 2018 한국자동차공학회 부문종합 학술대회 Vol.2018 No.6

USB 장치 정보를 이용한 사용자 인증방안

이진해(Jin-Hae Lee),조인준(In-June Jo),김선주(Seon-Joo Kim) 한국콘텐츠학회 2017 한국콘텐츠학회논문지 Vol.17 No.7

ID/PW(Identifier/Password) 인증방식은 다양한 보안취약성이 존재함에도 사용이 편리하고 구축비용이 저렴하여 아직까지 폭 넓게 사용하고 있으며, 유추해내기 어려운 복잡한 패스워드의 사용과 주기적인 변경을 요구하고 있다. 하지만 사용자 입장에서는 복잡한 패스워드를 기억하고 주기적으로 변경하는 것은 매우 불편한 일이다. 이에 본 논문에서는 USB(Universal Serial Bus) 메모리를 활용하여 복잡한 패스워드를 주기적으로 변경할 필요가 없는 인증시스템을 제안하였다. 사용자 인증시마다 자동으로 재생성 되도록 하고 기존의 인증데이터는 재사용이 불가능하도록 설계하였다. 이를 바탕으로 ID/PW 인증시스템을 크게 고치지 않으면서 사용이 편리하고, 인증서 / 지문인식 수준의 보안성을 제공할 수 있다. Password-based authentication is vulnerable because of its low cost and convenience, but it is still widely used. In order to increase the security of the password-based user authentication method, the password is changed frequently, and it is recommended to use a combination of numbers, alphabets and special characters when generating the password. However, it is difficult for users to remember passwords that are difficult to create and it is not easy to change passwords periodically. Therefore, in this paper, we implemented a user authentication system that does not require a password by using the USB memory that is commonly used. Authentication data used for authentication is protected by USB data stored in USB memory using USB device information to improve security. Also, the authentication data is one-time and reusable. Based on this, it is possible to have the same security as the password authentication system and the security level such as certificate or fingerprint recognition.

주행 상황별 Quality에 따른 최적화된 GNSS+INS/UWB+INS/INS 시스템 Switching 알고리즘에 관한 연구

이진해(Lee Jin-Hae),정재일(Jung Jae Il) 한국통신학회 2018 한국통신학회 학술대회논문집 Vol.2018 No.1

급성 위염 동물 모델에서 감국(甘菊) 추출물과 아마인유(亞麻仁油) 혼합물의 위 점막 보호 효과

이진아 ( Jin A Lee ),김수현 ( Soo Hyun Kim ),김민주 ( Min Ju Kim ),안정현 ( Jeong-hyun Ahn ),박해진 ( Hae-jin Park ),이우락 ( Woo Rak Lee ),노성수 ( Seong-soo Roh ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.6

Objectives : The objective of this study was to investigate the protective effect of Flaxseed oil and Chrysanthemi Indici Flos 50% ethanol extract in an HCl/ethanol induced acute gastritis model. Methods : ICR mice were divided into 6 groups; normal mice (Nor), gastritic mice with distilled water (Veh), gastritic mice with 10 ㎎/㎏ sucralfate (SC), gastritic mice with 16 g/㎏ Flaxseed oil (FO), gastritic mice with FO + 50 ㎎/㎏ Chrysanthemi Indici Flos (FCL), and gastritic mice with FO + 100 ㎎/㎏ Chrysanthemi Indici Flos (FCH). Then, mice were orally administered with 150 mM HCl/60% ethanol and caused acute gastritis. After 1 hr, mice were sacrificed, and blood and stomach tissue were collected. Results : Administration of FCL and FCH to mice prior to the induction of gastritis was found to reduce gastric injury. reactive oxygen species (ROS) and peroxy nitrite (ONOO^-) levels of stomach tissues were significantly decreased in FO, FCL, and FCH compared to Veh group. As results of stomach protein analyses, FCL and FCH effectively reduce inflammatory-related factors such as inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), and interleukin 1 beta (IL-1β) in gastric lesion mice. In addition, nuclear factor kappa B p65 (NF-κB p65) and phosphorylation inhibitor of nuclear factor kappa Bα (p-IκBα) were down-regulated in FCL and FCH administrated gastric lesion mice. Conclusions : These results suggest that FCL and FCH has an inhibitory effect against gastric injury. Therefore, FCL and FCH has the potential to be used as a natural therapeutic drug.