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감태(Ecklonia cava) 줄기 및 잎의 효소적 추출물과 메탄올 추출물에 의한 항산화 활성비교
이승홍(Seung-Hong Lee),김길남(Kil-Nam Kim),차선희(Seon-Heui Cha),안긴내(Gin-Nae Ahn),전유진(You-Jin Jeon) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.9
본 연구에서는 제주 연안에 서식하는 갈조류 중 감태의 줄기와 잎의 항산화활성을 비교하기 위하여 감태 줄기와 잎으로부터 효소적 가수분해와 메탄올을 이용하여 각각의 추출물을 제조한 후 이들로부터 활성산소종의 소거활성 및 세포의 산화적 손상 억제활성을 포함하는 항산화활성에 대한 차이를 비교 검토하였다. 여기에서 효소적 가수분해를 이용하여 제조된 감태의 효소적 추출물은 메탄올 추출물에 비하여 폴리페놀 함량은 낮았으나, 수율은 약 30% 이상 높게 나타났다. 감태는 줄기와 잎의 모든 부분에서 항산화활성이 우수하게 나타났으며, 두 부위 간의 유의적인 차이는 없었으나, 대체로 잎의 추출물이 줄기의 추출물보다 약간 우수한 항산화효과를 나타내었다. 이러한 결과는 감태의 잎 뿐만 아니라 줄기 또한 마찬가지로 우수한 생리활성 재료임을 확인할 수 있었다. 그리고 메탄올 추출물에 비해 효소적 추출물은 hydroxyl radical 소거활성을 제외한 전반적인 항산화 효과 분석에 있어서 더 유용한 경향을 보였다. 이와 같은 결과는 여러 가지 효소들이 복합되어진 효소가 세포벽에 있는 섬유질이나 당단백질 혹은 알긴산 고분자물질 등을 분해 시키는 작용을 하여 활성물질들이 원활히 추출될 수 있도록 유도해 주는 작용을 하였기 때문이라고 사료된다. 그리고 높은 수율을 바탕으로 한 수용성의 추출물이기 때문에 그들이 가지고 있는 생리활성물질을 식품산업에 쉽게 응용시킬 수 있으며, 유기용매와 같은 화학약품을 사용하였을 때에 발생할 수도 있는 안전성에 대한 문제도 해결할 수 있을 것이다. 이상의 결과로 감태는 잎 뿐만 아니라 줄기도 잠재적 의약품 소재 및 기능성식품 소재로서의 가능성이 충분하다고 판단된다. In this study, antioxidant activities of enzymatic and methanolic extracts from E. cava stem and leave were evaluated by measuring the scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical, hydrogen peroxide and the inhibitory effects on DNA damage induced by oxidative stress of cells. Enzymatic extracts were prepared by enzymatic hydrolysis of both stem and leave using food grade five different carbohydrases (Viscozyme, Celluclast, AMG, Termamyl, Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme, Alcalase). The enzymatic extracts were lower than methanolic extracts in polyphenol contents, but higher in extraction yield by approximately 30%. The enzymatic extracts were superior to methanolic extracts in DPPH and H₂O₂ scavenging activities and DNA damage protective effect. There were no significant antioxidant activity difference between stem and leave, but the extracts of leave were relatively better than those of stem. In this study it is suggested that E. cava stem as well as its leave would be a good raw materials for antioxidants compound extraction and enzymatic hydrolysis would be a good strategy to prepare antioxidant extracts from seaweeds.
직사각형 프리즘 주위의 유동구조에 대한 경계층 두께의 영향
지호성,김경천,이승홍,부정숙,Ji, Ho-Seong,Kim, Kyung-Chun,Lee, Seung-Hong,Boo, Jeong-Sook 대한기계학회 2002 大韓機械學會論文集B Vol.26 No.6
Effect of boundary layer thickness on the flow characteristics around a rectangular prism has been investigated by using a PIV(Particle Image Velocimetry) technique. Three different boundary layers(thick, medium and thin)were generated in the Atmospheric Boundary Layer Wind Tunnel at Pusan National University. The thick boundary layer having 670 mm thickness was generated by using spires and roughness elements. The medium thickness of boundary layer($\delta$=270 mm) was the natural turbulent boundary layer at the test section floor with fairly long developing length(18 m). The thin boundary layer($\delta$=36.5 mm) was generated on the smooth panel elevated 70cm from the wind tunnel floor. The Reynolds number based on the free stream velocity(3 ㎧) and the height of the model(40 mm) was 7.9$\times$10$^3$. The mean velocity vector fields and turbulent kinetic energy distributions were measured and compared. The effect of boundary layer thickness was clearly observed not only in the length of separation bubble but also in the location of reattachment point. The thinner the boundary layer thickness, the higher the turbulent kinetic energy Peak around the model roofbecame. It is strongly recommended that the height ratio between the model and the approaching boundary layer thickness should be encountered as a major parameter.
주변공기조건이 충돌수분류에 의한 고온강판의 냉각에 미치는 영향 연구
이필종,최해원,이승홍,Lee, Pil-Jong,Choi, Hae-Won,Lee, Seung-Hong 대한기계학회 2000 大韓機械學會論文集B Vol.24 No.1
It is observed that the cooling capacity of impinging water jet is affected by the seasonal conditions in steel manufacturing process with large scale. To confirm this phenomena, the cooling experiments of a hot steel plate by a laminar jet were conducted for two different initial ambient air temperature($10^{\circ}C$ and $40^{\circ}C$) in a closed chamber, and an inverse heat conduction method is applied for the quantitative comparison. It is found that the cooling capacity under $10^{\circ}C$ air temperature is lower than that under $40^{\circ}C$, as is the saturated water vapor is more easily observed, and the amount of total extracted heat in the case of $10^{\circ}C$ is smaller by nearly 15% than that of $40^{\circ}C$ case. From these results, it is thought that the quantity of water vapor, which could be absorbed until saturation, effects on the mechanism of boiling heat transfer.
이필종,최호,이승홍,Lee, Pil-Jong,Choi, Ho,Lee, Seung-Hong 대한기계학회 2002 大韓機械學會論文集B Vol.26 No.6
The cooling heat transfer by impinging water spray jets on a rotating roll with a relatively large diameter has been investigated under various experimental conditions with 3 different sizes of flat type nozzle. The local heat transfer coefficients were calculated by finite difference method using measured surface temperatures of the circular cylinder as boundary conditions. Results show that a peak value of the heat transfer coefficient is located at the center of sprayed area and there may be a secondary peak at the downstream. The average heat transfer coefficients on the sprayed area were found to be 10 to 22 ㎾/$m^2$$^{\circ}C$, and were not related to spraying pressure, but approximately linearly to flow rate of sprayed water. Also it is found that increasing the distance from roll to nozzle could improve the cooling efficiency by increasing the sprayed area.
제주산 우뭇가사리(Gelidium amansii )로부터 DMSO 추출과 EDTA 수세법에 의한 상용화 아가로스 제조
강태환 ( Tai Hwan Kang ),이승홍 ( Seung Hong Lee ),백종석 ( Jong Seok Baik ),강병식 ( Byung Sik Kang ),이정석 ( Jung Suck Lee ),이남호 ( Nam Ho Lee ),전유진 ( You Jin Jeon ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6
Agar was prepared from Gelidium amansii collected from Jeju Island, South Korea. This agar preparation has high gel strength and low sulfate content compared with G. amansii agar from Morocco. Accordingly, agarose was made from the Jeju agar through the consecutive refining processes of dimethyl sulfoxide (DMSO) extraction and ethylene diamine tetra acetic acid (EDTA) washing. The physicochemical properties of the resulting agarose were compared with those from agarose prepared using only DMSO extraction. Consecutive DMSO extraction and EDTA washing more strongly affected the physicochemical properties of the agarose (purified agarose) compared with the use of DMSO extraction alone. These properties were similar to those of commercial agarose used for electrophoresis. In DNA electrophoresis, the separation and movement speed of the purified agarose were similar to those of the commercial agarose. In a 13C NMR analysis, the purified agarose exhibited the same carbon peak as the commercial agarose. When observed under scanning electron microscopy, the agar had an even and smooth surface without irregularities or pores, and the purified agarose had a wide surface area with a large number of pores; the commercial agarose had an irregular surface that would allow the solvent to easily permeate. These results illustrate that the physicochemical properties of agarose prepared from DMSO extraction and EDTA washing were more effective than those observed after DMSO extraction alone; thus, these processes used in succession will be useful in agarose industries.
최형림(Hyung-Rim Choi),이승홍(Seung-Hong Lee),박용성(Yong-Sung Park),강무홍(Moo-Hong Kang),김희윤(Hee-Yoon Kim),최기남(Ki-Nam Choi),주이돈(Yi-Don Joo),하정수(Jeong-Su Ha) 한국산업정보학회 2009 한국산업정보학회논문지 Vol.14 No.5
전 세계적으로 지속가능한 발전을 위한 그린 경영에 관심이 집중되고 있다. 국내에서는 정부의 저탄소 녹색성장 정책의 지원에 따라 항만분야에서도 탄소배출량의 감소와 운영비용의 절감을 목적으로 운영을 위해 필수적으로 사용되는 Crane의 주 동력원을 화석연료에서 전기로 전환하기 위한 노력을 기울이고 있다. 그 중에서도 e-RTGC는 이미 연구를 통해 그 우수성이 검증되고 필요성 또한 높은 것으로 평가되었으나, 실제 터미널에 도입하였을 때 e-RTGC의 효과에 대한 실증분석 연구는 전무하여 그 효과가 입증되지 못한 실정이다. 이러한 가운데 최근 국내 여러 터미널에 e-RTGC가 실제로 도입되고 운영을 하게 됨으로써, 그 동안 부족했던 자료의 충족과 새로운 연구를 위한 활로의 개척이 이루어졌다고 할 수 있다. 이에 본 논문에서는 e-RTGC를 도입한 D터미널을 대상으로 실증분석연구를 진행하여, 컨테이너터미널의 e-RTGC 도입 시 고려되어야 하는 제약 요인과 실제 운영을 통해 얻은 효과와 문제점을 조사 및 분석하고자 한다. 이 연구를 통해 얻을 수 있는 기대효과로는 e-RTGC로 전환하고자 하는 터미널에 중요하게 고려되어야 하는 요인과 실증분석자료를 제공할 수 있을 것이라는 것과, 향후 e-RTGC의 발전 방향에 대한 참고자료로 활용될 수 있다는 점이다. All over the world more and more attention is being paid to green management which enables environment-friendly sustainable development. To keep up with these global trends and in response to the government’s “low carbon green growth” policy, many efforts are being made in the port and logistics in order to drastically reduce carbon emissions and save operating expenses. To this end, the engine power source of the cranes in the container terminal is being replaced with electricity from fossil fuel. Among those cranes, especially e-RTGC has been proved to be practical and excellent in its performance through many previous studies. However, no empirical study has yet been made on its introduction effects when e-RTGC has been introduced to the container terminal. In the meantime, however, many domestic container terminals have introduced e-RTGC, and so some data have been accumulated for further research. Under these circumstances, this study has tried to make an empirical study through the case study of D container terminal, checking the restriction factors to be considered at the time of e-RTGC introduction, and analyzing its introduction effects and problems. We have high expectations that this study will provide key consideration factors and empirical analysis data to the decision makers who plan to introduce an e-RTGC system, and also will be used as a reference to the development direction of e-RTGC.