인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

대장직장 종양에 대한 내시경 점막하 절제술의 연대순 임상 결과와 학습 곡선

이상민 ( Sang Min Lee ),김은수 ( Eun Soo Kim ),박경식 ( Kyung Sik Park ),조광범 ( Kwang Bum Cho ),김동춘 ( Dong Choon Kim ),강유진 ( Yu Jin Kang ),이유진 ( Yoo Jin Lee ),이정민 ( Jung Min Lee ),최은성 ( Eun Sung Choi ),최재혁 ( Ja 대한소화기학회 2014 대한소화기학회지 Vol.64 No.4

Background/Aims: Endoscopic submucosal dissection (ESD) is an effective procedure for en-bloc curative resection of the colorectal tumor. As it requires high technical skills and experience in therapeutic endoscopy, it is important to understand learning curve of ESD technique. The aim of this study was to retrospectively describe the clinical results of ESD and to evaluate learning curve for the ESD of colorectal tumors. Methods: A total of 90 patients with 90 colorectal neoplasms, who had undergone ESD at a tertiary referral hospital from July 2009 to December 2012, were enrolled. The ESD was performed by a single endoscopist. All ESD cases were divided into three periods: first, cases 1-30; second, cases 31-60; and third, cases 61-90. Results: The en-bloc resection rates in third period (100%) was significantly higher than that of the first (93.3%) and second period (80%) (p=0.025). The perforation rate in third period (0%) also significantly decreased compared with that of the first (13.3%) and second period (20%) (p=0.032). To calibrate the difference of tumor size among periods, proficiency was calculated, as the procedure time per specimen area (min/cm2). The proficiency in third period (4.3) was significantly shorter than that of the first (16.8) and second period (10.2) (p=0.004). Conclusions: The learning curve of colorectal ESD in our study shows that at least 60 cases of ESD have to be conducted to acquire sufficient skill of degree without perforation. (Korean J Gastroenterol 2014;64:198-205)

대식세포주에서 인슐린이 $I{\kappa}B/NF-{\kappa}B$ 경로 활성화에 미치는 영향

이상민,장연실,이춘택,김영환,한성구,심영수,유철규,Lee, Sang-Min,Jang, Yeon-Sil,Lee, Choon-Taek,Kim, Young-Whan,Han, Sung-Koo,Shim, Young-Soo,Yoo, Chul-Gyu 대한결핵및호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.3

Background: Sepsis still has a high mortality rate despite adequate supportive care. Newer therapeutic modalities have been developed but they have generally ended in failure. Recently, insulin was reported to have an anti-inflammatory effect by inhibiting the $I{\kappa}B/NF-{\kappa}B$ pathway, and may have therapeutic potential in sepsis. However, the precise mechanism of the anti-inflammatory effect of insulin is unclear. This study examined the role of insulin in activating $I{\kappa}B/NF-{\kappa}B$ in macrophage. Methods: Raw 264.7 cells, a murine macrophage cell line, were used in this experiment. Western blotting using $I{\kappa}B$ Ab and phosphor-specific $I{\kappa}B$ Ab was performed to evaluate the degradation and phosphorylation of $I{\kappa}B$ cells. For the $I{\kappa}B$ Kinase (IKK) activity, an immune complex kinase assay was performed. The level of interleukin-6 (IL-6) was measured by ELISA to determine the level of proinflammatory cytokine. Results: $I{\kappa}B{\alpha}$ degradation began 30 min after lipopolysaccharide (LPS) treatment. However, an insulin pretreatment suppressed the $I{\kappa}B{\alpha}$ degradation caused by the LPS treatment. The phosphorylation of $I{\kappa}B{\alpha}$ and IKK activity was also inhibited by the insulin pretreatment. Finally, the insulin pretreatment showed a tendency to suppress the induction of IL-6 by LPS. Conclusion: Insulin might have an anti-inflammatory effect though partial inhibition of the $I{\kappa}B/NF{\kappa}B$ pathway in macrophage cell lines.

헤어리베치(Vice villosa Roth) 품종별 국내 월동성, Biomass 및 무기태 질소 함량비교

이상민,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2013 한국유기농업학회지 Vol.21 No.1

This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

녹비작물을 이용한 윤작이 잡초 제어에 미치는 영향

이상민,이병모,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Byung-Mo,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2012 한국유기농업학회지 Vol.20 No.2

This study was conducted to evaluate the effects of crop rotation on the weed occurrence in green onion and red pepper cultivation. Rye and hairy vetch were annually seeded for winter season. The reduction of weed germination was pronounced by about 50% in green onion field, but was not significant in red pepper field through rye cropping. Among weed species, the germination of Abutilon theophrasti, Cyperus iria and Digitaria ciliaris was distinctly inhibited by exudate of rye root, but not by that of hairy vetch in vitro experiment. The return of rye biomass into onion field decreased amount of weed biomass but increased index of weed diversity.

녹색건축 전문가 자격제도 개발 연구

이상민,이승민,Lee, Sang Min,Lee, Seung Min 한국교육녹색환경연구원 2014 교육·녹색환경연구 Vol.13 No.1

In an effort to resolve environmental pollution problems, developed countries enforce green building certification systems. The same is true in Korea. However compared professional accreditation system to certification systems did not systematically in Korea. Therefore, in accordance with development of green building related systems and technology the requirement for foster green building professionals is urgent. Accordingly this study is purposed to analyze and propose that green building professional accreditation system development and operation of the direction. Through data analysis domestic and international green building professional accreditation system for draw a development plan. And then conduct an expert Delphi survey reflected in the survey results to development plan.

몰수평판에 의한 폰툰형 부유체에 작용하는 파랑강제력의 감소현상에 관한 연구

이상민,이원우,Lee, Sang-Min,Lee, Won-Woo 해양환경안전학회 2007 해양환경안전학회지 Vol.13 No.3

Pontoon type very large floating structure has been considered and actively studied as one of the most important ocean space utilization. The hydroelastic displacement of the pontoon type floating structure in waves is the largest at its weather side. The purpose of this study is to investigate the characteristics and effects of the submerged horizontal plate which is developed to reduce the wave exciting forces acting on the pontoon type floating structure using numerical analysis. The numerical method based on the finite difference method has been adopted and compared with the experimental data to confirm the reliability of it. We have performed the numerical computation of wave exciting forces acting on the pontoon type floating structure with/without submerged plate and discuss the results of simulation.

기존학교 시설개선사업의 통합적 추진을 통한 효율화 방안 -K-TEGS 평가기법을 통한-

이상민,김성중,맹준호,Lee, Sang-Min,Kim, Seong-Joong,Meang, Joon-Ho 한국교육녹색환경연구원 2011 교육·녹색환경연구 Vol.10 No.1

The importance of education and training facilities and education facilities on the relationship between effective as demonstrated in many studies can be very big. In this study, the status of existing school facilities improvement project is done individually, based on the systematic integration of the improvement project was to evaluate the feasibility. Furthermore, it was based on looking for ways to effectively pursue. Training School Facilities Improvement Program to be competitive, the country continued to be pursued systematically and very large scale as a whole must be a great national project. So much to promote efficient business environment, quality of education for children with detractors of the national budget to ensure efficient and most importantly in terms of economic investment that can be. In this study, the proposed K-TEGS existing school facilities, such as the development of techniques for the assessment based on this effort to promote an efficient business should be done on a continuous basis.

파워 분배망을 고려한 디지털 회로 시스템의 설계와 분석

이상민,문규,위재경,Lee, Sang-Min,Moon, Gyu,Wee, Jae-Kyung 대한전자공학회 2004 電子工學會論文誌-SD (Semiconductor and devices) Vol.41 No.4

This paper presents the channel analysis considering power distribution network(PDN) system of PCB. For achieve the target PDN system we proposed the useful design approach for acquiring the characteristic target of power distribution network in overall frequency ranges. The proposed method is based on the hierarchical approach related to frequency ranges and the path-based equivalent circuit model to consider the interference of the current paths between the decoupling capacitors and the board through it is a lumped model for fast and easy calculation, experimental results show that the proposed model is almost as precise as the numerical analysis. The analysis of PDN system shows that although the effective inductance of package dominatly affects the power noise and the signal transfer through data channel, the board PDNs also can not be neglected for achieving the accurate channel signaling. Therefore, we must design concurrently the chip, package, and board from the initial spec design of high speed digital system. 이 논문은 PCB의 PDN(Power Distribution Network) 시스템을 고려한 채널 분석을 나타내었다. 설계자가 원하는 PDN 시스템을 설계하기 위하여, 전체 주파수 범위의 PDN이 요구하는 임피던스를 얻는 유용한 설계방법을 제안하였다. 제안된 방법은 주파수 영역과 관계된 계층적 배치 접관방식과 보트와 decoupling 커패시터 사이의 current 흐름의 간섭을 고려한 path-based equivalent 회로를 기본으로 하였다. 비록 빠르고 쉬운 계산을 위한 lumped model일지라도, 실험 결과는 제안된 모델이 numerical 분석처럼 거의 정확함을 보였다. PDN 시스텐의 분석은 패키지 인덕턴스가 파워 노이즈, 데이터 채널을 통한 신호 이동에 영향을 받는다는 것을 보여주고 있으나, 보드 PDN 또한 정확한 채널 신호를 위해 무시할 수 없다는 것을 보여준다. 따라서 설계자는 반드시 초고속 디지털 시스템의 첫 스팩 설계로부터 보드, 패키지, 칩 등을 동시에 디자인을 해야 한다.

폐 활성슬러지의 화학적 개질을 통한 비소의 생물흡착 특성

이상민,김동욱,Lee, Sang-Min,Kim, Dong-Wook 대한자원환경지질학회 2005 자원환경지질 Vol.38 No.5

Cheap and environmental sound biosorbent was made for the adsorption of arsenate using an waste activated sludge. The biosorbents were methylated in 9hours and 24 hours respectively for the better adsorption of arsenate. The amount adsorbed of arsenate(V) increased with increasing methylation time. The specific arsenate adsorption was 0.06mmol As(V)/g biomass when the biosorbent was methylated in 24 hours. The methylated biosorbents were also studied with pH 5, 7 and 9. The pH of the solution affect the amount of adsorption of arsenate of the biosorbent even though it was methylated. The specific arsenate adsorption of the biosorbent at pH 5 was best and it was three times greater than the amount of arsenate adsorbed at pH 9.