인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

녹비작물을 이용한 윤작이 잡초 제어에 미치는 영향

이상민,이병모,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Byung-Mo,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.2

본 시험은 유기농업 파 및 고추재배를 위하여 호밀과 헤어리베치를 겨울에 윤작하여 잡초 발생량 억제에 미치는 효과를 검토하기 위하여 수행되었다. 파 재배구에서 호밀을 토양에 환원하지 않은 처리구에 비해 호밀을 환원하였을 경우 잡초발생량을 52% 경감시켰다. 고추 재배구에서는 그러한 효과가 뚜렷하게 관찰되지 않았다. 파 재배시 어저귀, 참방동사니, 바랭이와 같은 초종은 호밀뿌리 분비물에 의해 발아가 억제되는 경향이었으나 헤어리베치는 그러한 발생억제 효과가 크지 않았다. 파 재배시 호밀 환원은 잡초발생량을 경감시켰고 잡초의 종 다양성 지수를 증가시켰다. This study was conducted to evaluate the effects of crop rotation on the weed occurrence in green onion and red pepper cultivation. Rye and hairy vetch were annually seeded for winter season. The reduction of weed germination was pronounced by about 50% in green onion field, but was not significant in red pepper field through rye cropping. Among weed species, the germination of Abutilon theophrasti, Cyperus iria and Digitaria ciliaris was distinctly inhibited by exudate of rye root, but not by that of hairy vetch in vitro experiment. The return of rye biomass into onion field decreased amount of weed biomass but increased index of weed diversity.

헤어리베치(Vice villosa Roth) 품종별 국내 월동성, Biomass 및 무기태 질소 함량비교

이상민,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

헤어리베치 8종의 품종별 국내 적응성 검증을 위해 월동률과 biomass, 그리고 토양 중 무기태질소를 조사하였다. 겨울철 월동 후 생존율은 Hungvillosa와 Ostsaat 품종에서 좋았고, Minnie, Oregon common과 TTF1에서 낮았다. 생존율은 biomass에도 영향을 주어서 Hungvillosa와 Ostsaat 품종에서 가장 높았다. 헤어리베치 품종 간 무기성분 농도는 별다른 차이가 관찰되지 않았다. 토양 중 무기태질소 농도는 질소환원능이 좋았던, Hungvillosa와 Ostsaat가 예초 후 10여일 후에 크게 증가시켰다. This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

주성분 분석 기법을 이용한 심음 인식

이상민,홍승홍,Lee, Sang-Min,Hong, Seung-Hong 대한전자공학회 2001 電子工學會論文誌-SC (System and control) Vol.38 No.5

Recently many researches concerning heart sound analysis are being processed with development of digital signal processing and electronic components. But there are few researches about recognition of heart sound, especially full cardiac cycled heart sound, In this paper, a new recognition methods about. full cardiac cycled heart sound was proposed. For the first, the database was built by principal components analysis on training heart sound set. This database is used to recognize new input of heart sound, Ilear sounds were classified into seven classes such as normal(NO) class, pre-systolic murmurr(PS) class, early systolic murmur(ES) class, late systolic murmurr(LS) class, early diastolic murmur(EI) class, late diastolic murmur(LD) class and continuous murmuru(CM) class. As a result, we could verify that our new method has better efficiencies for the recognition the characteristics of heart sound than any precedent research. The maximum recognition rates of the new method are 71% for NO, 80% for PS and ES, 78% for LS, 87% for ED, 60% for LD and 20% for CM. Although the present results aren't practically sufficient to use our new method in recognizing heart sound, the importance of this paper is for recognition of heart sound within full cardiac cycle. We can get a better result by building a more efficient database. 최근에 디지털 선호처리와 전자부품의 발달로 심음 분식에 관한 많은 연구가 진행되고 있다. 그러나 심음 인식, 특히 심음 한주기 전체에 대한 인식연구는 거의 없다. 본 논문에서 심음 전체 한주기에 대한 새로운 인식 방법을 제안하였다. 먼저 주성분 분석을 이용하여 훈련 셋트로 데이터베이스를 구축한다. 데이터베이스는 새로운 심음입력을 인식하는데 이용된다. 심음은 정상심음, 수축전 심잡음, 수축초기 심잡음, 수축 말기 심잡음, 이완 초기 심잡음, 이완 말기 심잡음, 연속적 심잡음으로 분류된다. 실험결과 새로운 인식 방법은 심음의 특징을 인식하는데 효과적이었다. 최대 인식률은 NO의 경우 71%, PS와 ES의 경우 80%, LS의 경우 78%, ED의 경우 87%, LD의 경우 60%, CM의 경우 20% 이었다. 현재의 결과가 실제적으로 심음을 인식하기에는 충분하지 못하였지만 선음 전체 주기를 대상으로 한 연구라는데 의의가 있으며 더 효과적인 데이터베이스를 구축함으로써 인식률을 개선할 수 있다.

소화기질환(消化器疾患)에 사용(使用)된 약주요법(藥酒療法)에 대한 문헌적(文獻的) 고찰(考察)

이상민,강재춘,Lee Sang-Min,Kang Jae-Chun 대한예방한의학회 1999 대한예방한의학회지 Vol.3 No.1

To investigate the documental records of the medi-alcohol theraphy on the Gastrointestinal disease, the 33 kinds of medical books editioned from the old to the present were used. the results were followed Many herbs used on the Gastrointestinal disease were sequently Atractylodis Macrocephalae Rhizoma, Zingiberis Rhizoma, Ginseng Radix. The herbs of invigorating and warming body, regulating the flow of qi etc. were many occupied(used) in treating the Gastrointestinal disease. In analyzing treatment symptom as medi-alcohol theraphy were sequently many used abdominal pain, inappetence, gastrointestinal cancer, indigestion, fatigue, vomiting, diarrhea, abdominal dropsy. The kinds of using medi-alcohol on the Gastrointestinal disease were totally 120 ones.

교통조사의 개선방향에 관한 제언

이상민,추상호,Lee, Sang-Min,Chu, Sang-Ho 대한교통학회 2005 교통 기술과 정책 Vol.2 No.4

소양인(少陽人) 임신오조(姙娠惡阻) 환자를 형방사백산(荊防瀉白散)으로 치료한 치험례

이상민,배영춘,유관석,주종천,서은희,Lee, Sang-Min,Bae, Young-Chun,Yoo, Gwan-Seok,Joo, Jong-Cheon,Seo, Eun-Hee 사상체질의학회 2004 사상체질의학회지 Vol.16 No.2

1. Objectives Hyperemesis Gravidarum is one of common symptoms clinically and caused by pregnancy. This is defined as vomiting sufficiently pernicious to produce weight loss, dehydration, acid-base imbalance. In severe case, it also leads to neurologic abnormality including confusion, gate disturbance, nystagmus. We have tried researching effect of herbal medicines, Soyangin Hyungbangsabaek-san. 2. Methods To heal the Hyperemesis Gravidarum, we used Soyangin Hyungbangsabaek-san. 3. Results Hyperemesis Gravidarum of a Soyangin patient was treated with a herb-medicine, and change of her subjective symptom & general condition was managed. 4. Conclusions The constitutional treatment with herb-medicine (Soyangin Hyungbangsabaek-san) was efficacious against Hyperemesis Gravidarum. Further study on management of this disease is needed.

원형정자세포의 주입에 의한 임신과 분만 1례

이상민,정지영,한용태,박해택,박흠대,정길생,이기영,Lee, S.M.,Jung, J.Y.,Han, Y.T.,Park, H.T.,Park, H.D.,Chung, K.S.,Lee, K.Y. 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.2

We demonstrated that the normal pregnancy and delivery by round spermatid injection (ROSI) into oocytes was achieved from nonobstructive azoospermia patient. In this case, the normal fertilization rate was 50%. All of the two pronuclear stage embryos cleaved and were transferred to the patient's uterus. A singleton pregnancy was achieved and resulted in the birth of normal female infant. This resuJt show that intracytoplasraic injection of round spermatid seems to be new treatment of nonobstmctive azoospermia male infertility. Further research is needed to evaluate the required culture conditions to induce progression of the round spermatid into a more elongated stage.

레이저 범프와 대기압 변화에 대한 하드디스크 슬라이더의 부상 특성

이상민,김대은,Lee, Sang-Min,Kim, Dae-Eun 대한기계학회 2000 大韓機械學會論文集A Vol.24 No.10

The performance of slider of a hard disk drive affects the durability of the system. Particularly, the flying ability of the slider is critical in terms of surface damage and head crash. In this work, the take-off characteristics of the slider for various types of laser zone textured bump geometries were investigated. Also, the effect of ambient pressure on the flying characteristics of the slider was experimentally observed. An index of air density which can be used as a parameter for evaluating the flying characteristic is introduced.

기술이전 이후 연구자의 사업화 지원이 사업화 성공에 미치는 영향: ETRI의 사례

이상민,조근태,Lee, Sangmin,Cho, Keuntae 기술경영경제학회 2017 Journal of Technology Innovation Vol.25 No.2

본 연구는 공공연구기관의 기술이전시 연구자가 이전기술의 사업화 과정에 참여하여 이전된 기술의 발명 과정에서 습득한 노하우를 전수하는 것이 사업화 성공에 미치는 영향을 분석하였다. ETRI(Electronics and Telecommunications Research Institute)의 2014년 기술 이전 계약건중 설문조사에 응답한 204건을 분석대상으로 하였으며, 종속변수가 범주형 변수일 때 특정 사건의 발생(성공 또는 미성공) 가능성을 예측하는 통계기법인 로지스틱 회귀분석을 이용하였다. 실증분석 결과, 기술이전이후 사업화 지원의 강도가 이전된 기술의 사업화 성공여부와 양의 상관관계가 있는 것으로 나타났다. 본 연구는 공공연구기관중 최대 규모의 기술이전 및 사업화지원 실적을 보유하고 있는 ETRI의 사례를 통한 실증분석 결과라는데 의의가 있으며, 이를 통해 정부 및 공공연구기관의 중소기업 지원 정책 방향에 대한 시사점을 제시한다. The objective of the study is to examine the impact of researcher's commercialization support on the success of commercialization after technology transfer of public research institutes. Logistic regression analysis was used to analyze the effect of researcher's commercialization support on commercialization success with 204 license agreements from ETRI. As a result, it has been empirically verified that there is a significant impact to the success of the commercialization of transferred technology when the researcher is participating in the commercialization process and transfers the latent knowledge gained in the invention process to the licensed company. The result is suggestive of the effective directions of government's and public research institutes' policy for SME support.