Ulmus davidiana ethanol extract inhibits monocyte adhesion to tumor necrosis factor-alpha-stimulated endothelial cells

이기모,주희경,이유란,박명수,강건,최성아,전병화 한국한의학연구원 2016 Integrative Medicine Research Vol.5 No.2

Background Ulmus davidiana var. japonica Rehder (UD) has long been used in traditional folk medicine in Asia. This study is designed to investigate the antiadhesive activity of the ethanol extract of UD (UDE) and its underlying mechanisms in cultured endothelial cells. Methods The dried root bark of UD was extracted with 80% (v/v) ethanol. The antiadhesive activity of the UDE was investigated in cultured human umbilical vein endothelial cells and human embryonic kidney epithelial 293T (HEK 293T) cells stably transfected with pGL3-vascular cell adhesion molecule (VCAM)-1-luc. Monocyte adhesion in endothelial cells was induced by tumor necrosis factor-alpha (TNF-α), and the protective effects of UDE on monocyte–endothelial cell adhesion, VCAM-1 expression, reactive oxygen species production, and nuclear factor-κB activity were determined. Results Exposure to UDE at a concentration of 3–30 μg/mL for 24 hours produced no detectable cytotoxicity in human umbilical vein endothelial cells, but it significantly inhibited TNF-α-induced monocyte adhesion and VCAM-1 expression. TNF-α treatment of HEK 293T/VCAM-1-luc cells resulted in increased luciferase activity of the VCAM-1 promoter, which was inhibited by treatment with UDE. Additionally, TNF-α-induced reactive oxygen species generation, nuclear translocation of nuclear factor-κB, and IκBα degradation in human umbilical vein endothelial cells were effectively reduced by treatment with 30 μg/mL of UDE. Conclusion Our results indicated that UDE treatment inhibited TNF-α-induced monocyte adhesion in endothelial cells, suggesting that UD may reduce vascular endothelial inflammation.

Screening of Preventive Effects of Herbal Prescriptions in Rat Model of Arterial Thrombosis Induced by Ferric Chloride

이기모,방지혜,이인선,허은정,김부여,강형원,류영수,전원경 대한한의학회 2013 대한한의학회지 Vol.34 No.2

Objectives: This study examined the effects of 8 types of herbal prescriptions prescribed to alleviate dementia symptoms in a rat model of arterial thrombosis induced by ferric chloride (FeCl3). Methods: Thirty minutes before 35% FeCl3 treatment, SD rats were intraperitoneally injected with the 8 types of herbal prescriptions, respectively. We tested the effects of the herbal prescriptions on time to occlusion (TTO) in an arterial thrombosis model using a laser Doppler flow meter. In addition, thrombus weight (TW) and collagen fiber damages were evaluated in the same condition. Results: Herbal prescriptions showed the following rank-order based on their TTO: Chong-myung-tang (CMT) > modified Jangwonhan 02 (LMK02) > Toki-shakuyaku-san (TSS) ≥ Oren-gedoku-to (OGT) ≥ Yokukansan (YKS). In particular, CMT (100 mg/kg, i.p.) and LMK02 (100 mg/kg, i.p.) delayed the TTO the most (14.83 ± 0.98 and 13.67 ± 1.03 min, respectively) compared with the vehicle group (7.95 ± 0.78 min, P<0.001). In addition, CMT, LMK02, and OGT treatment (0.63 ± 0.01, 0.66 ± 0.02 and 0.67 ± 0.01 mg/mm, respectively) significantly reduced thrombus weight compared with the vehicle treatment (0.78 ± 0.03, P<0.001) and also alleviated collagen fiber damage (CMT; 28.40 ± 2.22%, LMK02; 30.79 ± 4.07%, OGT; 26.20 ± 1.48%) in the vessels injured by FeCl3. Therefore, CMT and LMK02 showed the greatest preventive activity in rat model of arterial thrombosis induced by FeCl3. Conclusions: These results provide experimental evidence for traditional use of herbal prescriptions, suggesting that CMT and LMK02 extracts could be used to prevent vascular injury and thrombosis in the early stages of dementia.

분자동역학을 이용한 플리머의 마찰계수 측정

이기모,문성남,이우일 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.5-4

Effect of the anisotropic surface geometry on the water droplet shape

문성남,이기모,정재민,이우일 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.5

Anti-thrombotic Effects of Modified Jeho-tang using a FeCl3-induced Carotid Arterial Thrombosis Model

방지혜,이기모,김부여,이정화,이인선,전원경 대한한의학회 2013 대한한의학회지 Vol.34 No.2

Objectives: The aim of this study was to examine the antithrombotic effects of the four herbal ingredients (Mume Fructus, MF; Santali Albi Lignum, SAL; Amomi Tsao-Ko Fructus, ATF; and Amomi Fructus, AF) of modified Jeho-tang (MJHT) in a ferric chloride (FeCl3)-induced carotid arterial thrombosis model. Methods: Thirty minutes prior to a 35% FeCl3 application, Sprague-Dawley (SD) rats were injected with saline, MF, SAL, ATF or AF (100 mg/kg, intraperitoneal injection), respectively. The effect of the MJHT ingredients was examined for time to occlusion (TTO) and thrombus weight (TW) in a FeCl3-induced thrombosis model. Histological analysis was performed to examine the effect of the MJHT ingredients on collagen fiber damage using hematoxylin & eosin and Masson’s trichrome staining. Results: Compared with vehicle treatment, MF, SAL and ATF treatment delayed TTO (vehicle, 8.11 ± 0.60 min; MF, 16.67 ± 1.03 min; SAL, 17.50 ± 1.52 min and ATF, 13.33 ± 1.21 min; P < 0.001) and inhibited thrombus formation (vehicle, 0.79 ± 0.03 mg/mm; MF, 0.61 ± 0.07 mg/mm; SAL, 0.57 ± 0.03 mg/mm and ATF, 0.72 ± 0.02 mg/mm; P < 0.001). In addition, each herbal ingredient of MJHT except for AF prevented the collagen fiber damage induced by a 35% FeCl3 application. These results indicate that the MJHT ingredients MF ≥ SAL 〉ATF 〉AF possess antithrombotic activity in a FeCl3-induced carotid arterial thrombosis. Conclusions: Altogether, these results are the first evidence that the MJHT ingredients MF, SAL and ATF have the ability to prevent vascular damage and thrombus formation in FeCl3-induced carotid arterial thrombosis.

Effect of the Radius of Gyration on the Polymer Nano-Patterning

문성남,이동언,정재민,이기모,이우일 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.5-4

경동맥 혈전 유발 동물모델을 이용한 한약재 추출물의 효능 검색

박인실 ( In Sil Park ),이기모 ( Ki Mo Lee ),이인선 ( In Sun Lee ),한재일 ( Jae Il Han ),전원경 ( Won Kyung Jeon ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.5

Objectives: The aim of this study was to examine anti-thrombotic effect of traditional herbal extracts in a rat model of ferric chloride (FeCl3)-induced carotid arterial thrombosis. Methods: Thirty minutes prior to a 35% FeCl3 application, Sprague Dawley(SD) rats were injected with the 10 types of traditional herbal extracts (100mg/kg, intraperitoneal injection), respectively. The effect of these herbal extracts was examined for time to occlusion(TTO) using the Laser doppler flow meter and measured for thrombus weight (TW) in FeCl3-induced thrombosis model. Results: In the TTO, Salvia miltiorrhiza (Sm, 2.30±0.28 min, p<0.001) and Santalum album (Sa, 2.19±0.19 min, p<0.001) showed significantly delayed TTO more than twice compared with Saline-treated group. Cnidium officinale (Co), Psoralea corylifolia (Pc), Scutellatia baicalensis (Sba), Panax notoginseng (Pn), Angelica tenuissima (At), Scrophularia buergeriana (Sbu), Rhus verniciflua (Rv) and Picrasma quassioides (Pq), except for Rhus verniciflua (Rv) also meaningfully impeded TTO more than one fold. In addition, Salvia miltiorrhiza, Santalum album, Cnidium officinale, Psoralea corylifolia and Scutellatia baicalensis significantly reduced TW more than 10% compared with Saline-treated group. Especially, Salvia miltiorrhiza and Santalum album showed the most excellent anti-thrombotic effect among the 10 herbal extracts tested on the restoration of altered TTO and TW. Conclusions: These results suggest that Sm and Sa extracts have outstanding anti-thrombotic effect in FeCl3-induced thrombosis model and is potentially useful as herbal medicines for the treatment and prevention of thrombosis.

Evaluation of the Effects of Acorus gramineus and Acorus tatarinowii Extracts on a Rat Model of Arterial Thrombosis induced by Ferric Chloride

Jihye Bang,전원경,이기모,Eun-Jung Heo,강형원,In Sun Lee 한국생약학회 2013 Natural Product Sciences Vol.19 No.3

We investigated the antithrombotic effect of Acorus gramineus Soland (A. gramineus) from Korea and Acorus tatarinowii Schott (A. tatarinowii) from China in a rat model of arterial thrombosis induced by ferric chloride (FeCl3). Thirty minutes prior to a 35% FeCl3 application, Sprague Dawley rats were intraperitoneally injected with saline, A. gramineus and A. tatarinowii (100 mg/kg), respectively. Occlusion time of rats injected with A. gramineus was delayed significantly compared to that of the vehicle and A. tatarinowii. Thrombus weight was meaningfully decreased in rats injected with A. gramineus compared to the vehicle. Additionally, A. gramineus inhibited collagen fiber damage in vessel compared to the vehicle, but A. tatarinowii did not show a significant effect. Our results show that A. gramineus and A. tatarinowii from the same genus have different antithrombotic effects, and especially A. gramineus has a better antithrombotic effect than A. tatarinowii.

Salicornia herbacea Aqueous Extracts Regulate NLRP3 Inflammasome Activation in Macrophages and Trophoblasts

Eui-Jeong Noh,Jun-Young Lee,Seo-Ye Park,Jong-Hwan Park,Jeong-Yong Cho,Young Min Kim,Jong-Seok Kim,이기모,Sunga Choi,Sung Ki Lee 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.5

Salicornia herbacea L. (Chenopodiaceae), an edible salt marsh plant with anti-inflammatory effects, was examined in macrophages and trophoblasts whether it modulates NLRP3 inflammasome activity. Pretreatment and delayed treatment of S. herbacea extract (SHE) in bone marrow-derived macrophages (BMDMs) reduced the activity of NLRP3 inflammasome induced by lipopolysaccharide (LPS) and adenosine triphosphate stimulation and downregulated interleukin (IL)-1β production. SHE also inhibited pyroptotic cell death, the adaptor molecule apoptosis-associated speck-like protein containing a CARD (ASC), oligomerization, and speck by NLRP3 inflammasome activity in BMDM. Similarly, SHE decreased the mRNA expression of NLRP3, ASC, IL-1β, and IL-6 in the LPS-stimulated human trophoblast cell line, Swan 71 cells. In addition, SHE inhibited the production of IL-6 and IL-1β and decreased the expression of cyclooxygenase-2 and prostaglandin E2 in stimulated Swan 71 cells. Finally, 3,5-dicaffeoylquinic acid (3,5-DCQA), one of the components of S. herbacea, inhibited IL-1β produced by NLRP3 inflammasome activity. In conclusion, SHE downregulated the activity of the NLRP3 inflammasome in macrophages and trophoblasts.