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엄석현,박형재,김성무,박승문,김명조,유창연,조동하 韓國藥用作物學會 2007 한국약용작물학회지 Vol.15 No.4
가래나무 잎을 원적외선 30, 40 및 50 v에서 각각 10, 20 및 30분을 처리하였다. 원적외선을 조사한 잎의 총 폴리페놀 함량, 총 플라보노이드 함량, 잎 추출물의 DPPH radical 소거 활성 등을 분석한 결과, 원적외선 30 v에서 20분간 조사한 처리구에서 총 폴리페놀 함량은 25.43 mg/g로 , 총 플라보노이드 함량은 39.38 mg/g으로 가장 높게 나타났다. 그 반면에, 원적외선 50 v에서 30분간 조사한 처리구에서 총폴리페놀 함량은 16.93 mg/g으로, 총플라보노이드 함량은 23.78 mg/g으로 가장 낮게 나타났다. 전체적으로 원적외선 처리에 의해 잎에 함유한 항산화 물질의 함량의 증가는 물론, 그 추출물의 항산화 활성도 증가하는 것을 알 수 있었다. 하지만 원적외선 처리가 가래나무에 함유한 어떠한 특정적인 물질들의 증가에 관하여서는 추후에 더욱 많은 연구가 필요할 것으로 사료된다. This research was conducted to investigate antioxidant activities of Juglans mandshrica forages by the irradiation of far infrared ray during leaf drying processes before compound extraction, with its potential use for activating antioxidants. The contents of total phenolics and flavonoids in the basis of a gram leaf dry weight were maximized in 20 min at 30 V of far infrared irradiation. Total phenolic content was 25.43 mg/g in the FIR treatment, while it was 21.12 mg/g in the non-FIR treatment control. Total flavonoid content was 39.38 mg/g in the FIR treatment, while it was 32.78 mg/g in the non-FIR treatment control. The contents were decreased when the condition of far infrared ray irradiation was higher voltage and longer treatment time, exhibiting 16.93 mg/g of total phenolics and 23.78 mg/g of total flavonoids in 30 min at 50 V of far infrared irradiation. The antioxidant activities were shown to positive relationships with the contents of total phenolics and total flavonoids. In this study, we concluded that content of bioactive molecules relating antioxidant activity in Juglans mandshrica forages is optimized in 20 min at 30 V of far infrared irradiation for leaf drying process.
엄석현 ( Seok Hyeon Eom ),박창환 ( Chang Hwan Park ),정덕원 ( Duk Won Chung ),이상혁 ( Sang Hyeok Lee ),서지영 ( Ji Young Seo ),김영성 ( Yeong Sung Kim ),곽동협 ( Dong Hyup Kwak ),김정희 ( Jung Hee Kim ) 영남대학교 의과대학 2016 Yeungnam University Journal of Medicine Vol.33 No.2
Heterotopic bone formation in the gastrointestinal tract is a rare phenomenon. Most reported cases were associated with benign and malignant neoplasms, except for a case in which heterotopic bone formation was found in a patient with Barrett`s esophagus. The exact pathogenesis of the disease has not yet been established. However, most heterotopic bones found in the gastrointestinal tract were associated with mucinproducing tumors of the appendix, colon, and rectum. Inflammation may also play a role in osseous metaplasia in a case with bone formation at the base of an ulcer in Barrett`s esophagus. Here, we report on a patient with heterotopic bone formation in normal gastric cardiac mucosa. A 50-year-old female visited our hospital for a routine health examination. She had no gastrointestinal symptoms, and her physical examination, blood test, X-ray, urine, and stool examination results were normal. A 0.3 cm sized polypoid lesion located just below the squamocolumnar junction was observed on upper gastrointestinal endoscopy. A piece of biopsy was taken. Histologically, a lamella bone trabecula and chronic inflammatory cells were observed in the gastric cardiac mucosa. The follow-up endoscopy performed one month later showed no residual lesion.
Assessment on Antioxidant Properties of Oplopanax elatus Nakai in vitro
김준형,엄석현,이한신,김재광,유창연,권용수,이주경,김명조 한국약용작물학회 2007 韓國藥用作物學會誌 Vol.15 No.2
Effects of Oplopanax elatus have known to various pharmaceutical therapies. However, chemical prosperities inthe plant are rarely investigated. In order to detect biological activity, we evaluated the antioxidant activity of five fractionsfrom methanolic extracts in each part of O. elatus. Also, contents of polyphenols and polysaccharides were measured. Five fractionswere of sub-fractions using n-hexane, ethyl-acetate, n-butanol, and water from methanolic extracts. Ethylacetate and nbutanolfractions from stem and root exhibited strong antioxidant activity and high total phenolics content. On the HPLCanalysis, ten free phenolics, including p-hydroxybenzoic acid, chlorogenic acid, caffeic acid, syringic acid, p-coumaric acid, ferulicacid, naringin, hesperidin, quercetin and trans-cinnamic acid, were identified from the fractions and were shown to differentquantitative proportions. Furthermore, ethylacetate and n-butanol fraction had the highest amount of chlorogenic acids,one of the cinnamic acid derivatives possessing pharmacological properties. These results indicated that the fractions of O. elatus,as well as methanolic extracts, could be used as natural antioxidative ingredients. Effects of Oplopanax elatus have known to various pharmaceutical therapies. However, chemical prosperities in the plant are rarely investigated. In order to detect biological activity, we evaluated the antioxidant activity of five fractions from methanolic extracts in each part of O. elatus. Also, contents of polyphenols and polysaccharides were measured. Five fractions were of sub-fractions using n-hexane, ethyl-acetate, n-butanol, and water from methanolic extracts. Ethylacetate and nbutanol fractions from stem and root exhibited strong antioxidant activity and high total phenolics content. On the HPLC analysis, ten free phenolics, including p-hydroxybenzoic acid, chlorogenic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, naringin, hesperidin, quercetin and trans-cinnamic acid, were identified from the fractions and were shown to different quantitative proportions. Furthermore, ethylacetate and n-butanol fraction had the highest amount of chlorogenic acids, one of the cinnamic acid derivatives possessing pharmacological properties. These results indicated that the fractions of O. elatus, as well as methanolic extracts, could be used as natural antioxidative ingredients.
홍보경,엄석현,이찬옥,이지원,정종현,김재광,조동하,유창연,권용수,김명조 한국약용작물학회 2007 한국약용작물학회지 Vol.15 No.4
본 연구에서는 소종과 외상출혈 치료에 사용되며, 주로 간암, 간경화 등의 간 질환 치료제로 사용되어 온 산겨릅나무 추출물에서 식품 및 음료 등과 같은 기능성 식품으로서의 다양한 산업적 응용분야에 널리 응용될 수 있는 기초연구의 일환으로 산겨릅나무 추출물을 이용하여 항산화 활성, 항지질과 산화 활성, 항미생물 활성, 항보체 활성을 실험하였으며, 또한 유용한 생리활성 성분을 분리, 동정하고자 하였다. 그 결과 산겨릅나무 추출물은 DPPH free radical 소거법에 의한 항산화 활성 실험에서는 ehtyl acetate 분획 (RC50= 3.15 μg/ml), butanol 분획 (RC50= 5.17 μg/ml)에서 강한 항산화 활성을 보였다. 또한 분리된 2개의 화합물도 대조군인 α-tocopherol 이나 BHA보다 유사하거나 강한 항산화 활성을 나타냈다. linoleic acid에 대한 항지질과산화 활성 실험은 15일 째에 물층을 제외한 추출물, 분획물 및 화합물에서 높은 활성을 나타냈으며 특히 화합물 2 (85%)는 항산화 물질로 알려진 catechin (85%)과 유사한 활성을 나타내었다. 박테리아에 대한 향균실험은 Staphylococus aureus 에서만 활성을 보이지 않았을 뿐 다른 피검균에서는 높은 활성을 보였다. 하지만 대부분의 피검균에 대하여 강한 활성을 보인 ethyl acetate 분획에서 분리된 화합물 1, 2는 모든 피검균에 대해 활성을 보이지 않았다. 다만 fungal strain인 Candida albicands에 대해 각각 250 μg/ml. 500 μg/ml의 생육 억제 농도를 나타내었다. 보체계 활성화능을 측정한 결과 물 층 24%를 제외한 다른 분획물은 10% 이하의 낮은 억제효과를 보이거나 활성이 나타나지 않았다. 산겨릅나무 ethyl acetate 분획을 silica gel이 충전된 grass open column에 넣은 후, toluene, acetone, methanol의 혼합용매로 순차용출 (stepwise)시킨 후, ODS와 silica gel을 이용하여 column chromatography를 실시함으로서 2종의 단일 물질을 얻었다. 이 화합물은 lH- 및 13C-NMR, IR, UV, EI/FAB-Mass spectrum을 이용하여 기기분석한 결과 compound 1은 catechin으로, compound 2는 σ-Hydroxyphenethyl alcohol 1-O-β-D-(6'-O-galloyl)-glucopyranoside로 동정되었으며, 특히 compound 2의 경우 산겨릅나무에서 처음 분리되었다. 위 결과 산겨릅나무 추출물은 항산화 활성, 항지질과산화 활성, 항미생물 활성이 우수하였다. 또한 산겨릅나무 추출물을 이용한다면 식품 및 음료 등과 같은 기능성 식품으로서의 다양한 산업적 응용분야에 널리 응용될 수 있으리라 사례된다. Acer tegmentosum (Acereaceae) has been used a source of traditional medicines for the treatment of hepatic disorders in Korea. This research was conducted to determine biofunctional activities of A. tegmentosum stem extract and to identify its bioactive components. Methanolic extract from A. tegmentosum stem was partitioned by using organic solvents, including n-hexane, ethyl acetate, n-butanol, and water. Two compounds were isolated by using an ODS column chromatography from ethyl acetate soluble fraction shown to the strongest antioxidant activity (RC50=3.15 μg/ml) among the fractions. The isolated compounds were analyzed by 1H and 13C NMR, IR, UV/VIS, MS spectrum data and identified as catechin, σ-Hydroxyphenethyl alcohol 1-O-β-D-(6'-O-galloyl)-glucopyranoside. The compounds have shown strong antioxidant activity, with similar activity to BHA (RC50=2 μg/ml). Especially, σ-Hydroxyphenethyl alcohol 1-O-β-D-(6'-O-galloyl)-glucopyranoside was shown strong anti-lipid peroxidative activity. However, the compounds were not shown antimicrobial activities. In antimicrobial activity assays, ethyl acetate soluble fraction was effective to bacterial inhibition, such as Escherichia coli and Klebsiella pneumonia, with minimum inhibitory concentrations in 125 μg/ml. Otherwise, antifungal activity against Candida albicans was shown in n-hexane soluble fraction exhibiting 63 μg/ml of minimum inhibitory concentration. In anticomplementary activity assays, water soluble fraction was the most effective exhibiting 24% inhibitory activity.