Extracellular signal regulated kinases in the spinal cord of rats with experimental autoimmune encephalomyelitis

안미정,허승담,지영흔,주홍구,이용덕,심기범,신태균,Ahn, Mee-jung,Heo, Seung-dam,Jee, Young-heun,Joo, Hong-gu,Lee, Yong-duk,Sim, Ki-Bum,Shin, Tae-kyun The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.4

The phosphorylation of extracellular signal-regulated kinases (p-ERK) in the spinal cord of rats with acute monophasic experimental autoimmune encephalomyelitis (EAE) was studied using immunohistochemistry and treatment with inhibitor. P-ERK is constitutively expressed in glial cells in the normal spinal cord. In EAE, some inflammatory cells in the subarachnoid space were positive for p-ERK at the early stage, and its immunoreactivity declined when those cells infiltrated the parenchyma at the peak stage. In a blocking experiment using its inhibitor, the intravenous administration of PD98059 from day 7 to 13 post-immunization did not modulate EAE paralysis. Considering the results, we postulate that intravenous administration of PD98059 is not effective in ameliorating EAE paralysis, although many inflammatory cells express ERK in the subarachnoid space.

제주지역 양돈장에서 발생한 자돈 설사병 바이러스의 면역조직화학적 확인

안미정 ( Mee Jung Ahn ),김진희 ( Jin Hoe Kim ),강완철 ( Wan Cheul Kang ),강종철 ( Jong Cheul Kang ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.3

In an outbreak of acute porcine diarrhea in newborn piglets, an etiological study was carried out using piglets submitted in Cheju Province Institute for Livestock Promotion(Cheju Veterinary Service for the disease diagnosis). Sixteen piglets(2-7 days old) were collected from 4 farms during outbreaks of diarrhea disease(from January to April 2000). Specimens were taken after necropsy and examined by inimunohistochemistry using of monoclonal antibodies for porcine epidemic diarrhea(PED) virus, transmissible gastroenteritis(TGE) virus, and porcine rotavirus. Immunohistochernistry showed that PED virus antigens, but both TGE virus and rota virus antigens not, were localized in the some epithelial cells of the intestines of 14 animals among 16 piglets examined. PED virus antigens were mainly detected in the cytoplasm of enterocytes. Infected cells, which were most abundant in the villous epithelial cells of the jejunum and ileum, were uncommon in the crypt, epithelial cells, the lamina propria and Peyer` s patches of piglets examined. The results suggest that PED virus is one of the most prevailing agents in an outbreak of fatal diarrhea in newborn piglets on Cheju island and PED virus was need to further study to prevent this disease.

자기면역성 뇌척수염 조직에서 CPP32의 면역조직화학적 관찰

신태균,문창종,안미정,위명복,Shin, Tae-kyun,Moon, Chang-jong,Ahn, Mee-jung,Wie, Myung-bok 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.3

The aim of this study was to evaluate the involvement of CPP32 (caspase-3), one of the death-related enzymes, in the course of experimental autoimmune encephalomyelitis (EAE). EAE was induced in Lewis rats immunized with an emulsion of rat spinal cord homogenate with complete Freunds adjuvant supplemented with Mycobacterium tuberculosis (H37Ra, 5mg/ml). The expression of CPP32 in the spinal cords of rats with EAE was studied. In normal rat spinal cords, CPP32 is constitutively, but weakly, expressed in neurons and some neuroglial cells. In the EAE spinal cords, many inflammatory cells were positive for CPP 32, and the majority of CPP32(+) cells were identified as ED1(+) macrophages. During this stage of EAE, the number of CPP32(+) cells in brain cells, including neurons and astrocytes, increased, and these cells also had increased CPP32 immunoreactivity. CPP32 immunor eactivity was not always matched with apoptosis of inflammatory cells in EAE lesions. We speculate that CPP32, which is constitutlvely expressed in brain cells, increases in response to neuroimmunological stimulation in both brain neuronal cells and inflammatory cells. The functional role of CPP32 in neuroimmunological disorders is discussed.

제주지역 양돈장 자돈에서 발생한 이유후 전신성 소모성 증후군의 증례

강종철,정경주,안미정,이두식,강완철,김진회,신태균,Kang, Jong-chul,Jeong, Kyong-ju,Ahn, Mee-jung,Lee, Du-sik,Kang, Wan-chul,Kim, Jin-hoe,Shin, Tae-kyun 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3

Multiplex PCR and immunohistochemistry were used to detect and differentiate between porcine circovirus (PCV) type-I and the PCV associated with postweaning multisystemic wasting syndrome (PMWS). Unique DNA product to PCV type-II was confirmed the some organs including lymph nodes, tonsil and spleen from eight pigs in Jeju by multiplex PCR. In this study, the samples were tested by a multiplex PCR assay to determine the type of PCV in each case; all cases were PCV type-II positive. PCV type-II was identified not only in typical PMWS cases, but also in field cases submitted with various clinical histories, some of which were not suggestive of PMWS. Immunohistochemically PCV type-II antigen was detected in macrophage-like cells in the tonsil, liver, lymph nodes and spleen, while some hepatocytes and renal tubular epithelial cells were also positive to the virus. This study suggested that PCV type-II is one of the causative agents of PMWS as well as the major type of PCV in the affected pigs in Jeju.

볏붉은잎 추출물의 돼지 유래 병원성 세균에 대한 항균효과

정진우 ( Jin Woo Jeong ),정찬우 ( Chan Woo Jeong ),김정태 ( Jeong Tae Kim ),양원준 ( Won Joon Yang ),안미정 ( Mee Jung Ahn ),김병학 ( Byeoung Hak Kim ),김주아 ( Joo Ah Kim ),신태균 ( Tae Kyun Shin ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.4

Interest in marine organisms as potential sources of bioactive agents has increased in recent years. The red seaweed, Callophyllis (C.) japonica, is abundant in the coastal regions of Jeju Island in South Korea. A previous study shows that C. japonica extracts have antioxidant activity and radioprotective effects. In this study, an methanol extract of C. japonica was tested whether it has antibacterial effects against porcine bacteria. the in vitro antibacterial activities of 80% methanol crude extracts prepared from the C. japonica were tested for inhibitory activity against the Escherichia (E.) coli (S175), Enterococcus (E.) faecium (ATCC 51558), Salmonella (S.) Typhimurium and Staphylococcus (S.) aureus (ATCC 25923) using broth dilution method. All organisms were incubated in brain heart infusion (BHI medium) containing 1% extract at 0, 4, 8, 12 and 24 hrs. Suckling piglets were fed an experimental diet supplement with 1% C. japonica for 1 week. And the change of fecal coliform bacteria were examined after supplement of C. japonica for 1 week. The inocula containing 102-103CFU/ml of each organism were used. The extracts of C. japonica were shown to be antibacterial effects on all bacteria tested. The CFU value (6.3×108CFU/ml) of C. japonica for E. coli was decreased 30% compared with vehicle controls (9.0×108CFU/ml) after 8 hrs incubation. For E. faecium was inhibited proliferation rate about 68% at 4h, 81% at 8 hrs and 76% at 12 hrs after incubations, respectively. For S. Typhimurium was inhibited proliferation rate about 96% at 4h, 90% at 8 hrs and 72% at 12h after incubation with extracts of C. japonica. For S. aureus was inhibited proliferation rate more than 90% each time courses. Conclusively, a red seaweed extract of C. japonica was found to be effective against a number of gram negative and gram positive bacteria such as E. coli, E. faecium, S. Typhimurium, and S. aureus. The number of coliform bacteria was increased in the 1% C. japonica treated group, as compared to those of controls. This result suggests that C. japonica extracts be added as an effective natural antibacterial agent. The precise mechanism of antibacterial effects and its application on swine industry remains to be further studied.

감마선 조사 랫트의 고환에서 fibronectin의 발현

정진우,김희철,김정태,안미정,이지영,신태균 제주대학교 방사능이용연구소 2005 방사선응용과학연구소논문집 Vol.19 No.-

암환자에게 제공된 다빈도 간호중재 분석 : 5개 종합병원을 중심으로

문경희,안미정,김필자,박정연,김명애,박인숙,배수현,이소정,권인각,김소선 병원간호사회 2009 임상간호연구 Vol.15 No.1

Purpose: The purpose of this study was to identify nursing interventions frequently used with cancer patients. Nursing records from 5 general hospitals for patients with stomach, liver, lung or colon cancer were analyzed. Method: A descriptive study methodology was used and nursing records for 15 patients in each disease category at each hospital, who were admitted and discharged during June 2007 were analyzed. Results: Five domains of NIC were found and the physiological(basic) domain was most frequent (31.52%). Twenty two classes of NIC were identified with risk management for safety being most frequent (22.49%). For the 119 nursing interventions identified, the most frequent was pain management with 7,827 (12.31%), followed by prevention of falls (11.76%), surveillance (6.79%) and wound care (5.12%). Nursing activities of pain management and prevention of falls were comparable to activities listed in literature on guidelines for evidence based and best practices in nursing care. Eight of the 17 nursing activities for pain management, and 9 of 14 for fall prevention were consistent with these guidelines. Conclusion: In this study, nursing interventions were found to be focused on physical care, monitoring patients' condition and education. We have to develop diverse nursing interventions and a convenient recording process.

유방 종괴 절제술을 받은 환자에서 수술후 오심 구토에 대한 Propofol 마취와 Ondansetron의 예방적 투여의 비교

최세진,김윤희,안미정,조정옥 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

Breast surgery is associated with a high incidence of postoperative nausea and vomiting. Propofol as an induction and maintenance agent has been associated with a lower incidence of postoperative nausea and vomiting. Ondansetron is a 5TH-3 antagonist and is an effective antiemetic. We conducted a randomized study to compare the efficacy of ondansetron and intraoperative propofol in the prevention of postoperative nausea and vomiting in the breast surgery. 90 ASA 1 or 2 patient between ages 18-55 scheduled for breast surgery were assigned randomly to one of three groups. In groups. In group I, anesthesia was induced with thiopental 5mg/kg, fentanyl 150mcg and maintained with enflurane 1-1.5vol%. In group P, anesthesia was induced with fentanyl 150mcg, propofol 2mg/kg and maintained with propofol infusion 0.05-0.5mg/kg/min. In group O, ondansetron 4mg was administered intransvenously before induction of anesthesia. In group O, anesthesia was induced with thiopental 5mg/kg, fentanyl 150mcg and maintained with enflurane 1-1.5vol%, In All groups, 50% o_2 and 50% N_2O was administered. All patient received vecuronium to facilitated intubation and subsequent muscle relaxation during surgery. Fentanyl i.v. was used as an analgesic during anesthesia. The incidence of nausea, vomiting and request for rescue antiemetics were recorded at PACU, 6hr, 12hr, and 24hr postoperatively. The incidence of postoperative nausea and request for rescue antiemetics in group P and group O were significantly lower group I (p<0.1). But the incidence of vomiting in groups was not significantly different(p>0.1). The Prophylatic ondansetron and popofol anesthesia reduces nausea and antiemetic requirements after breast surgery.