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        Identification and Quantification of Steroidal Saponins in Polygonatum Species by HPLC/ESI/MS

        안미정,김진웅 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.5

        An HPLC/ESI/MS method has been developed to identify and quantify the spirostanol glycosides in the rhizomes of five Polygonatum species. The relative distribution of two steroidal saponins in each extract was established using the selective ion monitoring (SIM) mode via an electrospray ionization (ESI) source. It was found that there were significant differences in the amount of spirostanol glycosides among the Polygonatum species. The results showed that this method could be used to identify the steroidal saponins in the extracts and differentiate Polygonatum species with high sensitivity and reproducibility in a short time. Fragmentation patterns of the two reference compounds were also discussed with the electrospray ionization multi-stage tandem mass spectroscopy (ESI-MSn).

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        초등 영재학생과 일반학생의 진로인식, 과학 선호도 및 과학자의 정형화된 이미지 비교

        안미정,유미현 韓國英才學會 2012 영재교육연구 Vol.22 No.3

        본 연구의 목적은 초등학교 영재학급 학생과 일반학급 학생들의 진로인식과 과학 선호도 및 과학자의 정형화된 이미지를 비교하는 것이다. 본 연구를 위해 초등학교 6학년 영재학생 52명, 일반학생 80명이 연구에 참여하였다. 본 연구의 결과는 다음과 같다. 첫째, 영재학생과 일반학생의 진로인식을 비교한 결과, 영재학생들의 진로인식이 더 높게 나왔으며, 두 집단 간의 유의미한 차이가 나타났다. 이를 통해 전반적으로 일반학생에 비해서 영재학생의 진로인식이 높다는 것을 알 수 있다. 둘째, 영재학생과 일반학급 학생의 과학 선호도는 영재학생이 유의미하게 높은 결과가 나왔다. 하위영역별로 자세히 보면 6개의 모든 하위영역에서 영재학생이 일반학생보다 유의미하게 높았으며, 성별에 따른 비교에서도 영재학생에서만 유의미한 차이가 있었다. 또한 집단과 성별의 차이 간 상호작용 효과는 일반학생에 비하여 영재학생이 과학 학습의 흥미와 학습 지속 실행의지가 높게 나타났다. 셋째, 영재학생과 일반학생의 정형화된 과학자의 이미지 검사를 실시한 결과, 영재학생에 비해 일반학생이 느끼는 과학자의 이미지에서 더 많은 정형화된 모습을 볼 수 있었다. 넷째, 영재학생의 진로인식과 과학 선호도, 진로인식과 과학자의 정형화된 이미지의 상관관계를 살펴본 결과 각각의 변인들 간에 유의미한 상관관계가 나타났다. 다중회귀분석을 통해 진로인식이 과학 선호도에 유의미하게 영향을 준다는 결과를 얻었다. The purpose of this study was to investigate the career awareness, the preference for science and stereotypic image of the scientist between the gifted students and non-gifted students in elementary school. For this study, 52 gifted students and 80 non-gifted students were participated. The results were as follows: First, the career awareness of gifted students was significantly higher than that of non-gifted students. Second, the preference for science of gifted students was significantly higher in all sub-domains than non-gifted students. There was a significant interaction effect between group and gender in the scientific interest and the intention of solving problems. Third, analyzing stereotypic image test for scientist between the gifted students and non-gifted students, it proved that non-gifted students had more stereotypic image comparing with the gifted students. Forth, carrying out the correlation analysis on the career awareness and the preference for science, the career awareness and the stereotypic image of the scientist, it proved that there were significant correlations with each other. It revealed that the career awareness affected the preference for science significantly through multiple regression analysis.

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        Black Radish (Raphanus sativus L. var. niger) Extract Mediates Its Hepatoprotective Effect on Carbon Tetrachloride-Induced Hepatic Injury by Attenuating Oxidative Stress

        안미정,김정태,홍승현,김정은,고하나,이남호,김기옥,신태균 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.9

        Nonalcoholic fatty liver disease is a serious liver disorder associated with oxidative stress. Black radish (Raphanus sativus L. var. niger) extract (BRE) can lower the risk of this disease. The hepatoprotective effect of BRE containing 3-(E)-(methylthio)methylene-2-pyrrolidinethione was evaluated in human hepatocyte carcinoma (HepG2) cells and in rat livers with carbon tetrachloride (CCl4)-induced hepatic injury. BRE was administered at 125, 250, 500, and 1000 μg/mL to the oleic acid-induced HepG2 cells. Male Sprague-Dawley rats were randomly divided into seven groups: the control group, BRE group, CCl4 group, and BRE + CCl4 group. BRE was administered orally at 125, 250, 500, and 1000 mg/kg/day once daily for 7 consecutive days, followed by a single oral treatment of 1.5 mL/kg CCl4. Inhibition of lipid accumulation, serum markers of liver injury, histological evaluations, levels of oxidative stress related enzymatic and nonenzymatic antioxidants in HepG2 cells and liver tissue were investigated. The protein expression of main liver P450 isoenzymes such as cytochrome p450(CYP)2E1, the expression of nuclear factor erythroid 2-related factor-2(Nrf-2) and heme oxygenase-1(HO-1) were also studied. BRE has an inhibitory effect on lipid accumulation and caused acute hepatotoxicity manifested by increased levels of lipid peroxidation, serum alanine aminotransferase, and aspartate aminotransferase with corresponding histopathological changes and high levels of oxidative stress. BRE treatment significantly increased the level of CYP2E1, Nrf-2, and HO-1 in a dose-dependent manner. Besides, 3-(E)-(methylthio)methylene-2-pyrrolidinethione significantly increased radical-scavenging effects and the expression of Nrf-2 in oleic acid-treated HepG2 cells. These results suggest that BRE treatment reduces lipid accumulation in oleic acid-induced steatosis of HepG2 cells, and has a hepatoprotective effect against CCl4-induced liver injury in rats, possibly through Nrf-2/HO-1-mediated antioxidant effects.

      • KCI등재

        부산 사할린 영주귀국자의 이주와 가족

        안미정 부경역사연구소 2014 지역과 역사 Vol.- No.34

        Sakhalin-Korean’s “permanent homecoming” has processed in the discourse on the post cold war since Korea-Russia establishment of national friendship in 1990. Economic needs for coal, oil, gas and fishery resources is not irrelevant. Busan has been emerging as a representative trading city on both sides of Korea and Russia. This thesis considers that economic interchange has relevance to people’s movement and contemplates Sakhalin-Korean’s homecoming on family relation in the view of post colonialism. Sakhalin-Korean had started moving to Sakhalin through Yeunhaeju(연해주), most of them were forced to move under Japanese imperialism and worked on coal mine or logging. They had been under detention until 1990’s when negotiation between Korea, Japan and Russia had been occurred. This was ‘business’ made by negotiation among countries in the mood of post colonialism, post cold war. It is ‘another migration’ for Sakhalin-Korean, and it causes another family separation. In other words, ‘double separation’ as return to Korea means that the first generation leave their home under colonial era, or family separation that occurred because of impressments to mainland Japan. It is paradoxical situation as parents generation’s wish for family reunion becomes separation from next generation. This is because firstly, only people who were born before 1945 and their spouses acquire qualification to come back their homeland. At the point of time, end of Japanese colonialism in 1945 is applied. This measure lose sight of cold war system that has stagnated return after the liberation. Secondly, people who returned are not considered as member of family foamed in Sakhalin. This instigates isolation, family segmentalization. This problem shows the limit of there turn that made inside the frame of nation state. In this particular situation, the returnees perform a role connecting Busan and Sakhalin. At this present 120 returnees have settled down mostly at close to their home town of their parents or themselves. Sakhalin returnees, mostly second generation attach great importance to ‘return home on behalf of parents’ and put an effort on relationship with their relatives in homeland. In addition, they have been creating another life world between Busan and Sakhalin by attaching a meaning of their new home and trying to keep in contact with remained family in Sakhalin.

      • 中學校 新ㆍ舊科學敎科書의 比較分析에 關한 硏究

        安美貞 건국대학교 교육대학원 1990 敎育論叢 Vol.14 No.-

        Comparing with the old and new science curriculum of middle school, analysing and comparing with a sort of science state textbook for each grade and five kinds of testbook,(which are newly revised and pubished), for 1,2,3-year-grade of official approval of textbooks : therefore, at the result of such analyses, We reached such a following conclusion : 1) The old and new curriculum have no great differences, but the curriculum of the new textbooks put the chief aim into fostering of the fundamental experiment and the skill of practice and the development of science-technology, and the recognition of the influence on the society development. 2) The divisional proportion of physical geography, biology, chemistry, was nearly similar. 3) Analysing the old and new science textbook, in the new textbook, the number of pages increased by 7.2%, of experiment decresed by 10%, of observation decreased by 41.2%, and of research deareased by17%, of table decreased by 28% of picture decreased 2%, of question increased by 71.5%, of things to be read increased 25%, and the oppendices are edited only in the textbook. 4) Within the limit of chemistry division, the small section among the contents of 2-year-grade are transferred into the curriculum of 3-year-grade. The grand unit (Chemistry reaction and heat is deleted. 5) The chemistry experiment the old and new textbook is all similar

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