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Phospholipase C를 투여한 랫트의 간장에서의 Cytochrome P450의 발현양상
지영흔,이차수,박청규,박상준,정원일,도선희,박승춘,류시윤,정규식 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.4
Alpha-toxin, produced in large amounts by Clostridium perfringens type A strain, is phospholipase C(PLC) and is believed to be the major factor responsible for several biological damages of animals and humans. We attempted to know the expressions of drug metabolism related enzymes, cytochrome P450s(P450s) and membrane lipid peroxidation product, 4-hydroxy-2-nonenal(HNE) after injection of PLC or purified alpha-toxin in Sprague-Dawley rats. The serum-biochemical value which is used as an indicator for major liver damages, activities of asparatate aminotransferase(AST) and alanine aminotransferase(ALT) after injection of PLC increased by 2-fold and 3-fold, respectively. Immunohistochemical staining for P450 1A1/2, 2B1/2, 2E1, 3A1 and 4A2/3 was performed in liver at various times after injection of PLC or alpha toxin. In all experimental groups, immunoreactivities of P450 2E1, 2B1/2, 1A1/2 and 4A2/3 were weak and the immunoreactivities of P450 2C11 were not detectable until 6hrs. Among them,P4503A1 showed strong immunoreactivity on pericentral area at 2hrs after PLC administration. Following a time course of 1∼24 hrs, HNE, a product of lipid peroxidation, was noted positive immunoreactivity on the peripheral area after PLC injection. In immunoblot analyses, the expressions of P450 2E1, 2B1/2, 1A1/2 and 4A2/3 slightly decreased while P450 2C11 significantly decreased until 6hrs. In contrast, HNE was significantly induced at 3.5hrs and increased expression of HNE persisted until 6hrs. In conclusion, the increased expression of HNE, a well known toxic product of the membrane lipid, indicated that PLC showed drug metabolizing enzyme via suicidal inhibitory effects and functions. So, these data support a membrane damaging effect of enzymatic impairment in pathological conditions of the lipid peroxidation.
머위(Petasites japonicus maxim)를 급여한 rat와 mouse에 대한 병리학적 관찰 I. 육안적 및 병리조직학적 관찰
지영흔,이차수,Jee, Young-heun,Lee, Cha-soo 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2
In order to know the toxic effect and carcinogenic activity in rats and mice fed with juice of Korean native Petasites japonicus Maxim of its pellet(4% or 8%) which were dried, milled and mixed with basal diet, the investigations were carried out by macroscopy and histopathology. Macroscopically, although remarkable changes were not observed in the liver of mice, there were slight to moderate swelling of rat livers in the whole groups at 12 to 14 weeks after feeding and milky spots in rats fed with its juice and 8% pelleted Petasites japonicus Maxim diet and a normal diet for 1 week alternatively for 14 weeks. Moreover, moderate to severe swelling and milk spots were recognized in livers of all rats fed with its juice and 8% pellet or 8% pelleted Petasites japonicus Maxim for 16 weeks. But, in cases of rats fed with its juice and 4% pellet or 4% pelleted Petasites japonicus Maxim, only swelling of livers was recognized moderately or severely. Histopathologically, major lesions were found in livers of both rats and mice. There were congestion, hemorrhage, fatty change, focal necrosis, megalocytosis and hyperplasia of endothelial cell in livers of mice and rats, the additional lesions such as proliferation of bile duct and nodular regeneration with diffuse regenerating cells were seen in livers of rats. In addition, preneoplastic lesions, the areas of milky spots macroscopically, were observed in livers of rats fed with Petasites japonicus Maxim for 14 to 16 weeks. In a few cases, haemangioendothelial sarcoma in livers was detected in rats fed with Petasites japonicus Maxim for 16 weeks. Petasites japonicus maxim growing naturally in Korea seem to exhibit toxic effect especially in liver and it contained a causative agent of primary liver tumors.
자가면역성 뇌척수염 랫드의 중추신경계에서의 인산화된 IkB의 발현양상
황인선,지영흔,Hwang, In Sun,Jee, Young Heun 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.1
To elucidate the roles of phospho-IkB expression in the development and progression of EAE, we investigated the expression of phospho-IkB in the central nervous system (CNS) of rats during experimental autoimmune encephalomyelitis (EAE) using immunohistochemistry and Western blot analysis. In Western blot analysis, the increased expression of phospho-IkB went parallel to severity of EAE. The expression of phospho-IkB increased significantly at the peak stage of EAE followed by gradual decrease. Immunohistochemical studies showed that the phospho-IkB immunoreactivity was mainly expressed in inflammatory cells (macrophages, T cells) and glial cells (astrocytes, microglial cells) at the peak stage of EAE and disappeared at the recovery stage. These findings suggest that the phosphorylation of IkB is closely associated with autoimmune inflammation in the CNS and plays an important role in the initiation and progression of EAE.
채집 시기에 따른 제주 연안 괭생이모자반의 외관 특성 및 항산화 특성
부하정,문효석,지영흔,전유진,천지연 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.5
본 연구에서는 괭생이모자반을 2019년 2월부터 6월까지 제주특별자치도 제주시 인근 연안에서 매월 채집하여 계절에 따른 외관 변화와 일반성분을 조사하고 추출 용매를 달리하여 총 폴리페놀 함량 및 항산화 활성을 측정하였다. 괭생이모자반은 계절에 따른 성장의 차이를 보였고 탄수화물이 주요 성분이며 높은 조단백질 함량을 가지고 있어 단백질의 공급원으로 활용 가능성이 높을 것으로 보인다. 추출물의 수율과 총당 함량은 열수 추출물이 유의적으로 높았으며, 추출물의 다양한 특성은 채집 시기에 따라 다르게 나타났다. 총 폴리페놀 함량, DPPH와 ABTS 라디칼 소거능은 70% 에탄올 추출물의 경우 5월에서 가장 높게 나타났다. 따라서 채집 시기 중 개체 수가 풍부하고 탄수화물 및 조단백질 함량이 높으며 항산화 활성이 좋은 5월이 채집 적기로 판단된다. 또한, 추출 수율과 총당 함량은 열수 추출물이 높았으나 이는 수용성 당이 다량 추출된 것으로 보이며, 총 폴리페놀 함량이 높고 항산화 활성이 좋은 70% 에탄올 추출물이 추출 용매로서 가장 적합하다. 본 연구는 괭생이모자반의 원재료로서의 특성을 채집 시기 및 추출조건에 따라 관찰함에 따라 향후 다양한 가공식품 및 건강기능성식품으로 개발 및 제조 시 유용한 정보가 될 것으로 판단한다. This study was conducted to investigate changes in the external morphological and chemical compositions of Sargassum horneri according to collection time as well as determine the total polyphenol content and antioxidant activity based on different extraction solvents. The carbohydrate contents of S. horneri according to month were 45.1∼59.3%, crude protein contents were 19.0∼26.2%, and crude lipid contents were 1.08∼2.85%. The extraction yields and polysaccharide contents of hot water extracts were significantly higher than those of ethanol extracts (P<0.05). The total polyphenol contents and 1,1-diphenyl-2-picrylhydrazyl and 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging activities were the highest in May for the 70% ethanol extracts. As a result, May was shown to be the most suitable month to collect S. horneri as a functional food material. In May, S. horneri constituted a large portion of the population and had higher carbohydrate and crude protein contents. Moreover, ethanol extracts had higher total polyphenol contents and antioxidant activities.
안미정,허승담,지영흔,주홍구,이용덕,심기범,신태균,Ahn, Mee-jung,Heo, Seung-dam,Jee, Young-heun,Joo, Hong-gu,Lee, Yong-duk,Sim, Ki-Bum,Shin, Tae-kyun The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.4
The phosphorylation of extracellular signal-regulated kinases (p-ERK) in the spinal cord of rats with acute monophasic experimental autoimmune encephalomyelitis (EAE) was studied using immunohistochemistry and treatment with inhibitor. P-ERK is constitutively expressed in glial cells in the normal spinal cord. In EAE, some inflammatory cells in the subarachnoid space were positive for p-ERK at the early stage, and its immunoreactivity declined when those cells infiltrated the parenchyma at the peak stage. In a blocking experiment using its inhibitor, the intravenous administration of PD98059 from day 7 to 13 post-immunization did not modulate EAE paralysis. Considering the results, we postulate that intravenous administration of PD98059 is not effective in ameliorating EAE paralysis, although many inflammatory cells express ERK in the subarachnoid space.
Caffeine이 N-bis(2-hydroxypropyl)nitrosamine과 sulfadimethoxine에 의해 유발된 갑상선 피막의 섬유성 증식에 미치는 영향
손화영,윤원기,지영흔,류시윤,김정란,조성환,Son, Hwa-young,Yoon, Won-kee,Jee, Young-heun,Ryu, Si-yoon,Kim, Jung-ran,Cho, Sung-whan 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.4
Caffeine (1,3,7-trimethylxanthine), a central nervous system stimulant, is contained in various foods, beverages and over-the-counter medications. Sulfadimethoxine (SDM) is one of the anti-thyroid agents and induces proliferation of thyroid capsule in two stage thyroid carcinogenesis model using N-bis(2-hydroxypropyl)nitrosamine (DHPN). In this study, we examined the effect of caffeine on fibrous proliferation of thyroid capsule in DHPN and SDM-treated rats. Five-week-old male F344 rats were given a single subcutaneous injection of DHPN (2,800 mg/kg, body weight). Starting one week thereafter, SDM (1,000 ppm in drinking water) with or without caffeine (1,500 ppm in diet) was administered for 12 weeks. All animals were autopsied and histopathological examination of the thyroid glands was performed. Thyroid follicular proliferative changes were induced in all rats treated with DHPN+SDM. In addition, the proliferation of perithyroidal fibrous tissue and pleomorphic thyroid follicular cells within the capsule were observed in DHPN+SDM treated group. Caffeine would not be related to these lesions in this experimental condition. although pentoxifylline, a methyl xanthine derivative, has an anti fibrotic effects.