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Transformed E . coli JM 107 에서 순수분리한 S . marcescens 세포외 Nuclease 의 특징
전영환,김기홍,권헌영,신용철,조무제,이상열 ( Young Hwan Jeon,Kee Hong Kim,Heun Young Kwon,Yong Chul Shin,Moo Je Cho,Sang Yeol Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5
We have previously described the purification procedures of the extracellular nuclease of Serratia marcescens from a recombinant E. coli JM 107 (Jeon and Lee, 1992). Using the purified nuclease, we examined the various biochemical properties of this enzyme. Kinetic parameters of thermal inactivation followed the first order kinetics. Heating the nuclease to 60℃ or higher temperature reduced enzymatic activity. It required 1 mM Mg^(2+) or Mn^(2+) ions for its maximal activity and no other metal ions can be replaced for the cofactor requirement of this enzyme. It was stable at pH in the range of pH 6 to 10, and its maximal activity was exhibited around pH 8. It was resistant to proteolysis by trypsin and an extracellular protease produced from S. marcescens. However, heat-denatured nuclease and bovine serum albumin were completely degraded when they were treated with the same proteases at the same assay conditions. Its activity was significantly inhibited by the addition of 1 mM ethidium bromide.
꽃송이버섯(Sparassis crispa) 추출물의 면역세포 활성화 및 항암 효과
김인규(In-Kyu Kim),윤영철(Young Chul Yun),신용철(Yong Chul Shin),유지윤(Jiyun Yoo) 한국생명과학회 2013 생명과학회지 Vol.23 No.8
꽃송이버섯(Sparassis crispa)은 항암 및 면역증강 효과가 뛰어나다고 알려져 있는 베타-글루칸의 함량이 건조중량의 40% 이상인 것으로 보고되고 있는 산림버섯이다. 일반적으로 버섯에서 추출한 베타-글루칸의 면역활성 및 항암 효과는 널리 알려져 있는 반면 꽃송이버섯 유래의 베타-글루칸에 의한 대식세포의 활성화 기작 및 자실체추출물에 의한 항암 효과는 아직 보고되지 않았다. 본 연구에서는 꽃송이버섯 유래 베타-글루칸의 면역활성 효과를 확인하고자 마우스의 대식세포인 Raw 264.7 cell을 이용하였으며, 베타-글루칸 처리시 TNF-α와 interleukin-1β(IL-1β)와 같은 사이토카인의 발현 및 분비가 증가됨을 확인하였고, 이러한 과정에 필수적인 역할을 담당하는 전사인자인 NF-kB가 활성화됨도 확인하였다. 뿐만 아니라 꽃송이버섯 추출물 처리 시 TNF-α의 분비가 증가됨과 함께 in vivo에서 암의 성장을 억제하는 기능이 있음을 확인하였다. 이러한 결과들은 꽃송이버섯이 면역활성을 증가시켜 암의 성장을 억제하는 건강 기능성 식품으로서의 개발 가능성이 높음을 의미하는 것이다. Sparassis crispa is an edible mushroom with medicinal properties that contains more than 40% β-glucan. The role of S. crispa in regulating the functional activation of macrophages has yet to be fully elucidated. The objective of this study was to investigate the molecular mechanism underlying the immune-stimulatory function of S. crispa soluble β-glucan and extracts on macrophages. In this study, we showed that S. crispa soluble β-glucan was able to stimulate TNF-α and IL-1β production through NF-kB activation in Raw 264.7 cells. We also showed that S. crispa extracts could not only enhance TNF-α production in Raw 264.7 cells, but also suppress tumor growth in vivo. All of our results suggest that S. crispa could be developed as a promising immunostimulatory principle, applicable to cancer patients.
ICCD Camera를 이용한 NO입자의 Image 관측
전용우(Yong-Woo Jun),최준영(Joon-Young Choe),최상태(Sang-Tae Choi),박원주(Won-Zoo Park),이광식(Kwang-Sik Lee),신용철(Yong-Chul Shin) 한국조명·전기설비학회 2000 한국조명·전기설비학회 학술대회논문집 Vol.2000 No.-
In this parer, the removal rate of NO in a reactor is measured first using nonthermal plasma generated from a wire-cylinder type reactor, then the spatial density distribution of NO particles investigated using ICCD(Intensified Charged Coupled Device) camera. This research uses nonthermal plasma from electrical discharge to analyze the NO characteristics, and the measurements of NO discharge image and Distribution are performed using the ICCD camera to examine the NO characteristics more closely. Furthermore . the method of Laser Induced Fluorescence (LIF) is used to analyze the particular behavior of NO particles more specifically, to suggest a method of reducing exhaust gas, a serious environmental problem.
미국 상위 30개 연구 중심 대학들의 실험실 환경보건안전 담당 부서 -MIT 사례를 중심으로-
안광석 ( Kwang Seog Ahn ),강동묵 ( Dong Mug Kang ),신용철 ( Yong Chul Shin ),전영희 ( Young Hee Jun ) 한국산업위생학회 2007 한국산업보건학회지 Vol.17 No.3
Objectives: The objectives of this research are to examine the missions, organization, and programs of the environmental, health and safety(EHS) offices of the major research universities in the U.S.A., particularly the case of Massachusetts Institute of Technology(MIT) EHS Office, and to suggest ways to address the EHS issues of the universities and research institutes in South Korea. Methods: The top 30 research universities in the U.S.A. were selected by the total amounts of research funds they annually received. The web sites of the 30 universities were searched to identify the names of the departments that address environment, health, and/or safety related issues, the missions of those departments, the major programs of those departments, and the number of staff in those departments. Also, a case study was conducted for the Environment Health and Safety Management System and the Environment, Health and Safety Office of the MIT, using literature and web searches and a meeting with the Director of the MIT Environment, Health and Safety Office. Results: All the top 30 research universities in the U.S.A. had designated departments that dealt with EHS issues. Most of them were by the name of or similar to environmental, health and safety. The mission statements of those departments were mostly about improving the safety and health of their community members, improving the environment, and complying to EHS regulations. Most of those departments had an environmental management program, industrial hygiene program, radiation protection program, safety program, and biosafety program as their major programs. The components of the environment, health and safety management system(EHS-MS) of the MIT were examined in detail. In contrast, not many universities in South Korea had designated departments that dealt specifically with EHS issues. Also, the number of fulltime staff for EHS was only 1-2 in most of the Korean universities and their work duties included only general safety, while neglecting other health/environment related issues. Conclusions: Well organized and functioning environmental, health and safety offices were present in all of the top 30 research universities in the U.S.A., whereas similar organizations of the universities in South Korea were virtually non-existent and/or had very limited EHS activities. Therefore, in order to reduce potential risks of accidents and health problems in the Korean universities and research institutions, well established and functioning EHS-MSs and EHS offices are warranted. The case of the EHS-MS and EHS Office in the MIT demonstrate a successful case to follow.
이공계열 전공 공부에 필요한 고등학교 물리 선수과목에 대한 대학생들의 인식에 대한 질적 탐구
곽영순 ( Youngsun Kwak ),조향숙 ( Hyang-suk Cho ),이일 ( Il Lee ),손미현 ( Mihyun Son ) 한국현장과학교육학회 2019 현장과학교육 Vol.13 No.4
본 연구의 목적은 고교-대학 연계성 제고를 위해 고등학교 학생들의 교과목 선택권을 실질적으로 보장하는 방안을 탐구하려는 것이다. 이를 위해 총10명의 이공계열 학부 3, 4학년 및 대학원 재학생을 추천받아 초점집단 심층면담을 실시하였으며 대학전공 공부를 위한 고교 물리 선수과목 이수의 필요성, 대학 전공공부에 필요한 물리 선수과목을 고등학교에서 이수하지 않은 이유, 물리 선수과목 이수를 고등학교 후배들에게 권장할 의사, 대학전공 공부에 필요한 고교 물리 선수과목 이수를 보장하는 방안 등을 탐구하였다. 연구결과 물리 과목의 필요성은 인지하고 있으나, 현실적 이유로 인해 물리과목을 기피하고 있으며, 이를 개선하기 위해서 고등학교 교육과정 편성 · 운영 정상화 방안을 소수 선택과목 개설 보장, 과학Ⅱ 과목 내신 및 수능 성적 산출 방식 개선, 고교 필수선수과목에 대한 이공계열별 진로 안내, 과학교사의 수업전문성 및 진로지도 전문성 제고, 이공계열 전공공부에 필요한 필수선수과목 이수를 보장하기 위한 대입전형 시스템을 마련의 필요성 등을 제안하였다. The purpose of this study is to explore ways to secure highschool students’ choice of courses in order to improve highschool - university linkage. For this purpose, we conducted in-depth focus group interviews with a total of 10 students in the second or the third year or graduate student at the science and engineering college, and these interviewees were recommended by their professors. Through interviews, we explored the necessity to complete highschool Physics prerequisite courses, reason for not completing highschool Physics prerequisite courses necessary for college major, their intention to recommend highschool juniors to complete Physics prerequisite courses, and ways to guarantee prerequisite course completion for college major studies. Based on the results, we suggested ways to link highschool - university curriculum for science & engineering majors. Discussed in the conclusion are ways to stabilize highschool curriculum organization and implementation such as opening elective courses with a few students without disadvantage in the school record, improving the grading system for Science Ⅱ courses in the school record as well as the College Scholastic Ability Test, providing career guidance about prerequisite courses by science & engineering majors, improving the science teacher’s professionalism on teaching and career guidance, and developing college entrance systems to guarantee the completion of prerequisite courses for science & engineering college studies.
모 선박용 디젤엔진 제조업체 들기작업의 인간공학 위험요인 평가
김부욱 ( Boo Wook Kim ),김선자 ( Sun Ja Kim ),신용철 ( Young Chul Shin ),김현동 ( Hyun Dong Kim ),우지훈 ( Ji Hoon Woo ),강동묵 ( Dong Mug Kang ),이현석 ( Hyun Seok Lee ) 한국산업위생학회 2005 한국산업보건학회지 Vol.15 No.3
The purpose of this study was to assess the ergonomic risks of lifting tasks in a marine diesel engine manufacturing industry using the National Institute for Occupational Safety and Health(NIOSH) Revised Lifting Equation(NLE). Average Lifting Index(LI=Weight of Load/Recommended Weight Limit) of a total number of 45 lifting tasks was 1.6±0.7. The LIs were above 1 at 34 tasks(75.6%), and above 2 at 11 tasks(24.4%). Parts management showed the highest average LI value (LI=2.3) in all departments, which resulted from high frequency and heave load of lifting. The common and significant ergonomic risk factors in the processes were the heavy weight of diesel engine parts and the long horizontal distance. In addition, some lifting tasks had such potential risk factors as the long vertical distance, the high frequency of lifts or the long work duration.