始南齋의 扁額

서흥식 전라금석문연구회 2010 전라금석문연구 Vol.15 No.-

고창 고수지역의 정자

서흥식 전라금석문연구회 2009 전라금석문연구 Vol.14 No.-

Assessment of single oral dose toxicity of collagen peptide from skate (Raja kenojei) skin

문창종,서흥식,박명규,성낙원,강근희,김상호,김중선,김성호,김종춘 충북대학교 동물의학연구소 2023 Journal of Biomedical and Translational Research Vol.24 No.3

Collagen peptides have garnered significant attention as functional foods across multiple fields due to their capacity to regulate physiological and hormonal processes, offering numerous advantages. However, despite their broad range of applications, comprehensive research on the potential toxicity of these substances remains lacking. Therefore, this study sought to assess the acute oral toxicity of a collagen peptide derived from skate (Raja kenojei) skin (CPSS) in both rats and dogs. In the rat model, CPSS was orally administered at doses of 300 and 2,000 mg/kg to Sprague-Dawley rats. An escalating single-dose oral toxicity assessment at doses of 500, 1,000, and 2,000 mg/kg was carried out in beagle dogs with 3-day intervals between doses. Throughout the 14-day post-administration assessment period, clinical signs, mortality rates, changes in body weight, and necropsy observations were closely monitored. After oral administration, no signs of toxicity associated with CPSS were observed in either rats or dogs. Therefore, the oral LD50 (approximate lethal dose for 50% mortality) for CPSS in rats was determined to exceed 5,000 mg/kg, and the maximum tolerated dose for dogs was estimated to be above 2,000 mg/kg. Consequently, this study offers safety data on the use of CPSS in functional foods and medicinal applications.

Twenty-eight-day oral dose toxicity evaluation of SUNACTIVE Fe-P80 in rats

김종춘,박명규,서흥식,김상호,김웅일,신인식 충북대학교 동물의학연구소 2022 Journal of Biomedical and Translational Research Vol.23 No.4

Iron deficiency is known to be a common nutritional disorder in many countries, especially among children, women of childbearing age and pregnant women. SUNACTIVE Fe-P80 is a new type of iron supplement that applies nanotechnlateology for the purpose of overcoming the disadvantages of food supplements. This study was conducted to investigate the potential adverse effects of a 28-day repeated oral dose of SUNACTIVE Fe-P80 in rats. SUNACTIVE Fe-P80 was administered once daily by gavage to Sprague-Dawley rats for 28 days at doses of 0, 500, 1,000, and 2,000 mg/kg/day. Additional recovery groups from the control and highdose groups were observed for a 14-day recovery period. At the scheduled termination, the animals were sacrificed, their organs weighed, and blood samples collected. There were no treatment-related effects in the context of clinical signs, body weight, food intake, ophthalmoscopy, urinalysis, necropsy findings, organ weights, and hematologic, serum biochemical and histopathological parameters at any dose tested. Under the present experimental conditions, the no-observed-adverse-effect level of SUNACTIVE Fe-P80 was ≥ 2,000 mg/kg/day in both the sexes, and no target organs were identified. Thus, the results suggest that SUNACTIVE Fe-P80 is relatively safe, as no treatment-related adverse effects were observed following a 28-day repeated oral dose experiment.

부인암 환자에서 방사선치료 후 발생한 합병증에 관한 연구

진건,박충학,고경심,윤형근,서흥식,박혜영,오현양 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.8

1994년 5월부터 1997년 7월까지 단국대학교병원에서 부인암으로 방사선치료를 받은 72명의 환자를 대상으로 방사선치료 후 발생한 합병증을 비교 분석하였다. 급성 합병증은 총 72명 중 20명(27.7%)(Upper GI toxi city; 1, Lower GI toxicity; 13, GU toxicity; 6)에서 발생했 는데 합병증 발생 빈도를 비교 분석한 결과는 다음과 같았다. 1. 방사선 총조사량이 45 Gy 미만인 경우(14.3%) 보다 방사선 총조사량이 45 Gy 이상인 경우(29.2%)에서 합병 증 발생 빈도가 더 높았으나 통계적으로 유의한 차이를 보이지는 않았다. 2. 외부 방사선치료만 받은 경우(25.0%) 보다 외부 방 사선치료와 강내 근거리요법을 병행해서 치료 받은 경 우(28.8%)에서 합병증 발생 빈도가 더 높았으나 통계적으로 유의한 차이를 보이지는 않았다. 3. 60세 미만인 경우(25.6%) 보다 60세 이상인 경우 (31.0%) 합병증 발생 빈도가 더 높았으나 통계적으로 유 의한 차이를 보이지는 않았다. 4. 내과적 병력이 없는 경우(27.4%) 보다 내과적 병력이 있는 경우(30.0%)에서 합병증 발생 빈도가 더 높았으나 통계적으로 유의한 차이를 보이지는 않았다(Table 8). 만성 합병증은 총 66명 중 18명(27.2%)(Lower GI toxi city; 11, GU toxicity; 7)에서 발생했는데 합병증 발생 빈 도를 비교분석한 결과는 다음과 같았다. 1. 방사선 총 조사량이 45 Gy 미만인 경우(14.2%) 보 다 방사선 총 조사량이 45 Gy 이상인 경우(28.8%)에서 합병증 발생 빈도가 더 높았으나 통계적으로 유의한 차이를 보이지는 않았다. 2. 외부 방사선치료만을 받은 경우(22.2%) 보다 외부 방사선치료와 강내 근거리요법을 병행해서 받은 경우 (29.2%)에서 합병증 발생 빈도가 더 높았으나 통계적으로 유의한 차이를 보이지는 않았다. 3. 60세 미만인 경우(30.8%) 보다 60세 이상인 경우 (22.2%)에서 합병증 발생 빈도가 더 낮았으나 통계적으 로 유의한 차이를 보이지는 않았다. 4. 내과적 병력이 없는 경우(25.4%) 보다 내과적 병력 이 있는 경우(42.9%)에서 합병증 발생 빈도가 더 높았으나 통계적으로 유의한 차이를 보이지는 않았다(Table 9). 이상의 결과로 부인암 환자에서 방사선치료 후 급성 합병증과 만성 합병증이 흔하다는 것을 알 수 있었으며, 특히 방사선 총조사량이 많은 환자군, 외부 방사선치료와 강내 근거리요법을 병행해서 치료받은 환자군, 내과 적 병력이 있는 환자군에서 더 많았으나 통계적으로 유 의한 차이를 보이지는 않았다. 그러나 대부분의 합병증 이 grade 2 이하의 경증 합병증이었고 grade 3 이상의 중 증 합병증은 2명(2.8%)(Acute GI toxicity; 2)에서 발생 했다. 정확한 만성 합병증의 발생 빈도를 연구하기 위해서는 추적 관찰 기간이 길어야 하므로 향후 지속적인 연구 가 있어야 할 것이다. 급성과 만성 합병증 모두에서 하부 위장관계 합병증이 가장 많았으므로 향후 하부 위장관계 합병증을 줄이는데 노력해야 하겠다. Objective; The purpose of this study was to determine the frequency and severity of complications following pelvic irradiation in patients with gynecologic malignancies. Study Design; A retrospective analysis was done in 72 patients treated with pelvic radiation therapy for cervical (N=66) and endometrial (N=6) cancer at Dankook University Hospital from May 1994 to July 1997. Results; The complications of pelvic irradiation were as follows; 1. Acute gastrointestinal complications occured in 14 of 72 patients (19.4%) (mild diarrhea; 5, hematochezia; 4, mild nausea and vomiting; 1, moderate diarrhea; 2, severe diarrhea; 2). 2. Acute genitourinary complications occured in 6 of 72 patients (6.3%) (cystitis; 6). 3. Chronic gastrointestinal complications occured in 11 of 66 patients (16.7%) (mild diarrhea; 5, hematochezia; 4, mild small bowel obstruction; 1, moderate diarrhea; 1). 4. Chronic genitourinary complications occured in 7 of 66 patients (10.6%) (cystitis; 6, gross hematuria; 1). 5. The incidences of acute and chronic complications after pelvic irradiation in gynecologic malignancies were higher in the following groups; 1) The group with total dose of external radiation over 45Gy. 2) The group with external beam radiation and brachytherapy modality. 3) The group of patients with medical problems. But there were no statistical significant differences in these groups. Conclusion; We concluded that complications are common after pelvic radiation therapy. Pelvic radiation therapy should be used judiciously for the purpose of reducing complications, particularly in the patients with higher dose radiation therapy and pre-existing medical problems.

유년형 낭성 난소과립막세포종 1예

박혜영,김동희,박충학,오현양,서흥식,고재향 대한부인종양 콜포스코피학회 1999 Journal of Gynecologic Oncology Vol.10 No.1

Sex cord-stromal tumors of the ovary are the third most common types of neoplasms that develop in the ovary and account for about 5-8% of all ovarian malignancies. This group of ovarian neoplasms is derived from the sex cords and the ovarian stroma or mesenchyme. Granulosa-stromal cell tumors include granulosa cell tumor, thecoma and fibroma. The granulosa cell tumor is a low-grade malignancy and accounts for about 2-3% of all ovarian malignancies. Granulosa cell tumors have a prolonged natural history and tendency toward late relapse, reflecting their low grade biology. We present a case of juvenile granulosa cell tumor of the ovary with brief review of literature.

CO2 레이저와 인터페론 알파-2b로 병합치료한 질에 발생한 다발성 첨형 콘딜로마 1 예

진건,박충학,고경심,서흥식,박혜영,오현양,박향준 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.6

저자 등은 1997년 6월 단국대학교병원 산부인과에 내원한 55세의 경산부에서 자궁경부와 질에 발생한 다발성 첨형 콘딜로마를 CO2 레이저와 인터페론 알파-2b로 병합치료하여 완치시켰기에 문헌고찰과 함께 보고하였다. Condyloma acuminatum is a contagious viral infection caused by types 6 and 11 human papillomavirus[HPV], but may also be caused by types 16, 18, 30s group, 40s group, 50s group, and 60s group. The warts tend to occur most directly by coitus. Treatment of vulvar condyloma ranges from topical podophyllin, trichloroacetic acid, and 5-fluorouracil application to local excision, cryotherapy, electrosurgery, and carbon dioxide laser vaporization. The goal of treatment is removal of the warts;it is not possible to eradicate the viral infection. We have experienced one case of multiple condyloma acuminata in the vagina treated with carbon dioxide laser vaporization combined with intralesionally injected interferon alfa-2b, which is presented with a brief review of the literatures.

자외선 B 조사 마우스에서 피부손상에 대한 보중익기탕의 효과

김중선,이해준,송명섭,서흥식,문창종,김종춘,배춘식,조성기,김성호,Kim, Joong-Sun,Lee, Hae-June,Song, Myoung-Sub,Seo, Heung-Sik,Moon, Changjong,Kim, Jong-Choon,Bae, Chun-Sik,Jo, Sung-Kee,Kim, Sung-Ho 대한수의학회 2009 大韓獸醫學會誌 Vol.49 No.1

The effect of Bu-Zhong-Yi-Qi-Tang (BZYQT) on the changes of ultraviolet (UV) light B radiation-induced apoptotic sunburn cell (SBC) and epidermal ATPase-positive dendritic cell (DC) in SKH1- hr or ICR mouse were investigated. The mice were treated with UVB (200 mJ/$cm^2$) and were sacrificed 24 h later. BZYQT (50 mg/kg of body weight) or vehicle (saline) was given i.p. at 36 and 12 h before irradiation, and 30 min after irradiation or BZYQT cream (0.2%) or cream base (vehicle) was topically treated at 24 h and 15 min before irradiation, and immediately after irradiation. The skin of SKH1-hr mouse prepared from the back of untreated mice exhibited about 0.3 SBC/cm length of epidermis, and 24 h after UV irradiation, the applied areas show an increased number of SBCs. But the frequency of UVB-induced SBC formation was reduced by intraperitoneal injection of BZYQT extract (p < 0.01). The numbers of DC in normal ICR mouse were 628.00 ${\pm}$ 51.56 or 663.20 ${\pm}$ 62.58 per $mm^2$ of ear epidermis. By 1 day after UVB treatment, the number of ATPase-positive cells/$mm^2$ were decreased by 39.0% or 27.1% in i.p. or topical application group with vehicle. Treatment of BZYQT was associated with increase of 33.9% in i.p. group (p < 0.05) or 2.7% in topical application group in the number of ATPase positive cells compared with the irradiation control group. The results presented herein that BZYQT administration could reduce the extent of skin damages produced by UVB.

고연성 무전해 Ni-P 도금액 개발

이성준,Nguyen Van Phuong,박준영,서흥식,김동현 한국표면공학회 2018 한국표면공학회 학술발표회 초록집 Vol.2018 No.6

GLP 수준에서의 생물농축성 시험법 소개

민석기,권회군,송은희,이유진,남지윤,서흥식,홍승국,김수현 한국농약과학회 2016 한국농약과학회 학술발표대회 논문집 Vol.2016 No.4